Restriction map for
pFN21AB7660
(Circular) (Six-base) MAPSORT of: pFN21AB7660.seq Check: 214 from: 1 to: 5890
pFN21AB7660 5890 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2755 2755
Size: 5890
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3700 278
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2718 3233 1937
Size: 781 515 4594
Fragments arranged by size:
4594 781 515
Acc65I G'GTAC_C
Cuts at: 3211 3211
Size: 5890
AclI AA'CG_TT
Cuts at: 1563 4017 4390 1563
Size: 2454 373 3063
Fragments arranged by size:
3063 2454 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3056 3953 5337 5605 1632
Size: 1424 897 1384 268 1917
Fragments arranged by size:
1917 1424 1384 897 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5693
AflIII A'CryG_T
Cuts at: 1203 2276 4789 5766 1203
Size: 1073 2513 977 1327
Fragments arranged by size:
2513 1327 1073 977
AgeI A'CCGG_T
Cuts at: 2603 4721 2603
Size: 2118 3772
AhdI GACnn_n'nnGTC
Cuts at: 4619 4619
Size: 5890
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5890
AlwNI CAG_nnn'CTG
Cuts at: 2597 3633 5205 2597
Size: 1036 1572 3282
Fragments arranged by size:
3282 1572 1036
ApaI G_GGCC'C
Cuts at: 2069 2121 2206 2447 3165 3183 2069
Size: 52 85 241 718 18 4776
Fragments arranged by size:
4776 718 241 85 52 18
ApaLI G'TGCA_C
Cuts at: 3947 5103 3947
Size: 1156 4734
ApoI r'AATT_y
Cuts at: 1493 2337 3199 3425 3577 5686 1493
Size: 844 862 226 152 2109 1697
Fragments arranged by size:
2109 1697 862 844 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4444 160
Size: 872 3412 1606
Fragments arranged by size:
3412 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
AvaI C'yCGr_G
Cuts at: 1870 1943 2625 3142 3215 1870
Size: 73 682 517 73 4545
Fragments arranged by size:
4545 682 517 73 73
BamHI G'GATC_C
Cuts at: 2089 3220 3587 2089
Size: 1131 367 4392
Fragments arranged by size:
4392 1131 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2171 2357 2411 2527 2767
Size: 325 217 627 384 186 54 116 240
Cuts at: 2767 3211 4666 618
Size: 444 1455 1842
Fragments arranged by size:
1842 1455 627 444 384 325 240 217
186 116 54
BanII G_rGCy'C
Cuts at: 1391 2069 2078 2121 2148 2206 2310 2447 2678
Size: 678 9 43 27 58 104 137 231
Cuts at: 2678 3165 3183 3209 1391
Size: 487 18 26 4072
Fragments arranged by size:
4072 678 487 231 137 104 58 43
27 26 18 9
BbeI G_GCGC'C
Cuts at: 2361 2415 2361
Size: 54 5836
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4919
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4081 4115 1903
Size: 34 2144 34 3678
Fragments arranged by size:
3678 2144 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3782 4992 3782
Size: 1210 4680
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5890
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2952 3178 4501
Size: 107 122 71 631 605 1280 226 1323
Cuts at: 4501 136
Size: 1525
Fragments arranged by size:
1525 1323 1280 631 605 226 122 107
71
BglII A'GATC_T
Cuts at: 2369 5885 2369
Size: 3516 2374
BlpI GC'TnA_GC
Cuts at: 3129 3307 3129
Size: 178 5712
Bme1580I G_kGCm'C
Cuts at: 1790 2069 2121 2174 2206 2447 2532 2621 2772
Size: 279 52 53 32 241 85 89 151
Cuts at: 2772 3165 3183 3951 5107 1790
Size: 393 18 768 1156 2573
Fragments arranged by size:
2573 1156 768 393 279 241 151 89
85 53 52 32 18
BmgBI CAC'GTC
Cuts at: 1318 2277 1318
Size: 959 4931
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2514 2522 3621
Size: 416 53 83 399 24 1097 8 1099
Cuts at: 3621 4579 442
Size: 958 1753
Fragments arranged by size:
1753 1099 1097 958 416 399 83 53
24 8
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 2351 2733 2826 3098 4550 1239
Size: 1085 27 382 93 272 1452 2579
Fragments arranged by size:
2579 1452 1085 382 272 93 27
Bpu10I CC'TnA_GC
Cuts at: 2677 2677
Size: 5890
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4015 4880 5178 4015
Size: 865 298 4727
Fragments arranged by size:
4727 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2292 2594 4553 882
Size: 617 793 302 1959 2219
Fragments arranged by size:
2219 1959 793 617 302
BsaAI yAC'GTr
Cuts at: 493 1204 3681 493
Size: 711 2477 2702
Fragments arranged by size:
2702 2477 711
BsaBI GATnn'nnATC
Cuts at: 5884 5884
Size: 5890
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2358 2412 3697 4079
Size: 53 83 186 936 825 54 1285 382
Cuts at: 4079 4714 275
Size: 635 1451
Fragments arranged by size:
1451 1285 936 825 635 382 186 83
54 53
BsaWI w'CCGG_w
Cuts at: 2603 2939 4323 4721 4995 5142 5553 2603
Size: 336 1384 398 274 147 411 2940
Fragments arranged by size:
2940 1384 411 398 336 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5665
Fragments arranged by size:
5665 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2560 3035 3130 1186
Size: 1374 475 95 3946
Fragments arranged by size:
3946 1374 475 95
BseYI C'CCAG_C
Cuts at: 2147 2531 2577 2669 2789 2797 5093 5589 2147
Size: 384 46 92 120 8 2296 496 2448
Fragments arranged by size:
2448 2296 496 384 120 92 46 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5317
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2269 3340 4101 5129 1540
Size: 275 186 268 1071 761 1028 2301
Fragments arranged by size:
2301 1071 1028 761 275 268 186
BsiHKAI G_wGCw'C
Cuts at: 2749 3951 4036 5107 2749
Size: 1202 85 1071 3532
Fragments arranged by size:
3532 1202 1071 85
BsmI GAATG_Cn'
Cuts at: 3401 3494 3401
Size: 93 5797
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2078 2121 2148 2174 2206 2310
Size: 399 279 9 43 27 26 32 104
Cuts at: 2310 2447 2532 2621 2678 2749 2772 3165 3183
Size: 137 85 89 57 71 23 393 18
Cuts at: 3183 3209 3951 4036 5107 1391
Size: 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 393 279 137 104
89 85 85 71 57 43 32 27
26 26 23 18 9
BspEI T'CCGG_A
Cuts at: 2939 5553 2939
Size: 2614 3276
BspHI T'CATG_A
Cuts at: 3779 5509 3779
Size: 1730 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2755 3245 844
Size: 1911 490 3489
Fragments arranged by size:
3489 1911 490
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 2496 3064 3777 1394
Size: 429 187 486 568 713 3507
Fragments arranged by size:
3507 713 568 486 429 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 4385 4559 65
Size: 1183 3137 174 1396
Fragments arranged by size:
3137 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2603 4534 4721 1700
Size: 110 793 1931 187 2869
Fragments arranged by size:
2869 1931 793 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5890
BssHII G'CGCG_C
Cuts at: 1925 3100 1925
Size: 1175 4715
BssSI C'ACGA_G
Cuts at: 3950 4962 3950
Size: 1012 4878
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5890
BstBI TT'CG_AA
Cuts at: 4694 4694
Size: 5890
BstEII G'GTnAC_C
Cuts at: 1184 2909 1184
Size: 1725 4165
BstXI CCAn_nnnn'nTGG
Cuts at: 2986 2986
Size: 5890
BstYI r'GATC_y
Cuts at: 1972 2089 2369 3045 3220 3587 3972 3989 5441
Size: 117 280 676 175 367 385 17 1452
Cuts at: 5441 5527 5539 5885 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 676 385 367 346 280 175
117 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2350 2487 2350
Size: 137 5753
BtgI C'CryG_G
Cuts at: 513 1065 2052 2239 2263 2979 3152 513
Size: 552 987 187 24 716 173 3251
Fragments arranged by size:
3251 987 716 552 187 173 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5151
Fragments arranged by size:
5151 683 56
BtsI GCAGTG_nn'
Cuts at: 3406 4200 4220 3406
Size: 794 20 5076
Fragments arranged by size:
5076 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5855
DraI TTT'AAA
Cuts at: 3194 3194
Size: 5890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4897 809
Size: 507 3581 1802
Fragments arranged by size:
3581 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2394 2898 3108
Size: 54 1425 50 275 18 564 504 210
Cuts at: 3108 3337 4226 5855 8
Size: 229 889 1629 43
Fragments arranged by size:
1629 1425 889 564 504 275 229 210
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3337 1537
Size: 275 1525 4090
Fragments arranged by size:
4090 1525 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2748 3820 1353
Size: 665 730 1072 3423
Fragments arranged by size:
3423 1072 730 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2337 3074 4477 4851 4997 387
Size: 1064 138 748 737 1403 374 146 1280
Fragments arranged by size:
1403 1280 1064 748 737 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2351 2733 2826 3056 3098 3953
Size: 393 692 27 382 93 230 42 855
Cuts at: 3953 4550 5337 5605 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 855 787 692 597 393 382 268
230 93 42 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2438 2650 2438
Size: 212 5678
EcoO109I rG'GnC_Cy
Cuts at: 2065 2066 2118 2202 2203 2346 2443 2444 2688
Size: 1 52 84 1 143 97 1 244
Cuts at: 2688 3148 3162 3180 2065
Size: 460 14 18 4775
Fragments arranged by size:
4775 460 244 143 97 84 52 18
14 1 1 1
EcoRI G'AATT_C
Cuts at: 2337 3199 3577 2337
Size: 862 378 4650
Fragments arranged by size:
4650 862 378
FspI TGC'GCA
Cuts at: 4396 4396
Size: 5890
HaeII r_GCGC'y
Cuts at: 1678 2361 2415 2587 5037 1678
Size: 683 54 172 2450 2531
Fragments arranged by size:
2531 2450 683 172 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2921 2953 4531 4563 4605
Size: 32 135 32 1349 32 1578 32 42
Cuts at: 4605 4637 1373
Size: 32 2626
Fragments arranged by size:
2626 1578 1349 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3234 3480 669
Size: 988 281 1296 246 3079
Fragments arranged by size:
3079 1296 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5890
HpaI GTT'AAC
Cuts at: 3480 3480
Size: 5890
KasI G'GCGC_C
Cuts at: 2357 2411 2357
Size: 54 5836
KpnI G_GTAC'C
Cuts at: 3215 3215
Size: 5890
MfeI C'AATT_G
Cuts at: 3489 3489
Size: 5890
MluI A'CGCG_T
Cuts at: 5766 5766
Size: 5890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2367 5004 5188 1305
Size: 1062 2637 184 2007
Fragments arranged by size:
2637 2007 1062 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2396 2900 3110 10
Size: 54 1425 343 564 504 210 2790
Fragments arranged by size:
2790 1425 564 504 343 210 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3848 4207 4366 518
Size: 648 2682 359 159 2042
Fragments arranged by size:
2682 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2265 2521 3124 3133 3306 3983 5131 5376
Size: 659 256 603 9 173 677 1148 245
Cuts at: 5376 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 659 603 256 245 173
9
NarI GG'CG_CC
Cuts at: 2358 2412 2358
Size: 54 5836
NcoI C'CATG_G
Cuts at: 513 1065 2979 513
Size: 552 1914 3424
Fragments arranged by size:
3424 1914 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5890
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5890
NotI GC'GGCC_GC
Cuts at: 3337 3337
Size: 5890
NspI r_CATG'y
Cuts at: 1132 1229 2725 3248 4793 1132
Size: 97 1496 523 1545 2229
Fragments arranged by size:
2229 1545 1496 523 97
PasI CC'CwG_GG
Cuts at: 1348 2069 2078 1348
Size: 721 9 5160
Fragments arranged by size:
5160 721 9
PciI A'CATG_T
Cuts at: 4789 4789
Size: 5890
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2198 2245 2454 2524 3158 1108
Size: 1090 47 209 70 634 3840
Fragments arranged by size:
3840 1090 634 209 70 47
PfoI T'CCnGG_A
Cuts at: 2328 2479 2702 3015 3048 2328
Size: 151 223 313 33 5170
Fragments arranged by size:
5170 313 223 151 33
PmeI GTTT'AAAC
Cuts at: 3194 3194
Size: 5890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 5472 5504 3959
Size: 32 1481 32 4345
Fragments arranged by size:
4345 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2688 3148 2688
Size: 460 5430
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5890
PsiI TTA'TAA
Cuts at: 3460 3460
Size: 5890
PspOMI G'GGCC_C
Cuts at: 2065 2117 2202 2443 3161 3179 2065
Size: 52 85 241 718 18 4776
Fragments arranged by size:
4776 718 241 85 52 18
PstI C_TGCA'G
Cuts at: 830 3242 830
Size: 2412 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5890
PvuII CAG'CTG
Cuts at: 2521 3124 3133 2521
Size: 603 9 5278
Fragments arranged by size:
5278 603 9
RsrII CG'GwC_CG
Cuts at: 3013 3013
Size: 5890
SacII CC_GC'GG
Cuts at: 2266 2266
Size: 5890
SalI G'TCGA_C
Cuts at: 1936 3232 1936
Size: 1296 4594
SanDI GG'GwC_CC
Cuts at: 3148 3148
Size: 5890
SbfI CC_TGCA'GG
Cuts at: 3242 3242
Size: 5890
ScaI AGT'ACT
Cuts at: 4138 5850 4138
Size: 1712 4178
SfcI C'TryA_G
Cuts at: 826 1045 2715 3238 4373 5054 5245 826
Size: 219 1670 523 1135 681 191 1471
Fragments arranged by size:
1670 1471 1135 681 523 219 191
SfoI GGC'GCC
Cuts at: 2359 2413 2359
Size: 54 5836
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5890
SmaI CCC'GGG
Cuts at: 1872 2627 3217 1872
Size: 755 590 4545
Fragments arranged by size:
4545 755 590
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3994 4895 5157 820
Size: 197 926 2051 901 262 1553
Fragments arranged by size:
2051 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3681 493
Size: 3188 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5890
SphI G_CATG'C
Cuts at: 1132 3248 1132
Size: 2116 3774
SspI AAT'ATT
Cuts at: 5 52 3814 5
Size: 47 3762 2081
Fragments arranged by size:
3762 2081 47
StuI AGG'CCT
Cuts at: 1517 2433 1517
Size: 916 4974
StyI C'CwwG_G
Cuts at: 513 1065 2541 2979 3329 513
Size: 552 1476 438 350 3074
Fragments arranged by size:
3074 1476 552 438 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2732 2828 3917 3934 4087 4272 4717
Size: 1255 1152 96 1089 17 153 185 445
Cuts at: 4717 325
Size: 1498
Fragments arranged by size:
1498 1255 1152 1089 445 185 153 96
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4136 5848 96
Size: 274 80 33 51 1443 2159 1712 138
Fragments arranged by size:
2159 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5850 5882 5850
Size: 32 5858
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5890
XbaI T'CTAG_A
Cuts at: 3226 3226
Size: 5890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2389 2404 2389
Size: 15 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5890
XmaI C'CCGG_G
Cuts at: 1870 2625 3215 1870
Size: 755 590 4545
Fragments arranged by size:
4545 755 590
XmnI GAAnn'nnTTC
Cuts at: 4019 4019
Size: 5890
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3698 276
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI SacI SapI SexAI SfiI SrfI SwaI