Restriction map for
pFN21AB7702
(Circular) (Six-base) MAPSORT of: pFN21AB7702.seq Check: 3221 from: 1 to: 6088
pFN21AB7702 6088 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2482 2838 2482
Size: 356 5732
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3431 1937
Size: 1494 4594
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3118 4151 5535 5803 1632
Size: 1486 1033 1384 268 1917
Fragments arranged by size:
1917 1486 1384 1033 268
AfeI AGC'GCT
Cuts at: 2195 2195
Size: 6088
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 2090 2145 4987 5964 1203
Size: 887 55 2842 977 1327
Fragments arranged by size:
2842 1327 977 887 55
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 2062 4817 2062
Size: 2755 3333
AleI CACnn'nnGTG
Cuts at: 1166 2848 1166
Size: 1682 4406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2866 2990 3096 3356 3371 1184
Size: 1682 124 106 260 15 3901
Fragments arranged by size:
3901 1682 260 124 106 15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 6056
AlwNI CAG_nnn'CTG
Cuts at: 2695 3064 3259 3262 3831 5403 2695
Size: 369 195 3 569 1572 3380
Fragments arranged by size:
3380 1572 569 369 195 3
ApaI G_GGCC'C
Cuts at: 2317 2376 2503 2667 2731 3289 2317
Size: 59 127 164 64 558 5116
Fragments arranged by size:
5116 558 164 127 64 59
ApaLI G'TGCA_C
Cuts at: 2149 4145 5301 2149
Size: 1996 1156 2936
Fragments arranged by size:
2936 1996 1156
ApoI r'AATT_y
Cuts at: 1493 3248 3397 3623 3775 5884 1493
Size: 1755 149 226 152 2109 1697
Fragments arranged by size:
2109 1755 1697 226 152 149
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 3413 1870
Size: 73 1470 4545
Fragments arranged by size:
4545 1470 73
BamHI G'GATC_C
Cuts at: 3418 3785 3418
Size: 367 5721
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2386 2528 3409 4864 618
Size: 325 217 627 599 142 881 1455 1842
Fragments arranged by size:
1842 1455 881 627 599 325 217 142
BanII G_rGCy'C
Cuts at: 1391 2317 2376 2451 2503 2652 2667 2682 2731
Size: 926 59 75 52 149 15 15 49
Cuts at: 2731 2757 3029 3289 3407 1391
Size: 26 272 260 118 4072
Fragments arranged by size:
4072 926 272 260 149 118 75 59
52 49 26 15 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2851 928
Size: 971 952 4165
Fragments arranged by size:
4165 971 952
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2368 2402 4279 4313 1903
Size: 34 431 34 1877 34 3678
Fragments arranged by size:
3678 1877 431 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3020 4699 136
Size: 107 122 71 631 605 1348 1679 1525
Fragments arranged by size:
1679 1525 1348 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6083 6083
Size: 6088
BlpI GC'TnA_GC
Cuts at: 3505 3505
Size: 6088
Bme1580I G_kGCm'C
Cuts at: 1790 2045 2153 2317 2376 2391 2503 2526 2533
Size: 255 108 164 59 15 112 23 7
Cuts at: 2533 2667 2731 2845 3289 4149 5305 1790
Size: 134 64 114 444 860 1156 2573
Fragments arranged by size:
2573 1156 860 444 255 164 134 114
112 108 64 59 23 15 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6088
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2495 2848 3819
Size: 416 53 83 399 24 1078 353 971
Cuts at: 3819 4777 442
Size: 958 1753
Fragments arranged by size:
1753 1078 971 958 416 399 353 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2384 2679 2723 2891 2929 4748 1239
Size: 1145 295 44 168 38 1819 2579
Fragments arranged by size:
2579 1819 1145 295 168 44 38
Bpu10I CC'TnA_GC
Cuts at: 2445 2676 3183 3290 2445
Size: 231 507 107 5243
Fragments arranged by size:
5243 507 231 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3101 4213 5078 5376 3101
Size: 1112 865 298 3813
Fragments arranged by size:
3813 1112 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4751 882
Size: 617 3252 2219
Fragments arranged by size:
3252 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2091 2148 2419 3879 493
Size: 711 887 57 271 1460 2702
Fragments arranged by size:
2702 1460 887 711 271 57
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3895 4277 4912 275
Size: 53 83 186 936 2362 382 635 1451
Fragments arranged by size:
2362 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2410 4521 4919 5193 5340 5751 2410
Size: 2111 398 274 147 411 2747
Fragments arranged by size:
2747 2111 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3299 3329 1178
Size: 30 165 30 1896 30 3937
Fragments arranged by size:
3937 1896 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6088
BseYI C'CCAG_C
Cuts at: 2429 2477 2503 5291 5787 2429
Size: 48 26 2788 496 2730
Fragments arranged by size:
2788 2730 496 48 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 2821 3020 1163
Size: 573 411 674 199 4231
Fragments arranged by size:
4231 674 573 411 199
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2153 2682 4149 4234 5305 2153
Size: 529 1467 85 1071 2936
Fragments arranged by size:
2936 1467 1071 529 85
BsiWI C'GTAC_G
Cuts at: 2016 2634 2016
Size: 618 5470
BsmI GAATG_Cn'
Cuts at: 3599 3692 3599
Size: 93 5995
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2153 2317 2376 2391 2451 2503
Size: 399 255 108 164 59 15 60 52
Cuts at: 2503 2526 2533 2652 2667 2682 2731 2757 2845
Size: 23 7 119 15 15 49 26 88
Cuts at: 2845 3029 3289 3407 4149 4234 5305 1391
Size: 184 260 118 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 260 255 184 164
119 118 108 88 85 60 59 52
49 26 23 15 15 15 7
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 3977 5707 3977
Size: 1730 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2482 2838 3363 3443 844
Size: 1638 356 525 80 3489
Fragments arranged by size:
3489 1638 525 356 80
BsrBI CCG'CTC
Cuts at: 1394 1823 3975 1394
Size: 429 2152 3507
Fragments arranged by size:
3507 2152 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4583 4757 65
Size: 1183 3335 174 1396
Fragments arranged by size:
3335 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2465 2779 3096 4732 4919 1700
Size: 110 655 314 317 1636 187 2869
Fragments arranged by size:
2869 1636 655 317 314 187 110
BsrGI T'GTAC_A
Cuts at: 96 2298 96
Size: 2202 3886
BssHII G'CGCG_C
Cuts at: 1925 2254 1925
Size: 329 5759
BssSI C'ACGA_G
Cuts at: 4148 5160 4148
Size: 1012 5076
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2695 3262 794
Size: 1901 567 3620
Fragments arranged by size:
3620 1901 567
BstBI TT'CG_AA
Cuts at: 4892 4892
Size: 6088
BstEII G'GTnAC_C
Cuts at: 1184 2980 1184
Size: 1796 4292
BstXI CCAn_nnnn'nTGG
Cuts at: 2458 2458
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 3418 3785 4170 4187 5639 5725 5737 6083
Size: 1446 367 385 17 1452 86 12 346
Cuts at: 6083 1972
Size: 1977
Fragments arranged by size:
1977 1452 1446 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2514 2533 513
Size: 552 939 510 19 4068
Fragments arranged by size:
4068 939 552 510 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5349
Fragments arranged by size:
5349 683 56
BtsI GCAGTG_nn'
Cuts at: 2507 3235 3604 4398 4418 2507
Size: 728 369 794 20 4177
Fragments arranged by size:
4177 794 728 369 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DraIII CAC_nnn'GTG
Cuts at: 3222 3222
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5095 809
Size: 507 3779 1802
Fragments arranged by size:
3779 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2275 2460 2517
Size: 54 1425 50 275 18 445 185 57
Cuts at: 2517 2572 2808 2877 3012 3535 4424 6053 8
Size: 55 236 69 135 523 889 1629 43
Fragments arranged by size:
1629 1425 889 523 445 275 236 185
135 69 57 55 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3137 4018 1353
Size: 1784 881 3423
Fragments arranged by size:
3423 1784 881
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4675 5049 5195 387
Size: 1064 138 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2680 2680
Size: 6088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2384 2679 2723 2891 2929 3118 4151
Size: 393 752 295 44 168 38 189 1033
Cuts at: 4151 4748 5535 5803 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1033 787 752 597 393 295 268
189 168 44 38
EcoNI CCTnn'n_nnAGG
Cuts at: 3275 3275
Size: 6088
EcoO109I rG'GnC_Cy
Cuts at: 2026 2314 2372 2373 2455 2499 2500 2663 3286
Size: 288 58 1 82 44 1 163 623
Cuts at: 3286 2026
Size: 4828
Fragments arranged by size:
4828 623 288 163 82 58 44 1
1
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 6088
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 2197 5235 1678
Size: 519 3038 2531
Fragments arranged by size:
3038 2531 519
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 4729 4761 4803
Size: 32 135 32 476 32 2649 32 42
Cuts at: 4803 4835 1373
Size: 32 2626
Fragments arranged by size:
2649 2626 476 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3432 3678 669
Size: 988 281 1494 246 3079
Fragments arranged by size:
3079 1494 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5202 5386 1305
Size: 3897 184 2007
Fragments arranged by size:
3897 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3014 10
Size: 54 1425 343 1182 3084
Fragments arranged by size:
3084 1425 1182 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2519 2848 3170 3365 4046 4405 4564
Size: 648 1353 329 322 195 681 359 159
Cuts at: 4564 518
Size: 2042
Fragments arranged by size:
2042 1353 681 648 359 329 322 195
159
MspA1I CmG'CkG
Cuts at: 1606 2692 2701 3076 3091 3259 3504 4181 5329
Size: 1086 9 375 15 168 245 677 1148
Cuts at: 5329 5574 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1086 677 375 245 245 168
15 9
NaeI GCC'GGC
Cuts at: 2781 2781
Size: 6088
NcoI C'CATG_G
Cuts at: 513 1065 2004 2514 513
Size: 552 939 510 4087
Fragments arranged by size:
4087 939 552 510
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NgoMIV G'CCGG_C
Cuts at: 2779 2779
Size: 6088
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 3298 3446 4991 1132
Size: 97 2069 148 1545 2229
Fragments arranged by size:
2229 2069 1545 148 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6088
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2477 2546 2624 3169 1108
Size: 1369 69 78 545 4027
Fragments arranged by size:
4027 1369 545 78 69
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PmlI CAC'GTG
Cuts at: 2091 2148 2091
Size: 57 6031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2026 2026
Size: 6088
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PspOMI G'GGCC_C
Cuts at: 2313 2372 2499 2663 2727 3285 2313
Size: 59 127 164 64 558 5116
Fragments arranged by size:
5116 558 164 127 64 59
PstI C_TGCA'G
Cuts at: 830 2691 2700 2751 2901 3075 3090 3440 830
Size: 1861 9 51 150 174 15 350 3478
Fragments arranged by size:
3478 1861 350 174 150 51 15 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
PvuII CAG'CTG
Cuts at: 2692 2701 3076 3091 3259 2692
Size: 9 375 15 168 5521
Fragments arranged by size:
5521 375 168 15 9
SacI G_AGCT'C
Cuts at: 2682 2682
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SbfI CC_TGCA'GG
Cuts at: 2751 3440 2751
Size: 689 5399
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SexAI A'CCwGG_T
Cuts at: 2718 2718
Size: 6088
SfcI C'TryA_G
Cuts at: 826 1045 2687 2696 2747 2897 3071 3086 3436
Size: 219 1642 9 51 150 174 15 350
Cuts at: 3436 4571 5252 5443 826
Size: 1135 681 191 1471
Fragments arranged by size:
1642 1471 1135 681 350 219 191 174
150 51 15 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3116 4192 5093 5355 820
Size: 197 926 1173 1076 901 262 1553
Fragments arranged by size:
1553 1173 1076 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3298 3446 1132
Size: 2166 148 3774
Fragments arranged by size:
3774 2166 148
SspI AAT'ATT
Cuts at: 5 52 4012 5
Size: 47 3960 2081
Fragments arranged by size:
3960 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6088
StyI C'CwwG_G
Cuts at: 513 1065 2004 2451 2514 3303 3527 513
Size: 552 939 447 63 789 224 3074
Fragments arranged by size:
3074 939 789 552 447 224 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3032 4115 4132 4285 4470 4915 325
Size: 1255 1452 1083 17 153 185 445 1498
Fragments arranged by size:
1498 1452 1255 1083 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2298 4334 6046
Size: 274 80 33 51 1443 321 2036 1712
Cuts at: 6046 96
Size: 138
Fragments arranged by size:
2036 1712 1443 321 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2024 2056 6048 6080 2024
Size: 32 3992 32 2032
Fragments arranged by size:
3992 2032 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6088
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 4217 4217
Size: 6088
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI BsmBI BstZ17I
Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PfoI PspXI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI