Restriction map for
pFN21AB7673
(Circular) (Six-base) MAPSORT of: pFN21AB7673.seq Check: 2468 from: 1 to: 5725
pFN21AB7673 5725 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2254 2254
Size: 5725
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3535 278
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3068 1937
Size: 1131 4594
Acc65I G'GTAC_C
Cuts at: 3046 3046
Size: 5725
AclI AA'CG_TT
Cuts at: 1563 3852 4225 1563
Size: 2289 373 3063
Fragments arranged by size:
3063 2289 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2724 3788 5172 5440 1632
Size: 1092 1064 1384 268 1917
Fragments arranged by size:
1917 1384 1092 1064 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 4624 5601 1203
Size: 3421 977 1327
Fragments arranged by size:
3421 1327 977
AgeI A'CCGG_T
Cuts at: 2012 4556 2012
Size: 2544 3181
AhdI GACnn_n'nnGTC
Cuts at: 4454 4454
Size: 5725
AleI CACnn'nnGTG
Cuts at: 1166 2495 1166
Size: 1329 4396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5725
AlwNI CAG_nnn'CTG
Cuts at: 3468 5040 3468
Size: 1572 4153
ApaI G_GGCC'C
Cuts at: 2120 2429 2120
Size: 309 5416
ApaLI G'TGCA_C
Cuts at: 3782 4938 3782
Size: 1156 4569
ApoI r'AATT_y
Cuts at: 1493 3034 3260 3412 5521 1493
Size: 1541 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 2113 2128 2224 2261 2422 3050 1870
Size: 73 170 15 96 37 161 628 4545
Fragments arranged by size:
4545 628 170 161 96 73 37 15
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2775 2808 2775
Size: 33 5692
BamHI G'GATC_C
Cuts at: 3055 3422 3055
Size: 367 5358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2180 3046 4501 618
Size: 325 217 627 393 866 1455 1842
Fragments arranged by size:
1842 1455 866 627 393 325 217
BanII G_rGCy'C
Cuts at: 1391 2120 2176 2211 2340 2423 2429 3044 1391
Size: 729 56 35 129 83 6 615 4072
Fragments arranged by size:
4072 729 615 129 83 56 35 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4754
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2020 2054 3916 3950 1903
Size: 34 83 34 1862 34 3678
Fragments arranged by size:
3678 1862 83 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3617 4827 3617
Size: 1210 4515
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2043 4336 136
Size: 107 122 71 631 605 371 2293 1525
Fragments arranged by size:
2293 1525 631 605 371 122 107 71
BglII A'GATC_T
Cuts at: 2824 5720 2824
Size: 2896 2829
BlpI GC'TnA_GC
Cuts at: 3142 3142
Size: 5725
Bme1580I G_kGCm'C
Cuts at: 1790 2120 2183 2429 3786 4942 1790
Size: 330 63 246 1357 1156 2573
Fragments arranged by size:
2573 1357 1156 330 246 63
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2424 3456 4414
Size: 416 53 83 399 24 1007 1032 958
Cuts at: 4414 442
Size: 1753
Fragments arranged by size:
1753 1032 1007 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 4385 1239
Size: 845 2301 2579
Fragments arranged by size:
2579 2301 845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2701 3850 4715 5013 2701
Size: 1149 865 298 3413
Fragments arranged by size:
3413 1149 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2934 4388 882
Size: 617 1435 1454 2219
Fragments arranged by size:
2219 1454 1435 617
BsaAI yAC'GTr
Cuts at: 493 1204 3516 493
Size: 711 2312 2702
Fragments arranged by size:
2702 2312 711
BsaBI GATnn'nnATC
Cuts at: 5719 5719
Size: 5725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2148 2400 3532 3914
Size: 53 83 186 936 615 252 1132 382
Cuts at: 3914 4549 275
Size: 635 1451
Fragments arranged by size:
1451 1132 936 635 615 382 252 186
83 53
BsaWI w'CCGG_w
Cuts at: 2012 2022 4158 4556 4830 4977 5388 2012
Size: 10 2136 398 274 147 411 2349
Fragments arranged by size:
2349 2136 411 398 274 147 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5500
Fragments arranged by size:
5500 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2399 2402 1186
Size: 895 318 3 4509
Fragments arranged by size:
4509 895 318 3
BseYI C'CCAG_C
Cuts at: 2153 2341 2373 4928 5424 2153
Size: 188 32 2555 496 2454
Fragments arranged by size:
2555 2454 496 188 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2346 1163
Size: 573 610 4542
Fragments arranged by size:
4542 610 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 2193 2283 2327 3175 3936
Size: 275 186 12 180 90 44 848 761
Cuts at: 3936 4964 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 848 761 275 186 180 90
44 12
BsiHKAI G_wGCw'C
Cuts at: 2211 2423 3786 3871 4942 2211
Size: 212 1363 85 1071 2994
Fragments arranged by size:
2994 1363 1071 212 85
BsmI GAATG_Cn'
Cuts at: 3236 3329 3236
Size: 93 5632
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 2176 2183 2211 2340 2423 2429
Size: 399 330 56 7 28 129 83 6
Cuts at: 2429 3044 3786 3871 4942 1391
Size: 615 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 615 399 330 129 85
83 56 28 7 6
BspEI T'CCGG_A
Cuts at: 5388 5388
Size: 5725
BspHI T'CATG_A
Cuts at: 3614 5344 3614
Size: 1730 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2254 3080 844
Size: 1410 826 3489
Fragments arranged by size:
3489 1410 826
BsrBI CCG'CTC
Cuts at: 1394 1823 2563 3612 1394
Size: 429 740 1049 3507
Fragments arranged by size:
3507 1049 740 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4220 4394 65
Size: 1183 2972 174 1396
Fragments arranged by size:
2972 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2012 2492 4369 4556 1700
Size: 110 202 480 1877 187 2869
Fragments arranged by size:
2869 1877 480 202 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5725
BssHII G'CGCG_C
Cuts at: 1925 2095 2232 1925
Size: 170 137 5418
Fragments arranged by size:
5418 170 137
BssSI C'ACGA_G
Cuts at: 3785 4797 3785
Size: 1012 4713
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5725
BstBI TT'CG_AA
Cuts at: 4529 4529
Size: 5725
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5725
BstXI CCAn_nnnn'nTGG
Cuts at: 2497 2497
Size: 5725
BstYI r'GATC_y
Cuts at: 1972 2626 2824 3055 3422 3807 3824 5276 5362
Size: 654 198 231 367 385 17 1452 86
Cuts at: 5362 5374 5720 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 654 385 367 346 231 198
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2166 2318 513
Size: 552 939 162 152 3920
Fragments arranged by size:
3920 939 552 162 152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4986
Fragments arranged by size:
4986 683 56
BtsI GCAGTG_nn'
Cuts at: 3241 4035 4055 3241
Size: 794 20 4911
Fragments arranged by size:
4911 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5690
DraI TTT'AAA
Cuts at: 3029 3029
Size: 5725
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4732 809
Size: 507 3416 1802
Fragments arranged by size:
3416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 3172 4061
Size: 54 1425 50 275 18 360 982 889
Cuts at: 4061 5690 8
Size: 1629 43
Fragments arranged by size:
1629 1425 982 889 360 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2190 3172 1537
Size: 275 378 982 4090
Fragments arranged by size:
4090 982 378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2456 3655 1353
Size: 1103 1199 3423
Fragments arranged by size:
3423 1199 1103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2278 4312 4686 4832 387
Size: 1064 138 689 2034 374 146 1280
Fragments arranged by size:
2034 1280 1064 689 374 146 138
EcoICRI GAG'CTC
Cuts at: 2209 2421 2209
Size: 212 5513
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2724 3788 4385 5172 5440 1239
Size: 393 452 640 1064 597 787 268 1524
Fragments arranged by size:
1524 1064 787 640 597 452 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2426 2426
Size: 5725
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
FspI TGC'GCA
Cuts at: 4231 4231
Size: 5725
HaeII r_GCGC'y
Cuts at: 1678 2488 4872 1678
Size: 810 2384 2531
Fragments arranged by size:
2531 2384 810
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4366 4398 4440 4472 1373
Size: 32 135 32 2794 32 42 32 2626
Fragments arranged by size:
2794 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2503 3069 3315 669
Size: 988 281 565 566 246 3079
Fragments arranged by size:
3079 988 566 565 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5725
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KpnI G_GTAC'C
Cuts at: 3050 3050
Size: 5725
MfeI C'AATT_G
Cuts at: 3324 3324
Size: 5725
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2307 4839 5023 1305
Size: 1002 2532 184 2007
Fragments arranged by size:
2532 2007 1002 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3903
Fragments arranged by size:
3903 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 3683 4042 4201 518
Size: 648 1329 1188 359 159 2042
Fragments arranged by size:
2042 1329 1188 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2034 2153 2168 2198 2320 3016 3141 3818
Size: 428 119 15 30 122 696 125 677
Cuts at: 3818 4966 5211 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 696 677 428 245 125 122
119 30 15
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4234
Fragments arranged by size:
4234 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 2190 3172 2190
Size: 982 4743
NspI r_CATG'y
Cuts at: 1132 1229 3083 4628 1132
Size: 97 1854 1545 2229
Fragments arranged by size:
2229 1854 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5725
PciI A'CATG_T
Cuts at: 4624 4624
Size: 5725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5725
PfoI T'CCnGG_A
Cuts at: 2061 2061
Size: 5725
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 5307 5339 3794
Size: 32 1481 32 4180
Fragments arranged by size:
4180 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5725
PsiI TTA'TAA
Cuts at: 3295 3295
Size: 5725
PspOMI G'GGCC_C
Cuts at: 2116 2425 2116
Size: 309 5416
PstI C_TGCA'G
Cuts at: 830 3077 830
Size: 2247 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
PvuII CAG'CTG
Cuts at: 3016 3016
Size: 5725
SacI G_AGCT'C
Cuts at: 2211 2423 2211
Size: 212 5513
SacII CC_GC'GG
Cuts at: 2169 2321 2169
Size: 152 5573
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SbfI CC_TGCA'GG
Cuts at: 3077 3077
Size: 5725
ScaI AGT'ACT
Cuts at: 3973 5685 3973
Size: 1712 4013
SfcI C'TryA_G
Cuts at: 826 1045 2648 3073 4208 4889 5080 826
Size: 219 1603 425 1135 681 191 1471
Fragments arranged by size:
1603 1471 1135 681 425 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 2492 1700
Size: 792 4933
SmaI CCC'GGG
Cuts at: 1872 2115 2130 2226 2263 3052 1872
Size: 243 15 96 37 789 4545
Fragments arranged by size:
4545 789 243 96 37 15
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2716 3829 4730 4992 820
Size: 197 926 773 1113 901 262 1553
Fragments arranged by size:
1553 1113 926 901 773 262 197
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 3083 1132
Size: 1951 3774
SspI AAT'ATT
Cuts at: 5 52 3649 5
Size: 47 3597 2081
Fragments arranged by size:
3597 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5725
StyI C'CwwG_G
Cuts at: 513 1065 2004 3164 513
Size: 552 939 1160 3074
Fragments arranged by size:
3074 1160 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3752 3769 3922 4107 4552 325
Size: 1255 2172 17 153 185 445 1498
Fragments arranged by size:
2172 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3971 5683 96
Size: 274 80 33 51 1443 1994 1712 138
Fragments arranged by size:
1994 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2235 2267 5685 5717 2235
Size: 32 3418 32 2243
Fragments arranged by size:
3418 2243 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2399 1316
Size: 1083 4642
XbaI T'CTAG_A
Cuts at: 3061 3061
Size: 5725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2059 2059
Size: 5725
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5725
XmaI C'CCGG_G
Cuts at: 1870 2113 2128 2224 2261 3050 1870
Size: 243 15 96 37 789 4545
Fragments arranged by size:
4545 789 243 96 37 15
XmnI GAAnn'nnTTC
Cuts at: 2668 3854 2668
Size: 1186 4539
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3533 276
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI
SapI SexAI SfiI SfoI SrfI SwaI