Restriction map for
pFN21AB7628
(Circular) (Six-base) MAPSORT of: pFN21AB7628.seq Check: 3331 from: 1 to: 5824
pFN21AB7628 5824 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2347 2347
Size: 5824
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3167 1937
Size: 1230 4594
Acc65I G'GTAC_C
Cuts at: 2068 3145 2068
Size: 1077 4747
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2523 3887 5271 5539 1632
Size: 891 1364 1384 268 1917
Fragments arranged by size:
1917 1384 1364 891 268
AfeI AGC'GCT
Cuts at: 2282 2282
Size: 5824
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 4723 5700 1203
Size: 3520 977 1327
Fragments arranged by size:
3520 1327 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 2552 4553 2552
Size: 2001 3823
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5824
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5824
AlwNI CAG_nnn'CTG
Cuts at: 3567 5139 3567
Size: 1572 4252
ApaLI G'TGCA_C
Cuts at: 3881 5037 3881
Size: 1156 4668
ApoI r'AATT_y
Cuts at: 1493 3133 3359 3511 5620 1493
Size: 1640 226 152 2109 1697
Fragments arranged by size:
2109 1697 1640 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2137 2747 3016 3149 1870
Size: 73 120 74 610 269 133 4545
Fragments arranged by size:
4545 610 269 133 120 74 73
BamHI G'GATC_C
Cuts at: 2753 3154 3521 2753
Size: 401 367 5056
Fragments arranged by size:
5056 401 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2068 2195 2495 3145 4600
Size: 325 217 627 281 127 300 650 1455
Cuts at: 4600 618
Size: 1842
Fragments arranged by size:
1842 1455 650 627 325 300 281 217
127
BanII G_rGCy'C
Cuts at: 1391 2861 3084 3143 1391
Size: 1470 223 59 4072
Fragments arranged by size:
4072 1470 223 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4853
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2308 2342 4015 4049 1903
Size: 34 50 34 287 34 1673 34 3678
Fragments arranged by size:
3678 1673 287 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BfrBI ATG'CAT
Cuts at: 2875 2875
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2778 4435 136
Size: 107 122 71 631 605 1106 1657 1525
Fragments arranged by size:
1657 1525 1106 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 3241 3241
Size: 5824
Bme1580I G_kGCm'C
Cuts at: 1790 2919 3885 5041 1790
Size: 1129 966 1156 2573
Fragments arranged by size:
2573 1156 1129 966
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5824
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2418 2857 2914
Size: 416 53 83 399 24 1001 439 57
Cuts at: 2914 3555 4513 442
Size: 641 958 1753
Fragments arranged by size:
1753 1001 958 641 439 416 399 83
57 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3042 3042
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4484 1239
Size: 3245 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2252 3949 4814 5112 2252
Size: 1697 865 298 2964
Fragments arranged by size:
2964 1697 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4487 882
Size: 617 2988 2219
Fragments arranged by size:
2988 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3615 493
Size: 711 2411 2702
Fragments arranged by size:
2702 2411 711
BsaBI GATnn'nnATC
Cuts at: 5818 5818
Size: 5824
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2183 3631 4013 4648
Size: 53 83 186 936 650 1448 382 635
Cuts at: 4648 275
Size: 1451
Fragments arranged by size:
1451 1448 936 650 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4257 4655 4929 5076 5487 4257
Size: 398 274 147 411 4594
Fragments arranged by size:
4594 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3085 3115 1178
Size: 30 165 30 1682 30 3887
Fragments arranged by size:
3887 1682 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2175 2402 3128 1186
Size: 989 227 726 3882
Fragments arranged by size:
3882 989 726 227
BseYI C'CCAG_C
Cuts at: 2984 3083 5027 5523 2984
Size: 99 1944 496 3285
Fragments arranged by size:
3285 1944 496 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2509 1163
Size: 573 773 4478
Fragments arranged by size:
4478 773 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2433 3274 4035 5063 1540
Size: 275 186 432 841 761 1028 2301
Fragments arranged by size:
2301 1028 841 761 432 275 186
BsiHKAI G_wGCw'C
Cuts at: 2861 3047 3885 3970 5041 2861
Size: 186 838 85 1071 3644
Fragments arranged by size:
3644 1071 838 186 85
BsmI GAATG_Cn'
Cuts at: 2021 2875 3335 3428 2021
Size: 854 460 93 4417
Fragments arranged by size:
4417 854 460 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2658 2658
Size: 5824
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2861 2919 3047 3084 3143 3885 3970
Size: 399 1071 58 128 37 59 742 85
Cuts at: 3970 5041 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1071 742 399 128 85 59
58 37
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 2478 3713 5443 2478
Size: 1235 1730 2859
Fragments arranged by size:
2859 1730 1235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2347 3179 844
Size: 1503 832 3489
Fragments arranged by size:
3489 1503 832
BsrBI CCG'CTC
Cuts at: 1394 1823 2266 3711 1394
Size: 429 443 1445 3507
Fragments arranged by size:
3507 1445 443 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2859 4319 4493 65
Size: 1183 1611 1460 174 1396
Fragments arranged by size:
1611 1460 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2512 4468 4655 1700
Size: 110 702 1956 187 2869
Fragments arranged by size:
2869 1956 702 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2316 794
Size: 1522 4302
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5824
BstXI CCAn_nnnn'nTGG
Cuts at: 2707 2707
Size: 5824
BstYI r'GATC_y
Cuts at: 1972 2753 3154 3521 3906 3923 5375 5461 5473
Size: 781 401 367 385 17 1452 86 12
Cuts at: 5473 5819 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 781 401 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2216 2248 2700 513
Size: 552 1151 32 452 3637
Fragments arranged by size:
3637 1151 552 452 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5085
Fragments arranged by size:
5085 683 56
BtsI GCAGTG_nn'
Cuts at: 2417 2497 2529 3060 3340 4134 4154 2417
Size: 80 32 531 280 794 20 4087
Fragments arranged by size:
4087 794 531 280 80 32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5789
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4831 809
Size: 507 3515 1802
Fragments arranged by size:
3515 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2349 2430
Size: 54 1425 50 275 18 297 222 81
Cuts at: 2430 2514 3271 4160 5789 8
Size: 84 757 889 1629 43
Fragments arranged by size:
1629 1425 889 757 297 275 222 84
81 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2430 3271 1537
Size: 275 618 841 4090
Fragments arranged by size:
4090 841 618 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3754 1353
Size: 2401 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4411 4785 4931 387
Size: 1064 138 2822 374 146 1280
Fragments arranged by size:
2822 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2859 2859
Size: 5824
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2523 3887 4484 5271 5539 1239
Size: 393 891 1364 597 787 268 1524
Fragments arranged by size:
1524 1364 891 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2779 2779
Size: 5824
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
FspI TGC'GCA
Cuts at: 2233 4330 2233
Size: 2097 3727
FspAI rTGC'GCAy
Cuts at: 2233 2233
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 2117 2145 2284 4971 1678
Size: 439 28 139 2687 2531
Fragments arranged by size:
2687 2531 439 139 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2538 2570 2930 2962 3059
Size: 32 135 32 966 32 360 32 97
Cuts at: 3059 3091 4465 4497 4539 4571 1373
Size: 32 1374 32 42 32 2626
Fragments arranged by size:
2626 1374 966 360 135 97 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3168 3414 669
Size: 988 281 1230 246 3079
Fragments arranged by size:
3079 1230 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 2072 3149 2072
Size: 1077 4747
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2025 2166 3057 4938 5122 1305
Size: 720 141 891 1881 184 2007
Fragments arranged by size:
2007 1881 891 720 184 141
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4002
Fragments arranged by size:
4002 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3782 4141 4300 518
Size: 648 2616 359 159 2042
Fragments arranged by size:
2616 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2133 2218 3240 3917 5065 5310 1606
Size: 527 85 1022 677 1148 245 2120
Fragments arranged by size:
2120 1148 1022 677 527 245 85
NaeI GCC'GGC
Cuts at: 2514 2514
Size: 5824
NcoI C'CATG_G
Cuts at: 513 1065 2700 513
Size: 552 1635 3637
Fragments arranged by size:
3637 1635 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NgoMIV G'CCGG_C
Cuts at: 2512 2512
Size: 5824
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NsiI A_TGCA'T
Cuts at: 2877 2877
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 2293 2594 3182 4727 1132
Size: 97 1064 301 588 1545 2229
Fragments arranged by size:
2229 1545 1064 588 301 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5824
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5824
PfoI T'CCnGG_A
Cuts at: 2559 2559
Size: 5824
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 5406 5438 3893
Size: 32 1481 32 4279
Fragments arranged by size:
4279 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PspXI vC'TCGA_Gb
Cuts at: 2137 2137
Size: 5824
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2237 2269 2237
Size: 32 5792
PstI C_TGCA'G
Cuts at: 830 2243 2536 3176 830
Size: 1413 293 640 3478
Fragments arranged by size:
3478 1413 640 293
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
SacI G_AGCT'C
Cuts at: 2861 2861
Size: 5824
SacII CC_GC'GG
Cuts at: 2219 2219
Size: 5824
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 2696 4072 5784 2696
Size: 1376 1712 2736
Fragments arranged by size:
2736 1712 1376
SexAI A'CCwGG_T
Cuts at: 2610 2610
Size: 5824
SfcI C'TryA_G
Cuts at: 826 1045 2239 2532 2632 3172 4307 4988 5179
Size: 219 1194 293 100 540 1135 681 191
Cuts at: 5179 826
Size: 1471
Fragments arranged by size:
1471 1194 1135 681 540 293 219 191
100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 2065 3151 1872
Size: 193 1086 4545
Fragments arranged by size:
4545 1086 193
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2137 2267 3016 3928 4829 5091
Size: 197 926 194 130 749 912 901 262
Cuts at: 5091 820
Size: 1553
Fragments arranged by size:
1553 926 912 901 749 262 197 194
130
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 3182 1132
Size: 2050 3774
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5824
StyI C'CwwG_G
Cuts at: 513 1065 2700 2706 3263 513
Size: 552 1635 6 557 3074
Fragments arranged by size:
3074 1635 557 552 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2183 3851 3868 4021 4206 4651 325
Size: 1255 603 1668 17 153 185 445 1498
Fragments arranged by size:
1668 1498 1255 603 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2694 2865 4070
Size: 274 80 33 51 1443 717 171 1205
Cuts at: 4070 5782 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1205 717 274 171 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2745 2777 2781 2813 3085 3117 5784 5816 2745
Size: 32 4 32 272 32 2667 32 2753
Fragments arranged by size:
2753 2667 272 32 32 32 32 4
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XhoI C'TCGA_G
Cuts at: 1943 2137 3016 1943
Size: 194 879 4751
Fragments arranged by size:
4751 879 194
XmaI C'CCGG_G
Cuts at: 1870 2063 3149 1870
Size: 193 1086 4545
Fragments arranged by size:
4545 1086 193
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI
PstI PvuI SacI SacII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
KasI NarI NruI PacI PmlI PpuMI PspOMI PvuII
RsrII SanDI SapI SfiI SfoI SrfI SwaI XcmI