Restriction map for
pFN21AB7671
(Circular) (Six-base) MAPSORT of: pFN21AB7671.seq Check: 5282 from: 1 to: 5719
pFN21AB7671 5719 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2518 2575 2667 2518
Size: 57 92 5570
Fragments arranged by size:
5570 92 57
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3529 278
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3062 1937
Size: 1125 4594
Acc65I G'GTAC_C
Cuts at: 3040 3040
Size: 5719
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3782 5166 5434 1632
Size: 2150 1384 268 1917
Fragments arranged by size:
2150 1917 1384 268
AfeI AGC'GCT
Cuts at: 2348 2501 2348
Size: 153 5566
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5522
AflIII A'CryG_T
Cuts at: 1203 2550 4618 5595 1203
Size: 1347 2068 977 1327
Fragments arranged by size:
2068 1347 1327 977
AgeI A'CCGG_T
Cuts at: 4550 4550
Size: 5719
AhdI GACnn_n'nnGTC
Cuts at: 4448 4448
Size: 5719
AleI CACnn'nnGTG
Cuts at: 1166 2819 1166
Size: 1653 4066
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5719
AlwNI CAG_nnn'CTG
Cuts at: 2362 2488 2659 2674 2866 3462 5034 2362
Size: 126 171 15 192 596 1572 3047
Fragments arranged by size:
3047 1572 596 192 171 126 15
ApaI G_GGCC'C
Cuts at: 2768 2768
Size: 5719
ApaLI G'TGCA_C
Cuts at: 3776 4932 3776
Size: 1156 4563
ApoI r'AATT_y
Cuts at: 1493 3028 3254 3406 5515 1493
Size: 1535 226 152 2109 1697
Fragments arranged by size:
2109 1697 1535 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4273 160
Size: 872 3241 1606
Fragments arranged by size:
3241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 3044 1870
Size: 73 174 927 4545
Fragments arranged by size:
4545 927 174 73
BamHI G'GATC_C
Cuts at: 3049 3416 3049
Size: 367 5352
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 2035 2846 3040 4495
Size: 325 217 627 233 15 811 194 1455
Cuts at: 4495 618
Size: 1842
Fragments arranged by size:
1842 1455 811 627 325 233 217 194
15
BanII G_rGCy'C
Cuts at: 1391 2088 2768 3038 1391
Size: 697 680 270 4072
Fragments arranged by size:
4072 697 680 270
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2318 928
Size: 971 419 4329
Fragments arranged by size:
4329 971 419
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3910 3944 1903
Size: 34 1973 34 3678
Fragments arranged by size:
3678 1973 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3611 4821 3611
Size: 1210 4509
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4330 136
Size: 107 122 71 631 605 2658 1525
Fragments arranged by size:
2658 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5714 5714
Size: 5719
BlpI GC'TnA_GC
Cuts at: 2252 2363 3136 2252
Size: 111 773 4835
Fragments arranged by size:
4835 773 111
Bme1580I G_kGCm'C
Cuts at: 1790 2025 2040 2768 2849 3780 4936 1790
Size: 235 15 728 81 931 1156 2573
Fragments arranged by size:
2573 1156 931 728 235 81 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5719
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3450 4408 442
Size: 416 53 83 399 24 2033 958 1753
Fragments arranged by size:
2033 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5719
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2267 2267
Size: 5719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2255 2321 2540 2561 2660 2888 4379 1239
Size: 1016 66 219 21 99 228 1491 2579
Fragments arranged by size:
2579 1491 1016 228 219 99 66 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2201 2327 2552 3844 4709 5007 2201
Size: 126 225 1292 865 298 2913
Fragments arranged by size:
2913 1292 865 298 225 126
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4382 882
Size: 617 2883 2219
Fragments arranged by size:
2883 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3510 493
Size: 711 2306 2702
Fragments arranged by size:
2702 2306 711
BsaBI GATnn'nnATC
Cuts at: 5713 5713
Size: 5719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2970 3526 3908 4543
Size: 53 83 186 936 1437 556 382 635
Cuts at: 4543 275
Size: 1451
Fragments arranged by size:
1451 1437 936 635 556 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4152 4550 4824 4971 5382 4152
Size: 398 274 147 411 4489
Fragments arranged by size:
4489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2279 2309 2927 2957 2981
Size: 30 165 30 876 30 618 30 24
Cuts at: 2981 3011 1178
Size: 30 3886
Fragments arranged by size:
3886 876 618 165 30 30 30 30
30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2537 2718 2721 2761 2885 2888 3005 1186
Size: 1351 181 3 40 124 3 117 3900
Fragments arranged by size:
3900 1351 181 124 117 40 3 3
BseYI C'CCAG_C
Cuts at: 2667 4922 5418 2667
Size: 2255 496 2968
Fragments arranged by size:
2968 2255 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2609 2843 1163
Size: 573 873 234 4039
Fragments arranged by size:
4039 873 573 234
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3169 3930 4958 1540
Size: 275 186 1168 761 1028 2301
Fragments arranged by size:
2301 1168 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3780 3865 4936 3780
Size: 85 1071 4563
Fragments arranged by size:
4563 1071 85
BsmI GAATG_Cn'
Cuts at: 3230 3323 3230
Size: 93 5626
BsmBI CGTCTCn'nnnn_
Cuts at: 2173 2173
Size: 5719
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2040 2088 2768 2849 3038 3780
Size: 399 235 15 48 680 81 189 742
Cuts at: 3780 3865 4936 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 680 399 235 189 85
81 48 15
BspEI T'CCGG_A
Cuts at: 5382 5382
Size: 5719
BspHI T'CATG_A
Cuts at: 3608 5338 3608
Size: 1730 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2463 2518 2575 2667 3074 844
Size: 1619 55 57 92 407 3489
Fragments arranged by size:
3489 1619 407 92 57 55
BsrBI CCG'CTC
Cuts at: 1394 1823 2140 2176 2367 2644 3606 1394
Size: 429 317 36 191 277 962 3507
Fragments arranged by size:
3507 962 429 317 277 191 36
BsrDI GCAATG_nn'
Cuts at: 65 1248 4214 4388 65
Size: 1183 2966 174 1396
Fragments arranged by size:
2966 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4363 4550 1700
Size: 110 2553 187 2869
Fragments arranged by size:
2869 2553 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5719
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5719
BssSI C'ACGA_G
Cuts at: 3779 4791 3779
Size: 1012 4707
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2587 2659 794
Size: 1793 72 3854
Fragments arranged by size:
3854 1793 72
BstBI TT'CG_AA
Cuts at: 4523 4523
Size: 5719
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5719
BstYI r'GATC_y
Cuts at: 1972 3049 3416 3801 3818 5270 5356 5368 5714
Size: 1077 367 385 17 1452 86 12 346
Cuts at: 5714 1972
Size: 1977
Fragments arranged by size:
1977 1452 1077 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2703 2760 2973 513
Size: 552 939 699 57 213 3259
Fragments arranged by size:
3259 939 699 552 213 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4980
Fragments arranged by size:
4980 683 56
BtsI GCAGTG_nn'
Cuts at: 3235 4029 4049 3235
Size: 794 20 4905
Fragments arranged by size:
4905 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 3023 3023
Size: 5719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4726 809
Size: 507 3410 1802
Fragments arranged by size:
3410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3166 4055 5684
Size: 54 1425 50 275 18 1336 889 1629
Cuts at: 5684 8
Size: 43
Fragments arranged by size:
1629 1425 1336 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3166 1537
Size: 275 1354 4090
Fragments arranged by size:
4090 1354 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3649 1353
Size: 2296 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2158 2462 4306 4680 4826 387
Size: 1064 138 569 304 1844 374 146 1280
Fragments arranged by size:
1844 1280 1064 569 374 304 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2321 2540 2561 2660 2888 3782
Size: 393 623 66 219 21 99 228 894
Cuts at: 3782 4379 5166 5434 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 894 787 623 597 393 268 228
219 99 66 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2303 2303
Size: 5719
EcoO109I rG'GnC_Cy
Cuts at: 2452 2765 2923 2452
Size: 313 158 5248
Fragments arranged by size:
5248 313 158
EcoRI G'AATT_C
Cuts at: 3028 3406 3028
Size: 378 5341
FspI TGC'GCA
Cuts at: 2510 2580 4225 2510
Size: 70 1645 4004
Fragments arranged by size:
4004 1645 70
FspAI rTGC'GCAy
Cuts at: 2510 2510
Size: 5719
HaeII r_GCGC'y
Cuts at: 1678 2350 2503 4866 1678
Size: 672 153 2363 2531
Fragments arranged by size:
2531 2363 672 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2877 2909 2927 2959 2981
Size: 32 135 32 1305 32 18 32 22
Cuts at: 2981 3013 4360 4392 4434 4466 1373
Size: 32 1347 32 42 32 2626
Fragments arranged by size:
2626 1347 1305 135 42 32 32 32
32 32 32 32 22 18
HincII GTy'rAC
Cuts at: 669 1657 1938 3063 3309 669
Size: 988 281 1125 246 3079
Fragments arranged by size:
3079 1125 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5719
HpaI GTT'AAC
Cuts at: 3309 3309
Size: 5719
KpnI G_GTAC'C
Cuts at: 3044 3044
Size: 5719
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2970 4833 5017 1305
Size: 1665 1863 184 2007
Fragments arranged by size:
2007 1863 1665 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3897
Fragments arranged by size:
3897 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2748 2819 3677 4036 4195 518
Size: 648 1582 71 858 359 159 2042
Fragments arranged by size:
2042 1582 858 648 359 159 71
MspA1I CmG'CkG
Cuts at: 1606 2128 2488 2593 2881 2913 3135 3812 4960
Size: 522 360 105 288 32 222 677 1148
Cuts at: 4960 5205 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 522 360 288 245 222
105 32
NcoI C'CATG_G
Cuts at: 513 1065 2004 2760 2973 513
Size: 552 939 756 213 3259
Fragments arranged by size:
3259 939 756 552 213
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5719
NotI GC'GGCC_GC
Cuts at: 3166 3166
Size: 5719
NspI r_CATG'y
Cuts at: 1132 1229 2188 2554 3077 4622 1132
Size: 97 959 366 523 1545 2229
Fragments arranged by size:
2229 1545 959 523 366 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5719
PciI A'CATG_T
Cuts at: 2550 4618 2550
Size: 2068 3651
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5719
PfoI T'CCnGG_A
Cuts at: 2355 2355
Size: 5719
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 5301 5333 3788
Size: 32 1481 32 4174
Fragments arranged by size:
4174 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2452 2452
Size: 5719
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5719
PsiI TTA'TAA
Cuts at: 3289 3289
Size: 5719
PspOMI G'GGCC_C
Cuts at: 2764 2764
Size: 5719
PstI C_TGCA'G
Cuts at: 830 2598 3071 830
Size: 1768 473 3478
Fragments arranged by size:
3478 1768 473
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
PvuII CAG'CTG
Cuts at: 2488 2593 2913 2488
Size: 105 320 5294
Fragments arranged by size:
5294 320 105
SalI G'TCGA_C
Cuts at: 1936 3061 1936
Size: 1125 4594
SbfI CC_TGCA'GG
Cuts at: 3071 3071
Size: 5719
ScaI AGT'ACT
Cuts at: 3967 5679 3967
Size: 1712 4007
SfcI C'TryA_G
Cuts at: 826 1045 2089 2594 3067 4202 4883 5074 826
Size: 219 1044 505 473 1135 681 191 1471
Fragments arranged by size:
1471 1135 1044 681 505 473 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5719
SmaI CCC'GGG
Cuts at: 1872 3046 1872
Size: 1174 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2216 2342 2567 3823 4724 4986
Size: 197 926 273 126 225 1256 901 262
Cuts at: 4986 820
Size: 1553
Fragments arranged by size:
1553 1256 926 901 273 262 225 197
126
SnaBI TAC'GTA
Cuts at: 493 3510 493
Size: 3017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 2188 3077 1132
Size: 1056 889 3774
Fragments arranged by size:
3774 1056 889
SspI AAT'ATT
Cuts at: 5 52 3643 5
Size: 47 3591 2081
Fragments arranged by size:
3591 2081 47
StuI AGG'CCT
Cuts at: 1517 2957 1517
Size: 1440 4279
StyI C'CwwG_G
Cuts at: 513 1065 2004 2760 2973 3158 513
Size: 552 939 756 213 185 3074
Fragments arranged by size:
3074 939 756 552 213 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2708 2764 2864 3746 3763 3916 4101
Size: 1255 1128 56 100 882 17 153 185
Cuts at: 4101 4546 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1128 882 445 185 153 100
56 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2286 3965 5677
Size: 274 80 33 51 1443 309 1679 1712
Cuts at: 5677 96
Size: 138
Fragments arranged by size:
1712 1679 1443 309 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5679 5711 5679
Size: 32 5687
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5719
XbaI T'CTAG_A
Cuts at: 3055 3055
Size: 5719
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5719
XmaI C'CCGG_G
Cuts at: 1870 3044 1870
Size: 1174 4545
XmnI GAAnn'nnTTC
Cuts at: 3848 3848
Size: 5719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3527 276
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI