Restriction map for
pFN21AB7650
(Circular) (Six-base) MAPSORT of: pFN21AB7650.seq Check: 6958 from: 1 to: 5881
pFN21AB7650 5881 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3101 3101
Size: 5881
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3691 278
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2740 3224 1937
Size: 803 484 4594
Fragments arranged by size:
4594 803 484
Acc65I G'GTAC_C
Cuts at: 3202 3202
Size: 5881
AclI AA'CG_TT
Cuts at: 1563 4008 4381 1563
Size: 2445 373 3063
Fragments arranged by size:
3063 2445 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2815 3195 3944 5328 5596 1632
Size: 1183 380 749 1384 268 1917
Fragments arranged by size:
1917 1384 1183 749 380 268
AfeI AGC'GCT
Cuts at: 2063 2063
Size: 5881
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5684
AflIII A'CryG_T
Cuts at: 1203 4780 5757 1203
Size: 3577 977 1327
Fragments arranged by size:
3577 1327 977
AgeI A'CCGG_T
Cuts at: 4712 4712
Size: 5881
AhdI GACnn_n'nnGTC
Cuts at: 4610 4610
Size: 5881
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5881
AlwNI CAG_nnn'CTG
Cuts at: 2971 3168 3624 5196 2971
Size: 197 456 1572 3656
Fragments arranged by size:
3656 1572 456 197
ApaLI G'TGCA_C
Cuts at: 2220 3938 5094 2220
Size: 1718 1156 3007
Fragments arranged by size:
3007 1718 1156
ApoI r'AATT_y
Cuts at: 1493 2423 3190 3416 3568 5677 1493
Size: 930 767 226 152 2109 1697
Fragments arranged by size:
2109 1697 930 767 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4435 160
Size: 872 3403 1606
Fragments arranged by size:
3403 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
AvaI C'yCGr_G
Cuts at: 1870 1943 2075 3206 1870
Size: 73 132 1131 4545
Fragments arranged by size:
4545 1131 132 73
BamHI G'GATC_C
Cuts at: 3211 3578 3211
Size: 367 5514
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3131 3202 4657 618
Size: 325 217 627 1344 71 1455 1842
Fragments arranged by size:
1842 1455 1344 627 325 217 71
BanII G_rGCy'C
Cuts at: 1391 3200 1391
Size: 1809 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4910
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2161 2195 4072 4106 1903
Size: 34 224 34 1877 34 3678
Fragments arranged by size:
3678 1877 224 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2319 3773 4983 2319
Size: 1454 1210 3217
Fragments arranged by size:
3217 1454 1210
BclI T'GATC_A
Cuts at: 1548 2649 2730 3021 1548
Size: 1101 81 291 4408
Fragments arranged by size:
4408 1101 291 81
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2780 4492 136
Size: 107 122 71 631 605 1108 1712 1525
Fragments arranged by size:
1712 1525 1108 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5876 5876
Size: 5881
BlpI GC'TnA_GC
Cuts at: 3298 3298
Size: 5881
Bme1580I G_kGCm'C
Cuts at: 1790 2224 3942 5098 1790
Size: 434 1718 1156 2573
Fragments arranged by size:
2573 1718 1156 434
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5881
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3178 3612 4570
Size: 416 53 83 399 24 1761 434 958
Cuts at: 4570 442
Size: 1753
Fragments arranged by size:
1761 1753 958 434 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5881
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3085 3085
Size: 5881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 2348 2786 3106 4541 1239
Size: 881 228 438 320 1435 2579
Fragments arranged by size:
2579 1435 881 438 320 228
Bpu10I CC'TnA_GC
Cuts at: 2030 2030
Size: 5881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4006 4871 5169 4006
Size: 865 298 4718
Fragments arranged by size:
4718 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4544 882
Size: 617 3045 2219
Fragments arranged by size:
3045 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3672 493
Size: 711 2468 2702
Fragments arranged by size:
2702 2468 711
BsaBI GATnn'nnATC
Cuts at: 5875 5875
Size: 5881
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3688 4070 4705 275
Size: 53 83 186 936 2155 382 635 1451
Fragments arranged by size:
2155 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4314 4712 4986 5133 5544 4314
Size: 398 274 147 411 4651
Fragments arranged by size:
4651 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2321 2351 3104 3134 1178
Size: 30 165 30 918 30 753 30 3925
Fragments arranged by size:
3925 918 753 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2930 1186
Size: 1744 4137
BseYI C'CCAG_C
Cuts at: 2088 5084 5580 2088
Size: 2996 496 2389
Fragments arranged by size:
2996 2389 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3079 1163
Size: 573 1343 3965
Fragments arranged by size:
3965 1343 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2112 2137 3331 4092 5120 1540
Size: 275 186 111 25 1194 761 1028 2301
Fragments arranged by size:
2301 1194 1028 761 275 186 111 25
BsiHKAI G_wGCw'C
Cuts at: 2224 3942 4027 5098 2224
Size: 1718 85 1071 3007
Fragments arranged by size:
3007 1718 1071 85
BsmI GAATG_Cn'
Cuts at: 3392 3485 3392
Size: 93 5788
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2224 3200 3942 4027 5098 1391
Size: 399 434 976 742 85 1071 2174
Fragments arranged by size:
2174 1071 976 742 434 399 85
BspEI T'CCGG_A
Cuts at: 5544 5544
Size: 5881
BspHI T'CATG_A
Cuts at: 3770 5500 3770
Size: 1730 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3101 3236 844
Size: 2257 135 3489
Fragments arranged by size:
3489 2257 135
BsrBI CCG'CTC
Cuts at: 1394 1823 3768 1394
Size: 429 1945 3507
Fragments arranged by size:
3507 1945 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3133 4376 4550 65
Size: 1183 1885 1243 174 1396
Fragments arranged by size:
1885 1396 1243 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2107 2136 4525 4712 1700
Size: 110 297 29 2389 187 2869
Fragments arranged by size:
2869 2389 297 187 110 29
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5881
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5881
BssSI C'ACGA_G
Cuts at: 3941 4953 3941
Size: 1012 4869
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3005 794
Size: 2211 3670
BstBI TT'CG_AA
Cuts at: 4685 4685
Size: 5881
BstEII G'GTnAC_C
Cuts at: 1184 2123 2671 1184
Size: 939 548 4394
Fragments arranged by size:
4394 939 548
BstYI r'GATC_y
Cuts at: 1972 3211 3578 3963 3980 5432 5518 5530 5876
Size: 1239 367 385 17 1452 86 12 346
Cuts at: 5876 1972
Size: 1977
Fragments arranged by size:
1977 1452 1239 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2131 513
Size: 552 939 127 4263
Fragments arranged by size:
4263 939 552 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5142
Fragments arranged by size:
5142 683 56
BtsI GCAGTG_nn'
Cuts at: 3058 3397 4191 4211 3058
Size: 339 794 20 4728
Fragments arranged by size:
4728 794 339 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5881
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5846
DraI TTT'AAA
Cuts at: 3185 3185
Size: 5881
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4888 809
Size: 507 3572 1802
Fragments arranged by size:
3572 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2134 2781
Size: 54 1425 50 275 18 279 25 647
Cuts at: 2781 3328 4217 5846 8
Size: 547 889 1629 43
Fragments arranged by size:
1629 1425 889 647 547 279 275 54
50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 2109 2134 3328 1537
Size: 275 297 25 1194 4090
Fragments arranged by size:
4090 1194 297 275 25
EarI CTCTTCn'nnn_
Cuts at: 1353 2198 3171 3811 1353
Size: 845 973 640 3423
Fragments arranged by size:
3423 973 845 640
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4468 4842 4988 387
Size: 1064 138 2879 374 146 1280
Fragments arranged by size:
2879 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2348 2786 2815 3106 3195 3944
Size: 393 488 228 438 29 291 89 749
Cuts at: 3944 4541 5328 5596 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 749 597 488 438 393 291
268 228 89 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2282 3022 2282
Size: 740 5141
EcoO109I rG'GnC_Cy
Cuts at: 2024 2332 2024
Size: 308 5573
EcoRI G'AATT_C
Cuts at: 3190 3568 3190
Size: 378 5503
FseI GG_CCGG'CC
Cuts at: 2111 2111
Size: 5881
FspI TGC'GCA
Cuts at: 4387 4387
Size: 5881
HaeII r_GCGC'y
Cuts at: 1678 2065 5028 1678
Size: 387 2963 2531
Fragments arranged by size:
2963 2531 387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 2722 2754 2847
Size: 32 135 32 749 32 369 32 93
Cuts at: 2847 2879 3104 3136 4522 4554 4596 4628 1373
Size: 32 225 32 1386 32 42 32 2626
Fragments arranged by size:
2626 1386 749 369 225 135 93 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3225 3471 669
Size: 988 281 1287 246 3079
Fragments arranged by size:
3079 1287 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2032 748
Size: 1284 4597
HpaI GTT'AAC
Cuts at: 3471 3471
Size: 5881
KpnI G_GTAC'C
Cuts at: 3206 3206
Size: 5881
MfeI C'AATT_G
Cuts at: 3480 3480
Size: 5881
MluI A'CGCG_T
Cuts at: 5757 5757
Size: 5881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2542 4995 5179 1305
Size: 1237 2453 184 2007
Fragments arranged by size:
2453 2007 1237 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2783 10
Size: 54 1425 343 951 3108
Fragments arranged by size:
3108 1425 951 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2657 3839 4198 4357 518
Size: 648 1491 1182 359 159 2042
Fragments arranged by size:
2042 1491 1182 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2133 2161 2347 2803 3297 3974 5122 5367
Size: 527 28 186 456 494 677 1148 245
Cuts at: 5367 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 527 494 456 245 186
28
NaeI GCC'GGC
Cuts at: 2109 2138 2109
Size: 29 5852
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4390
Fragments arranged by size:
4390 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5881
NgoMIV G'CCGG_C
Cuts at: 2107 2136 2107
Size: 29 5852
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5881
NotI GC'GGCC_GC
Cuts at: 3328 3328
Size: 5881
NspI r_CATG'y
Cuts at: 1132 1229 2440 2978 3239 4784 1132
Size: 97 1211 538 261 1545 2229
Fragments arranged by size:
2229 1545 1211 538 261 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5881
PciI A'CATG_T
Cuts at: 4780 4780
Size: 5881
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5881
PmeI GTTT'AAAC
Cuts at: 3185 3185
Size: 5881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3950 3982 5463 5495 3950
Size: 32 1481 32 4336
Fragments arranged by size:
4336 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2024 2024
Size: 5881
PshAI GACnn'nnGTC
Cuts at: 1613 2247 1613
Size: 634 5247
PsiI TTA'TAA
Cuts at: 3451 3451
Size: 5881
PstI C_TGCA'G
Cuts at: 830 3233 830
Size: 2403 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5881
PvuII CAG'CTG
Cuts at: 2347 2347
Size: 5881
SacII CC_GC'GG
Cuts at: 2134 2134
Size: 5881
SalI G'TCGA_C
Cuts at: 1936 3223 1936
Size: 1287 4594
SanDI GG'GwC_CC
Cuts at: 2024 2024
Size: 5881
SapI GCTCTTCn'nnn_
Cuts at: 2198 2198
Size: 5881
SbfI CC_TGCA'GG
Cuts at: 3233 3233
Size: 5881
ScaI AGT'ACT
Cuts at: 4129 5841 4129
Size: 1712 4169
SfcI C'TryA_G
Cuts at: 826 1045 2963 3229 4364 5045 5236 826
Size: 219 1918 266 1135 681 191 1471
Fragments arranged by size:
1918 1471 1135 681 266 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5881
SmaI CCC'GGG
Cuts at: 1872 3208 1872
Size: 1336 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3985 4886 5148 820
Size: 197 926 2042 901 262 1553
Fragments arranged by size:
2042 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3672 493
Size: 3179 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5881
SphI G_CATG'C
Cuts at: 1132 3239 1132
Size: 2107 3774
SspI AAT'ATT
Cuts at: 5 52 3805 5
Size: 47 3753 2081
Fragments arranged by size:
3753 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5881
StyI C'CwwG_G
Cuts at: 513 1065 2004 2501 3320 513
Size: 552 939 497 819 3074
Fragments arranged by size:
3074 939 819 552 497
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3908 3925 4078 4263 4708 325
Size: 1255 2328 17 153 185 445 1498
Fragments arranged by size:
2328 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4127 5839 96
Size: 274 80 33 51 1443 2150 1712 138
Fragments arranged by size:
2150 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3088 3120 5841 5873 3088
Size: 32 2721 32 3096
Fragments arranged by size:
3096 2721 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5881
XbaI T'CTAG_A
Cuts at: 3217 3217
Size: 5881
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5881
XmaI C'CCGG_G
Cuts at: 1870 3206 1870
Size: 1336 4545
XmnI GAAnn'nnTTC
Cuts at: 4010 4010
Size: 5881
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3689 276
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SexAI SfiI
SfoI SrfI SwaI XcmI