Restriction map for
pFN21AB7693
(Circular) (Six-base) MAPSORT of: pFN21AB7693.seq Check: 1625 from: 1 to: 5755
pFN21AB7693 5755 bp
With 182 enzymes: SgfI *
March 28, 2012 12:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2176 2777 2912 3818 5202 5470 1632
Size: 544 601 135 906 1384 268 1917
Fragments arranged by size:
1917 1384 906 601 544 268 135
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 4654 5631 1203
Size: 3451 977 1327
Fragments arranged by size:
3451 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AlwNI CAG_nnn'CTG
Cuts at: 2459 3498 5070 2459
Size: 1039 1572 3144
Fragments arranged by size:
3144 1572 1039
ApaI G_GGCC'C
Cuts at: 2965 2965
Size: 5755
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 3064 3290 3442 5551 1493
Size: 1571 226 152 2109 1697
Fragments arranged by size:
2109 1697 1571 226 152
AscI GG'CGCG_CC
Cuts at: 2334 2334
Size: 5755
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 2314 2371 3080 1870
Size: 73 371 57 709 4545
Fragments arranged by size:
4545 709 371 73 57
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2023 3040 3076 4531 618
Size: 325 217 627 236 1017 36 1455 1842
Fragments arranged by size:
1842 1455 1017 627 325 236 217 36
BanII G_rGCy'C
Cuts at: 1391 2772 2965 3007 3074 1391
Size: 1381 193 42 67 4072
Fragments arranged by size:
4072 1381 193 67 42
BbeI G_GCGC'C
Cuts at: 2027 3044 2027
Size: 1017 4738
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2648 928
Size: 971 749 4035
Fragments arranged by size:
4035 971 749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2074 2108 3946 3980 1903
Size: 34 137 34 1838 34 3678
Fragments arranged by size:
3678 1838 137 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 2896 1548
Size: 1348 4407
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2267 2279 2354
Size: 107 122 71 631 605 595 12 75
Cuts at: 2354 4366 136
Size: 2012 1525
Fragments arranged by size:
2012 1525 631 605 595 122 107 75
71 12
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 2249 3172 2249
Size: 923 4832
Bme1580I G_kGCm'C
Cuts at: 1790 2965 3816 4972 1790
Size: 1175 851 1156 2573
Fragments arranged by size:
2573 1175 1156 851
BmgBI CAC'GTC
Cuts at: 1318 2438 1318
Size: 1120 4635
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3045 3486 4444
Size: 416 53 83 399 24 1628 441 958
Cuts at: 4444 442
Size: 1753
Fragments arranged by size:
1753 1628 958 441 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2534 4415 1239
Size: 1295 1881 2579
Fragments arranged by size:
2579 1881 1295
Bpu10I CC'TnA_GC
Cuts at: 2702 2702
Size: 5755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2405 3880 4745 5043 2405
Size: 1475 865 298 3117
Fragments arranged by size:
3117 1475 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4418 882
Size: 617 2919 2219
Fragments arranged by size:
2919 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2024 3041 3562 3944
Size: 53 83 186 936 491 1017 521 382
Cuts at: 3944 4579 275
Size: 635 1451
Fragments arranged by size:
1451 1017 936 635 521 491 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2663 4188 4586 4860 5007 5418 2663
Size: 1525 398 274 147 411 3000
Fragments arranged by size:
3000 1525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5530
Fragments arranged by size:
5530 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2402 2780 1186
Size: 1216 378 4161
Fragments arranged by size:
4161 1216 378
BseYI C'CCAG_C
Cuts at: 2220 2964 4958 5454 2220
Size: 744 1994 496 2521
Fragments arranged by size:
2521 1994 744 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2283 2820 3205 3966 4994 1540
Size: 275 186 282 537 385 761 1028 2301
Fragments arranged by size:
2301 1028 761 537 385 282 275 186
BsiHKAI G_wGCw'C
Cuts at: 2313 3816 3901 4972 2313
Size: 1503 85 1071 3096
Fragments arranged by size:
3096 1503 1071 85
BsmI GAATG_Cn'
Cuts at: 2488 3266 3359 2488
Size: 778 93 4884
Fragments arranged by size:
4884 778 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2313 2772 2965 3007 3074 3816 3901
Size: 399 523 459 193 42 67 742 85
Cuts at: 3901 4972 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 523 459 399 193 85
67 42
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3110 844
Size: 2266 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2170 3642 1394
Size: 429 347 1472 3507
Fragments arranged by size:
3507 1472 429 347
BsrDI GCAATG_nn'
Cuts at: 65 1248 4250 4424 65
Size: 1183 3002 174 1396
Fragments arranged by size:
3002 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2970 4399 4586 1700
Size: 110 1160 1429 187 2869
Fragments arranged by size:
2869 1429 1160 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 2334 1925
Size: 409 5346
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5755
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 3085 3452 3837 3854 5306 5392 5404 5750
Size: 1113 367 385 17 1452 86 12 346
Cuts at: 5750 1972
Size: 1977
Fragments arranged by size:
1977 1452 1113 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2028 2213 2262 2534 2772 2814 513
Size: 552 963 185 49 272 238 42 3454
Fragments arranged by size:
3454 963 552 272 238 185 49 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5016
Fragments arranged by size:
5016 683 56
BtsI GCAGTG_nn'
Cuts at: 3271 4065 4085 3271
Size: 794 20 4941
Fragments arranged by size:
4941 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DraIII CAC_nnn'GTG
Cuts at: 2242 2242
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2280 2326 2413
Size: 54 1425 50 275 18 450 46 87
Cuts at: 2413 2817 3202 4091 5720 8
Size: 404 385 889 1629 43
Fragments arranged by size:
1629 1425 889 450 404 385 275 87
54 50 46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2280 2817 3202 1537
Size: 275 468 537 385 4090
Fragments arranged by size:
4090 537 468 385 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2753 2934 2948 3685 1353
Size: 1400 181 14 737 3423
Fragments arranged by size:
3423 1400 737 181 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2048 2350 4342 4716 4862 387
Size: 1064 138 459 302 1992 374 146 1280
Fragments arranged by size:
1992 1280 1064 459 374 302 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 2534 2777 2912 3818 4415 5202
Size: 393 544 358 243 135 906 597 787
Cuts at: 5202 5470 1239
Size: 268 1524
Fragments arranged by size:
1524 906 787 597 544 393 358 268
243 135
EcoNI CCTnn'n_nnAGG
Cuts at: 2749 2749
Size: 5755
EcoO109I rG'GnC_Cy
Cuts at: 2583 2911 2961 2583
Size: 328 50 5377
Fragments arranged by size:
5377 328 50
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 2027 2221 2971 2995 3044 4902 1678
Size: 349 194 750 24 49 1858 2531
Fragments arranged by size:
2531 1858 750 349 194 49 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2477 2509 2572 2604 4396
Size: 32 135 32 905 32 63 32 1792
Cuts at: 4396 4428 4470 4502 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1792 905 135 63 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3099 3345 669
Size: 988 281 1161 246 3079
Fragments arranged by size:
3079 1161 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KasI G'GCGC_C
Cuts at: 2023 3040 2023
Size: 1017 4738
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2458 3073 4869 5053 1305
Size: 1153 615 1796 184 2007
Fragments arranged by size:
2007 1796 1153 615 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3933
Fragments arranged by size:
3933 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2574 3713 4072 4231 518
Size: 648 1408 1139 359 159 2042
Fragments arranged by size:
2042 1408 1139 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2264 2412 2816 2986 3171 3848 4996 5241
Size: 658 148 404 170 185 677 1148 245
Cuts at: 5241 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 658 404 245 185 170
148
NaeI GCC'GGC
Cuts at: 2972 2972
Size: 5755
NarI GG'CG_CC
Cuts at: 2024 3041 2024
Size: 1017 4738
NcoI C'CATG_G
Cuts at: 513 1065 2028 513
Size: 552 963 4240
Fragments arranged by size:
4240 963 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NgoMIV G'CCGG_C
Cuts at: 2970 2970
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 3113 4658 1132
Size: 97 1884 1545 2229
Fragments arranged by size:
2229 1884 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2255 1348
Size: 907 4848
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2609 1108
Size: 1501 4254
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2911 2911
Size: 5755
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PspOMI G'GGCC_C
Cuts at: 2961 2961
Size: 5755
PstI C_TGCA'G
Cuts at: 830 2296 2979 3107 830
Size: 1466 683 128 3478
Fragments arranged by size:
3478 1466 683 128
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
SacII CC_GC'GG
Cuts at: 2265 2817 2265
Size: 552 5203
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 2292 2975 3103 4238 4919 5110 826
Size: 219 1247 683 128 1135 681 191 1471
Fragments arranged by size:
1471 1247 1135 683 681 219 191 128
SfiI GGCCn_nnn'nGGCC
Cuts at: 2267 2267
Size: 5755
SfoI GGC'GCC
Cuts at: 2025 3042 2025
Size: 1017 4738
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2420 3859 4760 5022 820
Size: 197 926 477 1439 901 262 1553
Fragments arranged by size:
1553 1439 926 901 477 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 2028 3194 513
Size: 552 963 1166 3074
Fragments arranged by size:
3074 1166 963 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2092 2729 3782 3799 3952 4137 4582
Size: 1255 512 637 1053 17 153 185 445
Cuts at: 4582 325
Size: 1498
Fragments arranged by size:
1498 1255 1053 637 512 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4001 5713 96
Size: 274 80 33 51 1443 2024 1712 138
Fragments arranged by size:
2024 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5715 5747 5715
Size: 32 5723
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2423 2423
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 2925 3884 2925
Size: 959 4796
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SrfI
SwaI