Restriction map for
pFN21AB7689
(Circular) (Six-base) MAPSORT of: pFN21AB7689.seq Check: 3380 from: 1 to: 5752
pFN21AB7689 5752 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2328 3095 1937
Size: 391 767 4594
Fragments arranged by size:
4594 767 391
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2305 3815 5199 5467 1632
Size: 673 1510 1384 268 1917
Fragments arranged by size:
1917 1510 1384 673 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 4651 5628 1203
Size: 3448 977 1327
Fragments arranged by size:
3448 1327 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2915 1184
Size: 1731 4021
AlwNI CAG_nnn'CTG
Cuts at: 2916 3495 5067 2916
Size: 579 1572 3601
Fragments arranged by size:
3601 1572 579
ApaLI G'TGCA_C
Cuts at: 3809 4965 3809
Size: 1156 4596
ApoI r'AATT_y
Cuts at: 1493 2242 2260 3061 3287 3439 5548 1493
Size: 749 18 801 226 152 2109 1697
Fragments arranged by size:
2109 1697 801 749 226 152 18
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 2068 2808 2994 3077 1870
Size: 73 125 740 186 83 4545
Fragments arranged by size:
4545 740 186 125 83 73
BamHI G'GATC_C
Cuts at: 3082 3449 3082
Size: 367 5385
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2899 3073 4528 618
Size: 325 217 627 1112 174 1455 1842
Fragments arranged by size:
1842 1455 1112 627 325 217 174
BanII G_rGCy'C
Cuts at: 1391 2076 2661 3071 1391
Size: 685 585 410 4072
Fragments arranged by size:
4072 685 585 410
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2400 2679 928
Size: 971 501 279 4001
Fragments arranged by size:
4001 971 501 279
BbvCI CC'TCA_GC
Cuts at: 2792 2792
Size: 5752
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3943 3977 1903
Size: 34 50 34 1922 34 3678
Fragments arranged by size:
3678 1922 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3644 4854 3644
Size: 1210 4542
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5752
BfrBI ATG'CAT
Cuts at: 2122 2122
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2758 4363 136
Size: 107 122 71 631 605 1086 1605 1525
Fragments arranged by size:
1605 1525 1086 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2673 5747 2673
Size: 3074 2678
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 2724 3813 4969 1790
Size: 934 1089 1156 2573
Fragments arranged by size:
2573 1156 1089 934
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2185 3483 4441
Size: 416 53 83 399 24 768 1298 958
Cuts at: 4441 442
Size: 1753
Fragments arranged by size:
1753 1298 958 768 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2852 4412 1239
Size: 821 792 1560 2579
Fragments arranged by size:
2579 1560 821 792
Bpu10I CC'TnA_GC
Cuts at: 2792 2792
Size: 5752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2060 3877 4742 5040 2060
Size: 1817 865 298 2772
Fragments arranged by size:
2772 1817 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2984 4415 882
Size: 617 1485 1431 2219
Fragments arranged by size:
2219 1485 1431 617
BsaAI yAC'GTr
Cuts at: 493 1204 3543 493
Size: 711 2339 2702
Fragments arranged by size:
2702 2339 711
BsaBI GATnn'nnATC
Cuts at: 5746 5746
Size: 5752
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2837 3559 3941 4576
Size: 53 83 186 936 1304 722 382 635
Cuts at: 4576 275
Size: 1451
Fragments arranged by size:
1451 1304 936 722 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2860 2896 4185 4583 4857 5004 5415 2860
Size: 36 1289 398 274 147 411 3197
Fragments arranged by size:
3197 1289 411 398 274 147 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2850 2880 2897 2927 1178
Size: 30 165 30 1447 30 17 30 4003
Fragments arranged by size:
4003 1447 165 30 30 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2900 1186
Size: 871 843 4038
Fragments arranged by size:
4038 871 843
BseYI C'CCAG_C
Cuts at: 2723 2959 4955 5451 2723
Size: 236 1996 496 3024
Fragments arranged by size:
3024 1996 496 236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5179
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3963 4991 1540
Size: 275 186 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2661 3813 3898 4969 2661
Size: 1152 85 1071 3444
Fragments arranged by size:
3444 1152 1071 85
BsmI GAATG_Cn'
Cuts at: 2340 3263 3356 2340
Size: 923 93 4736
Fragments arranged by size:
4736 923 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2661 2724 3071 3813 3898 4969
Size: 399 286 585 63 347 742 85 1071
Cuts at: 4969 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 585 399 347 286 85
63
BspEI T'CCGG_A
Cuts at: 5415 5415
Size: 5752
BspHI T'CATG_A
Cuts at: 3641 5371 3641
Size: 1730 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2829 3107 844
Size: 1985 278 3489
Fragments arranged by size:
3489 1985 278
BsrBI CCG'CTC
Cuts at: 1394 1823 3639 1394
Size: 429 1816 3507
Fragments arranged by size:
3507 1816 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4247 4421 65
Size: 1183 2999 174 1396
Fragments arranged by size:
2999 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 2641 4396 4583 1700
Size: 110 198 633 1755 187 2869
Fragments arranged by size:
2869 1755 633 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5752
BstBI TT'CG_AA
Cuts at: 4556 4556
Size: 5752
BstEII G'GTnAC_C
Cuts at: 1184 2617 2872 1184
Size: 1433 255 4064
Fragments arranged by size:
4064 1433 255
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5752
BstYI r'GATC_y
Cuts at: 1972 2109 2673 3082 3449 3834 3851 5303 5389
Size: 137 564 409 367 385 17 1452 86
Cuts at: 5389 5401 5747 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 564 409 385 367 346 137
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2033 513
Size: 552 968 4232
Fragments arranged by size:
4232 968 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2742 487
Size: 683 56 1516 3497
Fragments arranged by size:
3497 1516 683 56
BtsI GCAGTG_nn'
Cuts at: 3268 4062 4082 3268
Size: 794 20 4938
Fragments arranged by size:
4938 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2870 4759 809
Size: 507 1554 1889 1802
Fragments arranged by size:
1889 1802 1554 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2750 2978 3199
Size: 54 1425 50 275 18 920 228 221
Cuts at: 3199 4088 5717 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 920 889 275 228 221 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2727 2759 3682 1353
Size: 1374 32 923 3423
Fragments arranged by size:
3423 1374 923 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2580 2776 4339 4713 4859 387
Size: 1064 138 991 196 1563 374 146 1280
Fragments arranged by size:
1563 1280 1064 991 374 196 146 138
EcoICRI GAG'CTC
Cuts at: 2659 2659
Size: 5752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2305 2852 3815 4412 5199 5467
Size: 393 428 245 547 963 597 787 268
Cuts at: 5467 1239
Size: 1524
Fragments arranged by size:
1524 963 787 597 547 428 393 268
245
EcoRI G'AATT_C
Cuts at: 2260 3061 3439 2260
Size: 801 378 4573
Fragments arranged by size:
4573 801 378
EcoRV GAT'ATC
Cuts at: 3025 3025
Size: 5752
FspI TGC'GCA
Cuts at: 2024 4258 2024
Size: 2234 3518
FspAI rTGC'GCAy
Cuts at: 2024 2024
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 4899 1678
Size: 3221 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4393 4425 4467 4499 1373
Size: 32 135 32 2821 32 42 32 2626
Fragments arranged by size:
2821 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3342 669
Size: 988 281 1158 246 3079
Fragments arranged by size:
3079 1158 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5752
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 3351 3351
Size: 5752
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2082 2877 4866 5050 1305
Size: 777 795 1989 184 2007
Fragments arranged by size:
2007 1989 795 777 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3930
Fragments arranged by size:
3930 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3710 4069 4228 518
Size: 648 843 1701 359 159 2042
Fragments arranged by size:
2042 1701 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3168 3845 4993 5238 1606
Size: 1562 677 1148 245 2120
Fragments arranged by size:
2120 1562 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5200
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NsiI A_TGCA'T
Cuts at: 2124 2124
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 2029 2126 3110 4655 1132
Size: 97 800 97 984 1545 2229
Fragments arranged by size:
2229 1545 984 800 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5752
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2232 1108
Size: 1124 4628
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5752
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PspXI vC'TCGA_Gb
Cuts at: 2808 2808
Size: 5752
PstI C_TGCA'G
Cuts at: 830 2673 3104 830
Size: 1843 431 3478
Fragments arranged by size:
3478 1843 431
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
SacI G_AGCT'C
Cuts at: 2661 2661
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SfcI C'TryA_G
Cuts at: 826 1045 2669 3100 4235 4916 5107 826
Size: 219 1624 431 1135 681 191 1471
Fragments arranged by size:
1624 1471 1135 681 431 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 2070 2996 3079 1872
Size: 198 926 83 4545
Fragments arranged by size:
4545 926 198 83
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2075 2808 3856 4757 5019 820
Size: 197 926 132 733 1048 901 262 1553
Fragments arranged by size:
1553 1048 926 901 733 262 197 132
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 2029 2126 3110 1132
Size: 897 97 984 3774
Fragments arranged by size:
3774 984 897 97
SspI AAT'ATT
Cuts at: 5 52 2773 3676 5
Size: 47 2721 903 2081
Fragments arranged by size:
2721 2081 903 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5752
StyI C'CwwG_G
Cuts at: 513 1065 3191 513
Size: 552 2126 3074
Fragments arranged by size:
3074 2126 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2127 3044 3779 3796 3949 4134 4579
Size: 1255 547 917 735 17 153 185 445
Cuts at: 4579 325
Size: 1498
Fragments arranged by size:
1498 1255 917 735 547 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3998 5710 96
Size: 274 80 33 51 1443 2021 1712 138
Fragments arranged by size:
2021 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5712 5744 5712
Size: 32 5720
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XhoI C'TCGA_G
Cuts at: 1943 2808 1943
Size: 865 4887
XmaI C'CCGG_G
Cuts at: 1870 2068 2994 3077 1870
Size: 198 926 83 4545
Fragments arranged by size:
4545 926 198 83
XmnI GAAnn'nnTTC
Cuts at: 3881 3881
Size: 5752
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
FalI FseI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI