Restriction map for
pFN21AB7670
(Circular) (Six-base) MAPSORT of: pFN21AB7670.seq Check: 5786 from: 1 to: 5890
pFN21AB7670 5890 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3700 278
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3233 1937
Size: 1296 4594
Acc65I G'GTAC_C
Cuts at: 3211 3211
Size: 5890
AclI AA'CG_TT
Cuts at: 1563 4017 4390 1563
Size: 2454 373 3063
Fragments arranged by size:
3063 2454 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3953 5337 5605 1632
Size: 2321 1384 268 1917
Fragments arranged by size:
2321 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5693
AflIII A'CryG_T
Cuts at: 1203 4789 5766 1203
Size: 3586 977 1327
Fragments arranged by size:
3586 1327 977
AgeI A'CCGG_T
Cuts at: 4721 4721
Size: 5890
AhdI GACnn_n'nnGTC
Cuts at: 4619 4619
Size: 5890
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5890
AlwNI CAG_nnn'CTG
Cuts at: 2355 2494 2881 3093 3633 5205 2355
Size: 139 387 212 540 1572 3040
Fragments arranged by size:
3040 1572 540 387 212 139
ApaI G_GGCC'C
Cuts at: 2121 2121
Size: 5890
ApaLI G'TGCA_C
Cuts at: 3947 5103 3947
Size: 1156 4734
ApoI r'AATT_y
Cuts at: 1493 2468 3199 3425 3577 5686 1493
Size: 975 731 226 152 2109 1697
Fragments arranged by size:
2109 1697 975 731 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4444 160
Size: 872 3412 1606
Fragments arranged by size:
3412 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 2114 3215 1870
Size: 73 81 90 1101 4545
Fragments arranged by size:
4545 1101 90 81 73
BamHI G'GATC_C
Cuts at: 3220 3587 3220
Size: 367 5523
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2844 3211 4666 618
Size: 325 217 627 1057 367 1455 1842
Fragments arranged by size:
1842 1455 1057 627 367 325 217
BanII G_rGCy'C
Cuts at: 1391 2121 2147 2546 2981 3122 3209 1391
Size: 730 26 399 435 141 87 4072
Fragments arranged by size:
4072 730 435 399 141 87 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4919
BbvCI CC'TCA_GC
Cuts at: 3086 3086
Size: 5890
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4081 4115 1903
Size: 34 2144 34 3678
Fragments arranged by size:
3678 2144 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3782 4992 3782
Size: 1210 4680
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5890
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2708 2985 4501
Size: 107 122 71 631 605 1036 277 1516
Cuts at: 4501 136
Size: 1525
Fragments arranged by size:
1525 1516 1036 631 605 277 122 107
71
BglII A'GATC_T
Cuts at: 5885 5885
Size: 5890
BlpI GC'TnA_GC
Cuts at: 2531 3307 2531
Size: 776 5114
Bme1580I G_kGCm'C
Cuts at: 1790 2121 3951 5107 1790
Size: 331 1830 1156 2573
Fragments arranged by size:
2573 1830 1156 331
BmgBI CAC'GTC
Cuts at: 1318 3115 1318
Size: 1797 4093
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3621 4579 442
Size: 416 53 83 399 24 2204 958 1753
Fragments arranged by size:
2204 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5890
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2575 2575
Size: 5890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2420 4550 1239
Size: 815 366 2130 2579
Fragments arranged by size:
2579 2130 815 366
Bpu10I CC'TnA_GC
Cuts at: 3086 3086
Size: 5890
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2462 4015 4880 5178 2462
Size: 1553 865 298 3174
Fragments arranged by size:
3174 1553 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2728 4553 882
Size: 617 1229 1825 2219
Fragments arranged by size:
2219 1825 1229 617
BsaAI yAC'GTr
Cuts at: 493 1204 3681 493
Size: 711 2477 2702
Fragments arranged by size:
2702 2477 711
BsaBI GATnn'nnATC
Cuts at: 5884 5884
Size: 5890
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3697 4079 4714 275
Size: 53 83 186 936 2164 382 635 1451
Fragments arranged by size:
2164 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4323 4721 4995 5142 5553 4323
Size: 398 274 147 411 4660
Fragments arranged by size:
4660 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2393 2423 2882 2912 3104
Size: 30 165 30 990 30 459 30 192
Cuts at: 3104 3134 3170 3200 1178
Size: 30 36 30 3868
Fragments arranged by size:
3868 990 459 192 165 36 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 2252 3097 1186
Size: 925 141 845 3979
Fragments arranged by size:
3979 925 845 141
BseYI C'CCAG_C
Cuts at: 2140 2874 2881 5093 5589 2140
Size: 734 7 2212 496 2441
Fragments arranged by size:
2441 2212 734 496 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2368 1163
Size: 573 632 4685
Fragments arranged by size:
4685 632 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2200 3154 3340 4101 5129 1540
Size: 275 186 199 954 186 761 1028 2301
Fragments arranged by size:
2301 1028 954 761 275 199 186 186
BsiHKAI G_wGCw'C
Cuts at: 2103 2478 3951 4036 5107 2103
Size: 375 1473 85 1071 2886
Fragments arranged by size:
2886 1473 1071 375 85
BsmI GAATG_Cn'
Cuts at: 3401 3494 3401
Size: 93 5797
BsmBI CGTCTCn'nnnn_
Cuts at: 2762 2762
Size: 5890
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2121 2147 2478 2546 2981 3122
Size: 399 313 18 26 331 68 435 141
Cuts at: 3122 3209 3951 4036 5107 1391
Size: 87 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 435 399 331 313 141
87 85 68 26 18
BspEI T'CCGG_A
Cuts at: 5553 5553
Size: 5890
BspHI T'CATG_A
Cuts at: 3779 5509 3779
Size: 1730 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2535 3245 844
Size: 1691 710 3489
Fragments arranged by size:
3489 1691 710
BsrBI CCG'CTC
Cuts at: 1394 1823 2446 3777 1394
Size: 429 623 1331 3507
Fragments arranged by size:
3507 1331 623 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4385 4559 65
Size: 1183 3137 174 1396
Fragments arranged by size:
3137 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2596 3153 4534 4721 1700
Size: 110 786 557 1381 187 2869
Fragments arranged by size:
2869 1381 786 557 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5890
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5890
BssSI C'ACGA_G
Cuts at: 2234 3950 4962 2234
Size: 1716 1012 3162
Fragments arranged by size:
3162 1716 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2107 2355 794
Size: 1313 248 4329
Fragments arranged by size:
4329 1313 248
BstBI TT'CG_AA
Cuts at: 4694 4694
Size: 5890
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5890
BstXI CCAn_nnnn'nTGG
Cuts at: 2077 2908 2077
Size: 831 5059
BstYI r'GATC_y
Cuts at: 1972 3220 3587 3972 3989 5441 5527 5539 5885
Size: 1248 367 385 17 1452 86 12 346
Cuts at: 5885 1972
Size: 1977
Fragments arranged by size:
1977 1452 1248 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2651 2967 513
Size: 552 939 647 316 3436
Fragments arranged by size:
3436 939 647 552 316
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5151
Fragments arranged by size:
5151 683 56
BtsI GCAGTG_nn'
Cuts at: 2255 3406 4200 4220 2255
Size: 1151 794 20 3925
Fragments arranged by size:
3925 1151 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5855
DraI TTT'AAA
Cuts at: 3194 3194
Size: 5890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4897 809
Size: 507 3581 1802
Fragments arranged by size:
3581 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2340 2598 2964
Size: 54 1425 50 275 18 510 258 366
Cuts at: 2964 3337 4226 5855 8
Size: 373 889 1629 43
Fragments arranged by size:
1629 1425 889 510 373 366 275 258
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3337 1537
Size: 275 1525 4090
Fragments arranged by size:
4090 1525 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2339 2561 2692 3820 1353
Size: 986 222 131 1128 3423
Fragments arranged by size:
3423 1128 986 222 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4477 4851 4997 387
Size: 1064 138 2888 374 146 1280
Fragments arranged by size:
2888 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2420 3953 4550 5337 5605 1239
Size: 393 422 366 1533 597 787 268 1524
Fragments arranged by size:
1533 1524 787 597 422 393 366 268
EcoO109I rG'GnC_Cy
Cuts at: 2021 2524 2647 2893 3010 2021
Size: 503 123 246 117 4901
Fragments arranged by size:
4901 503 246 123 117
EcoRI G'AATT_C
Cuts at: 2468 3199 3577 2468
Size: 731 378 4781
Fragments arranged by size:
4781 731 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 5890
FspI TGC'GCA
Cuts at: 4396 4396
Size: 5890
HaeII r_GCGC'y
Cuts at: 1678 2399 5037 1678
Size: 721 2638 2531
Fragments arranged by size:
2638 2531 721
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2711 2743 2882 2914 3104
Size: 32 135 32 1139 32 139 32 190
Cuts at: 3104 3136 4531 4563 4605 4637 1373
Size: 32 1395 32 42 32 2626
Fragments arranged by size:
2626 1395 1139 190 139 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2218 3234 3480 669
Size: 988 281 280 1016 246 3079
Fragments arranged by size:
3079 1016 988 281 280 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5890
HpaI GTT'AAC
Cuts at: 3480 3480
Size: 5890
KpnI G_GTAC'C
Cuts at: 3215 3215
Size: 5890
MfeI C'AATT_G
Cuts at: 3489 3489
Size: 5890
MluI A'CGCG_T
Cuts at: 5766 5766
Size: 5890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2058 2172 2220 2580 5004 5188 1305
Size: 753 114 48 360 2424 184 2007
Fragments arranged by size:
2424 2007 753 360 184 114 48
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2966 10
Size: 54 1425 343 1134 2934
Fragments arranged by size:
2934 1425 1134 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 3848 4207 4366 518
Size: 648 924 1758 359 159 2042
Fragments arranged by size:
2042 1758 924 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2185 2283 2352 2491 2631 2653 2765 2878
Size: 579 98 69 139 140 22 112 113
Cuts at: 2878 2938 3306 3983 5131 5376 1606
Size: 60 368 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 579 368 245 140 139
113 112 98 69 60 22
NaeI GCC'GGC
Cuts at: 2598 2598
Size: 5890
NcoI C'CATG_G
Cuts at: 513 1065 2004 2967 513
Size: 552 939 963 3436
Fragments arranged by size:
3436 963 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5890
NgoMIV G'CCGG_C
Cuts at: 2596 2596
Size: 5890
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5890
NotI GC'GGCC_GC
Cuts at: 3337 3337
Size: 5890
NspI r_CATG'y
Cuts at: 1132 1229 3248 4793 1132
Size: 97 2019 1545 2229
Fragments arranged by size:
2229 2019 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5890
PciI A'CATG_T
Cuts at: 4789 4789
Size: 5890
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2881 1108
Size: 1773 4117
PfoI T'CCnGG_A
Cuts at: 2292 2292
Size: 5890
PmeI GTTT'AAAC
Cuts at: 3194 3194
Size: 5890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 5472 5504 3959
Size: 32 1481 32 4345
Fragments arranged by size:
4345 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2021 2524 2647 2893 2021
Size: 503 123 246 5018
Fragments arranged by size:
5018 503 246 123
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5890
PsiI TTA'TAA
Cuts at: 3460 3460
Size: 5890
PspOMI G'GGCC_C
Cuts at: 2117 2117
Size: 5890
PstI C_TGCA'G
Cuts at: 830 3129 3242 830
Size: 2299 113 3478
Fragments arranged by size:
3478 2299 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5890
PvuII CAG'CTG
Cuts at: 2283 2491 2765 2283
Size: 208 274 5408
Fragments arranged by size:
5408 274 208
SacII CC_GC'GG
Cuts at: 2654 2654
Size: 5890
SalI G'TCGA_C
Cuts at: 1936 3232 1936
Size: 1296 4594
SapI GCTCTTCn'nnn_
Cuts at: 2339 2561 2692 2339
Size: 222 131 5537
Fragments arranged by size:
5537 222 131
SbfI CC_TGCA'GG
Cuts at: 3129 3242 3129
Size: 113 5777
ScaI AGT'ACT
Cuts at: 4138 5850 4138
Size: 1712 4178
SfcI C'TryA_G
Cuts at: 826 1045 3125 3238 4373 5054 5245 826
Size: 219 2080 113 1135 681 191 1471
Fragments arranged by size:
2080 1471 1135 681 219 191 113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5890
SmaI CCC'GGG
Cuts at: 1872 2116 3217 1872
Size: 244 1101 4545
Fragments arranged by size:
4545 1101 244
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2477 3994 4895 5157 820
Size: 197 926 534 1517 901 262 1553
Fragments arranged by size:
1553 1517 926 901 534 262 197
SnaBI TAC'GTA
Cuts at: 493 3681 493
Size: 3188 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5890
SphI G_CATG'C
Cuts at: 1132 3248 1132
Size: 2116 3774
SspI AAT'ATT
Cuts at: 5 52 3814 5
Size: 47 3762 2081
Fragments arranged by size:
3762 2081 47
StuI AGG'CCT
Cuts at: 1517 2711 1517
Size: 1194 4696
StyI C'CwwG_G
Cuts at: 513 1065 2004 2601 2967 3329 513
Size: 552 939 597 366 362 3074
Fragments arranged by size:
3074 939 597 552 366 362
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3917 3934 4087 4272 4717 325
Size: 1255 2337 17 153 185 445 1498
Fragments arranged by size:
2337 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2307 4136 5848
Size: 274 80 33 51 1443 330 1829 1712
Cuts at: 5848 96
Size: 138
Fragments arranged by size:
1829 1712 1443 330 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5850 5882 5850
Size: 32 5858
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5890
XbaI T'CTAG_A
Cuts at: 3226 3226
Size: 5890
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5890
XmaI C'CCGG_G
Cuts at: 1870 2114 3215 1870
Size: 244 1101 4545
Fragments arranged by size:
4545 1101 244
XmnI GAAnn'nnTTC
Cuts at: 4019 4019
Size: 5890
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3698 276
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI
SwaI XcmI