Restriction map for
pFN21AB7646
(Circular) (Six-base) MAPSORT of: pFN21AB7646.seq Check: 3434 from: 1 to: 5875
pFN21AB7646 5875 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3685 278
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3218 1937
Size: 1281 4594
Acc65I G'GTAC_C
Cuts at: 3196 3196
Size: 5875
AclI AA'CG_TT
Cuts at: 1563 4002 4375 1563
Size: 2439 373 3063
Fragments arranged by size:
3063 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3104 3938 5322 5590 1632
Size: 1472 834 1384 268 1917
Fragments arranged by size:
1917 1472 1384 834 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5678
AflIII A'CryG_T
Cuts at: 1203 4774 5751 1203
Size: 3571 977 1327
Fragments arranged by size:
3571 1327 977
AgeI A'CCGG_T
Cuts at: 4706 4706
Size: 5875
AhdI GACnn_n'nnGTC
Cuts at: 3156 4604 3156
Size: 1448 4427
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2331 1184
Size: 1147 4728
AlwNI CAG_nnn'CTG
Cuts at: 2812 2989 3618 5190 2812
Size: 177 629 1572 3497
Fragments arranged by size:
3497 1572 629 177
ApaLI G'TGCA_C
Cuts at: 3932 5088 3932
Size: 1156 4719
ApoI r'AATT_y
Cuts at: 1493 3184 3410 3562 5671 1493
Size: 1691 226 152 2109 1697
Fragments arranged by size:
2109 1697 1691 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 3200 1870
Size: 73 1257 4545
Fragments arranged by size:
4545 1257 73
BamHI G'GATC_C
Cuts at: 3205 3572 3205
Size: 367 5508
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3196 4651 618
Size: 325 217 627 1409 1455 1842
Fragments arranged by size:
1842 1455 1409 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2763 3194 1391
Size: 1372 431 4072
Fragments arranged by size:
4072 1372 431
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2419 2793 3027 3070 3089 928
Size: 971 520 374 234 43 19 3714
Fragments arranged by size:
3714 971 520 374 234 43 19
BbvCI CC'TCA_GC
Cuts at: 3122 3122
Size: 5875
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4066 4100 1903
Size: 34 2129 34 3678
Fragments arranged by size:
3678 2129 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2528 3767 4977 2528
Size: 1239 1210 3426
Fragments arranged by size:
3426 1239 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4486 136
Size: 107 122 71 631 605 2814 1525
Fragments arranged by size:
2814 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2104 5870 2104
Size: 3766 2109
BlpI GC'TnA_GC
Cuts at: 3292 3292
Size: 5875
Bme1580I G_kGCm'C
Cuts at: 1790 2752 3130 3936 5092 1790
Size: 962 378 806 1156 2573
Fragments arranged by size:
2573 1156 962 806 378
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5875
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2544 2643 3606
Size: 416 53 83 399 24 1127 99 963
Cuts at: 3606 4564 442
Size: 958 1753
Fragments arranged by size:
1753 1127 963 958 416 399 99 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5875
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2708 2708
Size: 5875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2406 4535 1239
Size: 1167 2129 2579
Fragments arranged by size:
2579 2129 1167
Bpu10I CC'TnA_GC
Cuts at: 3122 3122
Size: 5875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2012 3070 4000 4865 5163 2012
Size: 1058 930 865 298 2724
Fragments arranged by size:
2724 1058 930 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2052 3164 4538 882
Size: 617 553 1112 1374 2219
Fragments arranged by size:
2219 1374 1112 617 553
BsaAI yAC'GTr
Cuts at: 493 1204 2072 3666 493
Size: 711 868 1594 2702
Fragments arranged by size:
2702 1594 868 711
BsaBI GATnn'nnATC
Cuts at: 5869 5869
Size: 5875
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3682 4064 4699 275
Size: 53 83 186 936 2149 382 635 1451
Fragments arranged by size:
2149 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2531 4308 4706 4980 5127 5538 2531
Size: 1777 398 274 147 411 2868
Fragments arranged by size:
2868 1777 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5650
Fragments arranged by size:
5650 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5875
BseYI C'CCAG_C
Cuts at: 5078 5574 5078
Size: 496 5379
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5302
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3325 4086 5114 1540
Size: 275 186 1324 761 1028 2301
Fragments arranged by size:
2301 1324 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2763 3936 4021 5092 2763
Size: 1173 85 1071 3546
Fragments arranged by size:
3546 1173 1071 85
BsmI GAATG_Cn'
Cuts at: 3386 3479 3386
Size: 93 5782
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2752 2763 3130 3194 3936 4021 5092
Size: 399 962 11 367 64 742 85 1071
Cuts at: 5092 1391
Size: 2174
Fragments arranged by size:
2174 1071 962 742 399 367 85 64
11
BspEI T'CCGG_A
Cuts at: 2531 5538 2531
Size: 3007 2868
BspHI T'CATG_A
Cuts at: 2394 3764 5494 2394
Size: 1370 1730 2775
Fragments arranged by size:
2775 1730 1370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3230 844
Size: 2386 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3762 1394
Size: 429 1939 3507
Fragments arranged by size:
3507 1939 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4370 4544 65
Size: 1183 3122 174 1396
Fragments arranged by size:
3122 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3155 4519 4706 1700
Size: 110 1345 1364 187 2869
Fragments arranged by size:
2869 1364 1345 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5875
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5875
BssSI C'ACGA_G
Cuts at: 3935 4947 3935
Size: 1012 4863
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5875
BstBI TT'CG_AA
Cuts at: 4679 4679
Size: 5875
BstEII G'GTnAC_C
Cuts at: 1184 2555 2801 2824 1184
Size: 1371 246 23 4235
Fragments arranged by size:
4235 1371 246 23
BstYI r'GATC_y
Cuts at: 1972 2104 2985 3205 3572 3957 3974 5426 5512
Size: 132 881 220 367 385 17 1452 86
Cuts at: 5512 5524 5870 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 881 385 367 346 220 132
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4384
Fragments arranged by size:
4384 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5136
Fragments arranged by size:
5136 683 56
BtsI GCAGTG_nn'
Cuts at: 3391 4185 4205 3391
Size: 794 20 5061
Fragments arranged by size:
5061 794 20
ClaI AT'CG_AT
Cuts at: 1554 2946 1554
Size: 1392 4483
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2533 2568 529
Size: 35 1969 35 3836
Fragments arranged by size:
3836 1969 35 35
DraI TTT'AAA
Cuts at: 3179 3179
Size: 5875
DraIII CAC_nnn'GTG
Cuts at: 2582 2582
Size: 5875
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3074 4882 809
Size: 507 1758 1808 1802
Fragments arranged by size:
1808 1802 1758 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3322 4211 5840
Size: 54 1425 50 275 18 1492 889 1629
Cuts at: 5840 8
Size: 43
Fragments arranged by size:
1629 1492 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3322 1537
Size: 275 1510 4090
Fragments arranged by size:
4090 1510 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2811 3805 1353
Size: 1458 994 3423
Fragments arranged by size:
3423 1458 994
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2445 2740 4462 4836 4982 387
Size: 1064 138 856 295 1722 374 146 1280
Fragments arranged by size:
1722 1280 1064 856 374 295 146 138
EcoICRI GAG'CTC
Cuts at: 2761 2761
Size: 5875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2406 3104 3938 4535 5322 5590 1239
Size: 393 774 698 834 597 787 268 1524
Fragments arranged by size:
1524 834 787 774 698 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2193 2795 3052 2193
Size: 602 257 5016
Fragments arranged by size:
5016 602 257
EcoRI G'AATT_C
Cuts at: 3184 3562 3184
Size: 378 5497
EcoRV GAT'ATC
Cuts at: 2944 2944
Size: 5875
FspI TGC'GCA
Cuts at: 4381 4381
Size: 5875
HaeII r_GCGC'y
Cuts at: 1678 5022 1678
Size: 3344 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2977 3009 3142 3174 4516
Size: 32 135 32 1405 32 133 32 1342
Cuts at: 4516 4548 4590 4622 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1405 1342 135 133 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 3465 669
Size: 988 281 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5875
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KpnI G_GTAC'C
Cuts at: 3200 3200
Size: 5875
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1995 4989 5173 1305
Size: 690 2994 184 2007
Fragments arranged by size:
2994 2007 690 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4053
Fragments arranged by size:
4053 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2313 2399 3833 4192 4351 518
Size: 648 843 304 86 1434 359 159 2042
Fragments arranged by size:
2042 1434 843 648 359 304 159 86
MspA1I CmG'CkG
Cuts at: 1606 3121 3291 3968 5116 5361 1606
Size: 1515 170 677 1148 245 2120
Fragments arranged by size:
2120 1515 1148 677 245 170
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4384
Fragments arranged by size:
4384 939 552
NdeI CA'TA_TG
Cuts at: 387 2128 387
Size: 1741 4134
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5875
NotI GC'GGCC_GC
Cuts at: 3322 3322
Size: 5875
NspI r_CATG'y
Cuts at: 1132 1229 2312 2497 2506 3092 3233 4778 1132
Size: 97 1083 185 9 586 141 1545 2229
Fragments arranged by size:
2229 1545 1083 586 185 141 97 9
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5875
PciI A'CATG_T
Cuts at: 4774 4774
Size: 5875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2582 2812 1108
Size: 1474 230 4171
Fragments arranged by size:
4171 1474 230
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 5457 5489 3944
Size: 32 1481 32 4330
Fragments arranged by size:
4330 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5875
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PstI C_TGCA'G
Cuts at: 830 3227 830
Size: 2397 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
PvuII CAG'CTG
Cuts at: 3121 3121
Size: 5875
SacI G_AGCT'C
Cuts at: 2763 2763
Size: 5875
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SbfI CC_TGCA'GG
Cuts at: 3227 3227
Size: 5875
ScaI AGT'ACT
Cuts at: 4123 5835 4123
Size: 1712 4163
SexAI A'CCwGG_T
Cuts at: 2578 2578
Size: 5875
SfcI C'TryA_G
Cuts at: 826 1045 3223 4358 5039 5230 826
Size: 219 2178 1135 681 191 1471
Fragments arranged by size:
2178 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 3202 1872
Size: 1330 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2027 3049 3979 4880 5142 820
Size: 197 926 84 1022 930 901 262 1553
Fragments arranged by size:
1553 1022 930 926 901 262 197 84
SnaBI TAC'GTA
Cuts at: 493 3666 493
Size: 3173 2702
SpeI A'CTAG_T
Cuts at: 152 2279 152
Size: 2127 3748
SphI G_CATG'C
Cuts at: 1132 2506 3233 1132
Size: 1374 727 3774
Fragments arranged by size:
3774 1374 727
SspI AAT'ATT
Cuts at: 5 52 2212 3799 5
Size: 47 2160 1587 2081
Fragments arranged by size:
2160 2081 1587 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5875
StyI C'CwwG_G
Cuts at: 513 1065 2004 3314 513
Size: 552 939 1310 3074
Fragments arranged by size:
3074 1310 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2376 3902 3919 4072 4257 4702 325
Size: 1255 796 1526 17 153 185 445 1498
Fragments arranged by size:
1526 1498 1255 796 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2046 4121 5833
Size: 274 80 33 51 1443 69 2075 1712
Cuts at: 5833 96
Size: 138
Fragments arranged by size:
2075 1712 1443 274 138 80 69 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2629 2661 5835 5867 2629
Size: 32 3174 32 2637
Fragments arranged by size:
3174 2637 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5875
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2223 2223
Size: 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5875
XmaI C'CCGG_G
Cuts at: 1870 3200 1870
Size: 1330 4545
XmnI GAAnn'nnTTC
Cuts at: 4004 4004
Size: 5875
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3683 276
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoO109I FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI