Restriction map for
pFN21AB7636
(Circular) (Six-base) MAPSORT of: pFN21AB7636.seq Check: 7843 from: 1 to: 5836
pFN21AB7636 5836 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3646 278
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2757 3179 1937
Size: 820 422 4594
Fragments arranged by size:
4594 820 422
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 3963 4336 1563
Size: 2400 373 3063
Fragments arranged by size:
3063 2400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2340 3143 3899 5283 5551 1632
Size: 708 803 756 1384 268 1917
Fragments arranged by size:
1917 1384 803 756 708 268
AflII C'TTAA_G
Cuts at: 820 1017 2867 820
Size: 197 1850 3789
Fragments arranged by size:
3789 1850 197
AflIII A'CryG_T
Cuts at: 1203 2643 4735 5712 1203
Size: 1440 2092 977 1327
Fragments arranged by size:
2092 1440 1327 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AlwNI CAG_nnn'CTG
Cuts at: 2044 2318 3579 5151 2044
Size: 274 1261 1572 2729
Fragments arranged by size:
2729 1572 1261 274
ApaLI G'TGCA_C
Cuts at: 3893 5049 3893
Size: 1156 4680
ApoI r'AATT_y
Cuts at: 1493 2507 2609 3145 3371 3523 5632 1493
Size: 1014 102 536 226 152 2109 1697
Fragments arranged by size:
2109 1697 1014 536 226 152 102
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 3161 1870
Size: 73 126 1092 4545
Fragments arranged by size:
4545 1092 126 73
AvrII C'CTAG_G
Cuts at: 2659 2971 2659
Size: 312 5524
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2665 2698 2665
Size: 33 5803
BamHI G'GATC_C
Cuts at: 2818 3166 3533 2818
Size: 348 367 5121
Fragments arranged by size:
5121 367 348
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3157 4612 618
Size: 325 217 627 1370 1455 1842
Fragments arranged by size:
1842 1455 1370 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3081 3155 1391
Size: 1690 74 4072
Fragments arranged by size:
4072 1690 74
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2568 928
Size: 971 669 4196
Fragments arranged by size:
4196 971 669
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4027 4061 1903
Size: 34 2090 34 3678
Fragments arranged by size:
3678 2090 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3728 4938 3728
Size: 1210 4626
BclI T'GATC_A
Cuts at: 1548 2269 1548
Size: 721 5115
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2259 2936 4447
Size: 107 122 71 631 605 587 677 1511
Cuts at: 4447 136
Size: 1525
Fragments arranged by size:
1525 1511 677 631 605 587 122 107
71
BglII A'GATC_T
Cuts at: 2730 5831 2730
Size: 3101 2735
BlpI GC'TnA_GC
Cuts at: 3253 3253
Size: 5836
Bme1580I G_kGCm'C
Cuts at: 1790 2694 3897 5053 1790
Size: 904 1203 1156 2573
Fragments arranged by size:
2573 1203 1156 904
BmgBI CAC'GTC
Cuts at: 1318 2644 1318
Size: 1326 4510
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3567 4525 442
Size: 416 53 83 399 24 2150 958 1753
Fragments arranged by size:
2150 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2439 2829 4496 1239
Size: 986 214 390 1667 2579
Fragments arranged by size:
2579 1667 986 390 214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3961 4826 5124 3961
Size: 865 298 4673
Fragments arranged by size:
4673 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4499 882
Size: 617 3000 2219
Fragments arranged by size:
3000 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2527 3627 493
Size: 711 1323 1100 2702
Fragments arranged by size:
2702 1323 1100 711
BsaBI GATnn'nnATC
Cuts at: 2226 2268 2271 2535 5830 2226
Size: 42 3 264 3295 2232
Fragments arranged by size:
3295 2232 264 42 3
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3643 4025 4660 275
Size: 53 83 186 936 2110 382 635 1451
Fragments arranged by size:
2110 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2275 4269 4667 4941 5088 5499 2275
Size: 1994 398 274 147 411 2612
Fragments arranged by size:
2612 1994 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2541 2571 2597 2627 1178
Size: 30 165 30 1138 30 26 30 4387
Fragments arranged by size:
4387 1138 165 30 30 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2213 2925 2928 1186
Size: 838 189 712 3 4094
Fragments arranged by size:
4094 838 712 189 3
BseYI C'CCAG_C
Cuts at: 2311 2794 2813 5039 5535 2311
Size: 483 19 2226 496 2612
Fragments arranged by size:
2612 2226 496 483 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2525 2588 1163
Size: 573 789 63 4411
Fragments arranged by size:
4411 789 573 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3286 4047 5075 1540
Size: 275 186 1285 761 1028 2301
Fragments arranged by size:
2301 1285 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2831 3897 3982 5053 2831
Size: 1066 85 1071 3614
Fragments arranged by size:
3614 1071 1066 85
BsmI GAATG_Cn'
Cuts at: 2128 2567 3347 3440 2128
Size: 439 780 93 4524
Fragments arranged by size:
4524 780 439 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2694 2831 3081 3155 3897 3982 5053
Size: 399 904 137 250 74 742 85 1071
Cuts at: 5053 1391
Size: 2174
Fragments arranged by size:
2174 1071 904 742 399 250 137 85
74
BspEI T'CCGG_A
Cuts at: 2275 5499 2275
Size: 3224 2612
BspHI T'CATG_A
Cuts at: 2589 3004 3725 5455 2589
Size: 415 721 1730 2970
Fragments arranged by size:
2970 1730 721 415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3191 844
Size: 2347 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3723 1394
Size: 429 1900 3507
Fragments arranged by size:
3507 1900 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3079 4331 4505 65
Size: 1183 1831 1252 174 1396
Fragments arranged by size:
1831 1396 1252 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2023 3060 4480 4667 1700
Size: 110 213 1037 1420 187 2869
Fragments arranged by size:
2869 1420 1037 213 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5836
BssSI C'ACGA_G
Cuts at: 3896 4908 3896
Size: 1012 4824
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5836
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5836
BstXI CCAn_nnnn'nTGG
Cuts at: 2552 2782 2552
Size: 230 5606
BstYI r'GATC_y
Cuts at: 1972 2103 2536 2730 2818 2994 3166 3533 3918
Size: 131 433 194 88 176 172 367 385
Cuts at: 3918 3935 5387 5473 5485 5831 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 433 385 367 346 194 176
172 131 88 86 17 12
BstZ17I GTA'TAC
Cuts at: 2758 2758
Size: 5836
BtgI C'CryG_G
Cuts at: 513 1065 2004 2193 2545 513
Size: 552 939 189 352 3804
Fragments arranged by size:
3804 939 552 352 189
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2277 487
Size: 683 56 1051 4046
Fragments arranged by size:
4046 1051 683 56
BtsI GCAGTG_nn'
Cuts at: 2150 2787 3352 4146 4166 2150
Size: 637 565 794 20 3820
Fragments arranged by size:
3820 794 637 565 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5801
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 809
Size: 507 3527 1802
Fragments arranged by size:
3527 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2205 3062
Size: 54 1425 50 275 18 267 108 857
Cuts at: 3062 3283 4172 5801 8
Size: 221 889 1629 43
Fragments arranged by size:
1629 1425 889 857 275 267 221 108
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3283 1537
Size: 275 1471 4090
Fragments arranged by size:
4090 1471 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3766 1353
Size: 2413 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 4423 4797 4943 387
Size: 1064 138 453 2381 374 146 1280
Fragments arranged by size:
2381 1280 1064 453 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2340 2439 2829 3143 3899 4496
Size: 393 593 115 99 390 314 756 597
Cuts at: 4496 5283 5551 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 756 597 593 393 390 314
268 115 99
EcoNI CCTnn'n_nnAGG
Cuts at: 2663 2663
Size: 5836
EcoRI G'AATT_C
Cuts at: 3145 3523 3145
Size: 378 5458
FspI TGC'GCA
Cuts at: 4342 4342
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 4983 1678
Size: 3305 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2435 2467 2986 3018 4477
Size: 32 135 32 863 32 519 32 1459
Cuts at: 4477 4509 4551 4583 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1459 863 519 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3180 3426 669
Size: 988 281 1242 246 3079
Fragments arranged by size:
3079 1242 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5836
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 3435 3435
Size: 5836
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3115 4950 5134 1305
Size: 1810 1835 184 2007
Fragments arranged by size:
2007 1835 1810 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2099 2207 10
Size: 54 1425 343 267 108 3639
Fragments arranged by size:
3639 1425 343 267 108 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2220 2550 2594 3794 4153 4312 518
Size: 648 1054 330 44 1200 359 159 2042
Fragments arranged by size:
2042 1200 1054 648 359 330 159 44
MspA1I CmG'CkG
Cuts at: 1606 2369 2794 3252 3929 5077 5322 1606
Size: 763 425 458 677 1148 245 2120
Fragments arranged by size:
2120 1148 763 677 458 425 245
NaeI GCC'GGC
Cuts at: 3062 3062
Size: 5836
NcoI C'CATG_G
Cuts at: 513 1065 2004 2193 2545 513
Size: 552 939 189 352 3804
Fragments arranged by size:
3804 939 552 352 189
NdeI CA'TA_TG
Cuts at: 387 2146 3022 387
Size: 1759 876 3201
Fragments arranged by size:
3201 1759 876
NgoMIV G'CCGG_C
Cuts at: 3060 3060
Size: 5836
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 3131 3194 4739 1132
Size: 97 1902 63 1545 2229
Fragments arranged by size:
2229 1902 1545 97 63
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5836
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5836
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 5418 5450 3905
Size: 32 1481 32 4291
Fragments arranged by size:
4291 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2963 1613
Size: 1350 4486
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PstI C_TGCA'G
Cuts at: 830 2235 3188 830
Size: 1405 953 3478
Fragments arranged by size:
3478 1405 953
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
PvuII CAG'CTG
Cuts at: 2369 2794 2369
Size: 425 5411
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SbfI CC_TGCA'GG
Cuts at: 3188 3188
Size: 5836
ScaI AGT'ACT
Cuts at: 4084 5796 4084
Size: 1712 4124
SfcI C'TryA_G
Cuts at: 826 1045 2062 2231 2346 2677 3184 4319 5000
Size: 219 1017 169 115 331 507 1135 681
Cuts at: 5000 5191 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1017 681 507 331 219 191
169 115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 3163 1872
Size: 1291 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2867 3940 4841 5103 820
Size: 197 926 924 1073 901 262 1553
Fragments arranged by size:
1553 1073 926 924 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 3194 1132
Size: 2062 3774
SspI AAT'ATT
Cuts at: 5 52 2490 2723 3760 5
Size: 47 2438 233 1037 2081
Fragments arranged by size:
2438 2081 1037 233 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5836
StyI C'CwwG_G
Cuts at: 513 1065 2004 2193 2545 2659 2971 3275 513
Size: 552 939 189 352 114 312 304 3074
Fragments arranged by size:
3074 939 552 352 312 304 189 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3863 3880 4033 4218 4663 325
Size: 1255 2283 17 153 185 445 1498
Fragments arranged by size:
2283 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2743 2871 4082
Size: 274 80 33 51 1443 766 128 1211
Cuts at: 4082 5794 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1211 766 274 138 128 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2597 2629 2810 2842 5796 5828 2597
Size: 32 181 32 2954 32 2605
Fragments arranged by size:
2954 2605 181 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2596 1316
Size: 1280 4556
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2200 2200
Size: 5836
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5836
XmaI C'CCGG_G
Cuts at: 1870 3161 1870
Size: 1291 4545
XmnI GAAnn'nnTTC
Cuts at: 3965 3965
Size: 5836
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3644 276
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI