Restriction map for
pFN21AB7695
(Circular) (Six-base) MAPSORT of: pFN21AB7695.seq Check: 7121 from: 1 to: 5866
pFN21AB7695 5866 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3676 278
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3209 1937
Size: 1272 4594
Acc65I G'GTAC_C
Cuts at: 3187 3187
Size: 5866
AclI AA'CG_TT
Cuts at: 1563 3993 4366 1563
Size: 2430 373 3063
Fragments arranged by size:
3063 2430 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2339 2420 3152 3929 5313 5581 1632
Size: 707 81 732 777 1384 268 1917
Fragments arranged by size:
1917 1384 777 732 707 268 81
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5669
AflIII A'CryG_T
Cuts at: 1203 2217 3057 4765 5742 1203
Size: 1014 840 1708 977 1327
Fragments arranged by size:
1708 1327 1014 977 840
AgeI A'CCGG_T
Cuts at: 4697 4697
Size: 5866
AhdI GACnn_n'nnGTC
Cuts at: 2859 4595 2859
Size: 1736 4130
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5866
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5866
AlwNI CAG_nnn'CTG
Cuts at: 2286 2443 3025 3609 5181 2286
Size: 157 582 584 1572 2971
Fragments arranged by size:
2971 1572 584 582 157
ApaLI G'TGCA_C
Cuts at: 3923 5079 3923
Size: 1156 4710
ApoI r'AATT_y
Cuts at: 1493 3175 3401 3553 5662 1493
Size: 1682 226 152 2109 1697
Fragments arranged by size:
2109 1697 1682 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4420 160
Size: 872 3388 1606
Fragments arranged by size:
3388 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
AvaI C'yCGr_G
Cuts at: 1870 1943 2203 2354 2462 2864 3191 1870
Size: 73 260 151 108 402 327 4545
Fragments arranged by size:
4545 402 327 260 151 108 73
AvrII C'CTAG_G
Cuts at: 2035 2035
Size: 5866
BamHI G'GATC_C
Cuts at: 3196 3563 3196
Size: 367 5499
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2791 3126 3187 4642 618
Size: 325 217 627 1004 335 61 1455 1842
Fragments arranged by size:
1842 1455 1004 627 335 325 217 61
BanII G_rGCy'C
Cuts at: 1391 2634 2846 3090 3185 1391
Size: 1243 212 244 95 4072
Fragments arranged by size:
4072 1243 244 212 95
BbeI G_GCGC'C
Cuts at: 2795 2795
Size: 5866
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2183 2478 2858 928
Size: 971 284 295 380 3936
Fragments arranged by size:
3936 971 380 295 284
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4057 4091 1903
Size: 34 2120 34 3678
Fragments arranged by size:
3678 2120 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3758 4968 3758
Size: 1210 4656
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5866
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2789 2841 4477
Size: 107 122 71 631 605 1117 52 1636
Cuts at: 4477 136
Size: 1525
Fragments arranged by size:
1636 1525 1117 631 605 122 107 71
52
BglII A'GATC_T
Cuts at: 5861 5861
Size: 5866
BlpI GC'TnA_GC
Cuts at: 2633 3283 2633
Size: 650 5216
Bme1580I G_kGCm'C
Cuts at: 1790 3927 5083 1790
Size: 2137 1156 2573
Fragments arranged by size:
2573 2137 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5866
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3597 4555 442
Size: 416 53 83 399 24 2180 958 1753
Fragments arranged by size:
2180 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 2465 2588 3124 4526 1239
Size: 1046 180 123 536 1402 2579
Fragments arranged by size:
2579 1402 1046 536 180 123
Bpu10I CC'TnA_GC
Cuts at: 2030 2030
Size: 5866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3991 4856 5154 3991
Size: 865 298 4703
Fragments arranged by size:
4703 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2405 2543 4529 882
Size: 617 906 138 1986 2219
Fragments arranged by size:
2219 1986 906 617 138
BsaAI yAC'GTr
Cuts at: 493 1204 2220 3657 493
Size: 711 1016 1437 2702
Fragments arranged by size:
2702 1437 1016 711
BsaBI GATnn'nnATC
Cuts at: 5860 5860
Size: 5866
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2792 3673 4055 4690
Size: 53 83 186 936 1259 881 382 635
Cuts at: 4690 275
Size: 1451
Fragments arranged by size:
1451 1259 936 881 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2541 2712 4299 4697 4971 5118 5529 2541
Size: 171 1587 398 274 147 411 2878
Fragments arranged by size:
2878 1587 411 398 274 171 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2861 2891 1178
Size: 30 165 30 1458 30 4153
Fragments arranged by size:
4153 1458 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 2423 2681 2684 2693 1186
Size: 1234 3 258 3 9 4359
Fragments arranged by size:
4359 1234 258 9 3 3
BseYI C'CCAG_C
Cuts at: 5069 5565 5069
Size: 496 5370
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2391 1163
Size: 573 655 4638
Fragments arranged by size:
4638 655 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2627 3046 3316 4077 5105 1540
Size: 275 186 626 419 270 761 1028 2301
Fragments arranged by size:
2301 1028 761 626 419 275 270 186
BsiHKAI G_wGCw'C
Cuts at: 2070 3927 4012 5083 2070
Size: 1857 85 1071 2853
Fragments arranged by size:
2853 1857 1071 85
BsmI GAATG_Cn'
Cuts at: 3377 3470 3377
Size: 93 5773
BsmBI CGTCTCn'nnnn_
Cuts at: 2369 2369
Size: 5866
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2634 2846 3090 3185 3927 4012
Size: 399 280 564 212 244 95 742 85
Cuts at: 4012 5083 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 564 399 280 244 212
95 85
BspEI T'CCGG_A
Cuts at: 2541 5529 2541
Size: 2988 2878
BspHI T'CATG_A
Cuts at: 3755 5485 3755
Size: 1730 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3221 844
Size: 2377 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3753 1394
Size: 429 1930 3507
Fragments arranged by size:
3507 1930 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4361 4535 65
Size: 1183 3113 174 1396
Fragments arranged by size:
3113 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2138 2347 2622 4510 4697 1700
Size: 110 328 209 275 1888 187 2869
Fragments arranged by size:
2869 1888 328 275 209 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5866
BssHII G'CGCG_C
Cuts at: 1925 2087 1925
Size: 162 5704
BssSI C'ACGA_G
Cuts at: 2911 3926 4938 2911
Size: 1015 1012 3839
Fragments arranged by size:
3839 1015 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2976 2985 2994 794
Size: 2182 9 9 3666
Fragments arranged by size:
3666 2182 9 9
BstBI TT'CG_AA
Cuts at: 4670 4670
Size: 5866
BstEII G'GTnAC_C
Cuts at: 1184 3050 1184
Size: 1866 4000
BstYI r'GATC_y
Cuts at: 1972 3196 3563 3948 3965 5417 5503 5515 5861
Size: 1224 367 385 17 1452 86 12 346
Cuts at: 5861 1972
Size: 1977
Fragments arranged by size:
1977 1452 1224 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2760 2760
Size: 5866
BtgI C'CryG_G
Cuts at: 513 1065 2004 2185 3078 513
Size: 552 939 181 893 3301
Fragments arranged by size:
3301 939 893 552 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5127
Fragments arranged by size:
5127 683 56
BtsI GCAGTG_nn'
Cuts at: 2068 2972 3382 4176 4196 2068
Size: 904 410 794 20 3738
Fragments arranged by size:
3738 904 794 410 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5831
DraI TTT'AAA
Cuts at: 3170 3170
Size: 5866
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2804 4873 809
Size: 507 1488 2069 1802
Fragments arranged by size:
2069 1802 1488 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2624 2661
Size: 54 1425 50 275 18 177 617 37
Cuts at: 2661 3313 4202 5831 8
Size: 652 889 1629 43
Fragments arranged by size:
1629 1425 889 652 617 275 177 54
50 43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 2624 3313 1537
Size: 275 812 689 4090
Fragments arranged by size:
4090 812 689 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3796 1353
Size: 2443 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3098 4453 4827 4973 387
Size: 1064 138 1509 1355 374 146 1280
Fragments arranged by size:
1509 1355 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2339 2420 2465 2588 3124 3152
Size: 393 653 54 81 45 123 536 28
Cuts at: 3152 3929 4526 5313 5581 1239
Size: 777 597 787 268 1524
Fragments arranged by size:
1524 787 777 653 597 536 393 268
123 81 54 45 28
EcoNI CCTnn'n_nnAGG
Cuts at: 2039 2321 2663 2039
Size: 282 342 5242
Fragments arranged by size:
5242 342 282
EcoO109I rG'GnC_Cy
Cuts at: 2269 2283 2755 2869 2269
Size: 14 472 114 5266
Fragments arranged by size:
5266 472 114 14
EcoRI G'AATT_C
Cuts at: 3175 3553 3175
Size: 378 5488
EcoRV GAT'ATC
Cuts at: 2599 2599
Size: 5866
FspI TGC'GCA
Cuts at: 4372 4372
Size: 5866
HaeII r_GCGC'y
Cuts at: 1678 2795 5013 1678
Size: 1117 2218 2531
Fragments arranged by size:
2531 2218 1117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2244 2276 2845 2861 2877
Size: 32 135 32 672 32 569 16 16
Cuts at: 2877 2893 2966 2998 4507 4539 4581 4613 1373
Size: 16 73 32 1509 32 42 32 2626
Fragments arranged by size:
2626 1509 672 569 135 73 42 32
32 32 32 32 32 16 16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3210 3456 669
Size: 988 281 1272 246 3079
Fragments arranged by size:
3079 1272 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5866
HpaI GTT'AAC
Cuts at: 3456 3456
Size: 5866
KasI G'GCGC_C
Cuts at: 2791 2791
Size: 5866
KpnI G_GTAC'C
Cuts at: 3191 3191
Size: 5866
MfeI C'AATT_G
Cuts at: 3465 3465
Size: 5866
MluI A'CGCG_T
Cuts at: 5742 5742
Size: 5866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2216 4980 5164 1305
Size: 911 2764 184 2007
Fragments arranged by size:
2764 2007 911 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3867
Fragments arranged by size:
3867 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 3824 4183 4342 518
Size: 648 1002 1656 359 159 2042
Fragments arranged by size:
2042 1656 1002 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2187 2232 2961 3022 3282 3959 5107 5352
Size: 581 45 729 61 260 677 1148 245
Cuts at: 5352 1606
Size: 2120
Fragments arranged by size:
2120 1148 729 677 581 260 245 61
45
NarI GG'CG_CC
Cuts at: 2792 2792
Size: 5866
NcoI C'CATG_G
Cuts at: 513 1065 2004 3078 513
Size: 552 939 1074 3301
Fragments arranged by size:
3301 1074 939 552
NdeI CA'TA_TG
Cuts at: 387 2524 387
Size: 2137 3729
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5866
NotI GC'GGCC_GC
Cuts at: 3313 3313
Size: 5866
NspI r_CATG'y
Cuts at: 1132 1229 3061 3224 4769 1132
Size: 97 1832 163 1545 2229
Fragments arranged by size:
2229 1832 1545 163 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5866
PciI A'CATG_T
Cuts at: 3057 4765 3057
Size: 1708 4158
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5866
PfoI T'CCnGG_A
Cuts at: 2262 2337 2262
Size: 75 5791
PmeI GTTT'AAAC
Cuts at: 3170 3170
Size: 5866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 5448 5480 3935
Size: 32 1481 32 4321
Fragments arranged by size:
4321 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2269 2755 2869 2269
Size: 486 114 5266
Fragments arranged by size:
5266 486 114
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5866
PsiI TTA'TAA
Cuts at: 3436 3436
Size: 5866
PspXI vC'TCGA_Gb
Cuts at: 2354 2462 2354
Size: 108 5758
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2144 2176 2144
Size: 32 5834
PstI C_TGCA'G
Cuts at: 830 2990 3218 830
Size: 2160 228 3478
Fragments arranged by size:
3478 2160 228
PvuI CG_AT'CG
Cuts at: 2001 3046 2001
Size: 1045 4821
PvuII CAG'CTG
Cuts at: 3022 3022
Size: 5866
SacII CC_GC'GG
Cuts at: 2188 2188
Size: 5866
SalI G'TCGA_C
Cuts at: 1936 3208 1936
Size: 1272 4594
SanDI GG'GwC_CC
Cuts at: 2755 2755
Size: 5866
SbfI CC_TGCA'GG
Cuts at: 3218 3218
Size: 5866
ScaI AGT'ACT
Cuts at: 4114 5826 4114
Size: 1712 4154
SfcI C'TryA_G
Cuts at: 826 1045 2986 3214 4349 5030 5221 826
Size: 219 1941 228 1135 681 191 1471
Fragments arranged by size:
1941 1471 1135 681 228 219 191
SfoI GGC'GCC
Cuts at: 2793 2793
Size: 5866
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5866
SmaI CCC'GGG
Cuts at: 1872 2205 3193 1872
Size: 333 988 4545
Fragments arranged by size:
4545 988 333
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2354 2462 3970 4871 5133 820
Size: 197 926 411 108 1508 901 262 1553
Fragments arranged by size:
1553 1508 926 901 411 262 197 108
SnaBI TAC'GTA
Cuts at: 493 3657 493
Size: 3164 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5866
SphI G_CATG'C
Cuts at: 1132 3224 1132
Size: 2092 3774
SspI AAT'ATT
Cuts at: 5 52 3790 5
Size: 47 3738 2081
Fragments arranged by size:
3738 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5866
StyI C'CwwG_G
Cuts at: 513 1065 2004 2035 3078 3305 513
Size: 552 939 31 1043 227 3074
Fragments arranged by size:
3074 1043 939 552 227 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2589 2663 2669 3893 3910 4063 4248
Size: 1255 1009 74 6 1224 17 153 185
Cuts at: 4248 4693 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1224 1009 445 185 153 74
17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2292 3060 4112
Size: 274 80 33 51 1443 315 768 1052
Cuts at: 4112 5824 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1052 768 315 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5826 5858 5826
Size: 32 5834
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5866
XbaI T'CTAG_A
Cuts at: 3202 3202
Size: 5866
XhoI C'TCGA_G
Cuts at: 1943 2354 2462 1943
Size: 411 108 5347
Fragments arranged by size:
5347 411 108
XmaI C'CCGG_G
Cuts at: 1870 2203 3191 1870
Size: 333 988 4545
Fragments arranged by size:
4545 988 333
XmnI GAAnn'nnTTC
Cuts at: 3995 3995
Size: 5866
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3674 276
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI
BplI BsiWI BstXI BstZ17I DraIII EcoICRI FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI
RsrII SacI SapI SexAI SfiI SrfI SwaI XcmI