Restriction map for
pFN21AB7688
(Circular) (Six-base) MAPSORT of: pFN21AB7688.seq Check: 6002 from: 1 to: 5935
pFN21AB7688 5935 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2787 2787
Size: 5935
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3745 278
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2511 3278 1937
Size: 574 767 4594
Fragments arranged by size:
4594 767 574
Acc65I G'GTAC_C
Cuts at: 3256 3256
Size: 5935
AclI AA'CG_TT
Cuts at: 1563 4062 4435 1563
Size: 2499 373 3063
Fragments arranged by size:
3063 2499 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2924 3998 5382 5650 1632
Size: 1292 1074 1384 268 1917
Fragments arranged by size:
1917 1384 1292 1074 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5738
AflIII A'CryG_T
Cuts at: 1203 4834 5811 1203
Size: 3631 977 1327
Fragments arranged by size:
3631 1327 977
AgeI A'CCGG_T
Cuts at: 4766 4766
Size: 5935
AhdI GACnn_n'nnGTC
Cuts at: 2507 4664 2507
Size: 2157 3778
AleI CACnn'nnGTG
Cuts at: 1166 2231 1166
Size: 1065 4870
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5935
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2270 2302 2534 2566 2270
Size: 32 232 32 5639
Fragments arranged by size:
5639 232 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3678 5250 3678
Size: 1572 4363
ApaLI G'TGCA_C
Cuts at: 3992 5148 3992
Size: 1156 4779
ApoI r'AATT_y
Cuts at: 1493 2843 3244 3470 3622 5731 1493
Size: 1350 401 226 152 2109 1697
Fragments arranged by size:
2109 1697 1350 401 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4489 160
Size: 872 3457 1606
Fragments arranged by size:
3457 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
AvaI C'yCGr_G
Cuts at: 1870 1943 2075 2129 3260 1870
Size: 73 132 54 1131 4545
Fragments arranged by size:
4545 1131 132 73 54
BamHI G'GATC_C
Cuts at: 3265 3632 3265
Size: 367 5568
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3256 4711 618
Size: 325 217 627 1469 1455 1842
Fragments arranged by size:
1842 1469 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3254 1391
Size: 1863 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2044 2816 2961 928
Size: 971 145 772 145 3902
Fragments arranged by size:
3902 971 772 145 145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2414 2448 4126 4160 1903
Size: 34 477 34 1678 34 3678
Fragments arranged by size:
3678 1678 477 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3827 5037 3827
Size: 1210 4725
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5935
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4546 136
Size: 107 122 71 631 605 2874 1525
Fragments arranged by size:
2874 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5930 5930
Size: 5935
BlpI GC'TnA_GC
Cuts at: 3352 3352
Size: 5935
Bme1580I G_kGCm'C
Cuts at: 1790 3996 5152 1790
Size: 2206 1156 2573
Fragments arranged by size:
2573 2206 1156
BmgBI CAC'GTC
Cuts at: 1318 3063 1318
Size: 1745 4190
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3666 4624 442
Size: 416 53 83 399 24 2249 958 1753
Fragments arranged by size:
2249 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2951 3014 3139 4595 1239
Size: 1712 63 125 1456 2579
Fragments arranged by size:
2579 1712 1456 125 63
Bpu10I CC'TnA_GC
Cuts at: 2601 3018 2601
Size: 417 5518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2030 4060 4925 5223 2030
Size: 2030 865 298 2742
Fragments arranged by size:
2742 2030 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2203 3144 4598 882
Size: 617 704 941 1454 2219
Fragments arranged by size:
2219 1454 941 704 617
BsaAI yAC'GTr
Cuts at: 493 1204 3004 3726 493
Size: 711 1800 722 2702
Fragments arranged by size:
2702 1800 722 711
BsaBI GATnn'nnATC
Cuts at: 5929 5929
Size: 5935
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3742 4124 4759 275
Size: 53 83 186 936 2209 382 635 1451
Fragments arranged by size:
2209 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3169 4368 4766 5040 5187 5598 3169
Size: 1199 398 274 147 411 3506
Fragments arranged by size:
3506 1199 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2534 2558 2564 2588 3137
Size: 30 165 30 1131 24 6 24 549
Cuts at: 3137 3167 1178
Size: 30 3946
Fragments arranged by size:
3946 1131 549 165 30 30 30 24
24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2284 2650 3212 1186
Size: 1098 366 562 3909
Fragments arranged by size:
3909 1098 562 366
BseYI C'CCAG_C
Cuts at: 2181 2255 5138 5634 2181
Size: 74 2883 496 2482
Fragments arranged by size:
2883 2482 496 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2405 2735 3126 1163
Size: 573 669 330 391 3972
Fragments arranged by size:
3972 669 573 391 330
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3385 4146 5174 1540
Size: 275 186 1384 761 1028 2301
Fragments arranged by size:
2301 1384 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3996 4081 5152 3996
Size: 85 1071 4779
Fragments arranged by size:
4779 1071 85
BsmI GAATG_Cn'
Cuts at: 3446 3539 3446
Size: 93 5842
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3254 3996 4081 5152 1391
Size: 399 1464 742 85 1071 2174
Fragments arranged by size:
2174 1464 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5598 5598
Size: 5935
BspHI T'CATG_A
Cuts at: 3824 5554 3824
Size: 1730 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2787 3044 3290 844
Size: 1943 257 246 3489
Fragments arranged by size:
3489 1943 257 246
BsrBI CCG'CTC
Cuts at: 1394 1823 2128 3822 1394
Size: 429 305 1694 3507
Fragments arranged by size:
3507 1694 429 305
BsrDI GCAATG_nn'
Cuts at: 65 1248 2175 4430 4604 65
Size: 1183 927 2255 174 1396
Fragments arranged by size:
2255 1396 1183 927 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 2212 2724 2748 4579 4766 1700
Size: 110 208 194 512 24 1831 187 2869
Fragments arranged by size:
2869 1831 512 208 194 187 110 24
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5935
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5935
BssSI C'ACGA_G
Cuts at: 2096 3995 5007 2096
Size: 1899 1012 3024
Fragments arranged by size:
3024 1899 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2015 2143 2922 794
Size: 1221 128 779 3807
Fragments arranged by size:
3807 1221 779 128
BstBI TT'CG_AA
Cuts at: 4739 4739
Size: 5935
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5935
BstXI CCAn_nnnn'nTGG
Cuts at: 2977 2992 2977
Size: 15 5920
BstYI r'GATC_y
Cuts at: 1972 3265 3632 4017 4034 5486 5572 5584 5930
Size: 1293 367 385 17 1452 86 12 346
Cuts at: 5930 1972
Size: 1977
Fragments arranged by size:
1977 1452 1293 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4444
Fragments arranged by size:
4444 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5196
Fragments arranged by size:
5196 683 56
BtsI GCAGTG_nn'
Cuts at: 3451 4245 4265 3451
Size: 794 20 5121
Fragments arranged by size:
5121 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5935
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5900
DraI TTT'AAA
Cuts at: 3239 3239
Size: 5935
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3172 4942 809
Size: 507 1856 1770 1802
Fragments arranged by size:
1856 1802 1770 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2535 2750
Size: 54 1425 50 275 18 270 435 215
Cuts at: 2750 2967 3120 3382 4271 5900 8
Size: 217 153 262 889 1629 43
Fragments arranged by size:
1629 1425 889 435 275 270 262 217
215 153 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3382 1537
Size: 275 1570 4090
Fragments arranged by size:
4090 1570 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3865 1353
Size: 2512 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4522 4896 5042 387
Size: 1064 138 2933 374 146 1280
Fragments arranged by size:
2933 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2924 2951 3014 3139 3998 4595 5382
Size: 393 1292 27 63 125 859 597 787
Cuts at: 5382 5650 1239
Size: 268 1524
Fragments arranged by size:
1524 1292 859 787 597 393 268 125
63 27
EcoO109I rG'GnC_Cy
Cuts at: 2347 2347
Size: 5935
EcoRI G'AATT_C
Cuts at: 3244 3622 3244
Size: 378 5557
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 5935
FspI TGC'GCA
Cuts at: 4441 4441
Size: 5935
HaeII r_GCGC'y
Cuts at: 1678 5082 1678
Size: 3404 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2493 2525 4576 4608 4650
Size: 32 135 32 921 32 2051 32 42
Cuts at: 4650 4682 1373
Size: 32 2626
Fragments arranged by size:
2626 2051 921 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2122 2173 2797 3279 3525 669
Size: 988 281 184 51 624 482 246 3079
Fragments arranged by size:
3079 988 624 482 281 246 184 51
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5935
HpaI GTT'AAC
Cuts at: 3525 3525
Size: 5935
KpnI G_GTAC'C
Cuts at: 3260 3260
Size: 5935
MfeI C'AATT_G
Cuts at: 3534 3534
Size: 5935
MluI A'CGCG_T
Cuts at: 5811 5811
Size: 5935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2313 3010 5049 5233 1305
Size: 1008 697 2039 184 2007
Fragments arranged by size:
2039 2007 1008 697 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2537 2969 3122 10
Size: 54 1425 343 705 432 153 2823
Fragments arranged by size:
2823 1425 705 432 343 153 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 2897 3893 4252 4411 518
Size: 648 1065 666 996 359 159 2042
Fragments arranged by size:
2042 1065 996 666 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3150 3351 4028 5176 5421 1606
Size: 1544 201 677 1148 245 2120
Fragments arranged by size:
2120 1544 1148 677 245 201
NaeI GCC'GGC
Cuts at: 2750 2750
Size: 5935
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4444
Fragments arranged by size:
4444 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5935
NgoMIV G'CCGG_C
Cuts at: 2748 2748
Size: 5935
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5935
NotI GC'GGCC_GC
Cuts at: 3382 3382
Size: 5935
NspI r_CATG'y
Cuts at: 1132 1229 2732 3293 4838 1132
Size: 97 1503 561 1545 2229
Fragments arranged by size:
2229 1545 1503 561 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5935
PciI A'CATG_T
Cuts at: 4834 4834
Size: 5935
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2188 1108
Size: 1080 4855
PmeI GTTT'AAAC
Cuts at: 3239 3239
Size: 5935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2534 2566 4004 4036 5517 5549 2534
Size: 32 1438 32 1481 32 2920
Fragments arranged by size:
2920 1481 1438 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5935
PsiI TTA'TAA
Cuts at: 3505 3505
Size: 5935
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2670 2702 2670
Size: 32 5903
PstI C_TGCA'G
Cuts at: 830 2828 3287 830
Size: 1998 459 3478
Fragments arranged by size:
3478 1998 459
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5935
SalI G'TCGA_C
Cuts at: 1936 3277 1936
Size: 1341 4594
SbfI CC_TGCA'GG
Cuts at: 3287 3287
Size: 5935
ScaI AGT'ACT
Cuts at: 4183 5895 4183
Size: 1712 4223
SexAI A'CCwGG_T
Cuts at: 2709 2709
Size: 5935
SfcI C'TryA_G
Cuts at: 826 1045 2687 2824 3283 4418 5099 5290 826
Size: 219 1642 137 459 1135 681 191 1471
Fragments arranged by size:
1642 1471 1135 681 459 219 191 137
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5935
SmaI CCC'GGG
Cuts at: 1872 2077 3262 1872
Size: 205 1185 4545
Fragments arranged by size:
4545 1185 205
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 4039 4940 5202 820
Size: 197 926 102 1994 901 262 1553
Fragments arranged by size:
1994 1553 926 901 262 197 102
SnaBI TAC'GTA
Cuts at: 493 3726 493
Size: 3233 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5935
SphI G_CATG'C
Cuts at: 1132 2732 3293 1132
Size: 1600 561 3774
Fragments arranged by size:
3774 1600 561
SspI AAT'ATT
Cuts at: 5 52 3859 5
Size: 47 3807 2081
Fragments arranged by size:
3807 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5935
StyI C'CwwG_G
Cuts at: 513 1065 2004 2300 2553 2985 3374 513
Size: 552 939 296 253 432 389 3074
Fragments arranged by size:
3074 939 552 432 389 296 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2776 3962 3979 4132 4317 4762 325
Size: 1255 1196 1186 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1196 1186 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3144 4181 5893
Size: 274 80 33 51 1443 1167 1037 1712
Cuts at: 5893 96
Size: 138
Fragments arranged by size:
1712 1443 1167 1037 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2558 2590 5895 5927 2558
Size: 32 3305 32 2566
Fragments arranged by size:
3305 2566 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5935
XbaI T'CTAG_A
Cuts at: 3271 3271
Size: 5935
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5935
XmaI C'CCGG_G
Cuts at: 1870 2075 3260 1870
Size: 205 1185 4545
Fragments arranged by size:
4545 1185 205
XmnI GAAnn'nnTTC
Cuts at: 2682 4064 2682
Size: 1382 4553
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3743 276
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI