(Circular) (Six-base) MAPSORT of: pFN21AB7633.seq Check: 9588 from: 1 to: 5668 pFN21AB7633 5668 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3478 278 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3011 1937 Size: 1074 4594 Acc65I G'GTAC_C Cuts at: 2989 2989 Size: 5668 AclI AA'CG_TT Cuts at: 1563 3795 4168 1563 Size: 2232 373 3063 Fragments arranged by size: 3063 2232 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2237 2573 2722 3731 5115 5383 1632 Size: 605 336 149 1009 1384 268 1917 Fragments arranged by size: 1917 1384 1009 605 336 268 149 AflII C'TTAA_G Cuts at: 820 1017 2420 2672 820 Size: 197 1403 252 3816 Fragments arranged by size: 3816 1403 252 197 AflIII A'CryG_T Cuts at: 1203 2596 2848 4567 5544 1203 Size: 1393 252 1719 977 1327 Fragments arranged by size: 1719 1393 1327 977 252 AgeI A'CCGG_T Cuts at: 4499 4499 Size: 5668 AhdI GACnn_n'nnGTC Cuts at: 4397 4397 Size: 5668 AleI CACnn'nnGTG Cuts at: 1166 2781 1166 Size: 1615 4053 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5668 AlwNI CAG_nnn'CTG Cuts at: 2388 2640 3411 4983 2388 Size: 252 771 1572 3073 Fragments arranged by size: 3073 1572 771 252 ApaLI G'TGCA_C Cuts at: 2510 2762 3725 4881 2510 Size: 252 963 1156 3297 Fragments arranged by size: 3297 1156 963 252 ApoI r'AATT_y Cuts at: 1493 2235 2827 2977 3203 3355 5464 1493 Size: 742 592 150 226 152 2109 1697 Fragments arranged by size: 2109 1697 742 592 226 152 150 AseI AT'TA_AT Cuts at: 160 1032 4222 160 Size: 872 3190 1606 Fragments arranged by size: 3190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 AvaI C'yCGr_G Cuts at: 1870 1943 2993 1870 Size: 73 1050 4545 Fragments arranged by size: 4545 1050 73 BamHI G'GATC_C Cuts at: 2998 3365 2998 Size: 367 5301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2989 4444 618 Size: 325 217 627 1202 1455 1842 Fragments arranged by size: 1842 1455 1202 627 325 217 BanII G_rGCy'C Cuts at: 1391 2498 2987 1391 Size: 1107 489 4072 Fragments arranged by size: 4072 1107 489 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2225 2406 928 Size: 971 326 181 4190 Fragments arranged by size: 4190 971 326 181 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3859 3893 1903 Size: 34 1922 34 3678 Fragments arranged by size: 3678 1922 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3560 4770 3560 Size: 1210 4458 BclI T'GATC_A Cuts at: 1548 2467 1548 Size: 919 4749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4279 136 Size: 107 122 71 631 605 2607 1525 Fragments arranged by size: 2607 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2435 5663 2435 Size: 3228 2440 BlpI GC'TnA_GC Cuts at: 3085 3085 Size: 5668 Bme1580I G_kGCm'C Cuts at: 1790 2514 2766 3729 4885 1790 Size: 724 252 963 1156 2573 Fragments arranged by size: 2573 1156 963 724 252 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5668 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2269 3399 4357 Size: 416 53 83 399 24 852 1130 958 Cuts at: 4357 442 Size: 1753 Fragments arranged by size: 1753 1130 958 852 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5668 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2382 2466 2550 2634 2718 2886 4328 Size: 917 226 84 84 84 84 168 1442 Cuts at: 4328 1239 Size: 2579 Fragments arranged by size: 2579 1442 917 226 168 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3793 4658 4956 3793 Size: 865 298 4505 Fragments arranged by size: 4505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4331 882 Size: 617 2832 2219 Fragments arranged by size: 2832 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2851 3459 493 Size: 711 1647 608 2702 Fragments arranged by size: 2702 1647 711 608 BsaBI GATnn'nnATC Cuts at: 5662 5662 Size: 5668 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3475 3857 4492 275 Size: 53 83 186 936 1942 382 635 1451 Fragments arranged by size: 1942 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4101 4499 4773 4920 5331 4101 Size: 398 274 147 411 4438 Fragments arranged by size: 4438 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5443 Fragments arranged by size: 5443 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 1186 Size: 910 4758 BseYI C'CCAG_C Cuts at: 4871 5367 4871 Size: 496 5172 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2274 1163 Size: 573 538 4557 Fragments arranged by size: 4557 573 538 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3118 3879 4907 1540 Size: 275 186 1117 761 1028 2301 Fragments arranged by size: 2301 1117 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2498 2514 2766 3729 3814 4885 2498 Size: 16 252 963 85 1071 3281 Fragments arranged by size: 3281 1071 963 252 85 16 BsmI GAATG_Cn' Cuts at: 2384 2636 3179 3272 2384 Size: 252 543 93 4780 Fragments arranged by size: 4780 543 252 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2498 2514 2766 2987 3729 3814 4885 Size: 399 708 16 252 221 742 85 1071 Cuts at: 4885 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 708 399 252 221 85 16 BspEI T'CCGG_A Cuts at: 5331 5331 Size: 5668 BspHI T'CATG_A Cuts at: 3557 5287 3557 Size: 1730 3938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3023 844 Size: 2179 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3555 1394 Size: 429 1732 3507 Fragments arranged by size: 3507 1732 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2090 4163 4337 65 Size: 1183 842 2073 174 1396 Fragments arranged by size: 2073 1396 1183 842 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4312 4499 1700 Size: 110 2502 187 2869 Fragments arranged by size: 2869 2502 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5668 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5668 BssSI C'ACGA_G Cuts at: 3728 4740 3728 Size: 1012 4656 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5668 BstBI TT'CG_AA Cuts at: 4472 4472 Size: 5668 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5668 BstYI r'GATC_y Cuts at: 1972 2435 2998 3365 3750 3767 5219 5305 5317 Size: 463 563 367 385 17 1452 86 12 Cuts at: 5317 5663 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 563 463 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2840 2840 Size: 5668 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4177 Fragments arranged by size: 4177 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4929 Fragments arranged by size: 4929 683 56 BtsI GCAGTG_nn' Cuts at: 2070 2389 2641 3184 3978 3998 2070 Size: 319 252 543 794 20 3740 Fragments arranged by size: 3740 794 543 319 252 20 ClaI AT'CG_AT Cuts at: 1554 2658 1554 Size: 1104 4564 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5633 DraI TTT'AAA Cuts at: 2972 2972 Size: 5668 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2029 4675 809 Size: 507 713 2646 1802 Fragments arranged by size: 2646 1802 713 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2950 3115 4004 Size: 54 1425 50 275 18 1120 165 889 Cuts at: 4004 5633 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1120 889 275 165 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3115 1537 Size: 275 1303 4090 Fragments arranged by size: 4090 1303 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3598 1353 Size: 2245 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4255 4629 4775 387 Size: 1064 138 2666 374 146 1280 Fragments arranged by size: 2666 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2496 2496 Size: 5668 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2237 2382 2466 2550 2573 2634 Size: 393 524 81 145 84 84 23 61 Cuts at: 2634 2718 2722 2886 3731 4328 5115 5383 1239 Size: 84 4 164 845 597 787 268 1524 Fragments arranged by size: 1524 845 787 597 524 393 268 164 145 84 84 84 81 61 23 4 EcoRI G'AATT_C Cuts at: 2827 2977 3355 2827 Size: 150 378 5140 Fragments arranged by size: 5140 378 150 FspI TGC'GCA Cuts at: 4174 4174 Size: 5668 HaeII r_GCGC'y Cuts at: 1678 4815 1678 Size: 3137 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2485 2517 2651 2683 4309 Size: 32 135 32 913 32 134 32 1626 Cuts at: 4309 4341 4383 4415 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1626 913 135 134 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3012 3258 669 Size: 988 281 1074 246 3079 Fragments arranged by size: 3079 1074 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5668 HpaI GTT'AAC Cuts at: 3258 3258 Size: 5668 KpnI G_GTAC'C Cuts at: 2993 2993 Size: 5668 MfeI C'AATT_G Cuts at: 2289 3267 2289 Size: 978 4690 MluI A'CGCG_T Cuts at: 5544 5544 Size: 5668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4782 4966 1305 Size: 3477 184 2007 Fragments arranged by size: 3477 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2952 10 Size: 54 1425 343 1120 2726 Fragments arranged by size: 2726 1425 1120 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2505 2781 3626 3985 4144 518 Size: 648 1339 276 845 359 159 2042 Fragments arranged by size: 2042 1339 845 648 359 276 159 MspA1I CmG'CkG Cuts at: 1606 2215 3084 3761 4909 5154 1606 Size: 609 869 677 1148 245 2120 Fragments arranged by size: 2120 1148 869 677 609 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4177 Fragments arranged by size: 4177 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5668 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5668 NotI GC'GGCC_GC Cuts at: 3115 3115 Size: 5668 NspI r_CATG'y Cuts at: 1132 1229 2600 3026 4571 1132 Size: 97 1371 426 1545 2229 Fragments arranged by size: 2229 1545 1371 426 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5668 PciI A'CATG_T Cuts at: 2596 4567 2596 Size: 1971 3697 PflMI CCAn_nnn'nTGG Cuts at: 1108 2059 1108 Size: 951 4717 PfoI T'CCnGG_A Cuts at: 2052 2052 Size: 5668 PmeI GTTT'AAAC Cuts at: 2972 2972 Size: 5668 PmlI CAC'GTG Cuts at: 2851 2851 Size: 5668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2141 2173 3737 3769 5250 5282 2141 Size: 32 1564 32 1481 32 2527 Fragments arranged by size: 2527 1564 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5668 PsiI TTA'TAA Cuts at: 2880 2927 3238 2880 Size: 47 311 5310 Fragments arranged by size: 5310 311 47 PstI C_TGCA'G Cuts at: 830 2014 2180 3020 830 Size: 1184 166 840 3478 Fragments arranged by size: 3478 1184 840 166 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5668 PvuII CAG'CTG Cuts at: 2215 2215 Size: 5668 SacI G_AGCT'C Cuts at: 2498 2498 Size: 5668 SalI G'TCGA_C Cuts at: 1936 3010 1936 Size: 1074 4594 SbfI CC_TGCA'GG Cuts at: 3020 3020 Size: 5668 ScaI AGT'ACT Cuts at: 3916 5628 3916 Size: 1712 3956 SexAI A'CCwGG_T Cuts at: 2027 2027 Size: 5668 SfcI C'TryA_G Cuts at: 826 1045 2010 2176 3016 4151 4832 5023 826 Size: 219 965 166 840 1135 681 191 1471 Fragments arranged by size: 1471 1135 965 840 681 219 191 166 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5668 SmaI CCC'GGG Cuts at: 1872 2995 1872 Size: 1123 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2420 2672 3772 4673 4935 820 Size: 197 926 477 252 1100 901 262 1553 Fragments arranged by size: 1553 1100 926 901 477 262 252 197 SnaBI TAC'GTA Cuts at: 493 3459 493 Size: 2966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5668 SphI G_CATG'C Cuts at: 1132 3026 1132 Size: 1894 3774 SspI AAT'ATT Cuts at: 5 52 3592 5 Size: 47 3540 2081 Fragments arranged by size: 3540 2081 47 StuI AGG'CCT Cuts at: 1517 2819 1517 Size: 1302 4366 StyI C'CwwG_G Cuts at: 513 1065 2004 2196 3107 513 Size: 552 939 192 911 3074 Fragments arranged by size: 3074 939 911 552 192 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3695 3712 3865 4050 4495 325 Size: 1255 2115 17 153 185 445 1498 Fragments arranged by size: 2115 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3914 5626 96 Size: 274 80 33 51 1443 1937 1712 138 Fragments arranged by size: 1937 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5628 5660 5628 Size: 32 5636 Tth111I GACn'n_nGTC Cuts at: 1316 2184 1316 Size: 868 4800 XbaI T'CTAG_A Cuts at: 3004 3004 Size: 5668 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5668 XmaI C'CCGG_G Cuts at: 1870 2993 1870 Size: 1123 4545 XmnI GAAnn'nnTTC Cuts at: 2399 2567 3797 2399 Size: 168 1230 4270 Fragments arranged by size: 4270 1230 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3476 276 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI