Restriction map for
pFN21AB7631
(Circular) (Six-base) MAPSORT of: pFN21AB7631.seq Check: 5881 from: 1 to: 5665
pFN21AB7631 5665 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3475 278
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2037 3008 1937
Size: 100 971 4594
Fragments arranged by size:
4594 971 100
Acc65I G'GTAC_C
Cuts at: 2986 2986
Size: 5665
AclI AA'CG_TT
Cuts at: 1563 3792 4165 1563
Size: 2229 373 3063
Fragments arranged by size:
3063 2229 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3728 5112 5380 1632
Size: 2096 1384 268 1917
Fragments arranged by size:
2096 1917 1384 268
AfeI AGC'GCT
Cuts at: 2405 2405
Size: 5665
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5468
AflIII A'CryG_T
Cuts at: 1203 2598 4564 5541 1203
Size: 1395 1966 977 1327
Fragments arranged by size:
1966 1395 1327 977
AgeI A'CCGG_T
Cuts at: 4496 4496
Size: 5665
AhdI GACnn_n'nnGTC
Cuts at: 2758 4394 2758
Size: 1636 4029
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2874 2943 1184
Size: 1690 69 3906
Fragments arranged by size:
3906 1690 69
AlwNI CAG_nnn'CTG
Cuts at: 2491 3408 4980 2491
Size: 917 1572 3176
Fragments arranged by size:
3176 1572 917
ApaI G_GGCC'C
Cuts at: 2552 2552
Size: 5665
ApaLI G'TGCA_C
Cuts at: 2210 3722 4878 2210
Size: 1512 1156 2997
Fragments arranged by size:
2997 1512 1156
ApoI r'AATT_y
Cuts at: 1493 2974 3200 3352 5461 1493
Size: 1481 226 152 2109 1697
Fragments arranged by size:
2109 1697 1481 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4219 160
Size: 872 3187 1606
Fragments arranged by size:
3187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2221 2560 2653 2723 2990 1870
Size: 73 278 339 93 70 267 4545
Fragments arranged by size:
4545 339 278 267 93 73 70
BamHI G'GATC_C
Cuts at: 2995 3362 2995
Size: 367 5298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2262 2361 2522 2660 2687
Size: 325 217 627 475 99 161 138 27
Cuts at: 2687 2704 2800 2986 4441 618
Size: 17 96 186 1455 1842
Fragments arranged by size:
1842 1455 627 475 325 217 186 161
138 99 96 27 17
BanII G_rGCy'C
Cuts at: 1391 2552 2795 2984 1391
Size: 1161 243 189 4072
Fragments arranged by size:
4072 1161 243 189
BbeI G_GCGC'C
Cuts at: 2365 2526 2664 2691 2708 2804 2365
Size: 161 138 27 17 96 5226
Fragments arranged by size:
5226 161 138 96 27 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2142 928
Size: 971 243 4451
Fragments arranged by size:
4451 971 243
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2777 2811 3856 3890 1903
Size: 34 840 34 1045 34 3678
Fragments arranged by size:
3678 1045 840 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3557 4767 3557
Size: 1210 4455
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2260 2300 2571
Size: 107 122 71 631 605 588 40 271
Cuts at: 2571 4276 136
Size: 1705 1525
Fragments arranged by size:
1705 1525 631 605 588 271 122 107
71 40
BglII A'GATC_T
Cuts at: 5660 5660
Size: 5665
BlpI GC'TnA_GC
Cuts at: 3082 3082
Size: 5665
Bme1580I G_kGCm'C
Cuts at: 1790 2214 2265 2552 2927 3726 4882 1790
Size: 424 51 287 375 799 1156 2573
Fragments arranged by size:
2573 1156 799 424 375 287 51
BmgBI CAC'GTC
Cuts at: 1318 2412 2824 1318
Size: 1094 412 4159
Fragments arranged by size:
4159 1094 412
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2893 3396 4354
Size: 416 53 83 399 24 1476 503 958
Cuts at: 4354 442
Size: 1753
Fragments arranged by size:
1753 1476 958 503 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5665
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2797 2797
Size: 5665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4325 1239
Size: 3086 2579
Bpu10I CC'TnA_GC
Cuts at: 2369 2369
Size: 5665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2384 3790 4655 4953 2384
Size: 1406 865 298 3096
Fragments arranged by size:
3096 1406 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4328 882
Size: 617 2829 2219
Fragments arranged by size:
2829 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2161 2231 3456 493
Size: 711 957 70 1225 2702
Fragments arranged by size:
2702 1225 957 711 70
BsaBI GATnn'nnATC
Cuts at: 5659 5659
Size: 5665
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2362 2508 2523 2661
Size: 53 83 186 936 829 146 15 138
Cuts at: 2661 2688 2705 2801 2910 3472 3854 4489 275
Size: 27 17 96 109 562 382 635 1451
Fragments arranged by size:
1451 936 829 635 562 382 186 146
138 109 96 83 53 27 17 15
BsaWI w'CCGG_w
Cuts at: 2905 4098 4496 4770 4917 5328 2905
Size: 1193 398 274 147 411 3242
Fragments arranged by size:
3242 1193 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 1178
Size: 30 165 30 597 30 4813
Fragments arranged by size:
4813 597 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5665
BseYI C'CCAG_C
Cuts at: 2255 2708 2867 4868 5364 2255
Size: 453 159 2001 496 2556
Fragments arranged by size:
2556 2001 496 453 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2392 1163
Size: 573 656 4436
Fragments arranged by size:
4436 656 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2332 3115 3876 4904 1540
Size: 275 186 331 783 761 1028 2301
Fragments arranged by size:
2301 1028 783 761 331 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 2214 2580 2964 3726 3811 4882 2190
Size: 24 366 384 762 85 1071 2973
Fragments arranged by size:
2973 1071 762 384 366 85 24
BsmI GAATG_Cn'
Cuts at: 3176 3269 3176
Size: 93 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2214 2265 2552 2580 2795 2927
Size: 399 400 24 51 287 28 215 132
Cuts at: 2927 2964 2984 3726 3811 4882 1391
Size: 37 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 400 399 287 215 132
85 51 37 28 24 20
BspEI T'CCGG_A
Cuts at: 5328 5328
Size: 5665
BspHI T'CATG_A
Cuts at: 3554 5284 3554
Size: 1730 3935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2130 3020 844
Size: 1286 890 3489
Fragments arranged by size:
3489 1286 890
BsrBI CCG'CTC
Cuts at: 1394 1823 2026 2359 2658 3552 1394
Size: 429 203 333 299 894 3507
Fragments arranged by size:
3507 894 429 333 299 203
BsrDI GCAATG_nn'
Cuts at: 65 1248 4160 4334 65
Size: 1183 2912 174 1396
Fragments arranged by size:
2912 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4309 4496 1700
Size: 110 2499 187 2869
Fragments arranged by size:
2869 2499 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5665
BssHII G'CGCG_C
Cuts at: 1925 2166 2248 2250 1925
Size: 241 82 2 5340
Fragments arranged by size:
5340 241 82 2
BssSI C'ACGA_G
Cuts at: 3725 4737 3725
Size: 1012 4653
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2304 2491 794
Size: 1510 187 3968
Fragments arranged by size:
3968 1510 187
BstBI TT'CG_AA
Cuts at: 2133 4469 2133
Size: 2336 3329
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5665
BstXI CCAn_nnnn'nTGG
Cuts at: 2770 2770
Size: 5665
BstYI r'GATC_y
Cuts at: 1972 2448 2995 3362 3747 3764 5216 5302 5314
Size: 476 547 367 385 17 1452 86 12
Cuts at: 5314 5660 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 547 476 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2202 2566 513
Size: 552 939 198 364 3612
Fragments arranged by size:
3612 939 552 364 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4926
Fragments arranged by size:
4926 683 56
BtsI GCAGTG_nn'
Cuts at: 2927 3181 3975 3995 2927
Size: 254 794 20 4597
Fragments arranged by size:
4597 794 254 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5630
DraI TTT'AAA
Cuts at: 2969 2969
Size: 5665
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4672 809
Size: 507 3356 1802
Fragments arranged by size:
3356 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3112 4001 5630
Size: 54 1425 50 275 18 1282 889 1629
Cuts at: 5630 8
Size: 43
Fragments arranged by size:
1629 1425 1282 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 1537
Size: 275 1300 4090
Fragments arranged by size:
4090 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 2723 3595 1353
Size: 825 545 872 3423
Fragments arranged by size:
3423 872 825 545
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2686 2834 4252 4626 4772 387
Size: 1064 138 1097 148 1418 374 146 1280
Fragments arranged by size:
1418 1280 1097 1064 374 148 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3728 4325 5112 5380 1239
Size: 393 2096 597 787 268 1524
Fragments arranged by size:
2096 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2479 2479
Size: 5665
EcoRI G'AATT_C
Cuts at: 2974 3352 2974
Size: 378 5287
FspI TGC'GCA
Cuts at: 2456 4171 2456
Size: 1715 3950
HaeII r_GCGC'y
Cuts at: 1678 2365 2407 2526 2664 2691 2708 2804 4812
Size: 687 42 119 138 27 17 96 2008
Cuts at: 4812 1678
Size: 2531
Fragments arranged by size:
2531 2008 687 138 119 96 42 27
17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2744 2776 4306
Size: 32 135 32 428 32 712 32 1530
Cuts at: 4306 4338 4380 4412 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1530 712 428 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3009 3255 669
Size: 988 281 1071 246 3079
Fragments arranged by size:
3079 1071 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5665
HpaI GTT'AAC
Cuts at: 3255 3255
Size: 5665
KasI G'GCGC_C
Cuts at: 2361 2522 2660 2687 2704 2800 2361
Size: 161 138 27 17 96 5226
Fragments arranged by size:
5226 161 138 96 27 17
KpnI G_GTAC'C
Cuts at: 2990 2990
Size: 5665
MfeI C'AATT_G
Cuts at: 3264 3264
Size: 5665
MluI A'CGCG_T
Cuts at: 2598 5541 2598
Size: 2943 2722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2619 2640 2649 4779 4963 1305
Size: 1314 21 9 2130 184 2007
Fragments arranged by size:
2130 2007 1314 184 21 9
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3843
Fragments arranged by size:
3843 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3623 3982 4141 518
Size: 648 2457 359 159 2042
Fragments arranged by size:
2457 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2204 2259 2280 2431 2554 2568 2586 2646
Size: 598 55 21 151 123 14 18 60
Cuts at: 2646 2886 3081 3758 4906 5151 1606
Size: 240 195 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 598 245 240 195 151
123 60 55 21 18 14
NarI GG'CG_CC
Cuts at: 2362 2523 2661 2688 2705 2801 2362
Size: 161 138 27 17 96 5226
Fragments arranged by size:
5226 161 138 96 27 17
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4174
Fragments arranged by size:
4174 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5665
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5665
NotI GC'GGCC_GC
Cuts at: 3112 3112
Size: 5665
NspI r_CATG'y
Cuts at: 1132 1229 2246 3023 4568 1132
Size: 97 1017 777 1545 2229
Fragments arranged by size:
2229 1545 1017 777 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5665
PciI A'CATG_T
Cuts at: 4564 4564
Size: 5665
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5665
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PmlI CAC'GTG
Cuts at: 2161 2231 2161
Size: 70 5595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 5247 5279 3734
Size: 32 1481 32 4120
Fragments arranged by size:
4120 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5665
PsiI TTA'TAA
Cuts at: 3235 3235
Size: 5665
PspOMI G'GGCC_C
Cuts at: 2548 2548
Size: 5665
PstI C_TGCA'G
Cuts at: 830 2487 3017 830
Size: 1657 530 3478
Fragments arranged by size:
3478 1657 530
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
SacII CC_GC'GG
Cuts at: 2205 2569 2205
Size: 364 5301
SalI G'TCGA_C
Cuts at: 1936 3007 1936
Size: 1071 4594
SapI GCTCTTCn'nnn_
Cuts at: 2178 2178
Size: 5665
SbfI CC_TGCA'GG
Cuts at: 2487 3017 2487
Size: 530 5135
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SexAI A'CCwGG_T
Cuts at: 2955 2955
Size: 5665
SfcI C'TryA_G
Cuts at: 826 1045 2483 2581 2641 3013 4148 4829 5020
Size: 219 1438 98 60 372 1135 681 191
Cuts at: 5020 826
Size: 1471
Fragments arranged by size:
1471 1438 1135 681 372 219 191 98
60
SfoI GGC'GCC
Cuts at: 2363 2524 2662 2689 2706 2802 2363
Size: 161 138 27 17 96 5226
Fragments arranged by size:
5226 161 138 96 27 17
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5665
SmaI CCC'GGG
Cuts at: 1872 2223 2562 2725 2992 1872
Size: 351 339 163 267 4545
Fragments arranged by size:
4545 351 339 267 163
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2399 3769 4670 4932 820
Size: 197 926 456 1370 901 262 1553
Fragments arranged by size:
1553 1370 926 901 456 262 197
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 3023 1132
Size: 1891 3774
SspI AAT'ATT
Cuts at: 5 52 3589 5
Size: 47 3537 2081
Fragments arranged by size:
3537 2081 47
StuI AGG'CCT
Cuts at: 1517 2387 1517
Size: 870 4795
StyI C'CwwG_G
Cuts at: 513 1065 2004 2439 3104 513
Size: 552 939 435 665 3074
Fragments arranged by size:
3074 939 665 552 435
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2912 3692 3709 3862 4047 4492 325
Size: 1255 1332 780 17 153 185 445 1498
Fragments arranged by size:
1498 1332 1255 780 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3911 5623 96
Size: 274 80 33 51 1443 1934 1712 138
Fragments arranged by size:
1934 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5625 5657 5625
Size: 32 5633
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5665
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5665
XmaI C'CCGG_G
Cuts at: 1870 2221 2560 2723 2990 1870
Size: 351 339 163 267 4545
Fragments arranged by size:
4545 351 339 267 163
XmnI GAAnn'nnTTC
Cuts at: 3794 3794
Size: 5665
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3473 276
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PpuMI PspXI PsrI PvuII RsrII SacI SanDI SfiI
SrfI SwaI XcmI