Restriction map for
pFN21AB7683
(Circular) (Six-base) MAPSORT of: pFN21AB7683.seq Check: 8013 from: 1 to: 5743
pFN21AB7683 5743 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2238 3553 278
Size: 53 83 186 936 702 1315 2468
Fragments arranged by size:
2468 1315 936 702 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2922 3086 1937
Size: 985 164 4594
Fragments arranged by size:
4594 985 164
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2832 3806 5190 5458 1632
Size: 1200 974 1384 268 1917
Fragments arranged by size:
1917 1384 1200 974 268
AfeI AGC'GCT
Cuts at: 2822 2822
Size: 5743
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 4642 5619 1203
Size: 3439 977 1327
Fragments arranged by size:
3439 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 2309 3486 5058 2309
Size: 1177 1572 2994
Fragments arranged by size:
2994 1572 1177
ApaI G_GGCC'C
Cuts at: 2195 2195
Size: 5743
ApaLI G'TGCA_C
Cuts at: 2687 3800 4956 2687
Size: 1113 1156 3474
Fragments arranged by size:
3474 1156 1113
ApoI r'AATT_y
Cuts at: 1493 2110 3052 3278 3430 5539 1493
Size: 617 942 226 152 2109 1697
Fragments arranged by size:
2109 1697 942 617 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 3068 1870
Size: 73 1125 4545
Fragments arranged by size:
4545 1125 73
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3064 4519 618
Size: 325 217 627 1277 1455 1842
Fragments arranged by size:
1842 1455 1277 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2195 2796 3062 1391
Size: 804 601 266 4072
Fragments arranged by size:
4072 804 601 266
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2949 928
Size: 971 1050 3722
Fragments arranged by size:
3722 1050 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 1903
Size: 34 1997 34 3678
Fragments arranged by size:
3678 1997 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3635 4845 3635
Size: 1210 4533
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4354 136
Size: 107 122 71 631 605 2682 1525
Fragments arranged by size:
2682 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2216 5738 2216
Size: 3522 2221
BlpI GC'TnA_GC
Cuts at: 3160 3160
Size: 5743
Bme1580I G_kGCm'C
Cuts at: 1790 2195 2691 3804 4960 1790
Size: 405 496 1113 1156 2573
Fragments arranged by size:
2573 1156 1113 496 405
BmgBI CAC'GTC
Cuts at: 1318 2677 1318
Size: 1359 4384
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3474 4432 442
Size: 416 53 83 399 24 2057 958 1753
Fragments arranged by size:
2057 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 2996
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4403 1239
Size: 3164 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2146 2966 3868 4733 5031 2146
Size: 820 902 865 298 2858
Fragments arranged by size:
2858 902 865 820 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4406 882
Size: 617 2907 2219
Fragments arranged by size:
2907 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3534 493
Size: 711 2330 2702
Fragments arranged by size:
2702 2330 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2235 3550 3932 4567
Size: 53 83 186 936 702 1315 382 635
Cuts at: 4567 275
Size: 1451
Fragments arranged by size:
1451 1315 936 702 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2015 4176 4574 4848 4995 5406 2015
Size: 2161 398 274 147 411 2352
Fragments arranged by size:
2352 2161 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5518
Fragments arranged by size:
5518 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5743
BseYI C'CCAG_C
Cuts at: 4946 5442 4946
Size: 496 5247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5170
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3193 3954 4982 1540
Size: 275 186 1192 761 1028 2301
Fragments arranged by size:
2301 1192 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2691 3804 3889 4960 2691
Size: 1113 85 1071 3474
Fragments arranged by size:
3474 1113 1071 85
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
BsmBI CGTCTCn'nnnn_
Cuts at: 2042 2042
Size: 5743
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2195 2691 2796 3062 3804 3889 4960
Size: 399 405 496 105 266 742 85 1071
Cuts at: 4960 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 496 405 399 266 105
85
BspEI T'CCGG_A
Cuts at: 2015 5406 2015
Size: 3391 2352
BspHI T'CATG_A
Cuts at: 3632 5362 3632
Size: 1730 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3098 844
Size: 2254 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 1394
Size: 429 1807 3507
Fragments arranged by size:
3507 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2623 4238 4412 65
Size: 1183 1375 1615 174 1396
Fragments arranged by size:
1615 1396 1375 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4387 4574 1700
Size: 110 2577 187 2869
Fragments arranged by size:
2869 2577 187 110
BsrGI T'GTAC_A
Cuts at: 96 2410 96
Size: 2314 3429
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 3803 4815 3803
Size: 1012 4731
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5743
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstYI r'GATC_y
Cuts at: 1972 2216 3073 3440 3825 3842 5294 5380 5392
Size: 244 857 367 385 17 1452 86 12
Cuts at: 5392 5738 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 857 385 367 346 244 86
17 12
BstZ17I GTA'TAC
Cuts at: 2923 2923
Size: 5743
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 3259 4053 4073 3259
Size: 794 20 4929
Fragments arranged by size:
4929 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4750 809
Size: 507 3434 1802
Fragments arranged by size:
3434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3190 4079 5708
Size: 54 1425 50 275 18 1360 889 1629
Cuts at: 5708 8
Size: 43
Fragments arranged by size:
1629 1425 1360 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3190 1537
Size: 275 1378 4090
Fragments arranged by size:
4090 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2877 3009 3673 1353
Size: 1524 132 664 3423
Fragments arranged by size:
3423 1524 664 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4330 4704 4850 387
Size: 1064 138 2741 374 146 1280
Fragments arranged by size:
2741 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2832 3806 4403 5190 5458 1239
Size: 393 1200 974 597 787 268 1524
Fragments arranged by size:
1524 1200 974 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2199 2199
Size: 5743
EcoO109I rG'GnC_Cy
Cuts at: 2192 2192
Size: 5743
EcoRI G'AATT_C
Cuts at: 3052 3430 3052
Size: 378 5365
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 2824 4890 1678
Size: 1146 2066 2531
Fragments arranged by size:
2531 2066 1146
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4384 4416 4458 4490 1373
Size: 32 135 32 2812 32 42 32 2626
Fragments arranged by size:
2812 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3333 669
Size: 988 281 1149 246 3079
Fragments arranged by size:
3079 1149 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2649 748
Size: 1901 3842
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4857 5041 1305
Size: 3552 184 2007
Fragments arranged by size:
3552 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3921
Fragments arranged by size:
3921 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2334 3701 4060 4219 518
Size: 648 1168 1367 359 159 2042
Fragments arranged by size:
2042 1367 1168 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3159 3836 4984 5229 1606
Size: 1553 677 1148 245 2120
Fragments arranged by size:
2120 1553 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 3101 4646 1132
Size: 97 1872 1545 2229
Fragments arranged by size:
2229 1872 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2195 2388 1348
Size: 847 193 4703
Fragments arranged by size:
4703 847 193
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5743
PfoI T'CCnGG_A
Cuts at: 2118 2278 2641 2118
Size: 160 363 5220
Fragments arranged by size:
5220 363 160
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PspOMI G'GGCC_C
Cuts at: 2191 2191
Size: 5743
PstI C_TGCA'G
Cuts at: 830 3095 830
Size: 2265 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 3991 5703 3991
Size: 1712 4031
SfcI C'TryA_G
Cuts at: 826 1045 2482 3091 4226 4907 5098 826
Size: 219 1437 609 1135 681 191 1471
Fragments arranged by size:
1471 1437 1135 681 609 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 3070 1872
Size: 1198 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2125 2981 3847 4748 5010 820
Size: 197 926 182 856 866 901 262 1553
Fragments arranged by size:
1553 926 901 866 856 262 197 182
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 3101 1132
Size: 1969 3774
SspI AAT'ATT
Cuts at: 5 52 2476 3667 5
Size: 47 2424 1191 2081
Fragments arranged by size:
2424 2081 1191 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5743
StyI C'CwwG_G
Cuts at: 513 1065 2004 3182 513
Size: 552 939 1178 3074
Fragments arranged by size:
3074 1178 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3770 3787 3940 4125 4570 325
Size: 1255 2190 17 153 185 445 1498
Fragments arranged by size:
2190 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2410 3989 5701
Size: 274 80 33 51 1443 433 1579 1712
Cuts at: 5701 96
Size: 138
Fragments arranged by size:
1712 1579 1443 433 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2097 2129 5703 5735 2097
Size: 32 3574 32 2105
Fragments arranged by size:
3574 2105 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2668 2668
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 3068 1870
Size: 1198 4545
XmnI GAAnn'nnTTC
Cuts at: 3872 3872
Size: 5743
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2236 3551 276
Size: 53 83 186 936 702 1315 2468
Fragments arranged by size:
2468 1315 936 702 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI