Restriction map for
pFN21AB7682
(Circular) (Six-base) MAPSORT of: pFN21AB7682.seq Check: 1430 from: 1 to: 5923
pFN21AB7682 5923 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2809 2936 2809
Size: 127 5796
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2395 3177 3266 1937
Size: 458 782 89 4594
Fragments arranged by size:
4594 782 458 89
Acc65I G'GTAC_C
Cuts at: 2344 3244 2344
Size: 900 5023
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3138 3986 5370 5638 1632
Size: 1506 848 1384 268 1917
Fragments arranged by size:
1917 1506 1384 848 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 4822 5799 1203
Size: 3619 977 1327
Fragments arranged by size:
3619 1327 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2315 1184
Size: 1131 4792
AlwNI CAG_nnn'CTG
Cuts at: 2515 3666 5238 2515
Size: 1151 1572 3200
Fragments arranged by size:
3200 1572 1151
ApaI G_GGCC'C
Cuts at: 2162 2381 2432 2561 2627 2964 2162
Size: 219 51 129 66 337 5121
Fragments arranged by size:
5121 337 219 129 66 51
ApaLI G'TGCA_C
Cuts at: 3076 3980 5136 3076
Size: 904 1156 3863
Fragments arranged by size:
3863 1156 904
ApoI r'AATT_y
Cuts at: 1493 3232 3458 3610 5719 1493
Size: 1739 226 152 2109 1697
Fragments arranged by size:
2109 1739 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 3248 1870
Size: 73 1305 4545
Fragments arranged by size:
4545 1305 73
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2344 2929 2996 3244
Size: 325 217 627 230 327 585 67 248
Cuts at: 3244 4699 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 585 327 325 248 230
217 67
BanII G_rGCy'C
Cuts at: 1391 2162 2257 2381 2432 2561 2627 2665 2706
Size: 771 95 124 51 129 66 38 41
Cuts at: 2706 2829 2957 2964 3242 1391
Size: 123 128 7 278 4072
Fragments arranged by size:
4072 771 278 129 128 124 123 95
66 51 41 38 7
BbeI G_GCGC'C
Cuts at: 2021 2021
Size: 5923
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4952
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4114 4148 1903
Size: 34 2177 34 3678
Fragments arranged by size:
3678 2177 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 3134 4534
Size: 107 122 71 631 605 343 1119 1400
Cuts at: 4534 136
Size: 1525
Fragments arranged by size:
1525 1400 1119 631 605 343 122 107
71
BglII A'GATC_T
Cuts at: 5918 5918
Size: 5923
BlpI GC'TnA_GC
Cuts at: 2652 3340 2652
Size: 688 5235
Bme1580I G_kGCm'C
Cuts at: 1790 2162 2304 2381 2432 2561 2627 2909 2964
Size: 372 142 77 51 129 66 282 55
Cuts at: 2964 3080 3984 5140 1790
Size: 116 904 1156 2573
Fragments arranged by size:
2573 1156 904 372 282 142 129 116
77 66 55 51
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2532 2555 2865
Size: 416 53 83 399 24 1115 23 310
Cuts at: 2865 3654 4612 442
Size: 789 958 1753
Fragments arranged by size:
1753 1115 958 789 416 399 310 83
53 24 23
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2848 2848
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2385 2669 2895 4583 1239
Size: 821 325 284 226 1688 2579
Fragments arranged by size:
2579 1688 821 325 284 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3137 4048 4913 5211 3137
Size: 911 865 298 3849
Fragments arranged by size:
3849 911 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2683 4586 882
Size: 617 1184 1903 2219
Fragments arranged by size:
2219 1903 1184 617
BsaAI yAC'GTr
Cuts at: 493 1204 3714 493
Size: 711 2510 2702
Fragments arranged by size:
2702 2510 711
BsaBI GATnn'nnATC
Cuts at: 5917 5917
Size: 5923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 3730 4112 4747
Size: 53 83 186 936 485 1712 382 635
Cuts at: 4747 275
Size: 1451
Fragments arranged by size:
1712 1451 936 635 485 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2029 2743 4356 4754 5028 5175 5586 2029
Size: 714 1613 398 274 147 411 2366
Fragments arranged by size:
2366 1613 714 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2822 2852 3155 3185 1178
Size: 30 165 30 1419 30 303 30 3916
Fragments arranged by size:
3916 1419 303 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2670 1186
Size: 1484 4439
BseYI C'CCAG_C
Cuts at: 2078 2382 2492 2620 2708 2964 5126 5622 2078
Size: 304 110 128 88 256 2162 496 2379
Fragments arranged by size:
2379 2162 496 304 256 128 110 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2342 1163
Size: 573 606 4744
Fragments arranged by size:
4744 606 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 4134 5162 1540
Size: 275 186 1372 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3080 3984 4069 5140 3080
Size: 904 85 1071 3863
Fragments arranged by size:
3863 1071 904 85
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 2257 2304 2381 2432 2561 2627
Size: 399 372 95 47 77 51 129 66
Cuts at: 2627 2665 2706 2829 2909 2957 2964 3080 3242
Size: 38 41 123 80 48 7 116 162
Cuts at: 3242 3984 4069 5140 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 372 162 129 123
116 95 85 80 77 66 51 48
47 41 38 7
BspEI T'CCGG_A
Cuts at: 2743 5586 2743
Size: 2843 3080
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2809 2936 3278 844
Size: 1965 127 342 3489
Fragments arranged by size:
3489 1965 342 127
BsrBI CCG'CTC
Cuts at: 1394 1823 3810 1394
Size: 429 1987 3507
Fragments arranged by size:
3507 1987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4418 4592 65
Size: 1183 3170 174 1396
Fragments arranged by size:
3170 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2105 4567 4754 1700
Size: 110 295 2462 187 2869
Fragments arranged by size:
2869 2462 295 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2551 794
Size: 1757 4166
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstXI CCAn_nnnn'nTGG
Cuts at: 2425 2459 2693 2926 2425
Size: 34 234 233 5422
Fragments arranged by size:
5422 234 233 34
BstYI r'GATC_y
Cuts at: 1972 3165 3253 3620 4005 4022 5474 5560 5572
Size: 1193 88 367 385 17 1452 86 12
Cuts at: 5572 5918 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1193 385 367 346 88 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2205 2595 513
Size: 552 939 201 390 3841
Fragments arranged by size:
3841 939 552 390 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 2896 3439 4233 4253 2896
Size: 543 794 20 4566
Fragments arranged by size:
4566 794 543 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2929 2964 529
Size: 35 2365 35 3488
Fragments arranged by size:
3488 2365 35 35
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3370 4259
Size: 54 1425 50 275 18 177 1363 889
Cuts at: 4259 5888 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1363 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2114 3853 1353
Size: 761 1739 3423
Fragments arranged by size:
3423 1739 761
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4510 4884 5030 387
Size: 1064 138 2921 374 146 1280
Fragments arranged by size:
2921 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2385 2669 2895 3138 3986 4583
Size: 393 428 325 284 226 243 848 597
Cuts at: 4583 5370 5638 1239
Size: 787 268 1524
Fragments arranged by size:
1524 848 787 597 428 393 325 284
268 243 226
EcoNI CCTnn'n_nnAGG
Cuts at: 2973 2973
Size: 5923
EcoO109I rG'GnC_Cy
Cuts at: 2158 2159 2377 2378 2429 2456 2961 2158
Size: 1 218 1 51 27 505 5120
Fragments arranged by size:
5120 505 218 51 27 1 1
EcoRI G'AATT_C
Cuts at: 3232 3610 3232
Size: 378 5545
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 2021 3099 5070 1678
Size: 343 1078 1971 2531
Fragments arranged by size:
2531 1971 1078 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2680 2712 3155 3187 4564
Size: 32 135 32 1108 32 443 32 1377
Cuts at: 4564 4596 4638 4670 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1377 1108 443 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2396 3267 3513 669
Size: 988 281 458 871 246 3079
Fragments arranged by size:
3079 988 871 458 281 246
HindIII A'AGCT_T
Cuts at: 748 3120 748
Size: 2372 3551
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KasI G'GCGC_C
Cuts at: 2017 2017
Size: 5923
KpnI G_GTAC'C
Cuts at: 2348 3248 2348
Size: 900 5023
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2253 2984 5037 5221 1305
Size: 948 731 2053 184 2007
Fragments arranged by size:
2053 2007 948 731 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4101
Fragments arranged by size:
4101 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3083 3881 4240 4399 518
Size: 648 1917 798 359 159 2042
Fragments arranged by size:
2042 1917 798 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3202 3339 4016 5164 5409 1606
Size: 1596 137 677 1148 245 2120
Fragments arranged by size:
2120 1596 1148 677 245 137
NarI GG'CG_CC
Cuts at: 2018 2018
Size: 5923
NcoI C'CATG_G
Cuts at: 513 1065 2004 2205 2595 513
Size: 552 939 201 390 3841
Fragments arranged by size:
3841 939 552 390 201
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 2446 3281 4826 1132
Size: 97 1217 835 1545 2229
Fragments arranged by size:
2229 1545 1217 835 97
PasI CC'CwG_GG
Cuts at: 1348 2452 1348
Size: 1104 4819
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2288 2515 1108
Size: 1180 227 4516
Fragments arranged by size:
4516 1180 227
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2456 2456
Size: 5923
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PspOMI G'GGCC_C
Cuts at: 2158 2377 2428 2557 2623 2960 2158
Size: 219 51 129 66 337 5121
Fragments arranged by size:
5121 337 219 129 66 51
PstI C_TGCA'G
Cuts at: 830 3275 830
Size: 2445 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
PvuII CAG'CTG
Cuts at: 3202 3202
Size: 5923
SalI G'TCGA_C
Cuts at: 1936 2394 3265 1936
Size: 458 871 4594
Fragments arranged by size:
4594 871 458
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SexAI A'CCwGG_T
Cuts at: 2217 2692 2217
Size: 475 5448
SfcI C'TryA_G
Cuts at: 826 1045 2356 3271 4406 5087 5278 826
Size: 219 1311 915 1135 681 191 1471
Fragments arranged by size:
1471 1311 1135 915 681 219 191
SfoI GGC'GCC
Cuts at: 2019 2019
Size: 5923
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 3250 1872
Size: 1378 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3152 4027 4928 5190 820
Size: 197 926 1209 875 901 262 1553
Fragments arranged by size:
1553 1209 926 901 875 262 197
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 3047 1517
Size: 1530 4393
StyI C'CwwG_G
Cuts at: 513 1065 2004 2205 2287 2595 3159 3362 513
Size: 552 939 201 82 308 564 203 3074
Fragments arranged by size:
3074 939 564 552 308 203 201 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2605 3950 3967 4120 4305 4750 325
Size: 1255 1025 1345 17 153 185 445 1498
Fragments arranged by size:
1498 1345 1255 1025 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4169 5881 96
Size: 274 80 33 51 1443 2192 1712 138
Fragments arranged by size:
2192 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2394 2426 2822 2854 5883 5915 2394
Size: 32 396 32 3029 32 2402
Fragments arranged by size:
3029 2402 396 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2694 1316
Size: 1378 4545
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2617 2617
Size: 5923
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 3248 1870
Size: 1378 4545
XmnI GAAnn'nnTTC
Cuts at: 3062 4052 3062
Size: 990 4933
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SrfI SwaI