Restriction map for
pFN21AB7690
(Circular) (Six-base) MAPSORT of: pFN21AB7690.seq Check: 8460 from: 1 to: 5938
pFN21AB7690 5938 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3748 278
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2476 3089 3126 3281 1937
Size: 539 613 37 155 4594
Fragments arranged by size:
4594 613 539 155 37
Acc65I G'GTAC_C
Cuts at: 3259 3259
Size: 5938
AclI AA'CG_TT
Cuts at: 1563 4065 4438 1563
Size: 2502 373 3063
Fragments arranged by size:
3063 2502 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2553 4001 5385 5653 1632
Size: 921 1448 1384 268 1917
Fragments arranged by size:
1917 1448 1384 921 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5741
AflIII A'CryG_T
Cuts at: 1203 4837 5814 1203
Size: 3634 977 1327
Fragments arranged by size:
3634 1327 977
AgeI A'CCGG_T
Cuts at: 4769 4769
Size: 5938
AhdI GACnn_n'nnGTC
Cuts at: 2650 4667 2650
Size: 2017 3921
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5938
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5938
AlwNI CAG_nnn'CTG
Cuts at: 2048 2456 3681 5253 2048
Size: 408 1225 1572 2733
Fragments arranged by size:
2733 1572 1225 408
ApaLI G'TGCA_C
Cuts at: 3995 5151 3995
Size: 1156 4782
ApoI r'AATT_y
Cuts at: 1493 2619 2777 3247 3473 3625 5734 1493
Size: 1126 158 470 226 152 2109 1697
Fragments arranged by size:
2109 1697 1126 470 226 158 152
AseI AT'TA_AT
Cuts at: 160 1032 4492 160
Size: 872 3460 1606
Fragments arranged by size:
3460 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
AvaI C'yCGr_G
Cuts at: 1870 1943 2137 3263 1870
Size: 73 194 1126 4545
Fragments arranged by size:
4545 1126 194 73
BamHI G'GATC_C
Cuts at: 3268 3635 3268
Size: 367 5571
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2638 3259 4714 618
Size: 325 217 627 851 621 1455 1842
Fragments arranged by size:
1842 1455 851 627 621 325 217
BanII G_rGCy'C
Cuts at: 1391 3257 1391
Size: 1866 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4967
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4129 4163 1903
Size: 34 2192 34 3678
Fragments arranged by size:
3678 2192 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2078 3830 5040 2078
Size: 1752 1210 2976
Fragments arranged by size:
2976 1752 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5938
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4549 136
Size: 107 122 71 631 605 2877 1525
Fragments arranged by size:
2877 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2320 5933 2320
Size: 3613 2325
BlpI GC'TnA_GC
Cuts at: 2937 3355 2937
Size: 418 5520
Bme1580I G_kGCm'C
Cuts at: 1790 3999 5155 1790
Size: 2209 1156 2573
Fragments arranged by size:
2573 2209 1156
BmgBI CAC'GTC
Cuts at: 1318 3087 1318
Size: 1769 4169
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3669 4627 442
Size: 416 53 83 399 24 2252 958 1753
Fragments arranged by size:
2252 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5938
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2505 2505
Size: 5938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4598 1239
Size: 3359 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4063 4928 5226 4063
Size: 865 298 4775
Fragments arranged by size:
4775 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4601 882
Size: 617 3102 2219
Fragments arranged by size:
3102 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3729 493
Size: 711 2525 2702
Fragments arranged by size:
2702 2525 711
BsaBI GATnn'nnATC
Cuts at: 5932 5932
Size: 5938
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3745 4127 4762 275
Size: 53 83 186 936 2212 382 635 1451
Fragments arranged by size:
2212 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4371 4769 5043 5190 5601 4371
Size: 398 274 147 411 4708
Fragments arranged by size:
4708 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2029 2059 3076 3106 1178
Size: 30 165 30 626 30 1017 30 4010
Fragments arranged by size:
4010 1017 626 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3008 3047 3209 1186
Size: 1822 39 162 3915
Fragments arranged by size:
3915 1822 162 39
BseYI C'CCAG_C
Cuts at: 2041 2063 2125 5141 5637 2041
Size: 22 62 3016 496 2342
Fragments arranged by size:
3016 2342 496 62 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2469 1163
Size: 573 733 4632
Fragments arranged by size:
4632 733 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3388 4149 5177 1540
Size: 275 186 1387 761 1028 2301
Fragments arranged by size:
2301 1387 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2144 3999 4084 5155 2144
Size: 1855 85 1071 2927
Fragments arranged by size:
2927 1855 1071 85
BsmI GAATG_Cn'
Cuts at: 2520 2679 3449 3542 2520
Size: 159 770 93 4916
Fragments arranged by size:
4916 770 159 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 3257 3999 4084 5155 1391
Size: 399 354 1113 742 85 1071 2174
Fragments arranged by size:
2174 1113 1071 742 399 354 85
BspEI T'CCGG_A
Cuts at: 5601 5601
Size: 5938
BspHI T'CATG_A
Cuts at: 3827 5557 3827
Size: 1730 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2668 2683 3293 844
Size: 1824 15 610 3489
Fragments arranged by size:
3489 1824 610 15
BsrBI CCG'CTC
Cuts at: 1394 1823 3825 1394
Size: 429 2002 3507
Fragments arranged by size:
3507 2002 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4433 4607 65
Size: 1183 3185 174 1396
Fragments arranged by size:
3185 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4582 4769 1700
Size: 110 2772 187 2869
Fragments arranged by size:
2869 2772 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5938
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5938
BssSI C'ACGA_G
Cuts at: 3998 5010 3998
Size: 1012 4926
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2456 794
Size: 1662 4276
BstBI TT'CG_AA
Cuts at: 4742 4742
Size: 5938
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5938
BstXI CCAn_nnnn'nTGG
Cuts at: 3071 3071
Size: 5938
BstYI r'GATC_y
Cuts at: 1972 2320 2536 3208 3268 3635 4020 4037 5489
Size: 348 216 672 60 367 385 17 1452
Cuts at: 5489 5575 5587 5933 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 672 385 367 348 346 216
86 60 17 12
Bsu36I CC'TnA_GG
Cuts at: 2964 3096 2964
Size: 132 5806
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5386
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5199
Fragments arranged by size:
5199 683 56
BtsI GCAGTG_nn'
Cuts at: 3454 4248 4268 3454
Size: 794 20 5124
Fragments arranged by size:
5124 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3091 3126 529
Size: 35 2527 35 3341
Fragments arranged by size:
3341 2527 35 35
DraI TTT'AAA
Cuts at: 2352 3242 2352
Size: 890 5048
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4945 809
Size: 507 3629 1802
Fragments arranged by size:
3629 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3385 4274 5903
Size: 54 1425 50 275 18 1555 889 1629
Cuts at: 5903 8
Size: 43
Fragments arranged by size:
1629 1555 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3385 1537
Size: 275 1573 4090
Fragments arranged by size:
4090 1573 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2765 3868 1353
Size: 1412 1103 3423
Fragments arranged by size:
3423 1412 1103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4525 4899 5045 387
Size: 1064 138 2936 374 146 1280
Fragments arranged by size:
2936 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2553 4001 4598 5385 5653 1239
Size: 393 921 1448 597 787 268 1524
Fragments arranged by size:
1524 1448 921 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2224 2224
Size: 5938
EcoO109I rG'GnC_Cy
Cuts at: 2680 2680
Size: 5938
EcoRI G'AATT_C
Cuts at: 3247 3625 3247
Size: 378 5560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2037 2753 2037
Size: 716 5222
FspI TGC'GCA
Cuts at: 4444 4444
Size: 5938
HaeII r_GCGC'y
Cuts at: 1678 5085 1678
Size: 3407 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2636 2668 4579 4611 4653
Size: 32 135 32 1064 32 1911 32 42
Cuts at: 4653 4685 1373
Size: 32 2626
Fragments arranged by size:
2626 1911 1064 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2477 3127 3282 3528 669
Size: 988 281 539 650 155 246 3079
Fragments arranged by size:
3079 988 650 539 281 246 155
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5938
HpaI GTT'AAC
Cuts at: 3528 3528
Size: 5938
KpnI G_GTAC'C
Cuts at: 3263 3263
Size: 5938
MfeI C'AATT_G
Cuts at: 3537 3537
Size: 5938
MluI A'CGCG_T
Cuts at: 5814 5814
Size: 5938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2289 5052 5236 1305
Size: 984 2763 184 2007
Fragments arranged by size:
2763 2007 984 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4116
Fragments arranged by size:
4116 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3020 3896 4255 4414 518
Size: 648 1854 876 359 159 2042
Fragments arranged by size:
2042 1854 876 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2212 2864 3354 4031 5179 5424 1606
Size: 606 652 490 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 652 606 490 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5386
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5938
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5938
NotI GC'GGCC_GC
Cuts at: 3385 3385
Size: 5938
NspI r_CATG'y
Cuts at: 1132 1229 2314 3296 4841 1132
Size: 97 1085 982 1545 2229
Fragments arranged by size:
2229 1545 1085 982 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5938
PciI A'CATG_T
Cuts at: 4837 4837
Size: 5938
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5938
PfoI T'CCnGG_A
Cuts at: 2781 2781
Size: 5938
PmeI GTTT'AAAC
Cuts at: 3242 3242
Size: 5938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4007 4039 5520 5552 4007
Size: 32 1481 32 4393
Fragments arranged by size:
4393 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2680 2680
Size: 5938
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5938
PsiI TTA'TAA
Cuts at: 3508 3508
Size: 5938
PstI C_TGCA'G
Cuts at: 830 3290 830
Size: 2460 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5938
PvuII CAG'CTG
Cuts at: 2864 2864
Size: 5938
SalI G'TCGA_C
Cuts at: 1936 2475 3125 3280 1936
Size: 539 650 155 4594
Fragments arranged by size:
4594 650 539 155
SbfI CC_TGCA'GG
Cuts at: 3290 3290
Size: 5938
ScaI AGT'ACT
Cuts at: 4186 5898 4186
Size: 1712 4226
SfcI C'TryA_G
Cuts at: 826 1045 2119 3286 4421 5102 5293 826
Size: 219 1074 1167 1135 681 191 1471
Fragments arranged by size:
1471 1167 1135 1074 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5938
SmaI CCC'GGG
Cuts at: 1872 3265 1872
Size: 1393 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4042 4943 5205 820
Size: 197 926 2099 901 262 1553
Fragments arranged by size:
2099 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3729 493
Size: 3236 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5938
SphI G_CATG'C
Cuts at: 1132 3296 1132
Size: 2164 3774
SspI AAT'ATT
Cuts at: 5 52 2348 3862 5
Size: 47 2296 1514 2081
Fragments arranged by size:
2296 2081 1514 47
StuI AGG'CCT
Cuts at: 1517 2399 1517
Size: 882 5056
StyI C'CwwG_G
Cuts at: 513 1065 3377 513
Size: 552 2312 3074
Fragments arranged by size:
3074 2312 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2640 3965 3982 4135 4320 4765 325
Size: 1255 1060 1325 17 153 185 445 1498
Fragments arranged by size:
1498 1325 1255 1060 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 4184 5896
Size: 274 80 33 51 1443 402 1805 1712
Cuts at: 5896 96
Size: 138
Fragments arranged by size:
1805 1712 1443 402 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2029 2061 3076 3108 5898 5930 2029
Size: 32 1015 32 2790 32 2037
Fragments arranged by size:
2790 2037 1015 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5938
XbaI T'CTAG_A
Cuts at: 3274 3274
Size: 5938
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5938
XmaI C'CCGG_G
Cuts at: 1870 3263 1870
Size: 1393 4545
XmnI GAAnn'nnTTC
Cuts at: 2739 4067 2739
Size: 1328 4610
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3746 276
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI