Restriction map for
pFN21AB7649
(Circular) (Six-base) MAPSORT of: pFN21AB7649.seq Check: 8517 from: 1 to: 5692
pFN21AB7649 5692 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3035 1937
Size: 1098 4594
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2588 2607 2700 3755 5139 5407 1632
Size: 956 19 93 1055 1384 268 1917
Fragments arranged by size:
1917 1384 1055 956 268 93 19
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 4591 5568 1203
Size: 3388 977 1327
Fragments arranged by size:
3388 1327 977
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 2741 1166
Size: 1575 4117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5692
AlwNI CAG_nnn'CTG
Cuts at: 3435 5007 3435
Size: 1572 4120
ApaI G_GGCC'C
Cuts at: 2036 2036
Size: 5692
ApaLI G'TGCA_C
Cuts at: 2860 3749 4905 2860
Size: 889 1156 3647
Fragments arranged by size:
3647 1156 889
ApoI r'AATT_y
Cuts at: 1493 2317 3001 3227 3379 5488 1493
Size: 824 684 226 152 2109 1697
Fragments arranged by size:
2109 1697 824 684 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 2097 2937 3017 1870
Size: 73 92 62 840 80 4545
Fragments arranged by size:
4545 840 92 80 73 62
BamHI G'GATC_C
Cuts at: 2692 3022 3389 2692
Size: 330 367 4995
Fragments arranged by size:
4995 367 330
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2873 3013 4468 618
Size: 325 217 627 1086 140 1455 1842
Fragments arranged by size:
1842 1455 1086 627 325 217 140
BanII G_rGCy'C
Cuts at: 1391 2036 2631 3011 1391
Size: 645 595 380 4072
Fragments arranged by size:
4072 645 595 380
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4721
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2056 2090 3883 3917 1903
Size: 34 119 34 1793 34 3678
Fragments arranged by size:
3678 1793 119 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4794 3584
Size: 1210 4482
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2061 4303 136
Size: 107 122 71 631 605 389 2242 1525
Fragments arranged by size:
2242 1525 631 605 389 122 107 71
BglII A'GATC_T
Cuts at: 2252 5687 2252
Size: 3435 2257
BlpI GC'TnA_GC
Cuts at: 2550 3109 2550
Size: 559 5133
Bme1580I G_kGCm'C
Cuts at: 1790 2036 2864 3753 4909 1790
Size: 246 828 889 1156 2573
Fragments arranged by size:
2573 1156 889 828 246
BmgBI CAC'GTC
Cuts at: 1318 2646 1318
Size: 1328 4364
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2808 3423 4381
Size: 416 53 83 399 24 1391 615 958
Cuts at: 4381 442
Size: 1753
Fragments arranged by size:
1753 1391 958 615 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2012 2386 2513 2693 4352 1239
Size: 773 374 127 180 1659 2579
Fragments arranged by size:
2579 1659 773 374 180 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3817 4682 4980 3817
Size: 865 298 4529
Fragments arranged by size:
4529 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2277 4355 882
Size: 617 778 2078 2219
Fragments arranged by size:
2219 2078 778 617
BsaAI yAC'GTr
Cuts at: 493 1204 3483 493
Size: 711 2279 2702
Fragments arranged by size:
2702 2279 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3499 3881 4516 275
Size: 53 83 186 936 1966 382 635 1451
Fragments arranged by size:
1966 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4125 4523 4797 4944 5355 4125
Size: 398 274 147 411 4462
Fragments arranged by size:
4462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5467
Fragments arranged by size:
5467 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2265 1186
Size: 1079 4613
BseYI C'CCAG_C
Cuts at: 2013 2443 4895 5391 2013
Size: 430 2452 496 2314
Fragments arranged by size:
2452 2314 496 430
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2206 2330 1163
Size: 573 470 124 4525
Fragments arranged by size:
4525 573 470 124
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3142 3903 4931 1540
Size: 275 186 1141 761 1028 2301
Fragments arranged by size:
2301 1141 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2864 3753 3838 4909 2864
Size: 889 85 1071 3647
Fragments arranged by size:
3647 1071 889 85
BsmI GAATG_Cn'
Cuts at: 3203 3296 3203
Size: 93 5599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2631 2864 3011 3753 3838 4909
Size: 399 246 595 233 147 742 85 1071
Cuts at: 4909 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 595 399 246 233 147
85
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3047 844
Size: 2203 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3579 1394
Size: 429 1756 3507
Fragments arranged by size:
3507 1756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4187 4361 65
Size: 1183 2939 174 1396
Fragments arranged by size:
2939 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4336 4523 1700
Size: 110 2526 187 2869
Fragments arranged by size:
2869 2526 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 2057 3752 4764 2057
Size: 1695 1012 2985
Fragments arranged by size:
2985 1695 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5692
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstXI CCAn_nnnn'nTGG
Cuts at: 2518 2518
Size: 5692
BstYI r'GATC_y
Cuts at: 1972 2252 2692 3022 3389 3774 3791 5243 5329
Size: 280 440 330 367 385 17 1452 86
Cuts at: 5329 5341 5687 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 440 385 367 346 330 280
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2707 2958 2707
Size: 251 5441
BtgI C'CryG_G
Cuts at: 513 1065 2042 513
Size: 552 977 4163
Fragments arranged by size:
4163 977 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 3208 4002 4022 3208
Size: 794 20 4878
Fragments arranged by size:
4878 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3139 4028 5657
Size: 54 1425 50 275 18 1309 889 1629
Cuts at: 5657 8
Size: 43
Fragments arranged by size:
1629 1425 1309 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3139 1537
Size: 275 1327 4090
Fragments arranged by size:
4090 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2464 3622 1353
Size: 1111 1158 3423
Fragments arranged by size:
3423 1158 1111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 4279 4653 4799 387
Size: 1064 138 453 2237 374 146 1280
Fragments arranged by size:
2237 1280 1064 453 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2012 2386 2513 2588 2607 2693 2700
Size: 393 380 374 127 75 19 86 7
Cuts at: 2700 3755 4352 5139 5407 1239
Size: 1055 597 787 268 1524
Fragments arranged by size:
1524 1055 787 597 393 380 374 268
127 86 75 19 7
EcoO109I rG'GnC_Cy
Cuts at: 2032 2341 2032
Size: 309 5383
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 2361 4839 1678
Size: 683 2478 2531
Fragments arranged by size:
2531 2478 683
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2136 2168 2567 2599 4333
Size: 32 135 32 564 32 399 32 1734
Cuts at: 4333 4365 4407 4439 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1734 564 399 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3036 3282 669
Size: 988 281 1098 246 3079
Fragments arranged by size:
3079 1098 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2499 4806 4990 1305
Size: 1194 2307 184 2007
Fragments arranged by size:
2307 2007 1194 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3870
Fragments arranged by size:
3870 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2741 3650 4009 4168 518
Size: 648 1575 909 359 159 2042
Fragments arranged by size:
2042 1575 909 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2447 2514 3108 3785 4933 5178 1606
Size: 841 67 594 677 1148 245 2120
Fragments arranged by size:
2120 1148 841 677 594 245 67
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5140
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 2936 3050 4595 1132
Size: 97 1707 114 1545 2229
Fragments arranged by size:
2229 1707 1545 114 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5692
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5692
PfoI T'CCnGG_A
Cuts at: 2107 2670 2107
Size: 563 5129
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2341 2341
Size: 5692
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PspOMI G'GGCC_C
Cuts at: 2032 2032
Size: 5692
PstI C_TGCA'G
Cuts at: 830 3044 830
Size: 2214 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
PvuII CAG'CTG
Cuts at: 2447 2447
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 2244 3040 4175 4856 5047 826
Size: 219 1199 796 1135 681 191 1471
Fragments arranged by size:
1471 1199 1135 796 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 2037 2099 2939 3019 1872
Size: 165 62 840 80 4545
Fragments arranged by size:
4545 840 165 80 62
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3796 4697 4959 820
Size: 197 926 1853 901 262 1553
Fragments arranged by size:
1853 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 2936 3050 1132
Size: 1804 114 3774
Fragments arranged by size:
3774 1804 114
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5692
StyI C'CwwG_G
Cuts at: 513 1065 2988 3131 513
Size: 552 1923 143 3074
Fragments arranged by size:
3074 1923 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2027 3719 3736 3889 4074 4519 325
Size: 1255 447 1692 17 153 185 445 1498
Fragments arranged by size:
1692 1498 1255 447 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2433 3938 5650
Size: 274 80 33 51 1443 456 1505 1712
Cuts at: 5650 96
Size: 138
Fragments arranged by size:
1712 1505 1443 456 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2794 2826 5652 5684 2794
Size: 32 2826 32 2802
Fragments arranged by size:
2826 2802 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2650 2650
Size: 5692
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 2035 2097 2937 3017 1870
Size: 165 62 840 80 4545
Fragments arranged by size:
4545 840 165 80 62
XmnI GAAnn'nnTTC
Cuts at: 3821 3821
Size: 5692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI