Restriction map for
pFN21AB7681
(Circular) (Six-base) MAPSORT of: pFN21AB7681.seq Check: 5141 from: 1 to: 5734
pFN21AB7681 5734 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2124 2124
Size: 5734
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3544 278
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3077 1937
Size: 1140 4594
Acc65I G'GTAC_C
Cuts at: 3055 3055
Size: 5734
AclI AA'CG_TT
Cuts at: 1563 3861 4234 1563
Size: 2298 373 3063
Fragments arranged by size:
3063 2298 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2467 3797 5181 5449 1632
Size: 835 1330 1384 268 1917
Fragments arranged by size:
1917 1384 1330 835 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5537
AflIII A'CryG_T
Cuts at: 1203 4633 5610 1203
Size: 3430 977 1327
Fragments arranged by size:
3430 1327 977
AgeI A'CCGG_T
Cuts at: 4565 4565
Size: 5734
AhdI GACnn_n'nnGTC
Cuts at: 2965 4463 2965
Size: 1498 4236
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5734
AlwNI CAG_nnn'CTG
Cuts at: 2759 3477 5049 2759
Size: 718 1572 3444
Fragments arranged by size:
3444 1572 718
ApaI G_GGCC'C
Cuts at: 2087 2172 2235 2273 2345 2358 2087
Size: 85 63 38 72 13 5463
Fragments arranged by size:
5463 85 72 63 38 13
ApaLI G'TGCA_C
Cuts at: 3791 4947 3791
Size: 1156 4578
ApoI r'AATT_y
Cuts at: 1493 3043 3269 3421 5530 1493
Size: 1550 226 152 2109 1697
Fragments arranged by size:
2109 1697 1550 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4288 160
Size: 872 3256 1606
Fragments arranged by size:
3256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 2065 2228 2416 2550 2589 3059
Size: 73 69 53 163 188 134 39 470
Cuts at: 3059 1870
Size: 4545
Fragments arranged by size:
4545 470 188 163 134 73 69 53
39
BamHI G'GATC_C
Cuts at: 3064 3431 3064
Size: 367 5367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2863 3055 4510 618
Size: 325 217 627 1076 192 1455 1842
Fragments arranged by size:
1842 1455 1076 627 325 217 192
BanII G_rGCy'C
Cuts at: 1391 2087 2172 2181 2235 2273 2345 2358 2768
Size: 696 85 9 54 38 72 13 410
Cuts at: 2768 3053 1391
Size: 285 4072
Fragments arranged by size:
4072 696 410 285 85 72 54 38
13 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2297 2509 928
Size: 971 398 212 4153
Fragments arranged by size:
4153 971 398 212
BbvCI CC'TCA_GC
Cuts at: 2283 2724 2283
Size: 441 5293
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3925 3959 1903
Size: 34 1988 34 3678
Fragments arranged by size:
3678 1988 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3626 4836 3626
Size: 1210 4524
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5734
BfrBI ATG'CAT
Cuts at: 2814 2814
Size: 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2340 2772 4345
Size: 107 122 71 631 605 668 432 1573
Cuts at: 4345 136
Size: 1525
Fragments arranged by size:
1573 1525 668 631 605 432 122 107
71
BglII A'GATC_T
Cuts at: 5729 5729
Size: 5734
BlpI GC'TnA_GC
Cuts at: 3151 3151
Size: 5734
Bme1580I G_kGCm'C
Cuts at: 1790 2087 2172 2235 2273 2345 2358 3795 4951
Size: 297 85 63 38 72 13 1437 1156
Cuts at: 4951 1790
Size: 2573
Fragments arranged by size:
2573 1437 1156 297 85 72 63 38
13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5734
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3465 4423 442
Size: 416 53 83 399 24 2048 958 1753
Fragments arranged by size:
2048 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5734
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2311 2965 2311
Size: 654 5080
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 2519 2528 2775 2790 4394 1239
Size: 956 324 9 247 15 1604 2579
Fragments arranged by size:
2579 1604 956 324 247 15 9
Bpu10I CC'TnA_GC
Cuts at: 2283 2724 2987 2283
Size: 441 263 5030
Fragments arranged by size:
5030 441 263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3859 4724 5022 3859
Size: 865 298 4571
Fragments arranged by size:
4571 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4397 882
Size: 617 2898 2219
Fragments arranged by size:
2898 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3525 493
Size: 711 2321 2702
Fragments arranged by size:
2702 2321 711
BsaBI GATnn'nnATC
Cuts at: 5728 5728
Size: 5734
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3541 3923 4558 275
Size: 53 83 186 936 2008 382 635 1451
Fragments arranged by size:
2008 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4167 4565 4839 4986 5397 4167
Size: 398 274 147 411 4504
Fragments arranged by size:
4504 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5509
Fragments arranged by size:
5509 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2300 2516 1186
Size: 1114 216 4404
Fragments arranged by size:
4404 1114 216
BseYI C'CCAG_C
Cuts at: 2316 4937 5433 2316
Size: 2621 496 2617
Fragments arranged by size:
2621 2617 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2028 2102 1163
Size: 573 292 74 4795
Fragments arranged by size:
4795 573 292 74
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3184 3945 4973 1540
Size: 275 186 1183 761 1028 2301
Fragments arranged by size:
2301 1183 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2019 3795 3880 4951 2019
Size: 1776 85 1071 2802
Fragments arranged by size:
2802 1776 1071 85
BsmI GAATG_Cn'
Cuts at: 3245 3338 3245
Size: 93 5641
BsmBI CGTCTCn'nnnn_
Cuts at: 2343 2986 2343
Size: 643 5091
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2087 2172 2181 2235 2273 2345
Size: 399 229 68 85 9 54 38 72
Cuts at: 2345 2358 2768 3053 3795 3880 4951 1391
Size: 13 410 285 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 410 399 285 229 85
85 72 68 54 38 13 9
BspEI T'CCGG_A
Cuts at: 5397 5397
Size: 5734
BspHI T'CATG_A
Cuts at: 3623 5353 3623
Size: 1730 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2124 2226 3089 844
Size: 1280 102 863 3489
Fragments arranged by size:
3489 1280 863 102
BsrBI CCG'CTC
Cuts at: 1394 1823 3621 1394
Size: 429 1798 3507
Fragments arranged by size:
3507 1798 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4229 4403 65
Size: 1183 2981 174 1396
Fragments arranged by size:
2981 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2194 4378 4565 1700
Size: 110 384 2184 187 2869
Fragments arranged by size:
2869 2184 384 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5734
BssHII G'CGCG_C
Cuts at: 1925 2022 1925
Size: 97 5637
BssSI C'ACGA_G
Cuts at: 3794 4806 3794
Size: 1012 4722
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5734
BstBI TT'CG_AA
Cuts at: 4538 4538
Size: 5734
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5734
BstXI CCAn_nnnn'nTGG
Cuts at: 2440 2837 2440
Size: 397 5337
BstYI r'GATC_y
Cuts at: 1972 2652 2756 3064 3431 3816 3833 5285 5371
Size: 680 104 308 367 385 17 1452 86
Cuts at: 5371 5383 5729 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 680 385 367 346 308 104
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2134 2141 2274 2433 2836 2905
Size: 552 939 130 7 133 159 403 69
Cuts at: 2905 3030 513
Size: 125 3217
Fragments arranged by size:
3217 939 552 403 159 133 130 125
69 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4995
Fragments arranged by size:
4995 683 56
BtsI GCAGTG_nn'
Cuts at: 2017 3250 4044 4064 2017
Size: 1233 794 20 3687
Fragments arranged by size:
3687 1233 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5699
DraI TTT'AAA
Cuts at: 3038 3038
Size: 5734
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4741 809
Size: 507 3425 1802
Fragments arranged by size:
3425 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2254 2391
Size: 54 1425 50 275 18 366 58 137
Cuts at: 2391 2442 3181 4070 5699 8
Size: 51 739 889 1629 43
Fragments arranged by size:
1629 1425 889 739 366 275 137 58
54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3181 1537
Size: 275 1369 4090
Fragments arranged by size:
4090 1369 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3664 1353
Size: 2311 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2644 2680 4321 4695 4841 387
Size: 1064 138 1055 36 1641 374 146 1280
Fragments arranged by size:
1641 1280 1064 1055 374 146 138 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2467 2519 2528 2775 2790 3797
Size: 393 563 272 52 9 247 15 1007
Cuts at: 3797 4394 5181 5449 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1007 787 597 563 393 272 268
247 52 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2582 2582
Size: 5734
EcoO109I rG'GnC_Cy
Cuts at: 2039 2169 2224 2269 2270 2341 2342 2355 2381
Size: 130 55 45 1 71 1 13 26
Cuts at: 2381 2774 2039
Size: 393 4999
Fragments arranged by size:
4999 393 130 71 55 45 26 13
1 1
EcoRI G'AATT_C
Cuts at: 3043 3421 3043
Size: 378 5356
FspI TGC'GCA
Cuts at: 2570 4240 2570
Size: 1670 4064
HaeII r_GCGC'y
Cuts at: 1678 4881 1678
Size: 3203 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2342 2374 2951 2983 4375
Size: 32 135 32 770 32 577 32 1392
Cuts at: 4375 4407 4449 4481 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1392 770 577 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3078 3324 669
Size: 988 281 1140 246 3079
Fragments arranged by size:
3079 1140 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5734
HpaI GTT'AAC
Cuts at: 3324 3324
Size: 5734
KpnI G_GTAC'C
Cuts at: 3059 3059
Size: 5734
MfeI C'AATT_G
Cuts at: 3333 3333
Size: 5734
MluI A'CGCG_T
Cuts at: 5610 5610
Size: 5734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4848 5032 1305
Size: 3543 184 2007
Fragments arranged by size:
3543 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2393 2444 10
Size: 54 1425 343 561 51 3300
Fragments arranged by size:
3300 1425 561 343 54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 2696 2835 2841 2861 3692 4051 4210
Size: 648 1530 139 6 20 831 359 159
Cuts at: 4210 518
Size: 2042
Fragments arranged by size:
2042 1530 831 648 359 159 139 20
6
MspA1I CmG'CkG
Cuts at: 1606 2105 2136 2143 2276 2320 2497 2560 2728
Size: 499 31 7 133 44 177 63 168
Cuts at: 2728 3150 3827 4975 5220 1606
Size: 422 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 499 422 245 177 168
133 63 44 31 7
NaeI GCC'GGC
Cuts at: 2196 2196
Size: 5734
NcoI C'CATG_G
Cuts at: 513 1065 2004 2433 2836 2905 513
Size: 552 939 429 403 69 3342
Fragments arranged by size:
3342 939 552 429 403 69
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5734
NgoMIV G'CCGG_C
Cuts at: 2194 2194
Size: 5734
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5734
NotI GC'GGCC_GC
Cuts at: 3181 3181
Size: 5734
NsiI A_TGCA'T
Cuts at: 2816 2816
Size: 5734
NspI r_CATG'y
Cuts at: 1132 1229 2818 3092 4637 1132
Size: 97 1589 274 1545 2229
Fragments arranged by size:
2229 1589 1545 274 97
PasI CC'CwG_GG
Cuts at: 1348 2923 1348
Size: 1575 4159
PciI A'CATG_T
Cuts at: 4633 4633
Size: 5734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2759 1108
Size: 1651 4083
PfoI T'CCnGG_A
Cuts at: 2655 2752 2655
Size: 97 5637
PmeI GTTT'AAAC
Cuts at: 3038 3038
Size: 5734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 5316 5348 3803
Size: 32 1481 32 4189
Fragments arranged by size:
4189 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2224 2224
Size: 5734
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5734
PsiI TTA'TAA
Cuts at: 3304 3304
Size: 5734
PspOMI G'GGCC_C
Cuts at: 2083 2168 2231 2269 2341 2354 2083
Size: 85 63 38 72 13 5463
Fragments arranged by size:
5463 85 72 63 38 13
PstI C_TGCA'G
Cuts at: 830 2223 2733 3086 830
Size: 1393 510 353 3478
Fragments arranged by size:
3478 1393 510 353
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5734
PvuII CAG'CTG
Cuts at: 2320 2497 2560 2728 2320
Size: 177 63 168 5326
Fragments arranged by size:
5326 177 168 63
SacII CC_GC'GG
Cuts at: 2137 2144 2277 2137
Size: 7 133 5594
Fragments arranged by size:
5594 133 7
SalI G'TCGA_C
Cuts at: 1936 3076 1936
Size: 1140 4594
SbfI CC_TGCA'GG
Cuts at: 2223 3086 2223
Size: 863 4871
ScaI AGT'ACT
Cuts at: 3982 5694 3982
Size: 1712 4022
SfcI C'TryA_G
Cuts at: 826 1045 2219 2729 3082 4217 4898 5089 826
Size: 219 1174 510 353 1135 681 191 1471
Fragments arranged by size:
1471 1174 1135 681 510 353 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5734
SmaI CCC'GGG
Cuts at: 1872 2418 2552 2591 3061 1872
Size: 546 134 39 470 4545
Fragments arranged by size:
4545 546 470 134 39
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3838 4739 5001 820
Size: 197 926 1895 901 262 1553
Fragments arranged by size:
1895 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3525 493
Size: 3032 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5734
SphI G_CATG'C
Cuts at: 1132 3092 1132
Size: 1960 3774
SspI AAT'ATT
Cuts at: 5 52 3658 5
Size: 47 3606 2081
Fragments arranged by size:
3606 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5734
StyI C'CwwG_G
Cuts at: 513 1065 2004 2325 2433 2836 2905 3000 3173
Size: 552 939 321 108 403 69 95 173
Cuts at: 3173 513
Size: 3074
Fragments arranged by size:
3074 939 552 403 321 173 108 95
69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3761 3778 3931 4116 4561 325
Size: 1255 2181 17 153 185 445 1498
Fragments arranged by size:
2181 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3980 5692 96
Size: 274 80 33 51 1443 2003 1712 138
Fragments arranged by size:
2003 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5694 5726 5694
Size: 32 5702
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5734
XbaI T'CTAG_A
Cuts at: 3070 3070
Size: 5734
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5734
XmaI C'CCGG_G
Cuts at: 1870 2416 2550 2589 3059 1870
Size: 546 134 39 470 4545
Fragments arranged by size:
4545 546 470 134 39
XmnI GAAnn'nnTTC
Cuts at: 2516 2672 3863 2516
Size: 156 1191 4387
Fragments arranged by size:
4387 1191 156
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3542 276
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NarI NruI PacI PmlI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI