Restriction map for
pFN21AB7664
(Circular) (Six-base) MAPSORT of: pFN21AB7664.seq Check: 4606 from: 1 to: 5893
pFN21AB7664 5893 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3703 278
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2857 3236 1937
Size: 920 379 4594
Fragments arranged by size:
4594 920 379
Acc65I G'GTAC_C
Cuts at: 3214 3214
Size: 5893
AclI AA'CG_TT
Cuts at: 1563 4020 4393 1563
Size: 2457 373 3063
Fragments arranged by size:
3063 2457 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3956 5340 5608 1632
Size: 2324 1384 268 1917
Fragments arranged by size:
2324 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5696
AflIII A'CryG_T
Cuts at: 1203 4792 5769 1203
Size: 3589 977 1327
Fragments arranged by size:
3589 1327 977
AgeI A'CCGG_T
Cuts at: 4724 4724
Size: 5893
AhdI GACnn_n'nnGTC
Cuts at: 4622 4622
Size: 5893
AleI CACnn'nnGTG
Cuts at: 1166 2151 1166
Size: 985 4908
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5893
AlwNI CAG_nnn'CTG
Cuts at: 2518 3636 5208 2518
Size: 1118 1572 3203
Fragments arranged by size:
3203 1572 1118
ApaLI G'TGCA_C
Cuts at: 3950 5106 3950
Size: 1156 4737
ApoI r'AATT_y
Cuts at: 1493 3080 3202 3428 3580 5689 1493
Size: 1587 122 226 152 2109 1697
Fragments arranged by size:
2109 1697 1587 226 152 122
AseI AT'TA_AT
Cuts at: 160 1032 4447 160
Size: 872 3415 1606
Fragments arranged by size:
3415 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
AvaI C'yCGr_G
Cuts at: 1870 1943 2191 2214 3218 1870
Size: 73 248 23 1004 4545
Fragments arranged by size:
4545 1004 248 73 23
BamHI G'GATC_C
Cuts at: 3223 3590 3223
Size: 367 5526
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2656 3214 4669 618
Size: 325 217 627 869 558 1455 1842
Fragments arranged by size:
1842 1455 869 627 558 325 217
BanII G_rGCy'C
Cuts at: 1391 2215 2751 3212 1391
Size: 824 536 461 4072
Fragments arranged by size:
4072 824 536 461
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2053 928
Size: 971 154 4768
Fragments arranged by size:
4768 971 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4084 4118 1903
Size: 34 2147 34 3678
Fragments arranged by size:
3678 2147 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2774 3785 4995 2774
Size: 1011 1210 3672
Fragments arranged by size:
3672 1210 1011
BclI T'GATC_A
Cuts at: 1548 2887 1548
Size: 1339 4554
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4504 136
Size: 107 122 71 631 605 337 2495 1525
Fragments arranged by size:
2495 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5888 5888
Size: 5893
BlpI GC'TnA_GC
Cuts at: 3310 3310
Size: 5893
Bme1580I G_kGCm'C
Cuts at: 1790 2661 2698 3954 5110 1790
Size: 871 37 1256 1156 2573
Fragments arranged by size:
2573 1256 1156 871 37
BmgBI CAC'GTC
Cuts at: 1318 2065 1318
Size: 747 5146
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2861 3624 4582
Size: 416 53 83 399 24 1444 763 958
Cuts at: 4582 442
Size: 1753
Fragments arranged by size:
1753 1444 958 763 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4553 1239
Size: 3314 2579
Bpu10I CC'TnA_GC
Cuts at: 2526 3001 2526
Size: 475 5418
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2723 4018 4883 5181 2723
Size: 1295 865 298 3435
Fragments arranged by size:
3435 1295 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4556 882
Size: 617 3057 2219
Fragments arranged by size:
3057 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2762 3684 493
Size: 711 1558 922 2702
Fragments arranged by size:
2702 1558 922 711
BsaBI GATnn'nnATC
Cuts at: 5887 5887
Size: 5893
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2782 3700 4082 4717
Size: 53 83 186 936 1249 918 382 635
Cuts at: 4717 275
Size: 1451
Fragments arranged by size:
1451 1249 936 918 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2140 2150 4326 4724 4998 5145 5556 2140
Size: 10 2176 398 274 147 411 2477
Fragments arranged by size:
2477 2176 411 398 274 147 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5668
Fragments arranged by size:
5668 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2232 2268 3179 3182 3185 1186
Size: 1046 36 911 3 3 3894
Fragments arranged by size:
3894 1046 911 36 3 3
BseYI C'CCAG_C
Cuts at: 5096 5592 5096
Size: 496 5397
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5320
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3343 4104 5132 1540
Size: 275 186 1342 761 1028 2301
Fragments arranged by size:
2301 1342 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3954 4039 5110 3954
Size: 85 1071 4737
Fragments arranged by size:
4737 1071 85
BsmI GAATG_Cn'
Cuts at: 3404 3497 3404
Size: 93 5800
BsmBI CGTCTCn'nnnn_
Cuts at: 2108 2169 2789 2108
Size: 61 620 5212
Fragments arranged by size:
5212 620 61
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2215 2661 2698 2751 3212 3954 4039
Size: 399 425 446 37 53 461 742 85
Cuts at: 4039 5110 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 461 446 425 399 85
53 37
BspEI T'CCGG_A
Cuts at: 2140 5556 2140
Size: 3416 2477
BspHI T'CATG_A
Cuts at: 3782 5512 3782
Size: 1730 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2526 3248 844
Size: 1682 722 3489
Fragments arranged by size:
3489 1682 722
BsrBI CCG'CTC
Cuts at: 1394 1823 2190 2539 3780 1394
Size: 429 367 349 1241 3507
Fragments arranged by size:
3507 1241 429 367 349
BsrDI GCAATG_nn'
Cuts at: 65 1248 2868 3013 4388 4562 65
Size: 1183 1620 145 1375 174 1396
Fragments arranged by size:
1620 1396 1375 1183 174 145
BsrFI r'CCGG_y
Cuts at: 1700 1810 2040 2074 2182 2226 4537 4724 1700
Size: 110 230 34 108 44 2311 187 2869
Fragments arranged by size:
2869 2311 230 187 110 108 44 34
BsrGI T'GTAC_A
Cuts at: 96 2823 96
Size: 2727 3166
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5893
BssSI C'ACGA_G
Cuts at: 3953 4965 3953
Size: 1012 4881
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5893
BstBI TT'CG_AA
Cuts at: 4697 4697
Size: 5893
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5893
BstXI CCAn_nnnn'nTGG
Cuts at: 2153 2153
Size: 5893
BstYI r'GATC_y
Cuts at: 1972 3223 3590 3975 3992 5444 5530 5542 5888
Size: 1251 367 385 17 1452 86 12 346
Cuts at: 5888 1972
Size: 1977
Fragments arranged by size:
1977 1452 1251 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2257 2257
Size: 5893
BtgI C'CryG_G
Cuts at: 513 1065 2200 513
Size: 552 1135 4206
Fragments arranged by size:
4206 1135 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2555 487
Size: 683 56 1329 3825
Fragments arranged by size:
3825 1329 683 56
BtsI GCAGTG_nn'
Cuts at: 2608 3409 4203 4223 2608
Size: 801 794 20 4278
Fragments arranged by size:
4278 801 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5893
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5858
DraI TTT'AAA
Cuts at: 2797 3197 2797
Size: 400 5493
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4900 809
Size: 507 3584 1802
Fragments arranged by size:
3584 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2965 3340 4229
Size: 54 1425 50 275 18 1135 375 889
Cuts at: 4229 5858 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1135 889 375 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3340 1537
Size: 275 1528 4090
Fragments arranged by size:
4090 1528 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2012 2529 2965 3823 1353
Size: 659 517 436 858 3423
Fragments arranged by size:
3423 858 659 517 436
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2157 4480 4854 5000 387
Size: 1064 138 568 2323 374 146 1280
Fragments arranged by size:
2323 1280 1064 568 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3956 4553 5340 5608 1239
Size: 393 2324 597 787 268 1524
Fragments arranged by size:
2324 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3202 3580 3202
Size: 378 5515
FspI TGC'GCA
Cuts at: 4399 4399
Size: 5893
HaeII r_GCGC'y
Cuts at: 1678 5040 1678
Size: 3362 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2879 2911 2984 3016 3090
Size: 32 135 32 1307 32 73 32 74
Cuts at: 3090 3122 4534 4566 4608 4640 1373
Size: 32 1412 32 42 32 2626
Fragments arranged by size:
2626 1412 1307 135 74 73 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2858 3237 3483 669
Size: 988 281 920 379 246 3079
Fragments arranged by size:
3079 988 920 379 281 246
HindIII A'AGCT_T
Cuts at: 748 2677 748
Size: 1929 3964
HpaI GTT'AAC
Cuts at: 3483 3483
Size: 5893
KpnI G_GTAC'C
Cuts at: 3218 3218
Size: 5893
MfeI C'AATT_G
Cuts at: 2883 3492 2883
Size: 609 5284
MluI A'CGCG_T
Cuts at: 5769 5769
Size: 5893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3105 5007 5191 1305
Size: 1800 1902 184 2007
Fragments arranged by size:
2007 1902 1800 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4071
Fragments arranged by size:
4071 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2151 3851 4210 4369 518
Size: 648 985 1700 359 159 2042
Fragments arranged by size:
2042 1700 985 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2202 3309 3986 5134 5379 1606
Size: 596 1107 677 1148 245 2120
Fragments arranged by size:
2120 1148 1107 677 596 245
NaeI GCC'GGC
Cuts at: 2042 2076 2184 2042
Size: 34 108 5751
Fragments arranged by size:
5751 108 34
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5341
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5893
NgoMIV G'CCGG_C
Cuts at: 2040 2074 2182 2040
Size: 34 108 5751
Fragments arranged by size:
5751 108 34
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5893
NotI GC'GGCC_GC
Cuts at: 3340 3340
Size: 5893
NspI r_CATG'y
Cuts at: 1132 1229 2915 3251 4796 1132
Size: 97 1686 336 1545 2229
Fragments arranged by size:
2229 1686 1545 336 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5893
PciI A'CATG_T
Cuts at: 4792 4792
Size: 5893
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5893
PmeI GTTT'AAAC
Cuts at: 3197 3197
Size: 5893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 5475 5507 3962
Size: 32 1481 32 4348
Fragments arranged by size:
4348 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5893
PsiI TTA'TAA
Cuts at: 2550 3463 2550
Size: 913 4980
PstI C_TGCA'G
Cuts at: 830 2523 3245 830
Size: 1693 722 3478
Fragments arranged by size:
3478 1693 722
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5893
SacII CC_GC'GG
Cuts at: 2203 2203
Size: 5893
SalI G'TCGA_C
Cuts at: 1936 2856 3235 1936
Size: 920 379 4594
Fragments arranged by size:
4594 920 379
SapI GCTCTTCn'nnn_
Cuts at: 2012 2529 2012
Size: 517 5376
SbfI CC_TGCA'GG
Cuts at: 2523 3245 2523
Size: 722 5171
ScaI AGT'ACT
Cuts at: 4141 5853 4141
Size: 1712 4181
SfcI C'TryA_G
Cuts at: 826 1045 2519 3241 4376 5057 5248 826
Size: 219 1474 722 1135 681 191 1471
Fragments arranged by size:
1474 1471 1135 722 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5893
SmaI CCC'GGG
Cuts at: 1872 3220 1872
Size: 1348 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2702 3997 4898 5160 820
Size: 197 926 759 1295 901 262 1553
Fragments arranged by size:
1553 1295 926 901 759 262 197
SnaBI TAC'GTA
Cuts at: 493 3684 493
Size: 3191 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5893
SphI G_CATG'C
Cuts at: 1132 2915 3251 1132
Size: 1783 336 3774
Fragments arranged by size:
3774 1783 336
SspI AAT'ATT
Cuts at: 5 52 3817 5
Size: 47 3765 2081
Fragments arranged by size:
3765 2081 47
StuI AGG'CCT
Cuts at: 1517 2255 2473 1517
Size: 738 218 4937
Fragments arranged by size:
4937 738 218
StyI C'CwwG_G
Cuts at: 513 1065 2995 3332 513
Size: 552 1930 337 3074
Fragments arranged by size:
3074 1930 552 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2251 3920 3937 4090 4275 4720 325
Size: 1255 671 1669 17 153 185 445 1498
Fragments arranged by size:
1669 1498 1255 671 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2823 3073 4139
Size: 274 80 33 51 1443 846 250 1066
Cuts at: 4139 5851 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1066 846 274 250 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5853 5885 5853
Size: 32 5861
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5893
XbaI T'CTAG_A
Cuts at: 3229 3229
Size: 5893
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5893
XmaI C'CCGG_G
Cuts at: 1870 3218 1870
Size: 1348 4545
XmnI GAAnn'nnTTC
Cuts at: 4022 4022
Size: 5893
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3701 276
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SexAI SfiI SfoI SrfI
SwaI XcmI