Restriction map for
pFN21AB7679
(Circular) (Six-base) MAPSORT of: pFN21AB7679.seq Check: 1436 from: 1 to: 5731
pFN21AB7679 5731 bp
With 182 enzymes: SgfI *
March 28, 2012 12:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3541 278
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3074 1937
Size: 1137 4594
Acc65I G'GTAC_C
Cuts at: 3052 3052
Size: 5731
AclI AA'CG_TT
Cuts at: 1563 3858 4231 1563
Size: 2295 373 3063
Fragments arranged by size:
3063 2295 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3794 5178 5446 1632
Size: 2162 1384 268 1917
Fragments arranged by size:
2162 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5534
AflIII A'CryG_T
Cuts at: 1203 4630 5607 1203
Size: 3427 977 1327
Fragments arranged by size:
3427 1327 977
AgeI A'CCGG_T
Cuts at: 4562 4562
Size: 5731
AhdI GACnn_n'nnGTC
Cuts at: 4460 4460
Size: 5731
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2561 1184
Size: 1377 4354
AlwNI CAG_nnn'CTG
Cuts at: 3474 5046 3474
Size: 1572 4159
ApaI G_GGCC'C
Cuts at: 2226 2621 2226
Size: 395 5336
ApaLI G'TGCA_C
Cuts at: 2610 3788 4944 2610
Size: 1178 1156 3397
Fragments arranged by size:
3397 1178 1156
ApoI r'AATT_y
Cuts at: 1493 3040 3266 3418 5527 1493
Size: 1547 226 152 2109 1697
Fragments arranged by size:
2109 1697 1547 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4285 160
Size: 872 3253 1606
Fragments arranged by size:
3253 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 2302 2329 2336 2395 3056 1870
Size: 73 95 264 27 7 59 661 4545
Fragments arranged by size:
4545 661 264 95 73 59 27 7
BamHI G'GATC_C
Cuts at: 3061 3428 3061
Size: 367 5364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 3052 4507 618
Size: 325 217 627 278 987 1455 1842
Fragments arranged by size:
1842 1455 987 627 325 278 217
BanII G_rGCy'C
Cuts at: 1391 2226 2404 2621 3000 3050 1391
Size: 835 178 217 379 50 4072
Fragments arranged by size:
4072 835 379 217 178 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4760
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3922 3956 1903
Size: 34 1985 34 3678
Fragments arranged by size:
3678 1985 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3623 4833 3623
Size: 1210 4521
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2240 2459 4342
Size: 107 122 71 631 605 568 219 1883
Cuts at: 4342 136
Size: 1525
Fragments arranged by size:
1883 1525 631 605 568 219 122 107
71
BglII A'GATC_T
Cuts at: 5726 5726
Size: 5731
BlpI GC'TnA_GC
Cuts at: 3148 3148
Size: 5731
Bme1580I G_kGCm'C
Cuts at: 1790 2070 2226 2559 2614 2621 3792 4948 1790
Size: 280 156 333 55 7 1171 1156 2573
Fragments arranged by size:
2573 1171 1156 333 280 156 55 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2624 2842 3462
Size: 416 53 83 399 24 1207 218 620
Cuts at: 3462 4420 442
Size: 958 1753
Fragments arranged by size:
1753 1207 958 620 416 399 218 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2390 2623 4391 1239
Size: 1151 233 1768 2579
Fragments arranged by size:
2579 1768 1151 233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2156 3856 4721 5019 2156
Size: 1700 865 298 2868
Fragments arranged by size:
2868 1700 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4394 882
Size: 617 2895 2219
Fragments arranged by size:
2895 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2434 3522 493
Size: 711 1230 1088 2702
Fragments arranged by size:
2702 1230 1088 711
BsaBI GATnn'nnATC
Cuts at: 5725 5725
Size: 5731
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2322 2682 2694 3538
Size: 53 83 186 936 789 360 12 844
Cuts at: 3538 3920 4555 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 844 789 635 382 360 186
83 53 12
BsaWI w'CCGG_w
Cuts at: 2315 4164 4562 4836 4983 5394 2315
Size: 1849 398 274 147 411 2652
Fragments arranged by size:
2652 1849 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2117 2147 2739 2769 1178
Size: 30 165 30 714 30 592 30 4140
Fragments arranged by size:
4140 714 592 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 2207 2784 1186
Size: 1018 3 577 4133
Fragments arranged by size:
4133 1018 577 3
BseYI C'CCAG_C
Cuts at: 2468 2668 2999 4934 5430 2468
Size: 200 331 1935 496 2769
Fragments arranged by size:
2769 1935 496 331 200
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5158
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2250 2280 2352 2425 3181 3942
Size: 275 186 249 30 72 73 756 761
Cuts at: 3942 4970 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 756 275 249 186 73
72 30
BsiHKAI G_wGCw'C
Cuts at: 2039 2172 2614 3792 3877 4948 2039
Size: 133 442 1178 85 1071 2822
Fragments arranged by size:
2822 1178 1071 442 133 85
BsmI GAATG_Cn'
Cuts at: 3242 3335 3242
Size: 93 5638
BsmBI CGTCTCn'nnnn_
Cuts at: 2313 3018 2313
Size: 705 5026
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2070 2172 2226 2404 2559 2614
Size: 399 249 31 102 54 178 155 55
Cuts at: 2614 2621 3000 3050 3792 3877 4948 1391
Size: 7 379 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 379 249 178 155
102 85 55 54 50 31 7
BspEI T'CCGG_A
Cuts at: 2315 5394 2315
Size: 3079 2652
BspHI T'CATG_A
Cuts at: 3620 5350 3620
Size: 1730 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2541 2802 3086 844
Size: 1697 261 284 3489
Fragments arranged by size:
3489 1697 284 261
BsrBI CCG'CTC
Cuts at: 1394 1823 3618 1394
Size: 429 1795 3507
Fragments arranged by size:
3507 1795 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4226 4400 65
Size: 1183 2978 174 1396
Fragments arranged by size:
2978 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2410 4375 4562 1700
Size: 110 600 1965 187 2869
Fragments arranged by size:
2869 1965 600 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5731
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5731
BssSI C'ACGA_G
Cuts at: 3791 4803 3791
Size: 1012 4719
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2167 794
Size: 1373 4358
BstBI TT'CG_AA
Cuts at: 4535 4535
Size: 5731
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5731
BstYI r'GATC_y
Cuts at: 1972 3061 3428 3813 3830 5282 5368 5380 5726
Size: 1089 367 385 17 1452 86 12 346
Cuts at: 5726 1972
Size: 1977
Fragments arranged by size:
1977 1452 1089 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2053 2375 2457 513
Size: 552 939 49 322 82 3787
Fragments arranged by size:
3787 939 552 322 82 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2477 487
Size: 683 56 1251 3741
Fragments arranged by size:
3741 1251 683 56
BtsI GCAGTG_nn'
Cuts at: 2111 2515 2813 3247 4041 4061 2111
Size: 404 298 434 794 20 3781
Fragments arranged by size:
3781 794 434 404 298 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 3035 3035
Size: 5731
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4738 809
Size: 507 3422 1802
Fragments arranged by size:
3422 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2154 2247
Size: 54 1425 50 275 18 177 147 93
Cuts at: 2247 2277 2349 2422 3178 4067 5696 8
Size: 30 72 73 756 889 1629 43
Fragments arranged by size:
1629 1425 889 756 275 177 147 93
73 72 54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2247 2277 2349 2422 3178 1537
Size: 275 435 30 72 73 756 4090
Fragments arranged by size:
4090 756 435 275 73 72 30
EarI CTCTTCn'nnn_
Cuts at: 1353 3661 1353
Size: 2308 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2279 2510 2760 4318 4692 4838
Size: 1064 138 690 231 250 1558 374 146
Cuts at: 4838 387
Size: 1280
Fragments arranged by size:
1558 1280 1064 690 374 250 231 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2390 2623 3794 4391 5178 5446 1239
Size: 393 758 233 1171 597 787 268 1524
Fragments arranged by size:
1524 1171 787 758 597 393 268 233
EcoNI CCTnn'n_nnAGG
Cuts at: 2705 2705
Size: 5731
EcoO109I rG'GnC_Cy
Cuts at: 2222 2333 2480 2618 2222
Size: 111 147 138 5335
Fragments arranged by size:
5335 147 138 111
EcoRI G'AATT_C
Cuts at: 3040 3418 3040
Size: 378 5353
FspI TGC'GCA
Cuts at: 2024 2217 2537 4237 2024
Size: 193 320 1700 3518
Fragments arranged by size:
3518 1700 320 193
FspAI rTGC'GCAy
Cuts at: 2024 2024
Size: 5731
HaeII r_GCGC'y
Cuts at: 1678 2143 4878 1678
Size: 465 2735 2531
Fragments arranged by size:
2735 2531 465
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2972 3004 4372 4404 4446
Size: 32 135 32 1400 32 1368 32 42
Cuts at: 4446 4478 1373
Size: 32 2626
Fragments arranged by size:
2626 1400 1368 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3075 3321 669
Size: 988 281 1137 246 3079
Fragments arranged by size:
3079 1137 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5731
HpaI GTT'AAC
Cuts at: 3321 3321
Size: 5731
KpnI G_GTAC'C
Cuts at: 3056 3056
Size: 5731
MfeI C'AATT_G
Cuts at: 3330 3330
Size: 5731
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3051 4845 5029 1305
Size: 1746 1794 184 2007
Fragments arranged by size:
2007 1794 1746 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3909
Fragments arranged by size:
3909 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3689 4048 4207 518
Size: 648 2523 359 159 2042
Fragments arranged by size:
2523 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2097 2296 2377 2668 3147 3824 4972 5217
Size: 491 199 81 291 479 677 1148 245
Cuts at: 5217 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 491 479 291 245 199
81
NcoI C'CATG_G
Cuts at: 513 1065 2004 2053 513
Size: 552 939 49 4191
Fragments arranged by size:
4191 939 552 49
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 2247 2349 2422 3178 2247
Size: 102 73 756 4800
Fragments arranged by size:
4800 756 102 73
NspI r_CATG'y
Cuts at: 1132 1229 2647 2723 3089 4634 1132
Size: 97 1418 76 366 1545 2229
Fragments arranged by size:
2229 1545 1418 366 97 76
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5731
PciI A'CATG_T
Cuts at: 4630 4630
Size: 5731
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5731
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PmlI CAC'GTG
Cuts at: 2434 2434
Size: 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 5313 5345 3800
Size: 32 1481 32 4186
Fragments arranged by size:
4186 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2333 2480 2333
Size: 147 5584
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 3301 3301
Size: 5731
PspOMI G'GGCC_C
Cuts at: 2222 2617 2222
Size: 395 5336
PstI C_TGCA'G
Cuts at: 830 2706 3083 830
Size: 1876 377 3478
Fragments arranged by size:
3478 1876 377
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5731
RsrII CG'GwC_CG
Cuts at: 2550 2605 2550
Size: 55 5676
SacII CC_GC'GG
Cuts at: 2378 2378
Size: 5731
SalI G'TCGA_C
Cuts at: 1936 3073 1936
Size: 1137 4594
SanDI GG'GwC_CC
Cuts at: 2333 2480 2333
Size: 147 5584
SbfI CC_TGCA'GG
Cuts at: 3083 3083
Size: 5731
ScaI AGT'ACT
Cuts at: 3979 5691 3979
Size: 1712 4019
SfcI C'TryA_G
Cuts at: 826 1045 2702 3079 4214 4895 5086 826
Size: 219 1657 377 1135 681 191 1471
Fragments arranged by size:
1657 1471 1135 681 377 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 2304 2331 3058 1872
Size: 432 27 727 4545
Fragments arranged by size:
4545 727 432 27
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2038 2171 3835 4736 4998 820
Size: 197 926 95 133 1664 901 262 1553
Fragments arranged by size:
1664 1553 926 901 262 197 133 95
SnaBI TAC'GTA
Cuts at: 493 3522 493
Size: 3029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5731
SphI G_CATG'C
Cuts at: 1132 2647 3089 1132
Size: 1515 442 3774
Fragments arranged by size:
3774 1515 442
SspI AAT'ATT
Cuts at: 5 52 3655 5
Size: 47 3603 2081
Fragments arranged by size:
3603 2081 47
StuI AGG'CCT
Cuts at: 1517 2234 1517
Size: 717 5014
StyI C'CwwG_G
Cuts at: 513 1065 2004 2053 2740 3170 513
Size: 552 939 49 687 430 3074
Fragments arranged by size:
3074 939 687 552 430 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3758 3775 3928 4113 4558 325
Size: 1255 2178 17 153 185 445 1498
Fragments arranged by size:
2178 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3977 5689 96
Size: 274 80 33 51 1443 2000 1712 138
Fragments arranged by size:
2000 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2739 2771 2919 2951 5691 5723 2739
Size: 32 148 32 2740 32 2747
Fragments arranged by size:
2747 2740 148 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5731
XbaI T'CTAG_A
Cuts at: 3067 3067
Size: 5731
XhoI C'TCGA_G
Cuts at: 1943 2038 1943
Size: 95 5636
XmaI C'CCGG_G
Cuts at: 1870 2302 2329 3056 1870
Size: 432 27 727 4545
Fragments arranged by size:
4545 727 432 27
XmnI GAAnn'nnTTC
Cuts at: 3860 3860
Size: 5731
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3539 276
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PspXI PsrI PvuII SacI
SapI SexAI SfiI SfoI SrfI SwaI XcmI