Restriction map for
pFN21AB7642
(Circular) (Six-base) MAPSORT of: pFN21AB7642.seq Check: 1563 from: 1 to: 5866
pFN21AB7642 5866 bp
With 182 enzymes: SgfI *
March 28, 2012 13:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3676 278
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3209 1937
Size: 1272 4594
Acc65I G'GTAC_C
Cuts at: 2841 3187 2841
Size: 346 5520
AclI AA'CG_TT
Cuts at: 1563 3993 4366 1563
Size: 2430 373 3063
Fragments arranged by size:
3063 2430 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2533 3929 5313 5581 1632
Size: 901 1396 1384 268 1917
Fragments arranged by size:
1917 1396 1384 901 268
AfeI AGC'GCT
Cuts at: 2808 2808
Size: 5866
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5669
AflIII A'CryG_T
Cuts at: 1203 4765 5742 1203
Size: 3562 977 1327
Fragments arranged by size:
3562 1327 977
AgeI A'CCGG_T
Cuts at: 4697 4697
Size: 5866
AhdI GACnn_n'nnGTC
Cuts at: 4595 4595
Size: 5866
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5866
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2552 1184
Size: 1368 4498
AlwNI CAG_nnn'CTG
Cuts at: 2347 2386 3609 5181 2347
Size: 39 1223 1572 3032
Fragments arranged by size:
3032 1572 1223 39
ApaLI G'TGCA_C
Cuts at: 2475 2537 3923 5079 2475
Size: 62 1386 1156 3262
Fragments arranged by size:
3262 1386 1156 62
ApoI r'AATT_y
Cuts at: 1493 3175 3401 3553 5662 1493
Size: 1682 226 152 2109 1697
Fragments arranged by size:
2109 1697 1682 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4420 160
Size: 872 3388 1606
Fragments arranged by size:
3388 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
AvaI C'yCGr_G
Cuts at: 1870 1943 2561 3162 3191 1870
Size: 73 618 601 29 4545
Fragments arranged by size:
4545 618 601 73 29
AvrII C'CTAG_G
Cuts at: 3097 3097
Size: 5866
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2478 2511 2478
Size: 33 5833
BamHI G'GATC_C
Cuts at: 3196 3563 3196
Size: 367 5499
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2185 2841 3073 3149 3187
Size: 325 217 627 398 656 232 76 38
Cuts at: 3187 4642 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 656 627 398 325 232 217
76 38
BanII G_rGCy'C
Cuts at: 1391 2220 2393 3185 1391
Size: 829 173 792 4072
Fragments arranged by size:
4072 829 792 173
BbeI G_GCGC'C
Cuts at: 2189 2189
Size: 5866
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2415 928
Size: 971 516 4379
Fragments arranged by size:
4379 971 516
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4057 4091 1903
Size: 34 2120 34 3678
Fragments arranged by size:
3678 2120 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2506 3758 4968 2506
Size: 1252 1210 3404
Fragments arranged by size:
3404 1252 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5866
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2402 2970 4477
Size: 107 122 71 631 605 730 568 1507
Cuts at: 4477 136
Size: 1525
Fragments arranged by size:
1525 1507 730 631 605 568 122 107
71
BglII A'GATC_T
Cuts at: 5861 5861
Size: 5866
BlpI GC'TnA_GC
Cuts at: 3283 3283
Size: 5866
Bme1580I G_kGCm'C
Cuts at: 1790 2479 2541 3078 3927 5083 1790
Size: 689 62 537 849 1156 2573
Fragments arranged by size:
2573 1156 849 689 537 62
BmgBI CAC'GTC
Cuts at: 1318 2421 1318
Size: 1103 4763
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3597 4555 442
Size: 416 53 83 399 24 2180 958 1753
Fragments arranged by size:
2180 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2765 2909 3024 4526 1239
Size: 1526 144 115 1502 2579
Fragments arranged by size:
2579 1526 1502 144 115
Bpu10I CC'TnA_GC
Cuts at: 2774 2774
Size: 5866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3991 4856 5154 3991
Size: 865 298 4703
Fragments arranged by size:
4703 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2640 4529 882
Size: 617 1141 1889 2219
Fragments arranged by size:
2219 1889 1141 617
BsaAI yAC'GTr
Cuts at: 493 1204 3657 493
Size: 711 2453 2702
Fragments arranged by size:
2702 2453 711
BsaBI GATnn'nnATC
Cuts at: 5860 5860
Size: 5866
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2186 3673 4055 4690
Size: 53 83 186 936 653 1487 382 635
Cuts at: 4690 275
Size: 1451
Fragments arranged by size:
1487 1451 936 653 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3030 4299 4697 4971 5118 5529 3030
Size: 1269 398 274 147 411 3367
Fragments arranged by size:
3367 1269 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2137 2167 1178
Size: 30 165 30 734 30 4877
Fragments arranged by size:
4877 734 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3027 1186
Size: 1841 4025
BseYI C'CCAG_C
Cuts at: 2250 2379 2386 2968 3054 5069 5565 2250
Size: 129 7 582 86 2015 496 2551
Fragments arranged by size:
2551 2015 582 496 129 86 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2801 1163
Size: 573 1065 4228
Fragments arranged by size:
4228 1065 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2298 2397 3316 4077 5105 1540
Size: 275 186 297 99 919 761 1028 2301
Fragments arranged by size:
2301 1028 919 761 297 275 186 99
BsiHKAI G_wGCw'C
Cuts at: 2479 2541 3087 3927 4012 5083 2479
Size: 62 546 840 85 1071 3262
Fragments arranged by size:
3262 1071 840 546 85 62
BsmI GAATG_Cn'
Cuts at: 3377 3470 3377
Size: 93 5773
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 2393 2479 2541 3078 3087 3185
Size: 399 430 173 86 62 537 9 98
Cuts at: 3185 3927 4012 5083 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 537 430 399 173 98
86 85 62 9
BspEI T'CCGG_A
Cuts at: 5529 5529
Size: 5866
BspHI T'CATG_A
Cuts at: 3755 5485 3755
Size: 1730 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3221 844
Size: 2377 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2107 2761 3753 1394
Size: 429 284 654 992 3507
Fragments arranged by size:
3507 992 654 429 284
BsrDI GCAATG_nn'
Cuts at: 65 1248 4361 4535 65
Size: 1183 3113 174 1396
Fragments arranged by size:
3113 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2293 4510 4697 1700
Size: 110 483 2217 187 2869
Fragments arranged by size:
2869 2217 483 187 110
BsrGI T'GTAC_A
Cuts at: 96 3015 96
Size: 2919 2947
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5866
BssSI C'ACGA_G
Cuts at: 2540 3926 4938 2540
Size: 1386 1012 3468
Fragments arranged by size:
3468 1386 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2317 2326 2347 794
Size: 1523 9 21 4313
Fragments arranged by size:
4313 1523 21 9
BstBI TT'CG_AA
Cuts at: 4670 4670
Size: 5866
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5866
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5866
BstYI r'GATC_y
Cuts at: 1972 3196 3563 3948 3965 5417 5503 5515 5861
Size: 1224 367 385 17 1452 86 12 346
Cuts at: 5861 1972
Size: 1977
Fragments arranged by size:
1977 1452 1224 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2124 2211 2298 2626 3091 513
Size: 552 1059 87 87 328 465 3288
Fragments arranged by size:
3288 1059 552 465 328 87 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2925 487
Size: 683 56 1699 3428
Fragments arranged by size:
3428 1699 683 56
BtsI GCAGTG_nn'
Cuts at: 2541 3382 4176 4196 2541
Size: 841 794 20 4211
Fragments arranged by size:
4211 841 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5831
DraI TTT'AAA
Cuts at: 3170 3170
Size: 5866
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2419 2828 4873 809
Size: 507 1103 409 2045 1802
Fragments arranged by size:
2045 1802 1103 507 409
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 2394 2898
Size: 54 1425 50 275 18 465 99 504
Cuts at: 2898 3313 4202 5831 8
Size: 415 889 1629 43
Fragments arranged by size:
1629 1425 889 504 465 415 275 99
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2295 2394 3313 1537
Size: 275 483 99 919 4090
Fragments arranged by size:
4090 919 483 275 99
EarI CTCTTCn'nnn_
Cuts at: 1353 3796 1353
Size: 2443 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2090 4453 4827 4973 387
Size: 1064 138 501 2363 374 146 1280
Fragments arranged by size:
2363 1280 1064 501 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2533 2765 2909 3024 3929 4526 5313
Size: 393 901 232 144 115 905 597 787
Cuts at: 5313 5581 1239
Size: 268 1524
Fragments arranged by size:
1524 905 901 787 597 393 268 232
144 115
EcoO109I rG'GnC_Cy
Cuts at: 3101 3101
Size: 5866
EcoRI G'AATT_C
Cuts at: 3175 3553 3175
Size: 378 5488
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2461 2733 2461
Size: 272 5594
FspI TGC'GCA
Cuts at: 4372 4372
Size: 5866
HaeII r_GCGC'y
Cuts at: 1678 2189 2810 2924 5013 1678
Size: 511 621 114 2089 2531
Fragments arranged by size:
2531 2089 621 511 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2239 2271 4507 4539 4581
Size: 32 135 32 667 32 2236 32 42
Cuts at: 4581 4613 1373
Size: 32 2626
Fragments arranged by size:
2626 2236 667 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3210 3456 669
Size: 988 281 1272 246 3079
Fragments arranged by size:
3079 1272 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5866
HpaI GTT'AAC
Cuts at: 3456 3456
Size: 5866
KasI G'GCGC_C
Cuts at: 2185 2185
Size: 5866
KpnI G_GTAC'C
Cuts at: 2845 3191 2845
Size: 346 5520
MfeI C'AATT_G
Cuts at: 3465 3465
Size: 5866
MluI A'CGCG_T
Cuts at: 5742 5742
Size: 5866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2748 4980 5164 1305
Size: 1443 2232 184 2007
Fragments arranged by size:
2232 2007 1443 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4044
Fragments arranged by size:
4044 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3824 4183 4342 518
Size: 648 843 1815 359 159 2042
Fragments arranged by size:
2042 1815 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2025 2126 2300 2347 2386 2879 3008 3058
Size: 419 101 174 47 39 493 129 50
Cuts at: 3058 3064 3282 3959 5107 5352 1606
Size: 6 218 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 493 419 245 218 174
129 101 50 47 39 6
NaeI GCC'GGC
Cuts at: 2295 2295
Size: 5866
NarI GG'CG_CC
Cuts at: 2186 2186
Size: 5866
NcoI C'CATG_G
Cuts at: 513 1065 2211 513
Size: 552 1146 4168
Fragments arranged by size:
4168 1146 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5866
NgoMIV G'CCGG_C
Cuts at: 2293 2293
Size: 5866
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5866
NotI GC'GGCC_GC
Cuts at: 3313 3313
Size: 5866
NspI r_CATG'y
Cuts at: 1132 1229 2475 3028 3224 4769 1132
Size: 97 1246 553 196 1545 2229
Fragments arranged by size:
2229 1545 1246 553 196 97
PacI TTA_AT'TAA
Cuts at: 2436 2436
Size: 5866
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5866
PciI A'CATG_T
Cuts at: 4765 4765
Size: 5866
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2386 1108
Size: 1278 4588
PmeI GTTT'AAAC
Cuts at: 3170 3170
Size: 5866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 5448 5480 3935
Size: 32 1481 32 4321
Fragments arranged by size:
4321 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5866
PsiI TTA'TAA
Cuts at: 3436 3436
Size: 5866
PspXI vC'TCGA_Gb
Cuts at: 2561 2561
Size: 5866
PstI C_TGCA'G
Cuts at: 830 2806 2928 3063 3218 830
Size: 1976 122 135 155 3478
Fragments arranged by size:
3478 1976 155 135 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5866
PvuII CAG'CTG
Cuts at: 2386 3058 2386
Size: 672 5194
SacII CC_GC'GG
Cuts at: 2127 2301 2127
Size: 174 5692
SalI G'TCGA_C
Cuts at: 1936 3208 1936
Size: 1272 4594
SbfI CC_TGCA'GG
Cuts at: 3218 3218
Size: 5866
ScaI AGT'ACT
Cuts at: 4114 5826 4114
Size: 1712 4154
SexAI A'CCwGG_T
Cuts at: 2837 2837
Size: 5866
SfcI C'TryA_G
Cuts at: 826 1045 2482 2802 2924 3059 3214 4349 5030
Size: 219 1437 320 122 135 155 1135 681
Cuts at: 5030 5221 826
Size: 191 1471
Fragments arranged by size:
1471 1437 1135 681 320 219 191 155
135 122
SfiI GGCCn_nnn'nGGCC
Cuts at: 2402 2402
Size: 5866
SfoI GGC'GCC
Cuts at: 2187 2187
Size: 5866
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5866
SmaI CCC'GGG
Cuts at: 1872 3164 3193 1872
Size: 1292 29 4545
Fragments arranged by size:
4545 1292 29
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2561 3970 4871 5133 820
Size: 197 926 618 1409 901 262 1553
Fragments arranged by size:
1553 1409 926 901 618 262 197
SnaBI TAC'GTA
Cuts at: 493 3657 493
Size: 3164 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5866
SphI G_CATG'C
Cuts at: 1132 3224 1132
Size: 2092 3774
SspI AAT'ATT
Cuts at: 5 52 3790 5
Size: 47 3738 2081
Fragments arranged by size:
3738 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5866
StyI C'CwwG_G
Cuts at: 513 1065 2211 3097 3305 513
Size: 552 1146 886 208 3074
Fragments arranged by size:
3074 1146 886 552 208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2840 2973 3893 3910 4063 4248 4693
Size: 1255 1260 133 920 17 153 185 445
Cuts at: 4693 325
Size: 1498
Fragments arranged by size:
1498 1260 1255 920 445 185 153 133
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3015 4112 5824
Size: 274 80 33 51 1443 1038 1097 1712
Cuts at: 5824 96
Size: 138
Fragments arranged by size:
1712 1443 1097 1038 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2137 2169 5826 5858 2137
Size: 32 3657 32 2145
Fragments arranged by size:
3657 2145 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5866
XbaI T'CTAG_A
Cuts at: 3202 3202
Size: 5866
XhoI C'TCGA_G
Cuts at: 1943 2561 1943
Size: 618 5248
XmaI C'CCGG_G
Cuts at: 1870 3162 3191 1870
Size: 1292 29 4545
Fragments arranged by size:
4545 1292 29
XmnI GAAnn'nnTTC
Cuts at: 3995 3995
Size: 5866
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3674 276
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PacI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI
PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI NruI NsiI PfoI PmlI PpuMI PspOMI PsrI
RsrII SacI SanDI SapI SrfI SwaI XcmI