Restriction map for
pFN21AB7675
(Circular) (Six-base) MAPSORT of: pFN21AB7675.seq Check: 7143 from: 1 to: 5728
pFN21AB7675 5728 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3538 278
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3071 1937
Size: 1134 4594
Acc65I G'GTAC_C
Cuts at: 3049 3049
Size: 5728
AclI AA'CG_TT
Cuts at: 1563 3855 4228 1563
Size: 2292 373 3063
Fragments arranged by size:
3063 2292 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3791 5175 5443 1632
Size: 2159 1384 268 1917
Fragments arranged by size:
2159 1917 1384 268
AfeI AGC'GCT
Cuts at: 2463 2463
Size: 5728
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5531
AflIII A'CryG_T
Cuts at: 1203 4627 5604 1203
Size: 3424 977 1327
Fragments arranged by size:
3424 1327 977
AgeI A'CCGG_T
Cuts at: 4559 4559
Size: 5728
AhdI GACnn_n'nnGTC
Cuts at: 4457 4457
Size: 5728
AleI CACnn'nnGTG
Cuts at: 1166 2252 2946 1166
Size: 1086 694 3948
Fragments arranged by size:
3948 1086 694
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5728
AlwNI CAG_nnn'CTG
Cuts at: 3471 5043 3471
Size: 1572 4156
ApaLI G'TGCA_C
Cuts at: 3785 4941 3785
Size: 1156 4572
ApoI r'AATT_y
Cuts at: 1493 3037 3263 3415 5524 1493
Size: 1544 226 152 2109 1697
Fragments arranged by size:
2109 1697 1544 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4282 160
Size: 872 3250 1606
Fragments arranged by size:
3250 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
AvaI C'yCGr_G
Cuts at: 1870 1943 2232 2534 3053 1870
Size: 73 289 302 519 4545
Fragments arranged by size:
4545 519 302 289 73
BamHI G'GATC_C
Cuts at: 3058 3425 3058
Size: 367 5361
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2521 2539 2776 2924 3049
Size: 325 217 627 734 18 237 148 125
Cuts at: 3049 4504 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 734 627 325 237 217 148
125 18
BanII G_rGCy'C
Cuts at: 1391 2198 2369 2749 3047 1391
Size: 807 171 380 298 4072
Fragments arranged by size:
4072 807 380 298 171
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4757
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2377 2411 3919 3953 1903
Size: 34 440 34 1508 34 3678
Fragments arranged by size:
3678 1508 440 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3620 4830 3620
Size: 1210 4518
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5728
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4339 136
Size: 107 122 71 631 605 2667 1525
Fragments arranged by size:
2667 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5723 5723
Size: 5728
BlpI GC'TnA_GC
Cuts at: 3145 3145
Size: 5728
Bme1580I G_kGCm'C
Cuts at: 1790 2358 2526 2927 3789 4945 1790
Size: 568 168 401 862 1156 2573
Fragments arranged by size:
2573 1156 862 568 401 168
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5728
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2117 3459 4417
Size: 416 53 83 399 24 700 1342 958
Cuts at: 4417 442
Size: 1753
Fragments arranged by size:
1753 1342 958 700 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5728
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 5728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2771 4388 1239
Size: 1532 1617 2579
Fragments arranged by size:
2579 1617 1532
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3853 4718 5016 3853
Size: 865 298 4565
Fragments arranged by size:
4565 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2152 2277 2792 4391 882
Size: 617 653 125 515 1599 2219
Fragments arranged by size:
2219 1599 653 617 515 125
BsaAI yAC'GTr
Cuts at: 493 1204 3519 493
Size: 711 2315 2702
Fragments arranged by size:
2702 2315 711
BsaBI GATnn'nnATC
Cuts at: 5722 5722
Size: 5728
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2450 3535 3917 4552
Size: 53 83 186 936 917 1085 382 635
Cuts at: 4552 275
Size: 1451
Fragments arranged by size:
1451 1085 936 917 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2265 4161 4559 4833 4980 5391 2265
Size: 1896 398 274 147 411 2602
Fragments arranged by size:
2602 1896 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2034 2064 1178
Size: 30 165 30 631 30 4842
Fragments arranged by size:
4842 631 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2055 2575 2687 1186
Size: 869 520 112 4227
Fragments arranged by size:
4227 869 520 112
BseYI C'CCAG_C
Cuts at: 2035 2409 2492 2791 2842 2918 4931 5427 2035
Size: 374 83 299 51 76 2013 496 2336
Fragments arranged by size:
2336 2013 496 374 299 83 76 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5155
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2120 2514 3178 3939 4967 1540
Size: 275 186 119 394 664 761 1028 2301
Fragments arranged by size:
2301 1028 761 664 394 275 186 119
BsiHKAI G_wGCw'C
Cuts at: 2369 3789 3874 4945 2369
Size: 1420 85 1071 3152
Fragments arranged by size:
3152 1420 1071 85
BsmI GAATG_Cn'
Cuts at: 2379 2985 3239 3332 2379
Size: 606 254 93 4775
Fragments arranged by size:
4775 606 254 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2183 2183
Size: 5728
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2198 2358 2369 2526 2749 2927 3047
Size: 399 408 160 11 157 223 178 120
Cuts at: 3047 3789 3874 4945 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 408 399 223 178 160
157 120 85 11
BspEI T'CCGG_A
Cuts at: 2265 5391 2265
Size: 3126 2602
BspHI T'CATG_A
Cuts at: 3617 5347 3617
Size: 1730 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3083 844
Size: 2239 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2619 3615 1394
Size: 429 796 996 3507
Fragments arranged by size:
3507 996 796 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4223 4397 65
Size: 1183 2975 174 1396
Fragments arranged by size:
2975 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4372 4559 1700
Size: 110 2562 187 2869
Fragments arranged by size:
2869 2562 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5728
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5728
BssSI C'ACGA_G
Cuts at: 3788 4800 3788
Size: 1012 4716
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5728
BstBI TT'CG_AA
Cuts at: 4532 4532
Size: 5728
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5728
BstYI r'GATC_y
Cuts at: 1972 2169 2307 3058 3425 3810 3827 5279 5365
Size: 197 138 751 367 385 17 1452 86
Cuts at: 5365 5377 5723 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 751 385 367 346 197 138
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2250 513
Size: 552 939 246 3991
Fragments arranged by size:
3991 939 552 246
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2946 487
Size: 683 56 1720 3269
Fragments arranged by size:
3269 1720 683 56
BtsI GCAGTG_nn'
Cuts at: 3244 4038 4058 3244
Size: 794 20 4914
Fragments arranged by size:
4914 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5728
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5693
DraI TTT'AAA
Cuts at: 3032 3032
Size: 5728
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4735 809
Size: 507 3419 1802
Fragments arranged by size:
3419 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2511 2562 3175
Size: 54 1425 50 275 18 681 51 613
Cuts at: 3175 4064 5693 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 681 613 275 54 51
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2511 3175 1537
Size: 275 699 664 4090
Fragments arranged by size:
4090 699 664 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3658 1353
Size: 2305 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2342 4315 4689 4835 387
Size: 1064 138 753 1973 374 146 1280
Fragments arranged by size:
1973 1280 1064 753 374 146 138
EcoICRI GAG'CTC
Cuts at: 2367 2367
Size: 5728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2771 3791 4388 5175 5443 1239
Size: 393 1139 1020 597 787 268 1524
Fragments arranged by size:
1524 1139 1020 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2753 2753
Size: 5728
EcoRI G'AATT_C
Cuts at: 3037 3415 3037
Size: 378 5350
FspI TGC'GCA
Cuts at: 2387 4234 2387
Size: 1847 3881
HaeII r_GCGC'y
Cuts at: 1678 2465 4875 1678
Size: 787 2410 2531
Fragments arranged by size:
2531 2410 787
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4369 4401 4443 4475 1373
Size: 32 135 32 2797 32 42 32 2626
Fragments arranged by size:
2797 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3072 3318 669
Size: 988 281 1134 246 3079
Fragments arranged by size:
3079 1134 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5728
HpaI GTT'AAC
Cuts at: 3318 3318
Size: 5728
KpnI G_GTAC'C
Cuts at: 3053 3053
Size: 5728
MfeI C'AATT_G
Cuts at: 3327 3327
Size: 5728
MluI A'CGCG_T
Cuts at: 5604 5604
Size: 5728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2140 2715 4842 5026 1305
Size: 835 575 2127 184 2007
Fragments arranged by size:
2127 2007 835 575 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2564 10
Size: 54 1425 343 732 3174
Fragments arranged by size:
3174 1425 732 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2252 2946 3686 4045 4204 518
Size: 648 1086 694 740 359 159 2042
Fragments arranged by size:
2042 1086 740 694 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2065 2795 3144 3821 4969 5214 1606
Size: 429 30 730 349 677 1148 245 2120
Fragments arranged by size:
2120 1148 730 677 429 349 245 30
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4237
Fragments arranged by size:
4237 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5728
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5728
NotI GC'GGCC_GC
Cuts at: 2511 3175 2511
Size: 664 5064
NspI r_CATG'y
Cuts at: 1132 1229 2723 2918 3086 4631 1132
Size: 97 1494 195 168 1545 2229
Fragments arranged by size:
2229 1545 1494 195 168 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5728
PciI A'CATG_T
Cuts at: 4627 4627
Size: 5728
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5728
PfoI T'CCnGG_A
Cuts at: 2310 2310
Size: 5728
PmeI GTTT'AAAC
Cuts at: 3032 3032
Size: 5728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3797 3829 5310 5342 3797
Size: 32 1481 32 4183
Fragments arranged by size:
4183 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5728
PsiI TTA'TAA
Cuts at: 3298 3298
Size: 5728
PstI C_TGCA'G
Cuts at: 830 3080 830
Size: 2250 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5728
PvuII CAG'CTG
Cuts at: 2035 2795 2035
Size: 760 4968
SacI G_AGCT'C
Cuts at: 2369 2369
Size: 5728
SalI G'TCGA_C
Cuts at: 1936 3070 1936
Size: 1134 4594
SbfI CC_TGCA'GG
Cuts at: 3080 3080
Size: 5728
ScaI AGT'ACT
Cuts at: 3976 5688 3976
Size: 1712 4016
SfcI C'TryA_G
Cuts at: 826 1045 2869 3076 4211 4892 5083 826
Size: 219 1824 207 1135 681 191 1471
Fragments arranged by size:
1824 1471 1135 681 219 207 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5728
SmaI CCC'GGG
Cuts at: 1872 2234 2536 3055 1872
Size: 362 302 519 4545
Fragments arranged by size:
4545 519 362 302
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3832 4733 4995 820
Size: 197 926 1889 901 262 1553
Fragments arranged by size:
1889 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3519 493
Size: 3026 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5728
SphI G_CATG'C
Cuts at: 1132 2723 3086 1132
Size: 1591 363 3774
Fragments arranged by size:
3774 1591 363
SspI AAT'ATT
Cuts at: 5 52 3652 5
Size: 47 3600 2081
Fragments arranged by size:
3600 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5728
StyI C'CwwG_G
Cuts at: 513 1065 2004 2517 2664 3167 513
Size: 552 939 513 147 503 3074
Fragments arranged by size:
3074 939 552 513 503 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2895 3755 3772 3925 4110 4555 325
Size: 1255 1315 860 17 153 185 445 1498
Fragments arranged by size:
1498 1315 1255 860 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2346 3974 5686
Size: 274 80 33 51 1443 369 1628 1712
Cuts at: 5686 96
Size: 138
Fragments arranged by size:
1712 1628 1443 369 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2034 2066 2321 2353 5688 5720 2034
Size: 32 255 32 3335 32 2042
Fragments arranged by size:
3335 2042 255 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5728
XbaI T'CTAG_A
Cuts at: 3064 3064
Size: 5728
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5728
XmaI C'CCGG_G
Cuts at: 1870 2232 2534 3053 1870
Size: 362 302 519 4545
Fragments arranged by size:
4545 519 362 302
XmnI GAAnn'nnTTC
Cuts at: 3857 3857
Size: 5728
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3536 276
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI