Restriction map for
pFN21AB7632
(Circular) (Six-base) MAPSORT of: pFN21AB7632.seq Check: 1139 from: 1 to: 5797
pFN21AB7632 5797 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2411 3140 1937
Size: 474 729 4594
Fragments arranged by size:
4594 729 474
Acc65I G'GTAC_C
Cuts at: 3118 3118
Size: 5797
AclI AA'CG_TT
Cuts at: 1563 2435 2697 3924 4297 1563
Size: 872 262 1227 373 3063
Fragments arranged by size:
3063 1227 872 373 262
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3860 5244 5512 1632
Size: 2228 1384 268 1917
Fragments arranged by size:
2228 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2290 820
Size: 197 1273 4327
Fragments arranged by size:
4327 1273 197
AflIII A'CryG_T
Cuts at: 1203 4696 5673 1203
Size: 3493 977 1327
Fragments arranged by size:
3493 1327 977
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 2892 4526 2892
Size: 1634 4163
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5797
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2072 2104 2072
Size: 32 5765
AlwNI CAG_nnn'CTG
Cuts at: 3540 5112 3540
Size: 1572 4225
ApaI G_GGCC'C
Cuts at: 2487 2634 3026 3066 2487
Size: 147 392 40 5218
Fragments arranged by size:
5218 392 147 40
ApaLI G'TGCA_C
Cuts at: 3854 5010 3854
Size: 1156 4641
ApoI r'AATT_y
Cuts at: 1493 3106 3332 3484 5593 1493
Size: 1613 226 152 2109 1697
Fragments arranged by size:
2109 1697 1613 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 2582 2627 2943 3066 3122 1870
Size: 73 639 45 316 123 56 4545
Fragments arranged by size:
4545 639 316 123 73 56 45
BamHI G'GATC_C
Cuts at: 3127 3494 3127
Size: 367 5430
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 3118 4573 618
Size: 325 217 627 239 1092 1455 1842
Fragments arranged by size:
1842 1455 1092 627 325 239 217
BanII G_rGCy'C
Cuts at: 1391 2487 2634 3026 3048 3066 3116 1391
Size: 1096 147 392 22 18 50 4072
Fragments arranged by size:
4072 1096 392 147 50 22 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2042 928
Size: 971 143 4683
Fragments arranged by size:
4683 971 143
BbvCI CC'TCA_GC
Cuts at: 3013 3013
Size: 5797
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3988 4022 1903
Size: 34 2051 34 3678
Fragments arranged by size:
3678 2051 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4408 136
Size: 107 122 71 631 605 2736 1525
Fragments arranged by size:
2736 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 2418 3214 2418
Size: 796 5001
Bme1580I G_kGCm'C
Cuts at: 1790 2029 2487 2627 2634 3026 3066 3858 5014
Size: 239 458 140 7 392 40 792 1156
Cuts at: 5014 1790
Size: 2573
Fragments arranged by size:
2573 1156 792 458 392 239 140 40
7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5797
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3528 4486 442
Size: 416 53 83 399 24 2111 958 1753
Fragments arranged by size:
2111 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2585 4457 1239
Size: 1346 1872 2579
Fragments arranged by size:
2579 1872 1346
Bpu10I CC'TnA_GC
Cuts at: 3013 3042 3013
Size: 29 5768
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2267 3922 4787 5085 2267
Size: 1655 865 298 2979
Fragments arranged by size:
2979 1655 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4460 882
Size: 617 2961 2219
Fragments arranged by size:
2961 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2792 3588 493
Size: 711 1588 796 2702
Fragments arranged by size:
2702 1588 796 711
BsaBI GATnn'nnATC
Cuts at: 2139 5791 2139
Size: 3652 2145
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3604 3986 4621 275
Size: 53 83 186 936 2071 382 635 1451
Fragments arranged by size:
2071 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2126 4230 4628 4902 5049 5460 2126
Size: 2104 398 274 147 411 2463
Fragments arranged by size:
2463 2104 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5572
Fragments arranged by size:
5572 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2736 1186
Size: 904 646 4247
Fragments arranged by size:
4247 904 646
BseYI C'CCAG_C
Cuts at: 2015 2042 2753 5000 5496 2015
Size: 27 711 2247 496 2316
Fragments arranged by size:
2316 2247 711 496 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5224
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 4008 5036 1540
Size: 275 186 1246 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3858 3943 5014 3858
Size: 85 1071 4641
Fragments arranged by size:
4641 1071 85
BsmI GAATG_Cn'
Cuts at: 3308 3401 3308
Size: 93 5704
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2487 2627 2634 3026 3048 3066
Size: 399 239 458 140 7 392 22 18
Cuts at: 3066 3116 3858 3943 5014 1391
Size: 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 458 399 392 239 140
85 50 22 18 7
BspEI T'CCGG_A
Cuts at: 2126 5460 2126
Size: 3334 2463
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2593 3152 844
Size: 1749 559 3489
Fragments arranged by size:
3489 1749 559
BsrBI CCG'CTC
Cuts at: 1394 1823 2497 2578 3684 1394
Size: 429 674 81 1106 3507
Fragments arranged by size:
3507 1106 674 429 81
BsrDI GCAATG_nn'
Cuts at: 65 1248 4292 4466 65
Size: 1183 3044 174 1396
Fragments arranged by size:
3044 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2049 4441 4628 1700
Size: 110 239 2392 187 2869
Fragments arranged by size:
2869 2392 239 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 3857 4869 3857
Size: 1012 4785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5797
BstBI TT'CG_AA
Cuts at: 4601 4601
Size: 5797
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5797
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5797
BstYI r'GATC_y
Cuts at: 1972 3127 3494 3879 3896 5348 5434 5446 5792
Size: 1155 367 385 17 1452 86 12 346
Cuts at: 5792 1972
Size: 1977
Fragments arranged by size:
1977 1452 1155 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2992 513
Size: 552 1927 3318
Fragments arranged by size:
3318 1927 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5058
Fragments arranged by size:
5058 683 56
BtsI GCAGTG_nn'
Cuts at: 2937 3006 3313 4107 4127 2937
Size: 69 307 794 20 4607
Fragments arranged by size:
4607 794 307 69 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4804 809
Size: 507 3488 1802
Fragments arranged by size:
3488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2498 3244 4133
Size: 54 1425 50 275 18 668 746 889
Cuts at: 4133 5762 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 746 668 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 1537
Size: 275 1432 4090
Fragments arranged by size:
4090 1432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2226 2337 2538 2685 3727 1353
Size: 873 111 201 147 1042 3423
Fragments arranged by size:
3423 1042 873 201 147 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4384 4758 4904 387
Size: 1064 138 2795 374 146 1280
Fragments arranged by size:
2795 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2585 3860 4457 5244 5512 1239
Size: 393 953 1275 597 787 268 1524
Fragments arranged by size:
1524 1275 953 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2483 2484 2681 2911 3023 3063 2483
Size: 1 197 230 112 40 5217
Fragments arranged by size:
5217 230 197 112 40 1
EcoRI G'AATT_C
Cuts at: 3106 3484 3106
Size: 378 5419
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 2251 4944 1678
Size: 573 2693 2531
Fragments arranged by size:
2693 2531 573
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 2366 2398 2878
Size: 32 135 32 623 32 139 32 480
Cuts at: 2878 2910 4438 4470 4512 4544 1373
Size: 32 1528 32 42 32 2626
Fragments arranged by size:
2626 1528 623 480 139 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2308 2701 2770 3141 3387 669
Size: 988 281 370 393 69 371 246 3079
Fragments arranged by size:
3079 988 393 371 370 281 246 69
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5797
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KpnI G_GTAC'C
Cuts at: 3122 3122
Size: 5797
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2372 4911 5095 1305
Size: 1067 2539 184 2007
Fragments arranged by size:
2539 2007 1067 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3975
Fragments arranged by size:
3975 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3755 4114 4273 518
Size: 648 2589 359 159 2042
Fragments arranged by size:
2589 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2042 2417 3213 3890 5038 5283 1606
Size: 436 375 796 677 1148 245 2120
Fragments arranged by size:
2120 1148 796 677 436 375 245
NcoI C'CATG_G
Cuts at: 513 1065 2992 513
Size: 552 1927 3318
Fragments arranged by size:
3318 1927 552
NdeI CA'TA_TG
Cuts at: 387 2572 387
Size: 2185 3612
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 3155 4700 1132
Size: 97 1926 1545 2229
Fragments arranged by size:
2229 1926 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3019 1348
Size: 1671 4126
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2669 1108
Size: 1561 4236
PfoI T'CCnGG_A
Cuts at: 2523 2523
Size: 5797
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2819 2851 3866 3898 5379 5411 2819
Size: 32 1015 32 1481 32 3205
Fragments arranged by size:
3205 1481 1015 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2911 2911
Size: 5797
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PspOMI G'GGCC_C
Cuts at: 2483 2630 3022 3062 2483
Size: 147 392 40 5218
Fragments arranged by size:
5218 392 147 40
PspXI vC'TCGA_Gb
Cuts at: 2582 2943 2582
Size: 361 5436
PstI C_TGCA'G
Cuts at: 830 3149 830
Size: 2319 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
PvuII CAG'CTG
Cuts at: 2417 2417
Size: 5797
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SanDI GG'GwC_CC
Cuts at: 2911 2911
Size: 5797
SapI GCTCTTCn'nnn_
Cuts at: 2226 2538 2226
Size: 312 5485
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SfcI C'TryA_G
Cuts at: 826 1045 2412 3145 4280 4961 5152 826
Size: 219 1367 733 1135 681 191 1471
Fragments arranged by size:
1471 1367 1135 733 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 2629 3124 1872
Size: 757 495 4545
Fragments arranged by size:
4545 757 495
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2282 2290 2582 2943 3901 4802
Size: 197 926 339 8 292 361 958 901
Cuts at: 4802 5064 820
Size: 262 1553
Fragments arranged by size:
1553 958 926 901 361 339 292 262
197 8
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 3155 1132
Size: 2023 3774
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 2267 2296 1517
Size: 750 29 5018
Fragments arranged by size:
5018 750 29
StyI C'CwwG_G
Cuts at: 513 1065 2262 2685 2842 2992 3236 513
Size: 552 1197 423 157 150 244 3074
Fragments arranged by size:
3074 1197 552 423 244 157 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3824 3841 3994 4179 4624 325
Size: 1255 2244 17 153 185 445 1498
Fragments arranged by size:
2244 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2979 4043 5755
Size: 274 80 33 51 1443 1002 1064 1712
Cuts at: 5755 96
Size: 138
Fragments arranged by size:
1712 1443 1064 1002 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5757 5789 5757
Size: 32 5765
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5797
XbaI T'CTAG_A
Cuts at: 3133 3133
Size: 5797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2102 2102
Size: 5797
XhoI C'TCGA_G
Cuts at: 1943 2582 2943 1943
Size: 639 361 4797
Fragments arranged by size:
4797 639 361
XmaI C'CCGG_G
Cuts at: 1870 2627 3122 1870
Size: 757 495 4545
Fragments arranged by size:
4545 757 495
XmnI GAAnn'nnTTC
Cuts at: 3926 3926
Size: 5797
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PsrI RsrII SacI SacII SexAI
SfiI SfoI SrfI SwaI