Restriction map for
pFN21AB7638
(Circular) (Six-base) MAPSORT of: pFN21AB7638.seq Check: 2274 from: 1 to: 5848
pFN21AB7638 5848 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3658 278
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2677 3191 1937
Size: 740 514 4594
Fragments arranged by size:
4594 740 514
Acc65I G'GTAC_C
Cuts at: 3169 3169
Size: 5848
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2478 2725 3911 5295 5563 1632
Size: 846 247 1186 1384 268 1917
Fragments arranged by size:
1917 1384 1186 846 268 247
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5651
AflIII A'CryG_T
Cuts at: 1203 4747 5724 1203
Size: 3544 977 1327
Fragments arranged by size:
3544 1327 977
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 2141 4577 2141
Size: 2436 3412
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2334 1184
Size: 1150 4698
AlwNI CAG_nnn'CTG
Cuts at: 2886 3121 3591 5163 2886
Size: 235 470 1572 3571
Fragments arranged by size:
3571 1572 470 235
ApaI G_GGCC'C
Cuts at: 2152 2333 2847 2969 2152
Size: 181 514 122 5031
Fragments arranged by size:
5031 514 181 122
ApaLI G'TGCA_C
Cuts at: 3905 5061 3905
Size: 1156 4692
ApoI r'AATT_y
Cuts at: 1493 2424 3157 3383 3535 5644 1493
Size: 931 733 226 152 2109 1697
Fragments arranged by size:
2109 1697 931 733 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4402 160
Size: 872 3370 1606
Fragments arranged by size:
3370 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 2264 2622 2754 3173 1870
Size: 73 321 358 132 419 4545
Fragments arranged by size:
4545 419 358 321 132 73
BamHI G'GATC_C
Cuts at: 2669 3178 3545 2669
Size: 509 367 4972
Fragments arranged by size:
4972 509 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3027 3169 4624 618
Size: 325 217 627 1240 142 1455 1842
Fragments arranged by size:
1842 1455 1240 627 325 217 142
BanII G_rGCy'C
Cuts at: 1391 2152 2333 2699 2755 2847 2969 2992 3167
Size: 761 181 366 56 92 122 23 175
Cuts at: 3167 1391
Size: 4072
Fragments arranged by size:
4072 761 366 181 175 122 92 56
23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4877
BbvCI CC'TCA_GC
Cuts at: 2691 2691
Size: 5848
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2171 2205 2222 2256 4039 4073 1903
Size: 34 234 34 17 34 1783 34 3678
Fragments arranged by size:
3678 1783 234 34 34 34 34 17
BciVI GTATCCnnnnn_n'
Cuts at: 3740 4950 3740
Size: 1210 4638
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5848
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2795 2957 4459
Size: 107 122 71 631 605 1123 162 1502
Cuts at: 4459 136
Size: 1525
Fragments arranged by size:
1525 1502 1123 631 605 162 122 107
71
BglII A'GATC_T
Cuts at: 5843 5843
Size: 5848
BlpI GC'TnA_GC
Cuts at: 2366 3265 2366
Size: 899 4949
Bme1580I G_kGCm'C
Cuts at: 1790 2152 2227 2333 2847 2907 2969 3909 5065
Size: 362 75 106 514 60 62 940 1156
Cuts at: 5065 1790
Size: 2573
Fragments arranged by size:
2573 1156 940 514 362 106 75 62
60
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5848
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3579 4537 442
Size: 416 53 83 399 24 2162 958 1753
Fragments arranged by size:
2162 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5848
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 2495 2543 2683 4508 1239
Size: 908 348 48 140 1825 2579
Fragments arranged by size:
2579 1825 908 348 140 48
Bpu10I CC'TnA_GC
Cuts at: 2691 2691
Size: 5848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3025 3128 3973 4838 5136 3025
Size: 103 845 865 298 3737
Fragments arranged by size:
3737 865 845 298 103
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2405 3062 4511 882
Size: 617 906 657 1449 2219
Fragments arranged by size:
2219 1449 906 657 617
BsaAI yAC'GTr
Cuts at: 493 1204 2687 3639 493
Size: 711 1483 952 2702
Fragments arranged by size:
2702 1483 952 711
BsaBI GATnn'nnATC
Cuts at: 5842 5842
Size: 5848
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3655 4037 4672 275
Size: 53 83 186 936 2122 382 635 1451
Fragments arranged by size:
2122 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4281 4679 4953 5100 5511 4281
Size: 398 274 147 411 4618
Fragments arranged by size:
4618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2493 2516 2523 2546 1178
Size: 30 165 30 1090 23 7 23 4480
Fragments arranged by size:
4480 1090 165 30 30 23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 2477 2738 2741 2880 3010 1186
Size: 964 327 261 3 139 130 4024
Fragments arranged by size:
4024 964 327 261 139 130 3
BseYI C'CCAG_C
Cuts at: 2192 2433 3079 5051 5547 2192
Size: 241 646 1972 496 2493
Fragments arranged by size:
2493 1972 646 496 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2195 1163
Size: 573 459 4816
Fragments arranged by size:
4816 573 459
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3298 4059 5087 1540
Size: 275 186 1297 761 1028 2301
Fragments arranged by size:
2301 1297 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2859 3909 3994 5065 2859
Size: 1050 85 1071 3642
Fragments arranged by size:
3642 1071 1050 85
BsmI GAATG_Cn'
Cuts at: 3359 3452 3359
Size: 93 5755
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2227 2333 2699 2755 2847 2859
Size: 399 362 75 106 366 56 92 12
Cuts at: 2859 2907 2969 2992 3167 3909 3994 5065 1391
Size: 48 62 23 175 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 366 362 175 106
92 85 75 62 56 48 23 12
BspEI T'CCGG_A
Cuts at: 5511 5511
Size: 5848
BspHI T'CATG_A
Cuts at: 3737 5467 3737
Size: 1730 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3007 3203 844
Size: 2163 196 3489
Fragments arranged by size:
3489 2163 196
BsrBI CCG'CTC
Cuts at: 1394 1823 2491 2870 3735 1394
Size: 429 668 379 865 3507
Fragments arranged by size:
3507 865 668 429 379
BsrDI GCAATG_nn'
Cuts at: 65 1248 4343 4517 65
Size: 1183 3095 174 1396
Fragments arranged by size:
3095 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2058 4492 4679 1700
Size: 110 248 2434 187 2869
Fragments arranged by size:
2869 2434 248 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5848
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5848
BssSI C'ACGA_G
Cuts at: 3908 4920 3908
Size: 1012 4836
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2312 794
Size: 1518 4330
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5848
BstYI r'GATC_y
Cuts at: 1972 2669 3178 3545 3930 3947 5399 5485 5497
Size: 697 509 367 385 17 1452 86 12
Cuts at: 5497 5843 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 697 509 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2073 2293 2385 2940 513
Size: 552 1008 220 92 555 3421
Fragments arranged by size:
3421 1008 555 552 220 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5109
Fragments arranged by size:
5109 683 56
BtsI GCAGTG_nn'
Cuts at: 2207 2306 3105 3364 4158 4178 2207
Size: 99 799 259 794 20 3877
Fragments arranged by size:
3877 799 794 259 99 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5848
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5813
DraI TTT'AAA
Cuts at: 3152 3152
Size: 5848
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4855 809
Size: 507 3539 1802
Fragments arranged by size:
3539 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2419 2958 3295
Size: 54 1425 50 275 18 589 539 337
Cuts at: 3295 4184 5813 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 589 539 337 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3295 1537
Size: 275 1483 4090
Fragments arranged by size:
4090 1483 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2454 2744 2763 2847 3778 1353
Size: 1101 290 19 84 931 3423
Fragments arranged by size:
3423 1101 931 290 84 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2371 4435 4809 4955 387
Size: 1064 138 782 2064 374 146 1280
Fragments arranged by size:
2064 1280 1064 782 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2478 2495 2543 2683 2725 3911
Size: 393 515 331 17 48 140 42 1186
Cuts at: 3911 4508 5295 5563 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1186 787 597 515 393 331 268
140 48 42 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2922 3113 2922
Size: 191 5657
EcoO109I rG'GnC_Cy
Cuts at: 2155 2329 2438 2929 2965 2966 3118 3139 2155
Size: 174 109 491 36 1 152 21 4864
Fragments arranged by size:
4864 491 174 152 109 36 21 1
EcoRI G'AATT_C
Cuts at: 3157 3535 3157
Size: 378 5470
FspI TGC'GCA
Cuts at: 4354 4354
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 4995 1678
Size: 3317 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2127 2159 2465 2493 2497
Size: 32 135 32 555 32 306 28 4
Cuts at: 2497 2525 4489 4521 4563 4595 1373
Size: 28 1964 32 42 32 2626
Fragments arranged by size:
2626 1964 555 306 135 42 32 32
32 32 32 28 28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2678 3192 3438 669
Size: 988 281 740 514 246 3079
Fragments arranged by size:
3079 988 740 514 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5848
HpaI GTT'AAC
Cuts at: 3438 3438
Size: 5848
KpnI G_GTAC'C
Cuts at: 3173 3173
Size: 5848
MfeI C'AATT_G
Cuts at: 3447 3447
Size: 5848
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4962 5146 1305
Size: 3657 184 2007
Fragments arranged by size:
3657 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2421 2960 10
Size: 54 1425 343 589 539 2898
Fragments arranged by size:
2898 1425 589 539 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3806 4165 4324 518
Size: 648 2640 359 159 2042
Fragments arranged by size:
2640 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2362 2373 2734 3024 3264 3941 5089 5334
Size: 756 11 361 290 240 677 1148 245
Cuts at: 5334 1606
Size: 2120
Fragments arranged by size:
2120 1148 756 677 361 290 245 240
11
NaeI GCC'GGC
Cuts at: 2060 2060
Size: 5848
NcoI C'CATG_G
Cuts at: 513 1065 2073 2293 2385 2940 513
Size: 552 1008 220 92 555 3421
Fragments arranged by size:
3421 1008 555 552 220 92
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5848
NgoMIV G'CCGG_C
Cuts at: 2058 2058
Size: 5848
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5848
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 2315 2633 3206 4751 1132
Size: 97 1086 318 573 1545 2229
Fragments arranged by size:
2229 1545 1086 573 318 97
PasI CC'CwG_GG
Cuts at: 1348 2445 2847 2918 1348
Size: 1097 402 71 4278
Fragments arranged by size:
4278 1097 402 71
PciI A'CATG_T
Cuts at: 4747 4747
Size: 5848
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2428 1108
Size: 1320 4528
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PmlI CAC'GTG
Cuts at: 2687 2687
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 5430 5462 3917
Size: 32 1481 32 4303
Fragments arranged by size:
4303 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2438 2929 2438
Size: 491 5357
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5848
PsiI TTA'TAA
Cuts at: 3418 3418
Size: 5848
PspOMI G'GGCC_C
Cuts at: 2148 2329 2843 2965 2148
Size: 181 514 122 5031
Fragments arranged by size:
5031 514 181 122
PstI C_TGCA'G
Cuts at: 830 2134 2950 3048 3200 830
Size: 1304 816 98 152 3478
Fragments arranged by size:
3478 1304 816 152 98
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
SalI G'TCGA_C
Cuts at: 1936 2676 3190 1936
Size: 740 514 4594
Fragments arranged by size:
4594 740 514
SanDI GG'GwC_CC
Cuts at: 2438 2929 2438
Size: 491 5357
SapI GCTCTTCn'nnn_
Cuts at: 2744 2763 2847 2744
Size: 19 84 5745
Fragments arranged by size:
5745 84 19
SbfI CC_TGCA'GG
Cuts at: 3200 3200
Size: 5848
ScaI AGT'ACT
Cuts at: 4096 5808 4096
Size: 1712 4136
SfcI C'TryA_G
Cuts at: 826 1045 2130 2946 3044 3196 4331 5012 5203
Size: 219 1085 816 98 152 1135 681 191
Cuts at: 5203 826
Size: 1471
Fragments arranged by size:
1471 1135 1085 816 681 219 191 152
98
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5848
SmaI CCC'GGG
Cuts at: 1872 2624 2756 3175 1872
Size: 752 132 419 4545
Fragments arranged by size:
4545 752 419 132
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3004 3143 3952 4853 5115 820
Size: 197 926 1061 139 809 901 262 1553
Fragments arranged by size:
1553 1061 926 901 809 262 197 139
SnaBI TAC'GTA
Cuts at: 493 3639 493
Size: 3146 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5848
SphI G_CATG'C
Cuts at: 1132 2315 3206 1132
Size: 1183 891 3774
Fragments arranged by size:
3774 1183 891
SrfI GCCC'GGGC
Cuts at: 2756 2756
Size: 5848
SspI AAT'ATT
Cuts at: 5 52 3772 5
Size: 47 3720 2081
Fragments arranged by size:
3720 2081 47
StuI AGG'CCT
Cuts at: 1517 2178 1517
Size: 661 5187
StyI C'CwwG_G
Cuts at: 513 1065 2073 2293 2385 2484 2760 2940 3287
Size: 552 1008 220 92 99 276 180 347
Cuts at: 3287 513
Size: 3074
Fragments arranged by size:
3074 1008 552 347 276 220 180 99
92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2187 2832 3875 3892 4045 4230 4675
Size: 1255 607 645 1043 17 153 185 445
Cuts at: 4675 325
Size: 1498
Fragments arranged by size:
1498 1255 1043 645 607 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4094 5806 96
Size: 274 80 33 51 1443 2117 1712 138
Fragments arranged by size:
2117 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2516 2548 5808 5840 2516
Size: 32 3260 32 2524
Fragments arranged by size:
3260 2524 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5848
XbaI T'CTAG_A
Cuts at: 3184 3184
Size: 5848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2071 2291 2071
Size: 220 5628
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5848
XmaI C'CCGG_G
Cuts at: 1870 2622 2754 3173 1870
Size: 752 132 419 4545
Fragments arranged by size:
4545 752 419 132
XmnI GAAnn'nnTTC
Cuts at: 3977 3977
Size: 5848
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3656 276
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PspXI PsrI PvuII RsrII SacI SacII SexAI
SfiI SfoI SwaI