Restriction map for
pFN21AB7696
(Circular) (Six-base) MAPSORT of: pFN21AB7696.seq Check: 1068 from: 1 to: 5947
pFN21AB7696 5947 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3757 278
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3290 1937
Size: 1353 4594
Acc65I G'GTAC_C
Cuts at: 3268 3268
Size: 5947
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2650 4010 5394 5662 1632
Size: 1018 1360 1384 268 1917
Fragments arranged by size:
1917 1384 1360 1018 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5750
AflIII A'CryG_T
Cuts at: 1203 2343 4846 5823 1203
Size: 1140 2503 977 1327
Fragments arranged by size:
2503 1327 1140 977
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 4676 4676
Size: 5947
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5947
AlwNI CAG_nnn'CTG
Cuts at: 3690 5262 3690
Size: 1572 4375
ApaI G_GGCC'C
Cuts at: 2147 2686 2147
Size: 539 5408
ApaLI G'TGCA_C
Cuts at: 4004 5160 4004
Size: 1156 4791
ApoI r'AATT_y
Cuts at: 1493 3256 3482 3634 5743 1493
Size: 1763 226 152 2109 1697
Fragments arranged by size:
2109 1763 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4501 160
Size: 872 3469 1606
Fragments arranged by size:
3469 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 2200 3272 1870
Size: 73 257 1072 4545
Fragments arranged by size:
4545 1072 257 73
BamHI G'GATC_C
Cuts at: 2512 3277 3644 2512
Size: 765 367 4815
Fragments arranged by size:
4815 765 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2047 2080 2108 3268 4723
Size: 325 217 627 260 33 28 1160 1455
Cuts at: 4723 618
Size: 1842
Fragments arranged by size:
1842 1455 1160 627 325 260 217 33
28
BanII G_rGCy'C
Cuts at: 1391 2147 2574 2686 3266 1391
Size: 756 427 112 580 4072
Fragments arranged by size:
4072 756 580 427 112
BbeI G_GCGC'C
Cuts at: 2084 2112 2084
Size: 28 5919
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4976
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4138 4172 1903
Size: 34 2201 34 3678
Fragments arranged by size:
3678 2201 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3839 5049 3839
Size: 1210 4737
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5947
BfrBI ATG'CAT
Cuts at: 2604 2604
Size: 5947
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 2142 2168
Size: 107 122 71 631 605 406 64 26
Cuts at: 2168 2187 2196 4558 136
Size: 19 9 2362 1525
Fragments arranged by size:
2362 1525 631 605 406 122 107 71
64 26 19 9
BglII A'GATC_T
Cuts at: 5942 5942
Size: 5947
BlpI GC'TnA_GC
Cuts at: 3364 3364
Size: 5947
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2094 2147 2686 4008 5164 1790
Size: 262 42 53 539 1322 1156 2573
Fragments arranged by size:
2573 1322 1156 539 262 53 42
BmgBI CAC'GTC
Cuts at: 1318 2583 2800 1318
Size: 1265 217 4465
Fragments arranged by size:
4465 1265 217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3678 4636 442
Size: 416 53 83 399 24 2261 958 1753
Fragments arranged by size:
2261 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2770 4607 1239
Size: 1531 1837 2579
Fragments arranged by size:
2579 1837 1531
Bpu10I CC'TnA_GC
Cuts at: 2336 2336
Size: 5947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2894 2933 4072 4937 5235 2894
Size: 39 1139 865 298 3606
Fragments arranged by size:
3606 1139 865 298 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2990 4610 882
Size: 617 1491 1620 2219
Fragments arranged by size:
2219 1620 1491 617
BsaAI yAC'GTr
Cuts at: 493 1204 2025 2289 2346 3738 493
Size: 711 821 264 57 1392 2702
Fragments arranged by size:
2702 1392 821 711 264 57
BsaBI GATnn'nnATC
Cuts at: 5941 5941
Size: 5947
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2081 2109 2129 3754
Size: 53 83 186 936 548 28 20 1625
Cuts at: 3754 4136 4771 275
Size: 382 635 1451
Fragments arranged by size:
1625 1451 936 635 548 382 186 83
53 28 20
BsaWI w'CCGG_w
Cuts at: 2358 2540 4380 4778 5052 5199 5610 2358
Size: 182 1840 398 274 147 411 2695
Fragments arranged by size:
2695 1840 411 398 274 182 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5722
Fragments arranged by size:
5722 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3115 3118 1186
Size: 1929 3 4015
Fragments arranged by size:
4015 1929 3
BseYI C'CCAG_C
Cuts at: 2146 2475 5150 5646 2146
Size: 329 2675 496 2447
Fragments arranged by size:
2675 2447 496 329
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2471 2903 1163
Size: 573 735 432 4207
Fragments arranged by size:
4207 735 573 432
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2175 2854 3397 4158 5186 1540
Size: 275 186 174 679 543 761 1028 2301
Fragments arranged by size:
2301 1028 761 679 543 275 186 174
BsiHKAI G_wGCw'C
Cuts at: 2786 4008 4093 5164 2786
Size: 1222 85 1071 3569
Fragments arranged by size:
3569 1222 1071 85
BsmI GAATG_Cn'
Cuts at: 2776 3458 3551 2776
Size: 682 93 5172
Fragments arranged by size:
5172 682 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2431 3127 2431
Size: 696 5251
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2094 2147 2574 2686 2786 3266
Size: 399 262 42 53 427 112 100 480
Cuts at: 3266 4008 4093 5164 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 480 427 399 262 112
100 85 53 42
BspEI T'CCGG_A
Cuts at: 2540 5610 2540
Size: 3070 2877
BspHI T'CATG_A
Cuts at: 3836 5566 3836
Size: 1730 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2718 3302 844
Size: 1874 584 3489
Fragments arranged by size:
3489 1874 584
BsrBI CCG'CTC
Cuts at: 1394 1823 2265 2809 3834 1394
Size: 429 442 544 1025 3507
Fragments arranged by size:
3507 1025 544 442 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2016 4442 4616 65
Size: 1183 768 2426 174 1396
Fragments arranged by size:
2426 1396 1183 768 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2174 2822 4591 4778 1700
Size: 110 364 648 1769 187 2869
Fragments arranged by size:
2869 1769 648 364 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5947
BssHII G'CGCG_C
Cuts at: 1925 2563 1925
Size: 638 5309
BssSI C'ACGA_G
Cuts at: 4007 5019 4007
Size: 1012 4935
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2394 2601 794
Size: 1600 207 4140
Fragments arranged by size:
4140 1600 207
BstBI TT'CG_AA
Cuts at: 4751 4751
Size: 5947
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5947
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5947
BstYI r'GATC_y
Cuts at: 1972 2512 2883 3277 3644 4029 4046 5498 5584
Size: 540 371 394 367 385 17 1452 86
Cuts at: 5584 5596 5942 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 540 394 385 371 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2485 513
Size: 552 939 481 3975
Fragments arranged by size:
3975 939 552 481
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2369 487
Size: 683 56 1143 4065
Fragments arranged by size:
4065 1143 683 56
BtsI GCAGTG_nn'
Cuts at: 2786 3463 4257 4277 2786
Size: 677 794 20 4456
Fragments arranged by size:
4456 794 677 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 3251 3251
Size: 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2858 4954 809
Size: 507 1542 2096 1802
Fragments arranged by size:
2096 1802 1542 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2134 2172 2179
Size: 54 1425 50 275 18 304 38 7
Cuts at: 2179 2278 2649 2814 3227 3394 4283 5912 8
Size: 99 371 165 413 167 889 1629 43
Fragments arranged by size:
1629 1425 889 413 371 304 275 167
165 99 54 50 43 38 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 2172 3394 1537
Size: 275 360 1222 4090
Fragments arranged by size:
4090 1222 360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2669 3877 1353
Size: 1316 1208 3423
Fragments arranged by size:
3423 1316 1208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2836 4534 4908 5054 387
Size: 1064 138 1247 1698 374 146 1280
Fragments arranged by size:
1698 1280 1247 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2650 2770 4010 4607 5394 5662 1239
Size: 393 1018 120 1240 597 787 268 1524
Fragments arranged by size:
1524 1240 1018 787 597 393 268 120
EcoO109I rG'GnC_Cy
Cuts at: 2143 2143
Size: 5947
EcoRI G'AATT_C
Cuts at: 3256 3634 3256
Size: 378 5569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2326 2326
Size: 5947
FspI TGC'GCA
Cuts at: 4453 4453
Size: 5947
HaeII r_GCGC'y
Cuts at: 1678 2084 2112 2673 2918 5094 1678
Size: 406 28 561 245 2176 2531
Fragments arranged by size:
2531 2176 561 406 245 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4588 4620 4662 4694 1373
Size: 32 135 32 3016 32 42 32 2626
Fragments arranged by size:
3016 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2926 3291 3537 669
Size: 988 281 988 365 246 3079
Fragments arranged by size:
3079 988 988 365 281 246
HindIII A'AGCT_T
Cuts at: 748 3010 3089 748
Size: 2262 79 3606
Fragments arranged by size:
3606 2262 79
HpaI GTT'AAC
Cuts at: 3537 3537
Size: 5947
KasI G'GCGC_C
Cuts at: 2080 2108 2080
Size: 28 5919
KpnI G_GTAC'C
Cuts at: 3272 3272
Size: 5947
MfeI C'AATT_G
Cuts at: 3143 3546 3143
Size: 403 5544
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5061 5245 1305
Size: 3756 184 2007
Fragments arranged by size:
3756 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2181 10
Size: 54 1425 343 349 3776
Fragments arranged by size:
3776 1425 349 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3905 4264 4423 518
Size: 648 843 1896 359 159 2042
Fragments arranged by size:
2042 1896 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2943 3363 4040 5188 5433 1606
Size: 1337 420 677 1148 245 2120
Fragments arranged by size:
2120 1337 1148 677 420 245
NaeI GCC'GGC
Cuts at: 2824 2824
Size: 5947
NarI GG'CG_CC
Cuts at: 2081 2109 2081
Size: 28 5919
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4456
Fragments arranged by size:
4456 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5947
NgoMIV G'CCGG_C
Cuts at: 2822 2822
Size: 5947
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 3394 3394
Size: 5947
NsiI A_TGCA'T
Cuts at: 2606 2606
Size: 5947
NspI r_CATG'y
Cuts at: 1132 1229 2608 2834 3305 4850 1132
Size: 97 1379 226 471 1545 2229
Fragments arranged by size:
2229 1545 1379 471 226 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5947
PciI A'CATG_T
Cuts at: 4846 4846
Size: 5947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5947
PfoI T'CCnGG_A
Cuts at: 3192 3192
Size: 5947
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PmlI CAC'GTG
Cuts at: 2025 2289 2346 2025
Size: 264 57 5626
Fragments arranged by size:
5626 264 57
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 5529 5561 4016
Size: 32 1481 32 4402
Fragments arranged by size:
4402 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5947
PsiI TTA'TAA
Cuts at: 3517 3517
Size: 5947
PspOMI G'GGCC_C
Cuts at: 2143 2682 2143
Size: 539 5408
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2324 2356 2324
Size: 32 5915
PstI C_TGCA'G
Cuts at: 830 2715 3299 830
Size: 1885 584 3478
Fragments arranged by size:
3478 1885 584
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
PvuII CAG'CTG
Cuts at: 2943 2943
Size: 5947
SalI G'TCGA_C
Cuts at: 1936 3289 1936
Size: 1353 4594
SapI GCTCTTCn'nnn_
Cuts at: 2669 2669
Size: 5947
SbfI CC_TGCA'GG
Cuts at: 3299 3299
Size: 5947
ScaI AGT'ACT
Cuts at: 2402 4195 5907 2402
Size: 1793 1712 2442
Fragments arranged by size:
2442 1793 1712
SexAI A'CCwGG_T
Cuts at: 2724 2724
Size: 5947
SfcI C'TryA_G
Cuts at: 826 1045 2711 3295 4430 5111 5302 826
Size: 219 1666 584 1135 681 191 1471
Fragments arranged by size:
1666 1471 1135 681 584 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2142 2187 2196 2142
Size: 45 9 5893
Fragments arranged by size:
5893 45 9
SfoI GGC'GCC
Cuts at: 2082 2110 2082
Size: 28 5919
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5947
SmaI CCC'GGG
Cuts at: 1872 2202 3274 1872
Size: 330 1072 4545
Fragments arranged by size:
4545 1072 330
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2909 2948 4051 4952 5214 820
Size: 197 926 966 39 1103 901 262 1553
Fragments arranged by size:
1553 1103 966 926 901 262 197 39
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 2608 2834 3305 1132
Size: 1476 226 471 3774
Fragments arranged by size:
3774 1476 471 226
SspI AAT'ATT
Cuts at: 5 52 3871 5
Size: 47 3819 2081
Fragments arranged by size:
3819 2081 47
StuI AGG'CCT
Cuts at: 1517 2072 1517
Size: 555 5392
StyI C'CwwG_G
Cuts at: 513 1065 2004 2137 3386 513
Size: 552 939 133 1249 3074
Fragments arranged by size:
3074 1249 939 552 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3974 3991 4144 4329 4774 325
Size: 1255 2394 17 153 185 445 1498
Fragments arranged by size:
2394 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2400 2937 4193
Size: 274 80 33 51 1443 423 537 1256
Cuts at: 4193 5905 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1256 537 423 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5907 5939 5907
Size: 32 5915
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5947
XbaI T'CTAG_A
Cuts at: 3283 3283
Size: 5947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2189 2620 2189
Size: 431 5516
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 2200 3272 1870
Size: 330 1072 4545
Fragments arranged by size:
4545 1072 330
XmnI GAAnn'nnTTC
Cuts at: 4076 4076
Size: 5947
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3755 276
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI NruI PacI PpuMI PspXI RsrII SacI SacII
SanDI SrfI SwaI