Restriction map for
pFN21AB7641
(Circular) (Six-base) MAPSORT of: pFN21AB7641.seq Check: 2350 from: 1 to: 5683
pFN21AB7641 5683 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2589 2673 2589
Size: 84 5599
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3026 1937
Size: 1089 4594
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3746 5130 5398 1632
Size: 2114 1384 268 1917
Fragments arranged by size:
2114 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 2751 4582 5559 1203
Size: 1548 1831 977 1327
Fragments arranged by size:
1831 1548 1327 977
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 4412 4412
Size: 5683
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5683
AlwNI CAG_nnn'CTG
Cuts at: 2209 3426 4998 2209
Size: 1217 1572 2894
Fragments arranged by size:
2894 1572 1217
ApaI G_GGCC'C
Cuts at: 2105 2366 2715 2945 2105
Size: 261 349 230 4843
Fragments arranged by size:
4843 349 261 230
ApaLI G'TGCA_C
Cuts at: 2162 3740 4896 2162
Size: 1578 1156 2949
Fragments arranged by size:
2949 1578 1156
ApoI r'AATT_y
Cuts at: 1493 2992 3218 3370 5479 1493
Size: 1499 226 152 2109 1697
Fragments arranged by size:
2109 1697 1499 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 2156 2527 2546 2605 2708 2818 3008
Size: 73 213 371 19 59 103 110 190
Cuts at: 3008 1870
Size: 4545
Fragments arranged by size:
4545 371 213 190 110 103 73 59
19
BamHI G'GATC_C
Cuts at: 3013 3380 3013
Size: 367 5316
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2064 2341 2411 2437
Size: 325 217 627 230 47 277 70 26
Cuts at: 2437 2497 2531 2670 2865 3004 4459 618
Size: 60 34 139 195 139 1455 1842
Fragments arranged by size:
1842 1455 627 325 277 230 217 195
139 139 70 60 47 34 26
BanII G_rGCy'C
Cuts at: 1391 2105 2298 2366 2481 2715 2945 3002 1391
Size: 714 193 68 115 234 230 57 4072
Fragments arranged by size:
4072 714 234 230 193 115 68 57
BbeI G_GCGC'C
Cuts at: 2441 2501 2674 2869 2441
Size: 60 173 195 5255
Fragments arranged by size:
5255 195 173 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4712
BbvCI CC'TCA_GC
Cuts at: 2427 2427
Size: 5683
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2842 2876 3874 3908 1903
Size: 34 905 34 998 34 3678
Fragments arranged by size:
3678 998 905 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5683
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2423 2607 4294
Size: 107 122 71 631 605 751 184 1687
Cuts at: 4294 136
Size: 1525
Fragments arranged by size:
1687 1525 751 631 605 184 122 107
71
BglII A'GATC_T
Cuts at: 5678 5678
Size: 5683
BlpI GC'TnA_GC
Cuts at: 3100 3100
Size: 5683
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2105 2166 2366 2534 2715 2945 3744
Size: 232 83 61 200 168 181 230 799
Cuts at: 3744 4900 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 799 232 230 200 181 168
83 61
BmgBI CAC'GTC
Cuts at: 1318 2538 1318
Size: 1220 4463
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3414 4372 442
Size: 416 53 83 399 24 1997 958 1753
Fragments arranged by size:
1997 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2361 2361
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2309 4343 1239
Size: 1070 2034 2579
Fragments arranged by size:
2579 2034 1070
Bpu10I CC'TnA_GC
Cuts at: 2210 2427 2210
Size: 217 5466
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3808 4673 4971 3808
Size: 865 298 4520
Fragments arranged by size:
4520 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4346 882
Size: 617 2847 2219
Fragments arranged by size:
2847 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2878 3474 493
Size: 711 1674 596 2702
Fragments arranged by size:
2702 1674 711 596
BsaBI GATnn'nnATC
Cuts at: 5677 5677
Size: 5683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2438 2498 2671 2866
Size: 53 83 186 936 905 60 173 195
Cuts at: 2866 3490 3872 4507 275
Size: 624 382 635 1451
Fragments arranged by size:
1451 936 905 635 624 382 195 186
173 83 60 53
BsaWI w'CCGG_w
Cuts at: 4116 4514 4788 4935 5346 4116
Size: 398 274 147 411 4453
Fragments arranged by size:
4453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2165 2195 1178
Size: 30 165 30 762 30 4666
Fragments arranged by size:
4666 762 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2306 1186
Size: 1099 21 4563
Fragments arranged by size:
4563 1099 21
BseYI C'CCAG_C
Cuts at: 2116 2480 2841 4886 5382 2116
Size: 364 361 2045 496 2417
Fragments arranged by size:
2417 2045 496 364 361
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2266 1163
Size: 573 530 4580
Fragments arranged by size:
4580 573 530
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2905 3133 3894 4922 1540
Size: 275 186 904 228 761 1028 2301
Fragments arranged by size:
2301 1028 904 761 275 228 186
BsiHKAI G_wGCw'C
Cuts at: 2166 2298 2664 2793 2877 3744 3829 4900 2166
Size: 132 366 129 84 867 85 1071 2949
Fragments arranged by size:
2949 1071 867 366 132 129 85 84
BsmI GAATG_Cn'
Cuts at: 3194 3287 3194
Size: 93 5590
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2105 2166 2298 2366 2481 2534
Size: 399 232 83 61 132 68 115 53
Cuts at: 2534 2664 2715 2793 2877 2945 3002 3744 3829
Size: 130 51 78 84 68 57 742 85
Cuts at: 3829 4900 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 232 132 130 115
85 84 83 78 68 68 61 57
53 51
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 3572 5302 3572
Size: 1730 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2589 2673 3038 844
Size: 1745 84 365 3489
Fragments arranged by size:
3489 1745 365 84
BsrBI CCG'CTC
Cuts at: 1394 1823 2265 3570 1394
Size: 429 442 1305 3507
Fragments arranged by size:
3507 1305 442 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4178 4352 65
Size: 1183 2930 174 1396
Fragments arranged by size:
2930 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2250 2434 2592 4327 4514 1700
Size: 110 440 184 158 1735 187 2869
Fragments arranged by size:
2869 1735 440 187 184 158 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5683
BssHII G'CGCG_C
Cuts at: 1925 2214 2919 1925
Size: 289 705 4689
Fragments arranged by size:
4689 705 289
BssSI C'ACGA_G
Cuts at: 3743 4755 3743
Size: 1012 4671
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2797 2881 794
Size: 2003 84 3596
Fragments arranged by size:
3596 2003 84
BstBI TT'CG_AA
Cuts at: 4487 4487
Size: 5683
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5683
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5683
BstYI r'GATC_y
Cuts at: 1972 3013 3380 3765 3782 5234 5320 5332 5678
Size: 1041 367 385 17 1452 86 12 346
Cuts at: 5678 1972
Size: 1977
Fragments arranged by size:
1977 1452 1041 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2346 2407 2346
Size: 61 5622
BtgI C'CryG_G
Cuts at: 513 1065 2004 2060 2105 2899 513
Size: 552 939 56 45 794 3297
Fragments arranged by size:
3297 939 794 552 56 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4944
Fragments arranged by size:
4944 683 56
BtsI GCAGTG_nn'
Cuts at: 3199 3993 4013 3199
Size: 794 20 4869
Fragments arranged by size:
4869 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DraIII CAC_nnn'GTG
Cuts at: 2797 2797
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2902 2913 3130
Size: 54 1425 50 275 18 1072 11 217
Cuts at: 3130 4019 5648 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1072 889 275 217 54 50
43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2902 3130 1537
Size: 275 1090 228 4090
Fragments arranged by size:
4090 1090 275 228
EarI CTCTTCn'nnn_
Cuts at: 1353 3613 1353
Size: 2260 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2063 4270 4644 4790 387
Size: 1064 138 474 2207 374 146 1280
Fragments arranged by size:
2207 1280 1064 474 374 146 138
EcoICRI GAG'CTC
Cuts at: 2296 2296
Size: 5683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2309 3746 4343 5130 5398 1239
Size: 393 677 1437 597 787 268 1524
Fragments arranged by size:
1524 1437 787 677 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2288 2288
Size: 5683
EcoO109I rG'GnC_Cy
Cuts at: 2041 2362 2363 2957 2041
Size: 321 1 594 4767
Fragments arranged by size:
4767 594 321 1
EcoRI G'AATT_C
Cuts at: 2992 3370 2992
Size: 378 5305
FspI TGC'GCA
Cuts at: 4189 4189
Size: 5683
HaeII r_GCGC'y
Cuts at: 1678 2051 2117 2441 2501 2674 2869 4830 1678
Size: 373 66 324 60 173 195 1961 2531
Fragments arranged by size:
2531 1961 373 324 195 173 66 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2312 2344 4324 4356 4398
Size: 32 135 32 740 32 1980 32 42
Cuts at: 4398 4430 1373
Size: 32 2626
Fragments arranged by size:
2626 1980 740 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3027 3273 669
Size: 988 281 1089 246 3079
Fragments arranged by size:
3079 1089 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KasI G'GCGC_C
Cuts at: 2437 2497 2670 2865 2437
Size: 60 173 195 5255
Fragments arranged by size:
5255 195 173 60
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 3282 3282
Size: 5683
MluI A'CGCG_T
Cuts at: 2751 5559 2751
Size: 2808 2875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 4797 4981 1305
Size: 858 2634 184 2007
Fragments arranged by size:
2634 2007 858 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3861
Fragments arranged by size:
3861 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3641 4000 4159 518
Size: 648 843 1632 359 159 2042
Fragments arranged by size:
2042 1632 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 2494 2912 3099 3776 4924 5169 1606
Size: 501 387 418 187 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 501 418 387 245 187
NaeI GCC'GGC
Cuts at: 2252 2436 2252
Size: 184 5499
NarI GG'CG_CC
Cuts at: 2438 2498 2671 2866 2438
Size: 60 173 195 5255
Fragments arranged by size:
5255 195 173 60
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4192
Fragments arranged by size:
4192 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5683
NgoMIV G'CCGG_C
Cuts at: 2250 2434 2250
Size: 184 5499
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 3041 4586 1132
Size: 97 1812 1545 2229
Fragments arranged by size:
2229 1812 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2366 2420 1348
Size: 1018 54 4611
Fragments arranged by size:
4611 1018 54
PciI A'CATG_T
Cuts at: 4582 4582
Size: 5683
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5683
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PmlI CAC'GTG
Cuts at: 2878 2878
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 5265 5297 3752
Size: 32 1481 32 4138
Fragments arranged by size:
4138 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5683
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PspOMI G'GGCC_C
Cuts at: 2101 2362 2711 2941 2101
Size: 261 349 230 4843
Fragments arranged by size:
4843 349 261 230
PstI C_TGCA'G
Cuts at: 830 3035 830
Size: 2205 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
SacI G_AGCT'C
Cuts at: 2298 2298
Size: 5683
SacII CC_GC'GG
Cuts at: 2108 2108
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 3931 5643 3931
Size: 1712 3971
SexAI A'CCwGG_T
Cuts at: 2793 2793
Size: 5683
SfcI C'TryA_G
Cuts at: 826 1045 3031 4166 4847 5038 826
Size: 219 1986 1135 681 191 1471
Fragments arranged by size:
1986 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2439 2499 2672 2867 2439
Size: 60 173 195 5255
Fragments arranged by size:
5255 195 173 60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 2529 2710 3010 1872
Size: 657 181 300 4545
Fragments arranged by size:
4545 657 300 181
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3787 4688 4950 820
Size: 197 926 1844 901 262 1553
Fragments arranged by size:
1844 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 2237 2645 1517
Size: 720 408 4555
Fragments arranged by size:
4555 720 408
StyI C'CwwG_G
Cuts at: 513 1065 2004 2169 2976 3122 513
Size: 552 939 165 807 146 3074
Fragments arranged by size:
3074 939 807 552 165 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2230 2969 3710 3727 3880 4065 4510
Size: 1255 650 739 741 17 153 185 445
Cuts at: 4510 325
Size: 1498
Fragments arranged by size:
1498 1255 741 739 650 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3929 5641 96
Size: 274 80 33 51 1443 1952 1712 138
Fragments arranged by size:
1952 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2045 2077 5643 5675 2045
Size: 32 3566 32 2053
Fragments arranged by size:
3566 2053 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5683
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 2527 2708 3008 1870
Size: 657 181 300 4545
Fragments arranged by size:
4545 657 300 181
XmnI GAAnn'nnTTC
Cuts at: 3812 3812
Size: 5683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI SacI SacII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PpuMI PspXI PsrI PvuII RsrII SanDI SapI
SfiI SrfI SwaI XcmI