Restriction map for
pFN21AB7685
(Circular) (Six-base) MAPSORT of: pFN21AB7685.seq Check: 3964 from: 1 to: 5749
pFN21AB7685 5749 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3092 1937
Size: 1155 4594
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2119 2890 3812 5196 5464 1632
Size: 487 771 922 1384 268 1917
Fragments arranged by size:
1917 1384 922 771 487 268
AfeI AGC'GCT
Cuts at: 2831 2831
Size: 5749
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5552
AflIII A'CryG_T
Cuts at: 1203 4648 5625 1203
Size: 3445 977 1327
Fragments arranged by size:
3445 1327 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 2095 2949 1166
Size: 929 854 3966
Fragments arranged by size:
3966 929 854
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AlwNI CAG_nnn'CTG
Cuts at: 2212 2272 2327 3492 5064 2212
Size: 60 55 1165 1572 2897
Fragments arranged by size:
2897 1572 1165 60 55
ApaLI G'TGCA_C
Cuts at: 2310 3806 4962 2310
Size: 1496 1156 3097
Fragments arranged by size:
3097 1496 1156
ApoI r'AATT_y
Cuts at: 1493 2594 3024 3058 3284 3436 5545 1493
Size: 1101 430 34 226 152 2109 1697
Fragments arranged by size:
2109 1697 1101 430 226 152 34
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 3074 1870
Size: 73 1131 4545
Fragments arranged by size:
4545 1131 73
BamHI G'GATC_C
Cuts at: 3079 3446 3079
Size: 367 5382
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3070 4525 618
Size: 325 217 627 1283 1455 1842
Fragments arranged by size:
1842 1455 1283 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2199 3068 1391
Size: 808 869 4072
Fragments arranged by size:
4072 869 808
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2710 928
Size: 971 811 3967
Fragments arranged by size:
3967 971 811
BbvCI CC'TCA_GC
Cuts at: 2213 2637 2213
Size: 424 5325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3940 3974 1903
Size: 34 2003 34 3678
Fragments arranged by size:
3678 2003 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4360 136
Size: 107 122 71 631 605 2688 1525
Fragments arranged by size:
2688 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5744 5744
Size: 5749
BlpI GC'TnA_GC
Cuts at: 3166 3166
Size: 5749
Bme1580I G_kGCm'C
Cuts at: 1790 2314 3810 4966 1790
Size: 524 1496 1156 2573
Fragments arranged by size:
2573 1496 1156 524
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2080 3480 4438
Size: 416 53 83 399 24 663 1400 958
Cuts at: 4438 442
Size: 1753
Fragments arranged by size:
1753 1400 958 663 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2349 2372 2381 2519 4409 1239
Size: 1110 23 9 138 1890 2579
Fragments arranged by size:
2579 1890 1110 138 23 9
Bpu10I CC'TnA_GC
Cuts at: 2213 2273 2637 2213
Size: 60 364 5325
Fragments arranged by size:
5325 364 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3874 4739 5037 3874
Size: 865 298 4586
Fragments arranged by size:
4586 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4412 882
Size: 617 2913 2219
Fragments arranged by size:
2913 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3540 493
Size: 711 2336 2702
Fragments arranged by size:
2702 2336 711
BsaBI GATnn'nnATC
Cuts at: 5743 5743
Size: 5749
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3556 3938 4573 275
Size: 53 83 186 936 2023 382 635 1451
Fragments arranged by size:
2023 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5524
Fragments arranged by size:
5524 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2625 1186
Size: 1162 277 4310
Fragments arranged by size:
4310 1162 277
BseYI C'CCAG_C
Cuts at: 2184 2493 2510 4952 5448 2184
Size: 309 17 2442 496 2485
Fragments arranged by size:
2485 2442 496 309 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2119 1163
Size: 573 383 4793
Fragments arranged by size:
4793 573 383
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3199 3960 4988 1540
Size: 275 186 1198 761 1028 2301
Fragments arranged by size:
2301 1198 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2199 2314 3810 3895 4966 2199
Size: 115 1496 85 1071 2982
Fragments arranged by size:
2982 1496 1071 115 85
BsmI GAATG_Cn'
Cuts at: 2228 2277 3260 3353 2228
Size: 49 983 93 4624
Fragments arranged by size:
4624 983 93 49
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2199 2314 3068 3810 3895 4966 1391
Size: 399 409 115 754 742 85 1071 2174
Fragments arranged by size:
2174 1071 754 742 409 399 115 85
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 3638 5368 3638
Size: 1730 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2259 2301 2380 3104 844
Size: 1415 42 79 724 3489
Fragments arranged by size:
3489 1415 724 79 42
BsrBI CCG'CTC
Cuts at: 1394 1823 3636 1394
Size: 429 1813 3507
Fragments arranged by size:
3507 1813 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4244 4418 65
Size: 1183 2996 174 1396
Fragments arranged by size:
2996 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4393 4580 1700
Size: 110 2583 187 2869
Fragments arranged by size:
2869 2583 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5749
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5749
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5749
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 2017
Size: 5749
BstYI r'GATC_y
Cuts at: 1972 2356 3079 3446 3831 3848 5300 5386 5398
Size: 384 723 367 385 17 1452 86 12
Cuts at: 5398 5744 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 723 385 384 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2025 2025
Size: 5749
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5010
Fragments arranged by size:
5010 683 56
BtsI GCAGTG_nn'
Cuts at: 2346 3265 4059 4079 2346
Size: 919 794 20 4016
Fragments arranged by size:
4016 919 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4756 809
Size: 507 3440 1802
Fragments arranged by size:
3440 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3196 4085 5714
Size: 54 1425 50 275 18 1366 889 1629
Cuts at: 5714 8
Size: 43
Fragments arranged by size:
1629 1425 1366 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3196 1537
Size: 275 1384 4090
Fragments arranged by size:
4090 1384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2550 3679 1353
Size: 1197 1129 3423
Fragments arranged by size:
3423 1197 1129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4336 4710 4856 387
Size: 1064 138 2747 374 146 1280
Fragments arranged by size:
2747 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2197 2197
Size: 5749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2119 2349 2372 2381 2519 2890 3812
Size: 393 487 230 23 9 138 371 922
Cuts at: 3812 4409 5196 5464 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 922 787 597 487 393 371 268
230 138 23 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2446 2446
Size: 5749
EcoO109I rG'GnC_Cy
Cuts at: 2392 2392
Size: 5749
EcoRI G'AATT_C
Cuts at: 2594 3024 3058 3436 2594
Size: 430 34 378 4907
Fragments arranged by size:
4907 430 378 34
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 2833 4896 1678
Size: 1155 2063 2531
Fragments arranged by size:
2531 2063 1155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2072 2104 2663 2695 4390
Size: 32 135 32 500 32 559 32 1695
Cuts at: 4390 4422 4464 4496 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1695 559 500 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3093 3339 669
Size: 988 281 1155 246 3079
Fragments arranged by size:
3079 1155 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5749
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4863 5047 1305
Size: 3558 184 2007
Fragments arranged by size:
3558 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3927
Fragments arranged by size:
3927 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2095 2423 2949 3707 4066 4225 518
Size: 648 929 328 526 758 359 159 2042
Fragments arranged by size:
2042 929 758 648 526 359 328 159
MspA1I CmG'CkG
Cuts at: 1606 2188 2497 3165 3842 4990 5235 1606
Size: 582 309 668 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 668 582 309 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5197
NdeI CA'TA_TG
Cuts at: 387 2455 387
Size: 2068 3681
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 3107 4652 1132
Size: 97 1878 1545 2229
Fragments arranged by size:
2229 1878 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5749
PciI A'CATG_T
Cuts at: 4648 4648
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2327 1108
Size: 1219 4530
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 5331 5363 3818
Size: 32 1481 32 4204
Fragments arranged by size:
4204 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2392 2392
Size: 5749
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5749
PsiI TTA'TAA
Cuts at: 3319 3319
Size: 5749
PstI C_TGCA'G
Cuts at: 830 3101 830
Size: 2271 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
PvuII CAG'CTG
Cuts at: 2188 2497 2188
Size: 309 5440
SacI G_AGCT'C
Cuts at: 2199 2199
Size: 5749
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SbfI CC_TGCA'GG
Cuts at: 3101 3101
Size: 5749
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SfcI C'TryA_G
Cuts at: 826 1045 2122 3097 4232 4913 5104 826
Size: 219 1077 975 1135 681 191 1471
Fragments arranged by size:
1471 1135 1077 975 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 3076 1872
Size: 1204 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3853 4754 5016 820
Size: 197 926 1910 901 262 1553
Fragments arranged by size:
1910 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 3107 1132
Size: 1975 3774
SspI AAT'ATT
Cuts at: 5 52 3673 5
Size: 47 3621 2081
Fragments arranged by size:
3621 2081 47
StuI AGG'CCT
Cuts at: 1517 2986 1517
Size: 1469 4280
StyI C'CwwG_G
Cuts at: 513 1065 2232 2442 2599 3188 513
Size: 552 1167 210 157 589 3074
Fragments arranged by size:
3074 1167 589 552 210 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2293 3776 3793 3946 4131 4576 325
Size: 1255 713 1483 17 153 185 445 1498
Fragments arranged by size:
1498 1483 1255 713 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2857 3995 5707
Size: 274 80 33 51 1443 880 1138 1712
Cuts at: 5707 96
Size: 138
Fragments arranged by size:
1712 1443 1138 880 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2454 2486 5709 5741 2454
Size: 32 3223 32 2462
Fragments arranged by size:
3223 2462 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 3085 3085
Size: 5749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2324 2324
Size: 5749
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 3074 1870
Size: 1204 4545
XmnI GAAnn'nnTTC
Cuts at: 2735 2903 3878 2735
Size: 168 975 4606
Fragments arranged by size:
4606 975 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI