Restriction map for
pFN21AB7626
(Circular) (Six-base) MAPSORT of: pFN21AB7626.seq Check: 1319 from: 1 to: 5821
pFN21AB7626 5821 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3056 3056
Size: 5821
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 1937
Size: 1227 4594
Acc65I G'GTAC_C
Cuts at: 2874 3142 2874
Size: 268 5553
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2819 2949 3884 5268 5536 1632
Size: 1187 130 935 1384 268 1917
Fragments arranged by size:
1917 1384 1187 935 268 130
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 2842 4652 2842
Size: 1810 4011
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3129 1184
Size: 1945 3876
AlwNI CAG_nnn'CTG
Cuts at: 2465 3564 5136 2465
Size: 1099 1572 3150
Fragments arranged by size:
3150 1572 1099
ApaI G_GGCC'C
Cuts at: 2662 2662
Size: 5821
ApaLI G'TGCA_C
Cuts at: 3878 5034 3878
Size: 1156 4665
ApoI r'AATT_y
Cuts at: 1493 3130 3356 3508 5617 1493
Size: 1637 226 152 2109 1697
Fragments arranged by size:
2109 1697 1637 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 3146 1870
Size: 73 1203 4545
Fragments arranged by size:
4545 1203 73
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2143 2201 2526 2556 2874
Size: 325 217 627 356 58 325 30 318
Cuts at: 2874 3142 4597 618
Size: 268 1455 1842
Fragments arranged by size:
1842 1455 627 356 325 325 318 268
217 58 30
BanII G_rGCy'C
Cuts at: 1391 2108 2662 3140 1391
Size: 717 554 478 4072
Fragments arranged by size:
4072 717 554 478
BbeI G_GCGC'C
Cuts at: 2147 2530 2560 2147
Size: 383 30 5408
Fragments arranged by size:
5408 383 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2250 928
Size: 971 351 4499
Fragments arranged by size:
4499 971 351
BbvCI CC'TCA_GC
Cuts at: 3117 3117
Size: 5821
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 2112 4432
Size: 107 122 71 631 605 406 34 2320
Cuts at: 4432 136
Size: 1525
Fragments arranged by size:
2320 1525 631 605 406 122 107 71
34
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 3238 3238
Size: 5821
Bme1580I G_kGCm'C
Cuts at: 1790 2204 2662 3882 5038 1790
Size: 414 458 1220 1156 2573
Fragments arranged by size:
2573 1220 1156 458 414
BmgBI CAC'GTC
Cuts at: 1318 2158 2540 1318
Size: 840 382 4599
Fragments arranged by size:
4599 840 382
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2677 3552 4510
Size: 416 53 83 399 24 1260 875 958
Cuts at: 4510 442
Size: 1753
Fragments arranged by size:
1753 1260 958 875 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4481 1239
Size: 3242 2579
Bpu10I CC'TnA_GC
Cuts at: 3117 3117
Size: 5821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3946 4811 5109 3946
Size: 865 298 4658
Fragments arranged by size:
4658 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4484 882
Size: 617 2985 2219
Fragments arranged by size:
2985 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2728 3612 493
Size: 711 1524 884 2702
Fragments arranged by size:
2702 1524 884 711
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2144 2527 2557 2995
Size: 53 83 186 936 611 383 30 438
Cuts at: 2995 3013 3628 4010 4645 275
Size: 18 615 382 635 1451
Fragments arranged by size:
1451 936 635 615 611 438 383 382
186 83 53 30 18
BsaWI w'CCGG_w
Cuts at: 2842 4254 4652 4926 5073 5484 2842
Size: 1412 398 274 147 411 3179
Fragments arranged by size:
3179 1412 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2409 2439 2481 2511 1178
Size: 30 165 30 1006 30 42 30 4488
Fragments arranged by size:
4488 1006 165 42 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2603 2606 2609 2883 1186
Size: 1417 3 3 274 4124
Fragments arranged by size:
4124 1417 274 3 3
BseYI C'CCAG_C
Cuts at: 2582 2911 3102 5024 5520 2582
Size: 329 191 1922 496 2883
Fragments arranged by size:
2883 1922 496 329 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2478 1163
Size: 573 742 4506
Fragments arranged by size:
4506 742 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2073 2211 3271 4032 5060 1540
Size: 275 186 72 138 1060 761 1028 2301
Fragments arranged by size:
2301 1060 1028 761 275 186 138 72
BsiHKAI G_wGCw'C
Cuts at: 2830 3882 3967 5038 2830
Size: 1052 85 1071 3613
Fragments arranged by size:
3613 1071 1052 85
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2204 2662 2830 3140 3882 3967
Size: 399 318 96 458 168 310 742 85
Cuts at: 3967 5038 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 458 399 318 310 168
96 85
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2914 3056 3176 844
Size: 2070 142 120 3489
Fragments arranged by size:
3489 2070 142 120
BsrBI CCG'CTC
Cuts at: 1394 1823 2607 3708 1394
Size: 429 784 1101 3507
Fragments arranged by size:
3507 1101 784 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4316 4490 65
Size: 1183 3068 174 1396
Fragments arranged by size:
3068 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2234 2700 2842 3009 4465 4652 1700
Size: 110 424 466 142 167 1456 187 2869
Fragments arranged by size:
2869 1456 466 424 187 167 142 110
BsrGI T'GTAC_A
Cuts at: 96 2356 96
Size: 2260 3561
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2465 794
Size: 1671 4150
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 3151 3518 3903 3920 5372 5458 5470 5816
Size: 1179 367 385 17 1452 86 12 346
Cuts at: 5816 1972
Size: 1977
Fragments arranged by size:
1977 1452 1179 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2076 2133 2196 2205 2387 2560 2983
Size: 552 1011 57 63 9 182 173 423
Cuts at: 2983 513
Size: 3351
Fragments arranged by size:
3351 1011 552 423 182 173 63 57
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5082
Fragments arranged by size:
5082 683 56
BtsI GCAGTG_nn'
Cuts at: 2410 2620 3118 3337 4131 4151 2410
Size: 210 498 219 794 20 4080
Fragments arranged by size:
4080 794 498 219 210 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4828 809
Size: 507 3512 1802
Fragments arranged by size:
3512 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2208 3268
Size: 54 1425 50 275 18 240 138 1060
Cuts at: 3268 4157 5786 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1060 889 275 240 138 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2070 2208 3268 1537
Size: 275 258 138 1060 4090
Fragments arranged by size:
4090 1060 275 258 138
EarI CTCTTCn'nnn_
Cuts at: 1353 3751 1353
Size: 2398 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4408 4782 4928 387
Size: 1064 138 2819 374 146 1280
Fragments arranged by size:
2819 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2819 2949 3884 4481 5268 5536 1239
Size: 393 1187 130 935 597 787 268 1524
Fragments arranged by size:
1524 1187 935 787 597 393 268 130
EcoNI CCTnn'n_nnAGG
Cuts at: 2051 2499 2051
Size: 448 5373
EcoO109I rG'GnC_Cy
Cuts at: 2089 2658 2888 2089
Size: 569 230 5022
Fragments arranged by size:
5022 569 230
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
EcoRV GAT'ATC
Cuts at: 2990 2990
Size: 5821
FspI TGC'GCA
Cuts at: 2402 4327 2402
Size: 1925 3896
HaeII r_GCGC'y
Cuts at: 1678 2147 2530 2560 4968 1678
Size: 469 383 30 2408 2531
Fragments arranged by size:
2531 2408 469 383 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2735 2767 4462 4494 4536
Size: 32 135 32 1163 32 1695 32 42
Cuts at: 4536 4568 1373
Size: 32 2626
Fragments arranged by size:
2626 1695 1163 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3165 3411 669
Size: 988 281 1227 246 3079
Fragments arranged by size:
3079 1227 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5821
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KasI G'GCGC_C
Cuts at: 2143 2526 2556 2143
Size: 383 30 5408
Fragments arranged by size:
5408 383 30
KpnI G_GTAC'C
Cuts at: 2878 3146 2878
Size: 268 5553
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2084 2882 2899 4935 5119 1305
Size: 779 798 17 2036 184 2007
Fragments arranged by size:
2036 2007 798 779 184 17
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3999
Fragments arranged by size:
3999 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3779 4138 4297 518
Size: 648 2613 359 159 2042
Fragments arranged by size:
2613 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2078 2118 2135 2207 2671 2707 3237 3914
Size: 472 40 17 72 464 36 530 677
Cuts at: 3914 5062 5307 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 530 472 464 245 72
40 36 17
NaeI GCC'GGC
Cuts at: 2236 2236
Size: 5821
NarI GG'CG_CC
Cuts at: 2144 2527 2557 2144
Size: 383 30 5408
Fragments arranged by size:
5408 383 30
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5269
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NgoMIV G'CCGG_C
Cuts at: 2234 2234
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 2070 2208 3268 2070
Size: 138 1060 4623
Fragments arranged by size:
4623 1060 138
NspI r_CATG'y
Cuts at: 1132 1229 3179 4724 1132
Size: 97 1950 1545 2229
Fragments arranged by size:
2229 1950 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2918 1108
Size: 1810 4011
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PmlI CAC'GTG
Cuts at: 2728 2728
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2089 2888 2089
Size: 799 5022
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 2324 3391 2324
Size: 1067 4754
PspOMI G'GGCC_C
Cuts at: 2658 2658
Size: 5821
PstI C_TGCA'G
Cuts at: 830 2521 3173 830
Size: 1691 652 3478
Fragments arranged by size:
3478 1691 652
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
PvuII CAG'CTG
Cuts at: 2671 2671
Size: 5821
SacII CC_GC'GG
Cuts at: 2079 2136 2208 2079
Size: 57 72 5692
Fragments arranged by size:
5692 72 57
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SfcI C'TryA_G
Cuts at: 826 1045 2424 2517 3169 4304 4985 5176 826
Size: 219 1379 93 652 1135 681 191 1471
Fragments arranged by size:
1471 1379 1135 681 652 219 191 93
SfoI GGC'GCC
Cuts at: 2145 2528 2558 2145
Size: 383 30 5408
Fragments arranged by size:
5408 383 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3925 4826 5088 820
Size: 197 926 1982 901 262 1553
Fragments arranged by size:
1982 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5821
StyI C'CwwG_G
Cuts at: 513 1065 2278 2870 3060 3260 513
Size: 552 1213 592 190 200 3074
Fragments arranged by size:
3074 1213 592 552 200 190
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3112 3116 3848 3865 4018 4203 4648
Size: 1255 1532 4 732 17 153 185 445
Cuts at: 4648 325
Size: 1498
Fragments arranged by size:
1532 1498 1255 732 445 185 153 17
4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2356 4067 5779
Size: 274 80 33 51 1443 379 1711 1712
Cuts at: 5779 96
Size: 138
Fragments arranged by size:
1712 1711 1443 379 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2481 2513 2944 2976 5781 5813 2481
Size: 32 431 32 2805 32 2489
Fragments arranged by size:
2805 2489 431 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2107 2107
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI
FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII
SacI SanDI SapI SexAI SfiI SrfI SwaI