Restriction map for
pFN21AB7647
(Circular) (Six-base) MAPSORT of: pFN21AB7647.seq Check: 8513 from: 1 to: 5689
pFN21AB7647 5689 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3032 1937
Size: 1095 4594
Acc65I G'GTAC_C
Cuts at: 2394 3010 2394
Size: 616 5073
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3752 5136 5404 1632
Size: 2120 1384 268 1917
Fragments arranged by size:
2120 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 4588 5565 1203
Size: 3385 977 1327
Fragments arranged by size:
3385 1327 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 2041 2210 4418 2041
Size: 169 2208 3312
Fragments arranged by size:
3312 2208 169
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2708 1184
Size: 1524 4165
AlwNI CAG_nnn'CTG
Cuts at: 3432 5004 3432
Size: 1572 4117
ApaI G_GGCC'C
Cuts at: 2418 2749 2418
Size: 331 5358
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2998 3224 3376 5485 1493
Size: 1505 226 152 2109 1697
Fragments arranged by size:
2109 1697 1505 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2331 3014 1870
Size: 73 388 683 4545
Fragments arranged by size:
4545 683 388 73
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2394 3010 4465 618
Size: 325 217 627 607 616 1455 1842
Fragments arranged by size:
1842 1455 627 616 607 325 217
BanII G_rGCy'C
Cuts at: 1391 2240 2338 2418 2749 3008 1391
Size: 849 98 80 331 259 4072
Fragments arranged by size:
4072 849 331 259 98 80
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2829 928
Size: 971 930 3788
Fragments arranged by size:
3788 971 930
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2563 2597 2758 2792 2800 2834 3880
Size: 34 626 34 161 34 8 34 1046
Cuts at: 3880 3914 1903
Size: 34 3678
Fragments arranged by size:
3678 1046 626 161 34 34 34 34
34 8
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2073 4300 136
Size: 107 122 71 631 605 401 2227 1525
Fragments arranged by size:
2227 1525 631 605 401 122 107 71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 2081 3106 2081
Size: 1025 4664
Bme1580I G_kGCm'C
Cuts at: 1790 2418 2604 2749 3750 4906 1790
Size: 628 186 145 1001 1156 2573
Fragments arranged by size:
2573 1156 1001 628 186 145
BmgBI CAC'GTC
Cuts at: 1318 2062 1318
Size: 744 4945
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2712 3420 4378
Size: 416 53 83 399 24 1295 708 958
Cuts at: 4378 442
Size: 1753
Fragments arranged by size:
1753 1295 958 708 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2960 2960
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 4349 1239
Size: 1031 2079 2579
Fragments arranged by size:
2579 2079 1031
Bpu10I CC'TnA_GC
Cuts at: 2570 2570
Size: 5689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3814 4679 4977 3814
Size: 865 298 4526
Fragments arranged by size:
4526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2166 2965 4352 882
Size: 617 667 799 1387 2219
Fragments arranged by size:
2219 1387 799 667 617
BsaAI yAC'GTr
Cuts at: 493 1204 2881 3480 493
Size: 711 1677 599 2702
Fragments arranged by size:
2702 1677 711 599
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2167 3496 3878 4513
Size: 53 83 186 936 634 1329 382 635
Cuts at: 4513 275
Size: 1451
Fragments arranged by size:
1451 1329 936 635 634 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2352 2439 4122 4520 4794 4941 5352 2352
Size: 87 1683 398 274 147 411 2689
Fragments arranged by size:
2689 1683 411 398 274 147 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2347 2377 2891 2921 1178
Size: 30 165 30 944 30 514 30 3946
Fragments arranged by size:
3946 944 514 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2617 1186
Size: 1431 4258
BseYI C'CCAG_C
Cuts at: 2111 2417 2603 2647 2789 2921 4892 5388 2111
Size: 306 186 44 142 132 1971 496 2412
Fragments arranged by size:
2412 1971 496 306 186 142 132 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2448 1163
Size: 573 712 4404
Fragments arranged by size:
4404 712 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3750 3835 4906 3750
Size: 85 1071 4533
Fragments arranged by size:
4533 1071 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2240 2338 2418 2604 2749 3008 3750
Size: 399 450 98 80 186 145 259 742
Cuts at: 3750 3835 4906 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 450 399 259 186 145
98 85 80
BspEI T'CCGG_A
Cuts at: 2352 2439 5352 2352
Size: 87 2913 2689
Fragments arranged by size:
2913 2689 87
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3044 844
Size: 2200 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 2663 3576 1394
Size: 429 205 635 913 3507
Fragments arranged by size:
3507 913 635 429 205
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4333 4520 1700
Size: 110 2523 187 2869
Fragments arranged by size:
2869 2523 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2306 2062
Size: 244 5445
BstYI r'GATC_y
Cuts at: 1972 3019 3386 3771 3788 5240 5326 5338 5684
Size: 1047 367 385 17 1452 86 12 346
Cuts at: 5684 1972
Size: 1977
Fragments arranged by size:
1977 1452 1047 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2803 487
Size: 683 56 1577 3373
Fragments arranged by size:
3373 1577 683 56
BtsI GCAGTG_nn'
Cuts at: 2217 3205 3999 4019 2217
Size: 988 794 20 3887
Fragments arranged by size:
3887 988 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2206 2241 529
Size: 35 1642 35 3977
Fragments arranged by size:
3977 1642 35 35
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 3136 4025
Size: 54 1425 50 275 18 261 1045 889
Cuts at: 4025 5654 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1045 889 275 261 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2122 2245 2590 3619 1353
Size: 769 123 345 1029 3423
Fragments arranged by size:
3423 1029 769 345 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 3752 4349 5136 5404 1239
Size: 393 638 1482 597 787 268 1524
Fragments arranged by size:
1524 1482 787 638 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2381 2381
Size: 5689
EcoO109I rG'GnC_Cy
Cuts at: 2404 2414 2745 2404
Size: 10 331 5348
Fragments arranged by size:
5348 331 10
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 4836 1678
Size: 3158 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2163 2195 2196
Size: 32 135 32 455 32 104 32 1
Cuts at: 2196 2228 2266 2298 4330 4362 4404 4436 1373
Size: 32 38 32 2032 32 42 32 2626
Fragments arranged by size:
2626 2032 455 135 104 42 38 32
32 32 32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2347 3033 3279 669
Size: 988 281 409 686 246 3079
Fragments arranged by size:
3079 988 686 409 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 2398 3014 2398
Size: 616 5073
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2058 2070 4803 4987 1305
Size: 753 12 2733 184 2007
Fragments arranged by size:
2733 2007 753 184 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2093 10
Size: 54 1425 343 261 3606
Fragments arranged by size:
3606 1425 343 261 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 3647 4006 4165 518
Size: 648 894 1587 359 159 2042
Fragments arranged by size:
2042 1587 894 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2080 2101 2921 2933 3105 3782 4930 5175
Size: 474 21 820 12 172 677 1148 245
Cuts at: 5175 1606
Size: 2120
Fragments arranged by size:
2120 1148 820 677 474 245 172 21
12
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5137
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 3047 4592 1132
Size: 97 1818 1545 2229
Fragments arranged by size:
2229 1818 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2912 1348
Size: 1564 4125
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5689
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2404 2404
Size: 5689
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PspOMI G'GGCC_C
Cuts at: 2414 2745 2414
Size: 331 5358
PstI C_TGCA'G
Cuts at: 830 2835 3041 830
Size: 2005 206 3478
Fragments arranged by size:
3478 2005 206
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
PvuII CAG'CTG
Cuts at: 2080 2921 2080
Size: 841 4848
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SapI GCTCTTCn'nnn_
Cuts at: 2122 2245 2122
Size: 123 5566
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 2831 2862 3037 4172 4853 5044 826
Size: 219 1786 31 175 1135 681 191 1471
Fragments arranged by size:
1786 1471 1135 681 219 191 175 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 2073 2073
Size: 5689
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 3016 1872
Size: 1144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3793 4694 4956 820
Size: 197 926 1850 901 262 1553
Fragments arranged by size:
1850 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 2016 3128 513
Size: 552 951 1112 3074
Fragments arranged by size:
3074 1112 951 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3716 3733 3886 4071 4516 325
Size: 1255 2136 17 153 185 445 1498
Fragments arranged by size:
2136 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3935 5647 96
Size: 274 80 33 51 1443 1958 1712 138
Fragments arranged by size:
1958 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5649 5681 5649
Size: 32 5657
Tth111I GACn'n_nGTC
Cuts at: 1316 2435 1316
Size: 1119 4570
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2306 2509 2306
Size: 203 5486
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 3014 1870
Size: 1144 4545
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfoI SrfI SwaI