Restriction map for
pFN21AB7668
(Circular) (Six-base) MAPSORT of: pFN21AB7668.seq Check: 2598 from: 1 to: 5911
pFN21AB7668 5911 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2285 2285
Size: 5911
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3721 278
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3254 1937
Size: 1317 4594
Acc65I G'GTAC_C
Cuts at: 3232 3232
Size: 5911
AclI AA'CG_TT
Cuts at: 1563 4038 4411 1563
Size: 2475 373 3063
Fragments arranged by size:
3063 2475 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3974 5358 5626 1632
Size: 2342 1384 268 1917
Fragments arranged by size:
2342 1917 1384 268
AfeI AGC'GCT
Cuts at: 2979 2979
Size: 5911
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5714
AflIII A'CryG_T
Cuts at: 1203 4810 5787 1203
Size: 3607 977 1327
Fragments arranged by size:
3607 1327 977
AgeI A'CCGG_T
Cuts at: 4742 4742
Size: 5911
AhdI GACnn_n'nnGTC
Cuts at: 4640 4640
Size: 5911
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5911
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5911
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2684 2716 2684
Size: 32 5879
AlwNI CAG_nnn'CTG
Cuts at: 3654 5226 3654
Size: 1572 4339
ApaI G_GGCC'C
Cuts at: 3123 3123
Size: 5911
ApaLI G'TGCA_C
Cuts at: 2012 3968 5124 2012
Size: 1956 1156 2799
Fragments arranged by size:
2799 1956 1156
ApoI r'AATT_y
Cuts at: 1493 3220 3446 3598 5707 1493
Size: 1727 226 152 2109 1697
Fragments arranged by size:
2109 1727 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4465 160
Size: 872 3433 1606
Fragments arranged by size:
3433 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
AvaI C'yCGr_G
Cuts at: 1870 1943 2530 3062 3185 3236 1870
Size: 73 587 532 123 51 4545
Fragments arranged by size:
4545 587 532 123 73 51
AvrII C'CTAG_G
Cuts at: 2734 2734
Size: 5911
BamHI G'GATC_C
Cuts at: 3241 3608 3241
Size: 367 5544
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2534 2738 3146 3232 4687
Size: 325 217 627 747 204 408 86 1455
Cuts at: 4687 618
Size: 1842
Fragments arranged by size:
1842 1455 747 627 408 325 217 204
86
BanII G_rGCy'C
Cuts at: 1391 2319 2369 3062 3123 3192 3230 1391
Size: 928 50 693 61 69 38 4072
Fragments arranged by size:
4072 928 693 69 61 50 38
BbeI G_GCGC'C
Cuts at: 2742 2742
Size: 5911
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4940
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4102 4136 1903
Size: 34 2165 34 3678
Fragments arranged by size:
3678 2165 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3803 5013 3803
Size: 1210 4701
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5911
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3078 4522 136
Size: 107 122 71 631 605 1406 1444 1525
Fragments arranged by size:
1525 1444 1406 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5906 5906
Size: 5911
BlpI GC'TnA_GC
Cuts at: 2726 3328 2726
Size: 602 5309
Bme1580I G_kGCm'C
Cuts at: 1790 2016 2537 3083 3123 3972 5128 1790
Size: 226 521 546 40 849 1156 2573
Fragments arranged by size:
2573 1156 849 546 521 226 40
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5911
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3642 4600 442
Size: 416 53 83 399 24 2225 958 1753
Fragments arranged by size:
2225 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5911
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 5911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2423 2600 2917 4571 1239
Size: 1184 177 317 1654 2579
Fragments arranged by size:
2579 1654 1184 317 177
Bpu10I CC'TnA_GC
Cuts at: 2363 2363
Size: 5911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2363 2573 4036 4901 5199 2363
Size: 210 1463 865 298 3075
Fragments arranged by size:
3075 1463 865 298 210
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2645 4574 882
Size: 617 1146 1929 2219
Fragments arranged by size:
2219 1929 1146 617
BsaAI yAC'GTr
Cuts at: 493 1204 3702 493
Size: 711 2498 2702
Fragments arranged by size:
2702 2498 711
BsaBI GATnn'nnATC
Cuts at: 5905 5905
Size: 5911
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2739 2966 3718 4100
Size: 53 83 186 936 1206 227 752 382
Cuts at: 4100 4735 275
Size: 635 1451
Fragments arranged by size:
1451 1206 936 752 635 382 227 186
83 53
BsaWI w'CCGG_w
Cuts at: 2760 4344 4742 5016 5163 5574 2760
Size: 1584 398 274 147 411 3097
Fragments arranged by size:
3097 1584 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5686
Fragments arranged by size:
5686 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2327 2534 1186
Size: 1141 207 4563
Fragments arranged by size:
4563 1141 207
BseYI C'CCAG_C
Cuts at: 2222 2785 2971 3122 5114 5610 2222
Size: 563 186 151 1992 496 2523
Fragments arranged by size:
2523 1992 563 496 186 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3071 1163
Size: 573 1335 4003
Fragments arranged by size:
4003 1335 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2047 2794 2928 3361 4122 5150
Size: 275 186 46 747 134 433 761 1028
Cuts at: 5150 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 747 433 275 186 134
46
BsiHKAI G_wGCw'C
Cuts at: 2016 2319 2369 3192 3972 4057 5128 2016
Size: 303 50 823 780 85 1071 2799
Fragments arranged by size:
2799 1071 823 780 303 85 50
BsmI GAATG_Cn'
Cuts at: 3422 3515 3422
Size: 93 5818
BsmBI CGTCTCn'nnnn_
Cuts at: 2462 2492 2576 2462
Size: 30 84 5797
Fragments arranged by size:
5797 84 30
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2319 2369 2537 3062 3083 3123
Size: 399 226 303 50 168 525 21 40
Cuts at: 3123 3192 3230 3972 4057 5128 1391
Size: 69 38 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 525 399 303 226 168
85 69 50 40 38 21
BspEI T'CCGG_A
Cuts at: 2760 5574 2760
Size: 2814 3097
BspHI T'CATG_A
Cuts at: 3800 5530 3800
Size: 1730 4181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2285 3266 844
Size: 1441 981 3489
Fragments arranged by size:
3489 1441 981
BsrBI CCG'CTC
Cuts at: 1394 1823 2032 2992 3144 3798 1394
Size: 429 209 960 152 654 3507
Fragments arranged by size:
3507 960 654 429 209 152
BsrDI GCAATG_nn'
Cuts at: 65 1248 4406 4580 65
Size: 1183 3158 174 1396
Fragments arranged by size:
3158 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2021 2198 2306 2654 2996 4555 4742
Size: 110 211 177 108 348 342 1559 187
Cuts at: 4742 1700
Size: 2869
Fragments arranged by size:
2869 1559 348 342 211 187 177 110
108
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5911
BssHII G'CGCG_C
Cuts at: 1925 2205 1925
Size: 280 5631
BssSI C'ACGA_G
Cuts at: 3971 4983 3971
Size: 1012 4899
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2193 3094 794
Size: 1399 901 3611
Fragments arranged by size:
3611 1399 901
BstBI TT'CG_AA
Cuts at: 4715 4715
Size: 5911
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5911
BstXI CCAn_nnnn'nTGG
Cuts at: 2230 2578 2941 2230
Size: 348 363 5200
Fragments arranged by size:
5200 363 348
BstYI r'GATC_y
Cuts at: 1972 3241 3608 3993 4010 5462 5548 5560 5906
Size: 1269 367 385 17 1452 86 12 346
Cuts at: 5906 1972
Size: 1977
Fragments arranged by size:
1977 1452 1269 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2768 3009 513
Size: 552 1703 241 3415
Fragments arranged by size:
3415 1703 552 241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5172
Fragments arranged by size:
5172 683 56
BtsI GCAGTG_nn'
Cuts at: 2014 2447 3427 4221 4241 2014
Size: 433 980 794 20 3684
Fragments arranged by size:
3684 980 794 433 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5876
DraI TTT'AAA
Cuts at: 3215 3215
Size: 5911
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4918 809
Size: 507 3602 1802
Fragments arranged by size:
3602 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2502 2791 2925
Size: 54 1425 50 275 18 672 289 134
Cuts at: 2925 3358 4247 5876 8
Size: 433 889 1629 43
Fragments arranged by size:
1629 1425 889 672 433 289 275 134
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2791 2925 3358 1537
Size: 275 979 134 433 4090
Fragments arranged by size:
4090 979 433 275 134
EarI CTCTTCn'nnn_
Cuts at: 1353 3841 1353
Size: 2488 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4498 4872 5018 387
Size: 1064 138 2909 374 146 1280
Fragments arranged by size:
2909 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2317 2367 3190 2317
Size: 50 823 5038
Fragments arranged by size:
5038 823 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 2600 2917 3974 4571 5358 5626
Size: 393 791 177 317 1057 597 787 268
Cuts at: 5626 1239
Size: 1524
Fragments arranged by size:
1524 1057 791 787 597 393 317 268
177
EcoNI CCTnn'n_nnAGG
Cuts at: 2381 2381
Size: 5911
EcoRI G'AATT_C
Cuts at: 3220 3598 3220
Size: 378 5533
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 5911
FspI TGC'GCA
Cuts at: 2237 4417 2237
Size: 2180 3731
HaeII r_GCGC'y
Cuts at: 1678 2182 2733 2742 2981 5058 1678
Size: 504 551 9 239 2077 2531
Fragments arranged by size:
2531 2077 551 504 239 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3023 3055 4552 4584 4626
Size: 32 135 32 1451 32 1497 32 42
Cuts at: 4626 4658 1373
Size: 32 2626
Fragments arranged by size:
2626 1497 1451 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3255 3501 669
Size: 988 281 1317 246 3079
Fragments arranged by size:
3079 1317 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5911
HpaI GTT'AAC
Cuts at: 3501 3501
Size: 5911
KasI G'GCGC_C
Cuts at: 2738 2738
Size: 5911
KpnI G_GTAC'C
Cuts at: 3236 3236
Size: 5911
MfeI C'AATT_G
Cuts at: 3510 3510
Size: 5911
MluI A'CGCG_T
Cuts at: 5787 5787
Size: 5911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2469 5025 5209 1305
Size: 1164 2556 184 2007
Fragments arranged by size:
2556 2007 1164 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4089
Fragments arranged by size:
4089 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2279 2678 3032 3869 4228 4387
Size: 648 843 270 399 354 837 359 159
Cuts at: 4387 518
Size: 2042
Fragments arranged by size:
2042 843 837 648 399 359 354 270
159
MspA1I CmG'CkG
Cuts at: 1606 2299 2578 2641 2725 2770 2785 2971 3011
Size: 693 279 63 84 45 15 186 40
Cuts at: 3011 3114 3153 3162 3327 4004 5152 5397 1606
Size: 103 39 9 165 677 1148 245 2120
Fragments arranged by size:
2120 1148 693 677 279 245 186 165
103 84 63 45 40 39 15 9
NaeI GCC'GGC
Cuts at: 2200 2998 2200
Size: 798 5113
NarI GG'CG_CC
Cuts at: 2739 2739
Size: 5911
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5359
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5911
NgoMIV G'CCGG_C
Cuts at: 2198 2996 2198
Size: 798 5113
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5911
NotI GC'GGCC_GC
Cuts at: 2791 3358 2791
Size: 567 5344
NspI r_CATG'y
Cuts at: 1132 1229 2668 2779 3269 4814 1132
Size: 97 1439 111 490 1545 2229
Fragments arranged by size:
2229 1545 1439 490 111 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5911
PciI A'CATG_T
Cuts at: 4810 4810
Size: 5911
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5911
PfoI T'CCnGG_A
Cuts at: 2439 2439
Size: 5911
PmeI GTTT'AAAC
Cuts at: 3215 3215
Size: 5911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2324 2356 2976 3008 3980 4012 5493 5525 2324
Size: 32 620 32 972 32 1481 32 2710
Fragments arranged by size:
2710 1481 972 620 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3169 1613
Size: 1556 4355
PsiI TTA'TAA
Cuts at: 3481 3481
Size: 5911
PspOMI G'GGCC_C
Cuts at: 3119 3119
Size: 5911
PstI C_TGCA'G
Cuts at: 830 2454 3263 830
Size: 1624 809 3478
Fragments arranged by size:
3478 1624 809
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5911
PvuII CAG'CTG
Cuts at: 2299 2578 2641 2725 2299
Size: 279 63 84 5485
Fragments arranged by size:
5485 279 84 63
RsrII CG'GwC_CG
Cuts at: 2416 2416
Size: 5911
SacI G_AGCT'C
Cuts at: 2319 2369 3192 2319
Size: 50 823 5038
Fragments arranged by size:
5038 823 50
SacII CC_GC'GG
Cuts at: 2771 3012 2771
Size: 241 5670
SalI G'TCGA_C
Cuts at: 1936 3253 1936
Size: 1317 4594
SbfI CC_TGCA'GG
Cuts at: 3263 3263
Size: 5911
ScaI AGT'ACT
Cuts at: 4159 5871 4159
Size: 1712 4199
SexAI A'CCwGG_T
Cuts at: 2229 2229
Size: 5911
SfcI C'TryA_G
Cuts at: 826 1045 2450 3157 3259 4394 5075 5266 826
Size: 219 1405 707 102 1135 681 191 1471
Fragments arranged by size:
1471 1405 1135 707 681 219 191 102
SfoI GGC'GCC
Cuts at: 2740 2740
Size: 5911
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
SgrAI Cr'CCGG_yG
Cuts at: 1700 2306 1700
Size: 606 5305
SmaI CCC'GGG
Cuts at: 1872 3238 1872
Size: 1366 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2378 2588 4015 4916 5178 820
Size: 197 926 435 210 1427 901 262 1553
Fragments arranged by size:
1553 1427 926 901 435 262 210 197
SnaBI TAC'GTA
Cuts at: 493 3702 493
Size: 3209 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5911
SphI G_CATG'C
Cuts at: 1132 2779 3269 1132
Size: 1647 490 3774
Fragments arranged by size:
3774 1647 490
SspI AAT'ATT
Cuts at: 5 52 3835 5
Size: 47 3783 2081
Fragments arranged by size:
3783 2081 47
StuI AGG'CCT
Cuts at: 1517 3072 1517
Size: 1555 4356
StyI C'CwwG_G
Cuts at: 513 1065 2734 2851 3350 513
Size: 552 1669 117 499 3074
Fragments arranged by size:
3074 1669 552 499 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3938 3955 4108 4293 4738 325
Size: 1255 2358 17 153 185 445 1498
Fragments arranged by size:
2358 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4157 5869 96
Size: 274 80 33 51 1443 2180 1712 138
Fragments arranged by size:
2180 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2060 2092 5871 5903 2060
Size: 32 3779 32 2068
Fragments arranged by size:
3779 2068 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2485 1316
Size: 1169 4742
XbaI T'CTAG_A
Cuts at: 3247 3247
Size: 5911
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5911
XmaI C'CCGG_G
Cuts at: 1870 3236 1870
Size: 1366 4545
XmnI GAAnn'nnTTC
Cuts at: 2132 2339 4040 2132
Size: 207 1701 4003
Fragments arranged by size:
4003 1701 207
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3719 276
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII
EcoO109I EcoRV FseI FspAI NruI NsiI PacI PmlI
PpuMI PspXI PsrI SanDI SapI SfiI SrfI SwaI
XcmI