Restriction map for
pFN21AB7669
(Circular) (Six-base) MAPSORT of: pFN21AB7669.seq Check: 1382 from: 1 to: 5719
pFN21AB7669 5719 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2933 2933
Size: 5719
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3529 278
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2286 3062 1937
Size: 349 776 4594
Fragments arranged by size:
4594 776 349
Acc65I G'GTAC_C
Cuts at: 3040 3040
Size: 5719
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2743 3782 5166 5434 1632
Size: 1111 1039 1384 268 1917
Fragments arranged by size:
1917 1384 1111 1039 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5522
AflIII A'CryG_T
Cuts at: 1203 4618 5595 1203
Size: 3415 977 1327
Fragments arranged by size:
3415 1327 977
AgeI A'CCGG_T
Cuts at: 4550 4550
Size: 5719
AhdI GACnn_n'nnGTC
Cuts at: 4448 4448
Size: 5719
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2533 1184
Size: 1349 4370
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 5687
AlwNI CAG_nnn'CTG
Cuts at: 2263 3462 5034 2263
Size: 1199 1572 2948
Fragments arranged by size:
2948 1572 1199
ApaI G_GGCC'C
Cuts at: 2694 2705 2694
Size: 11 5708
ApaLI G'TGCA_C
Cuts at: 3776 4932 3776
Size: 1156 4563
ApoI r'AATT_y
Cuts at: 1493 3028 3254 3406 5515 1493
Size: 1535 226 152 2109 1697
Fragments arranged by size:
2109 1697 1535 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4273 160
Size: 872 3241 1606
Fragments arranged by size:
3241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 2144 2418 2439 2693 3044 1870
Size: 73 201 274 21 254 351 4545
Fragments arranged by size:
4545 351 274 254 201 73 21
AvrII C'CTAG_G
Cuts at: 2429 2429
Size: 5719
BamHI G'GATC_C
Cuts at: 3049 3416 3049
Size: 367 5352
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2951 3040 4495 618
Size: 325 217 627 1164 89 1455 1842
Fragments arranged by size:
1842 1455 1164 627 325 217 89
BanII G_rGCy'C
Cuts at: 1391 2425 2616 2694 2705 2735 3038 1391
Size: 1034 191 78 11 30 303 4072
Fragments arranged by size:
4072 1034 303 191 78 30 11
BbeI G_GCGC'C
Cuts at: 2955 2955
Size: 5719
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2076 928
Size: 971 177 4571
Fragments arranged by size:
4571 971 177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3910 3944 1903
Size: 34 1973 34 3678
Fragments arranged by size:
3678 1973 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3611 4821 3611
Size: 1210 4509
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4330 136
Size: 107 122 71 631 605 2658 1525
Fragments arranged by size:
2658 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5714 5714
Size: 5719
BlpI GC'TnA_GC
Cuts at: 2734 3136 2734
Size: 402 5317
Bme1580I G_kGCm'C
Cuts at: 1790 2172 2694 2705 3780 4936 1790
Size: 382 522 11 1075 1156 2573
Fragments arranged by size:
2573 1156 1075 522 382 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5719
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2239 3450 4408
Size: 416 53 83 399 24 822 1211 958
Cuts at: 4408 442
Size: 1753
Fragments arranged by size:
1753 1211 958 822 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2537 2615 2657 4379 1239
Size: 950 348 78 42 1722 2579
Fragments arranged by size:
2579 1722 950 348 78 42
Bpu10I CC'TnA_GC
Cuts at: 2745 2745
Size: 5719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3844 4709 5007 3844
Size: 865 298 4556
Fragments arranged by size:
4556 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2147 2428 2941 4382 882
Size: 617 648 281 513 1441 2219
Fragments arranged by size:
2219 1441 648 617 513 281
BsaAI yAC'GTr
Cuts at: 493 1204 3510 493
Size: 711 2306 2702
Fragments arranged by size:
2702 2306 711
BsaBI GATnn'nnATC
Cuts at: 5713 5713
Size: 5719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2952 3526 3908 4543
Size: 53 83 186 936 1419 574 382 635
Cuts at: 4543 275
Size: 1451
Fragments arranged by size:
1451 1419 936 635 574 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4152 4550 4824 4971 5382 4152
Size: 398 274 147 411 4489
Fragments arranged by size:
4489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2018 2024 2048 2054 2987
Size: 30 165 30 615 6 24 6 933
Cuts at: 2987 3017 1178
Size: 30 3880
Fragments arranged by size:
3880 933 615 165 30 30 30 24
6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2044 2459 2483 2999 1186
Size: 858 415 24 516 3906
Fragments arranged by size:
3906 858 516 415 24
BseYI C'CCAG_C
Cuts at: 2271 2413 4922 5418 2271
Size: 142 2509 496 2572
Fragments arranged by size:
2572 2509 496 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2068 1163
Size: 573 332 4814
Fragments arranged by size:
4814 573 332
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2283 2548 3169 3930 4958 1540
Size: 275 186 282 265 621 761 1028 2301
Fragments arranged by size:
2301 1028 761 621 282 275 265 186
BsiHKAI G_wGCw'C
Cuts at: 2616 3780 3865 4936 2616
Size: 1164 85 1071 3399
Fragments arranged by size:
3399 1164 1071 85
BsmI GAATG_Cn'
Cuts at: 3230 3323 3230
Size: 93 5626
BsmBI CGTCTCn'nnnn_
Cuts at: 2441 2441
Size: 5719
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2425 2616 2694 2705 2735 3038
Size: 399 382 253 191 78 11 30 303
Cuts at: 3038 3780 3865 4936 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 382 303 253 191
85 78 30 11
BspEI T'CCGG_A
Cuts at: 5382 5382
Size: 5719
BspHI T'CATG_A
Cuts at: 3608 5338 3608
Size: 1730 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2933 3074 844
Size: 2089 141 3489
Fragments arranged by size:
3489 2089 141
BsrBI CCG'CTC
Cuts at: 1394 1823 3606 1394
Size: 429 1783 3507
Fragments arranged by size:
3507 1783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4214 4388 65
Size: 1183 2966 174 1396
Fragments arranged by size:
2966 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2138 2526 2740 2948 4363 4550 1700
Size: 110 328 388 214 208 1415 187 2869
Fragments arranged by size:
2869 1415 388 328 214 208 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5719
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5719
BssSI C'ACGA_G
Cuts at: 3779 4791 3779
Size: 1012 4707
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5719
BstBI TT'CG_AA
Cuts at: 4523 4523
Size: 5719
BstEII G'GTnAC_C
Cuts at: 1184 2176 1184
Size: 992 4727
BstYI r'GATC_y
Cuts at: 1972 2673 3049 3416 3801 3818 5270 5356 5368
Size: 701 376 367 385 17 1452 86 12
Cuts at: 5368 5714 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 701 385 376 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2172 2238 513
Size: 552 1107 66 3994
Fragments arranged by size:
3994 1107 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4980
Fragments arranged by size:
4980 683 56
BtsI GCAGTG_nn'
Cuts at: 2350 2368 2977 3235 4029 4049 2350
Size: 18 609 258 794 20 4020
Fragments arranged by size:
4020 794 609 258 20 18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 3023 3023
Size: 5719
DraIII CAC_nnn'GTG
Cuts at: 2855 2855
Size: 5719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4726 809
Size: 507 3410 1802
Fragments arranged by size:
3410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3166 4055 5684
Size: 54 1425 50 275 18 1336 889 1629
Cuts at: 5684 8
Size: 43
Fragments arranged by size:
1629 1425 1336 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3166 1537
Size: 275 1354 4090
Fragments arranged by size:
4090 1354 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2239 2460 2679 3649 1353
Size: 886 221 219 970 3423
Fragments arranged by size:
3423 970 886 221 219
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2364 2545 2573 2726 4306 4680
Size: 1064 138 775 181 28 153 1580 374
Cuts at: 4680 4826 387
Size: 146 1280
Fragments arranged by size:
1580 1280 1064 775 374 181 153 146
138 28
EcoICRI GAG'CTC
Cuts at: 2614 2614
Size: 5719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2537 2615 2657 2743 3782 4379
Size: 393 557 348 78 42 86 1039 597
Cuts at: 4379 5166 5434 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1039 787 597 557 393 348 268
86 78 42
EcoO109I rG'GnC_Cy
Cuts at: 2200 2690 2200
Size: 490 5229
EcoRI G'AATT_C
Cuts at: 3028 3406 3028
Size: 378 5341
FspI TGC'GCA
Cuts at: 4225 4225
Size: 5719
HaeII r_GCGC'y
Cuts at: 1678 2955 4866 1678
Size: 1277 1911 2531
Fragments arranged by size:
2531 1911 1277
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2024 2050 2056 2582
Size: 32 135 32 446 6 26 6 526
Cuts at: 2582 2614 2824 2856 4360 4392 4434 4466 1373
Size: 32 210 32 1504 32 42 32 2626
Fragments arranged by size:
2626 1504 526 446 210 135 42 32
32 32 32 32 32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2287 2893 3063 3309 669
Size: 988 281 349 606 170 246 3079
Fragments arranged by size:
3079 988 606 349 281 246 170
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5719
HpaI GTT'AAC
Cuts at: 2893 3309 2893
Size: 416 5303
KasI G'GCGC_C
Cuts at: 2951 2951
Size: 5719
KpnI G_GTAC'C
Cuts at: 3044 3044
Size: 5719
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2553 2580 4833 5017 1305
Size: 1248 27 2253 184 2007
Fragments arranged by size:
2253 2007 1248 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3897
Fragments arranged by size:
3897 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3677 4036 4195 518
Size: 648 2511 359 159 2042
Fragments arranged by size:
2511 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2382 2707 2763 2967 3135 3812 4960 5205
Size: 776 325 56 204 168 677 1148 245
Cuts at: 5205 1606
Size: 2120
Fragments arranged by size:
2120 1148 776 677 325 245 204 168
56
NaeI GCC'GGC
Cuts at: 2950 2950
Size: 5719
NarI GG'CG_CC
Cuts at: 2952 2952
Size: 5719
NcoI C'CATG_G
Cuts at: 513 1065 2238 513
Size: 552 1173 3994
Fragments arranged by size:
3994 1173 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NgoMIV G'CCGG_C
Cuts at: 2948 2948
Size: 5719
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5719
NotI GC'GGCC_GC
Cuts at: 3166 3166
Size: 5719
NspI r_CATG'y
Cuts at: 1132 1229 2302 3077 4622 1132
Size: 97 1073 775 1545 2229
Fragments arranged by size:
2229 1545 1073 775 97
PasI CC'CwG_GG
Cuts at: 1348 2251 1348
Size: 903 4816
PciI A'CATG_T
Cuts at: 4618 4618
Size: 5719
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5719
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3019 3788 3820 5301 5333 2987
Size: 32 769 32 1481 32 3373
Fragments arranged by size:
3373 1481 769 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2200 2200
Size: 5719
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5719
PsiI TTA'TAA
Cuts at: 3289 3289
Size: 5719
PspOMI G'GGCC_C
Cuts at: 2690 2701 2690
Size: 11 5708
PstI C_TGCA'G
Cuts at: 830 3071 830
Size: 2241 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
PvuII CAG'CTG
Cuts at: 2763 2763
Size: 5719
SacI G_AGCT'C
Cuts at: 2616 2616
Size: 5719
SalI G'TCGA_C
Cuts at: 1936 2285 3061 1936
Size: 349 776 4594
Fragments arranged by size:
4594 776 349
SbfI CC_TGCA'GG
Cuts at: 3071 3071
Size: 5719
ScaI AGT'ACT
Cuts at: 3967 5679 3967
Size: 1712 4007
SfcI C'TryA_G
Cuts at: 826 1045 2338 3067 4202 4883 5074 826
Size: 219 1293 729 1135 681 191 1471
Fragments arranged by size:
1471 1293 1135 729 681 219 191
SfoI GGC'GCC
Cuts at: 2953 2953
Size: 5719
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 2948 1700
Size: 1248 4471
SmaI CCC'GGG
Cuts at: 1872 2695 3046 1872
Size: 823 351 4545
Fragments arranged by size:
4545 823 351
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3823 4724 4986 820
Size: 197 926 1880 901 262 1553
Fragments arranged by size:
1880 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3510 493
Size: 3017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 3077 1132
Size: 1945 3774
SrfI GCCC'GGGC
Cuts at: 2695 2695
Size: 5719
SspI AAT'ATT
Cuts at: 5 52 3643 5
Size: 47 3591 2081
Fragments arranged by size:
3591 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5719
StyI C'CwwG_G
Cuts at: 513 1065 2238 2429 3158 513
Size: 552 1173 191 729 3074
Fragments arranged by size:
3074 1173 729 552 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2125 2558 2562 3746 3763 3916 4101
Size: 1255 545 433 4 1184 17 153 185
Cuts at: 4101 4546 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1184 545 445 433 185 153
17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3965 5677 96
Size: 274 80 33 51 1443 1988 1712 138
Fragments arranged by size:
1988 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2192 2224 5679 5711 2192
Size: 32 3455 32 2200
Fragments arranged by size:
3455 2200 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5719
XbaI T'CTAG_A
Cuts at: 3055 3055
Size: 5719
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5719
XmaI C'CCGG_G
Cuts at: 1870 2693 3044 1870
Size: 823 351 4545
Fragments arranged by size:
4545 823 351
XmnI GAAnn'nnTTC
Cuts at: 3848 3848
Size: 5719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3527 276
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbvCI BfrBI BplI BsiWI BstXI
BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SwaI XcmI