Restriction map for
pFN21AB7657
(Circular) (Six-base) MAPSORT of: pFN21AB7657.seq Check: 8252 from: 1 to: 5707
pFN21AB7657 5707 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2365 2365
Size: 5707
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2246 2619 3770 5154 5422 1632
Size: 614 373 1151 1384 268 1917
Fragments arranged by size:
1917 1384 1151 614 373 268
AfeI AGC'GCT
Cuts at: 2080 2080
Size: 5707
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 4606 5583 1203
Size: 3403 977 1327
Fragments arranged by size:
3403 1327 977
AgeI A'CCGG_T
Cuts at: 2060 2401 4538 2060
Size: 341 2137 3229
Fragments arranged by size:
3229 2137 341
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5707
AlwNI CAG_nnn'CTG
Cuts at: 2687 3450 5022 2687
Size: 763 1572 3372
Fragments arranged by size:
3372 1572 763
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 3016 3242 3394 5503 1493
Size: 1523 226 152 2109 1697
Fragments arranged by size:
2109 1697 1523 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 2157 2226 2672 3032 1870
Size: 73 214 69 446 360 4545
Fragments arranged by size:
4545 446 360 214 73 69
BamHI G'GATC_C
Cuts at: 3037 3404 3037
Size: 367 5340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2274 2866 2965 3028 4483
Size: 325 217 627 487 592 99 63 1455
Cuts at: 4483 618
Size: 1842
Fragments arranged by size:
1842 1455 627 592 487 325 217 99
63
BanII G_rGCy'C
Cuts at: 1391 2679 3026 1391
Size: 1288 347 4072
Fragments arranged by size:
4072 1288 347
BbeI G_GCGC'C
Cuts at: 2278 2278
Size: 5707
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2188 2493 2610 928
Size: 971 289 305 117 4025
Fragments arranged by size:
4025 971 305 289 117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 1903
Size: 34 1961 34 3678
Fragments arranged by size:
3678 1961 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3599 4809 3599
Size: 1210 4497
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2231 4318 136
Size: 107 122 71 631 605 559 2087 1525
Fragments arranged by size:
2087 1525 631 605 559 122 107 71
BglII A'GATC_T
Cuts at: 2628 5702 2628
Size: 3074 2633
BlpI GC'TnA_GC
Cuts at: 3124 3124
Size: 5707
Bme1580I G_kGCm'C
Cuts at: 1790 2970 3768 4924 1790
Size: 1180 798 1156 2573
Fragments arranged by size:
2573 1180 1156 798
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5707
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3438 4396 442
Size: 416 53 83 399 24 2021 958 1753
Fragments arranged by size:
2021 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2095 2186 2496 2838 2993 4367 1239
Size: 856 91 310 342 155 1374 2579
Fragments arranged by size:
2579 1374 856 342 310 155 91
Bpu10I CC'TnA_GC
Cuts at: 2465 2465
Size: 5707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 3832 4697 4995 2195
Size: 1637 865 298 2907
Fragments arranged by size:
2907 1637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2870 4370 882
Size: 617 1371 1500 2219
Fragments arranged by size:
2219 1500 1371 617
BsaAI yAC'GTr
Cuts at: 493 1204 3498 493
Size: 711 2294 2702
Fragments arranged by size:
2702 2294 711
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2142 2275 3514 3896
Size: 53 83 186 936 609 133 1239 382
Cuts at: 3896 4531 275
Size: 635 1451
Fragments arranged by size:
1451 1239 936 635 609 382 186 133
83 53
BsaWI w'CCGG_w
Cuts at: 2060 2401 4140 4538 4812 4959 5370 2060
Size: 341 1739 398 274 147 411 2397
Fragments arranged by size:
2397 1739 411 398 341 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5482
Fragments arranged by size:
5482 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2744 2888 1186
Size: 1558 144 4005
Fragments arranged by size:
4005 1558 144
BseYI C'CCAG_C
Cuts at: 2293 2802 2884 2936 4910 5406 2293
Size: 509 82 52 1974 496 2594
Fragments arranged by size:
2594 1974 509 496 82 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2399 2508 2734 1163
Size: 573 663 109 226 4136
Fragments arranged by size:
4136 663 573 226 109
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3157 3918 4946 1540
Size: 275 186 1156 761 1028 2301
Fragments arranged by size:
2301 1156 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2382 2679 2907 3768 3853 4924 2382
Size: 297 228 861 85 1071 3165
Fragments arranged by size:
3165 1071 861 297 228 85
BsmI GAATG_Cn'
Cuts at: 3218 3311 3218
Size: 93 5614
BsmBI CGTCTCn'nnnn_
Cuts at: 2771 2771
Size: 5707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2382 2679 2907 2970 3026 3768 3853
Size: 399 592 297 228 63 56 742 85
Cuts at: 3853 4924 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 592 399 297 228 85
63 56
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2365 2718 3062 844
Size: 1521 353 344 3489
Fragments arranged by size:
3489 1521 353 344
BsrBI CCG'CTC
Cuts at: 1394 1823 2030 3594 1394
Size: 429 207 1564 3507
Fragments arranged by size:
3507 1564 429 207
BsrDI GCAATG_nn'
Cuts at: 65 1248 4202 4376 65
Size: 1183 2954 174 1396
Fragments arranged by size:
2954 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 2401 4351 4538 1700
Size: 110 250 341 1950 187 2869
Fragments arranged by size:
2869 1950 341 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5707
BssHII G'CGCG_C
Cuts at: 1925 2039 1925
Size: 114 5593
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2101 794
Size: 1307 4400
BstBI TT'CG_AA
Cuts at: 4511 4511
Size: 5707
BstEII G'GTnAC_C
Cuts at: 1184 2056 2404 1184
Size: 872 348 4487
Fragments arranged by size:
4487 872 348
BstXI CCAn_nnnn'nTGG
Cuts at: 2152 2152
Size: 5707
BstYI r'GATC_y
Cuts at: 1972 2187 2317 2628 2940 3037 3404 3789 3806
Size: 215 130 311 312 97 367 385 17
Cuts at: 3806 5258 5344 5356 5702 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 312 311 215
130 97 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2394 2446 513
Size: 552 1329 52 3774
Fragments arranged by size:
3774 1329 552 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2371 487
Size: 683 56 1145 3823
Fragments arranged by size:
3823 1145 683 56
BtsI GCAGTG_nn'
Cuts at: 2367 3223 4017 4037 2367
Size: 856 794 20 4037
Fragments arranged by size:
4037 856 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4714 809
Size: 507 3398 1802
Fragments arranged by size:
3398 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2391 2662 3154
Size: 54 1425 50 275 18 561 271 492
Cuts at: 3154 4043 5672 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 561 492 275 271 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3637 1353
Size: 2284 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 4668 4814 387
Size: 1064 138 2705 374 146 1280
Fragments arranged by size:
2705 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2677 2677
Size: 5707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 2186 2246 2496 2619 2838 2993
Size: 393 463 91 60 250 123 219 155
Cuts at: 2993 3770 4367 5154 5422 1239
Size: 777 597 787 268 1524
Fragments arranged by size:
1524 787 777 597 463 393 268 250
219 155 123 91 60
EcoO109I rG'GnC_Cy
Cuts at: 2831 2831
Size: 5707
EcoRI G'AATT_C
Cuts at: 3016 3394 3016
Size: 378 5329
FspI TGC'GCA
Cuts at: 4213 4213
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 2082 2278 2472 4854 1678
Size: 404 196 194 2382 2531
Fragments arranged by size:
2531 2382 404 196 194
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4348 4380 4422 4454 1373
Size: 32 135 32 2776 32 42 32 2626
Fragments arranged by size:
2776 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3051 3297 669
Size: 988 281 1113 246 3079
Fragments arranged by size:
3079 1113 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5707
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KasI G'GCGC_C
Cuts at: 2274 2274
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 3306 3306
Size: 5707
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4821 5005 1305
Size: 3516 184 2007
Fragments arranged by size:
3516 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3885
Fragments arranged by size:
3885 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2253 3665 4024 4183 518
Size: 648 1087 1412 359 159 2042
Fragments arranged by size:
2042 1412 1087 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2687 2696 2816 2936 3123 3800 4948 5193
Size: 1081 9 120 120 187 677 1148 245
Cuts at: 5193 1606
Size: 2120
Fragments arranged by size:
2120 1148 1081 677 245 187 120 120
9
NarI GG'CG_CC
Cuts at: 2275 2275
Size: 5707
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5155
NdeI CA'TA_TG
Cuts at: 387 2769 387
Size: 2382 3325
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NruI TCG'CGA
Cuts at: 2085 2085
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 3065 4610 1132
Size: 97 1836 1545 2229
Fragments arranged by size:
2229 1836 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5707
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5707
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PstI C_TGCA'G
Cuts at: 830 2115 2839 3059 830
Size: 1285 724 220 3478
Fragments arranged by size:
3478 1285 724 220
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
PvuII CAG'CTG
Cuts at: 2687 2936 2687
Size: 249 5458
SacI G_AGCT'C
Cuts at: 2679 2679
Size: 5707
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SbfI CC_TGCA'GG
Cuts at: 2115 3059 2115
Size: 944 4763
ScaI AGT'ACT
Cuts at: 2639 3955 5667 2639
Size: 1316 1712 2679
Fragments arranged by size:
2679 1712 1316
SfcI C'TryA_G
Cuts at: 826 1045 2111 2835 3055 4190 4871 5062 826
Size: 219 1066 724 220 1135 681 191 1471
Fragments arranged by size:
1471 1135 1066 724 681 220 219 191
SfoI GGC'GCC
Cuts at: 2276 2276
Size: 5707
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 2159 2228 3034 1872
Size: 287 69 806 4545
Fragments arranged by size:
4545 806 287 69
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2210 3811 4712 4974 820
Size: 197 926 267 1601 901 262 1553
Fragments arranged by size:
1601 1553 926 901 267 262 197
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 3065 1132
Size: 1933 3774
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5707
StyI C'CwwG_G
Cuts at: 513 1065 2270 2862 2924 3146 513
Size: 552 1205 592 62 222 3074
Fragments arranged by size:
3074 1205 592 552 222 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2554 3734 3751 3904 4089 4534 325
Size: 1255 974 1180 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1180 974 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2637 3953 5665
Size: 274 80 33 51 1443 660 1316 1712
Cuts at: 5665 96
Size: 138
Fragments arranged by size:
1712 1443 1316 660 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5667 5699 5667
Size: 32 5675
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5707
XbaI T'CTAG_A
Cuts at: 3043 3043
Size: 5707
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 2157 2226 3032 1870
Size: 287 69 806 4545
Fragments arranged by size:
4545 806 287 69
XmnI GAAnn'nnTTC
Cuts at: 2957 3836 2957
Size: 879 4828
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NruI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI