Restriction map for
pFN21AB7630
(Circular) (Six-base) MAPSORT of: pFN21AB7630.seq Check: 2513 from: 1 to: 5830
pFN21AB7630 5830 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3173 1937
Size: 1236 4594
Acc65I G'GTAC_C
Cuts at: 2896 3151 2896
Size: 255 5575
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2114 2476 3893 5277 5545 1632
Size: 482 362 1417 1384 268 1917
Fragments arranged by size:
1917 1417 1384 482 362 268
AflII C'TTAA_G
Cuts at: 820 1017 2324 820
Size: 197 1307 4326
Fragments arranged by size:
4326 1307 197
AflIII A'CryG_T
Cuts at: 1203 2762 4729 5706 1203
Size: 1559 1967 977 1327
Fragments arranged by size:
1967 1559 1327 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 2420 1166
Size: 1254 4576
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5830
AlwNI CAG_nnn'CTG
Cuts at: 3063 3573 5145 3063
Size: 510 1572 3748
Fragments arranged by size:
3748 1572 510
ApaI G_GGCC'C
Cuts at: 2027 2027
Size: 5830
ApaLI G'TGCA_C
Cuts at: 3887 5043 3887
Size: 1156 4674
ApoI r'AATT_y
Cuts at: 1493 3139 3365 3517 5626 1493
Size: 1646 226 152 2109 1697
Fragments arranged by size:
2109 1697 1646 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 2642 3155 1870
Size: 73 699 513 4545
Fragments arranged by size:
4545 699 513 73
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2896 3151 4606 618
Size: 325 217 627 1109 255 1455 1842
Fragments arranged by size:
1842 1455 1109 627 325 255 217
BanII G_rGCy'C
Cuts at: 1391 2027 2517 2649 2661 2760 2822 2867 2925
Size: 636 490 132 12 99 62 45 58
Cuts at: 2925 3149 1391
Size: 224 4072
Fragments arranged by size:
4072 636 490 224 132 99 62 58
45 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4859
BbvCI CC'TCA_GC
Cuts at: 2687 2687
Size: 5830
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4021 4055 1903
Size: 34 50 34 2000 34 3678
Fragments arranged by size:
3678 2000 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2049 3722 4932 2049
Size: 1673 1210 2947
Fragments arranged by size:
2947 1673 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5830
BfrBI ATG'CAT
Cuts at: 2171 2542 2171
Size: 371 5459
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4441 136
Size: 107 122 71 631 605 2769 1525
Fragments arranged by size:
2769 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5825 5825
Size: 5830
BlpI GC'TnA_GC
Cuts at: 3247 3247
Size: 5830
Bme1580I G_kGCm'C
Cuts at: 1790 2027 3891 5047 1790
Size: 237 1864 1156 2573
Fragments arranged by size:
2573 1864 1156 237
BmgBI CAC'GTC
Cuts at: 1318 2484 2765 3114 1318
Size: 1166 281 349 4034
Fragments arranged by size:
4034 1166 349 281
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2983 3056 3561
Size: 416 53 83 399 24 1566 73 505
Cuts at: 3561 4519 442
Size: 958 1753
Fragments arranged by size:
1753 1566 958 505 416 399 83 73
53 24
BmtI G_CTAG'C
Cuts at: 1056 2874 1056
Size: 1818 4012
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2246 2468 2872 2999 4490 1239
Size: 836 171 222 404 127 1491 2579
Fragments arranged by size:
2579 1491 836 404 222 171 127
Bpu10I CC'TnA_GC
Cuts at: 2687 2687
Size: 5830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2690 3955 4820 5118 2690
Size: 1265 865 298 3402
Fragments arranged by size:
3402 1265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4493 882
Size: 617 2994 2219
Fragments arranged by size:
2994 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3621 493
Size: 711 2417 2702
Fragments arranged by size:
2702 2417 711
BsaBI GATnn'nnATC
Cuts at: 5824 5824
Size: 5830
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3637 4019 4654 275
Size: 53 83 186 936 2104 382 635 1451
Fragments arranged by size:
2104 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4263 4661 4935 5082 5493 4263
Size: 398 274 147 411 4600
Fragments arranged by size:
4600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2423 2441 2453 2471 1178
Size: 30 165 30 1020 18 12 18 4537
Fragments arranged by size:
4537 1020 165 30 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 1186
Size: 973 4857
BseYI C'CCAG_C
Cuts at: 2394 2774 2866 5033 5529 2394
Size: 380 92 2167 496 2695
Fragments arranged by size:
2695 2167 496 380 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3027 1163
Size: 573 1291 3966
Fragments arranged by size:
3966 1291 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3280 4041 5069 1540
Size: 275 186 1279 761 1028 2301
Fragments arranged by size:
2301 1279 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2517 2661 2822 3891 3976 5047 2517
Size: 144 161 1069 85 1071 3300
Fragments arranged by size:
3300 1071 1069 161 144 85
BsmI GAATG_Cn'
Cuts at: 2171 2342 3341 3434 2171
Size: 171 999 93 4567
Fragments arranged by size:
4567 999 171 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2517 2649 2661 2760 2822 2867
Size: 399 237 490 132 12 99 62 45
Cuts at: 2867 2925 3149 3891 3976 5047 1391
Size: 58 224 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 490 399 237 224 132
99 85 62 58 45 12
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 3719 5449 3719
Size: 1730 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2720 3185 844
Size: 1876 465 3489
Fragments arranged by size:
3489 1876 465
BsrBI CCG'CTC
Cuts at: 1394 1823 3717 1394
Size: 429 1894 3507
Fragments arranged by size:
3507 1894 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4325 4499 65
Size: 1183 3077 174 1396
Fragments arranged by size:
3077 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2436 4474 4661 1700
Size: 110 626 2038 187 2869
Fragments arranged by size:
2869 2038 626 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 3890 4902 3890
Size: 1012 4818
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2750 794
Size: 1956 3874
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 2389 1184
Size: 1205 4625
BstXI CCAn_nnnn'nTGG
Cuts at: 2932 2932
Size: 5830
BstYI r'GATC_y
Cuts at: 1972 3160 3527 3912 3929 5381 5467 5479 5825
Size: 1188 367 385 17 1452 86 12 346
Cuts at: 5825 1972
Size: 1977
Fragments arranged by size:
1977 1452 1188 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5278
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5091
Fragments arranged by size:
5091 683 56
BtsI GCAGTG_nn'
Cuts at: 3346 4140 4160 3346
Size: 794 20 5016
Fragments arranged by size:
5016 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5795
DraI TTT'AAA
Cuts at: 3134 3134
Size: 5830
DraIII CAC_nnn'GTG
Cuts at: 2592 2592
Size: 5830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4837 809
Size: 507 3521 1802
Fragments arranged by size:
3521 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2254 3277 4166
Size: 54 1425 50 275 18 424 1023 889
Cuts at: 4166 5795 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1023 889 424 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3277 1537
Size: 275 1465 4090
Fragments arranged by size:
4090 1465 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2855 3760 1353
Size: 1502 905 3423
Fragments arranged by size:
3423 1502 905
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2031 2800 4417 4791 4937 387
Size: 1064 138 442 769 1617 374 146 1280
Fragments arranged by size:
1617 1280 1064 769 442 374 146 138
EcoICRI GAG'CTC
Cuts at: 2515 2659 2820 2515
Size: 144 161 5525
Fragments arranged by size:
5525 161 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2114 2246 2468 2476 2872 2999
Size: 393 443 39 132 222 8 396 127
Cuts at: 2999 3893 4490 5277 5545 1239
Size: 894 597 787 268 1524
Fragments arranged by size:
1524 894 787 597 443 396 393 268
222 132 127 39 8
EcoRI G'AATT_C
Cuts at: 3139 3517 3139
Size: 378 5452
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 3054 4977 1678
Size: 1376 1923 2531
Fragments arranged by size:
2531 1923 1376
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2502 2534 2551 2558 2583
Size: 32 135 32 930 32 17 7 25
Cuts at: 2583 2590 2648 2680 4471 4503 4545 4577 1373
Size: 7 58 32 1791 32 42 32 2626
Fragments arranged by size:
2626 1791 930 135 58 42 32 32
32 32 32 32 25 17 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2208 3174 3420 669
Size: 988 281 270 966 246 3079
Fragments arranged by size:
3079 988 966 281 270 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5830
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KpnI G_GTAC'C
Cuts at: 2900 3155 2900
Size: 255 5575
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2544 2650 4944 5128 1305
Size: 1239 106 2294 184 2007
Fragments arranged by size:
2294 2007 1239 184 106
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4008
Fragments arranged by size:
4008 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2420 2930 3788 4147 4306 518
Size: 648 1254 510 858 359 159 2042
Fragments arranged by size:
2042 1254 858 648 510 359 159
MspA1I CmG'CkG
Cuts at: 1606 3246 3923 5071 5316 1606
Size: 1640 677 1148 245 2120
Fragments arranged by size:
2120 1640 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5278
NdeI CA'TA_TG
Cuts at: 387 2083 387
Size: 1696 4134
NheI G'CTAG_C
Cuts at: 1052 2870 1052
Size: 1818 4012
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NsiI A_TGCA'T
Cuts at: 2173 2544 2173
Size: 371 5459
NspI r_CATG'y
Cuts at: 1132 1229 2546 3188 4733 1132
Size: 97 1317 642 1545 2229
Fragments arranged by size:
2229 1545 1317 642 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5830
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2066 2563 1108
Size: 958 497 4375
Fragments arranged by size:
4375 958 497
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 5412 5444 3899
Size: 32 1481 32 4285
Fragments arranged by size:
4285 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PspOMI G'GGCC_C
Cuts at: 2023 2023
Size: 5830
PspXI vC'TCGA_Gb
Cuts at: 2642 2642
Size: 5830
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2435 2467 2435
Size: 32 5798
PstI C_TGCA'G
Cuts at: 830 3182 830
Size: 2352 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
SacI G_AGCT'C
Cuts at: 2517 2661 2822 2517
Size: 144 161 5525
Fragments arranged by size:
5525 161 144
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SapI GCTCTTCn'nnn_
Cuts at: 2855 2855
Size: 5830
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SfcI C'TryA_G
Cuts at: 826 1045 2101 2680 3178 4313 4994 5185 826
Size: 219 1056 579 498 1135 681 191 1471
Fragments arranged by size:
1471 1135 1056 681 579 498 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 3157 1872
Size: 1285 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2324 2642 2705 3934 4835 5097
Size: 197 926 381 318 63 1229 901 262
Cuts at: 5097 820
Size: 1553
Fragments arranged by size:
1553 1229 926 901 381 318 262 197
63
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 2546 3188 1132
Size: 1414 642 3774
Fragments arranged by size:
3774 1414 642
SspI AAT'ATT
Cuts at: 5 52 2109 3754 5
Size: 47 2057 1645 2081
Fragments arranged by size:
2081 2057 1645 47
StuI AGG'CCT
Cuts at: 1517 3122 1517
Size: 1605 4225
StyI C'CwwG_G
Cuts at: 513 1065 2334 3269 513
Size: 552 1269 935 3074
Fragments arranged by size:
3074 1269 935 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2432 3002 3857 3874 4027 4212 4657
Size: 1255 852 570 855 17 153 185 445
Cuts at: 4657 325
Size: 1498
Fragments arranged by size:
1498 1255 855 852 570 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2313 4076 5788
Size: 274 80 33 51 1443 336 1763 1712
Cuts at: 5788 96
Size: 138
Fragments arranged by size:
1763 1712 1443 336 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2262 2294 5790 5822 2262
Size: 32 3496 32 2270
Fragments arranged by size:
3496 2270 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5830
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XhoI C'TCGA_G
Cuts at: 1943 2642 1943
Size: 699 5131
XmaI C'CCGG_G
Cuts at: 1870 3155 1870
Size: 1285 4545
XmnI GAAnn'nnTTC
Cuts at: 3959 3959
Size: 5830
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PvuII RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI