Restriction map for
pFN21AB7625
(Circular) (Six-base) MAPSORT of: pFN21AB7625.seq Check: 6884 from: 1 to: 5653
pFN21AB7625 5653 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2996 1937
Size: 1059 4594
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2405 3716 5100 5368 1632
Size: 773 1311 1384 268 1917
Fragments arranged by size:
1917 1384 1311 773 268
AflII C'TTAA_G
Cuts at: 820 1017 2200 820
Size: 197 1183 4273
Fragments arranged by size:
4273 1183 197
AflIII A'CryG_T
Cuts at: 1203 4552 5529 1203
Size: 3349 977 1327
Fragments arranged by size:
3349 1327 977
AgeI A'CCGG_T
Cuts at: 2478 4484 2478
Size: 2006 3647
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 2898 1166
Size: 1732 3921
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 3396 4968 3396
Size: 1572 4081
ApaLI G'TGCA_C
Cuts at: 3710 4866 3710
Size: 1156 4497
ApoI r'AATT_y
Cuts at: 1493 2379 2528 2561 2948 2962 3188 3340 5449
Size: 886 149 33 387 14 226 152 2109
Cuts at: 5449 1493
Size: 1697
Fragments arranged by size:
2109 1697 886 387 226 152 149 33
14
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2978 1870
Size: 73 1035 4545
Fragments arranged by size:
4545 1035 73
BamHI G'GATC_C
Cuts at: 2044 2264 2983 3350 2044
Size: 220 719 367 4347
Fragments arranged by size:
4347 719 367 220
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2857 2974 4429 618
Size: 325 217 627 1070 117 1455 1842
Fragments arranged by size:
1842 1455 1070 627 325 217 117
BanII G_rGCy'C
Cuts at: 1391 2972 1391
Size: 1581 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2709 928
Size: 971 810 3872
Fragments arranged by size:
3872 971 810
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3844 3878 1903
Size: 34 1907 34 3678
Fragments arranged by size:
3678 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4264 136
Size: 107 122 71 631 605 2592 1525
Fragments arranged by size:
2592 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2860 3714 4870 1790
Size: 1070 854 1156 2573
Fragments arranged by size:
2573 1156 1070 854
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3384 4342 442
Size: 416 53 83 399 24 1967 958 1753
Fragments arranged by size:
1967 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4313 1239
Size: 3074 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3778 4643 4941 3778
Size: 865 298 4490
Fragments arranged by size:
4490 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4316 882
Size: 617 2817 2219
Fragments arranged by size:
2817 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3444 493
Size: 711 2240 2702
Fragments arranged by size:
2702 2240 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3460 3842 4477 275
Size: 53 83 186 936 1927 382 635 1451
Fragments arranged by size:
1927 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2047 2478 4086 4484 4758 4905 5316 2047
Size: 431 1608 398 274 147 411 2384
Fragments arranged by size:
2384 1608 431 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5428
Fragments arranged by size:
5428 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5653
BseYI C'CCAG_C
Cuts at: 2145 4856 5352 2145
Size: 2711 496 2446
Fragments arranged by size:
2711 2446 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2048 1163
Size: 573 312 4768
Fragments arranged by size:
4768 573 312
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2052 3103 3864 4892 1540
Size: 275 186 51 1051 761 1028 2301
Fragments arranged by size:
2301 1051 1028 761 275 186 51
BsiHKAI G_wGCw'C
Cuts at: 3714 3799 4870 3714
Size: 85 1071 4497
Fragments arranged by size:
4497 1071 85
BsmI GAATG_Cn'
Cuts at: 2251 3164 3257 2251
Size: 913 93 4647
Fragments arranged by size:
4647 913 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2860 2972 3714 3799 4870 1391
Size: 399 1070 112 742 85 1071 2174
Fragments arranged by size:
2174 1071 1070 742 399 112 85
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2475 3008 844
Size: 1631 533 3489
Fragments arranged by size:
3489 1631 533
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4148 4322 65
Size: 1183 2900 174 1396
Fragments arranged by size:
2900 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2478 4297 4484 1700
Size: 110 668 1819 187 2869
Fragments arranged by size:
2869 1819 668 187 110
BsrGI T'GTAC_A
Cuts at: 96 2811 96
Size: 2715 2938
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 2589 3713 4725 2589
Size: 1124 1012 3517
Fragments arranged by size:
3517 1124 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5653
BstXI CCAn_nnnn'nTGG
Cuts at: 2848 2900 2848
Size: 52 5601
BstYI r'GATC_y
Cuts at: 1972 2044 2194 2264 2983 3350 3735 3752 5204
Size: 72 150 70 719 367 385 17 1452
Cuts at: 5204 5290 5302 5648 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 719 385 367 346 150 86
72 70 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2697 2841 513
Size: 552 939 693 144 3325
Fragments arranged by size:
3325 939 693 552 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 2794 3169 3963 3983 2794
Size: 375 794 20 4464
Fragments arranged by size:
4464 794 375 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2560 2957 2560
Size: 397 5256
DraIII CAC_nnn'GTG
Cuts at: 2864 2864
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3100 3989 5618
Size: 54 1425 50 275 18 1270 889 1629
Cuts at: 5618 8
Size: 43
Fragments arranged by size:
1629 1425 1270 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3583 1353
Size: 2230 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4240 4614 4760 387
Size: 1064 138 435 2216 374 146 1280
Fragments arranged by size:
2216 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2405 3716 4313 5100 5368 1239
Size: 393 773 1311 597 787 268 1524
Fragments arranged by size:
1524 1311 787 773 597 393 268
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
EcoRV GAT'ATC
Cuts at: 2338 2338
Size: 5653
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 4800 1678
Size: 3122 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2507 2539 4294 4326 4368
Size: 32 135 32 935 32 1755 32 42
Cuts at: 4368 4400 1373
Size: 32 2626
Fragments arranged by size:
2626 1755 935 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3243 669
Size: 988 281 1059 246 3079
Fragments arranged by size:
3079 1059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2405 748
Size: 1657 3996
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2172 4767 4951 1305
Size: 867 2595 184 2007
Fragments arranged by size:
2595 2007 867 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3831
Fragments arranged by size:
3831 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2898 3611 3970 4129 518
Size: 648 1732 713 359 159 2042
Fragments arranged by size:
2042 1732 713 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2152 3069 3746 4894 5139 1606
Size: 546 917 677 1148 245 2120
Fragments arranged by size:
2120 1148 917 677 546 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2697 513
Size: 552 939 693 3469
Fragments arranged by size:
3469 939 693 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 3011 4556 1132
Size: 97 1782 1545 2229
Fragments arranged by size:
2229 1782 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5653
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PstI C_TGCA'G
Cuts at: 830 2472 3005 830
Size: 1642 533 3478
Fragments arranged by size:
3478 1642 533
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 2427 2468 2567 3001 4136 4817 5008
Size: 219 1382 41 99 434 1135 681 191
Cuts at: 5008 826
Size: 1471
Fragments arranged by size:
1471 1382 1135 681 434 219 191 99
41
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2200 3757 4658 4920 820
Size: 197 926 257 1557 901 262 1553
Fragments arranged by size:
1557 1553 926 901 262 257 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 2284 152
Size: 2132 3521
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2004 2574 2697 3092 513
Size: 552 939 570 123 395 3074
Fragments arranged by size:
3074 939 570 552 395 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3680 3697 3850 4035 4480 325
Size: 1255 2100 17 153 185 445 1498
Fragments arranged by size:
2100 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2811 3899 5611
Size: 274 80 33 51 1443 834 1088 1712
Cuts at: 5611 96
Size: 138
Fragments arranged by size:
1712 1443 1088 834 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5613 5645 5613
Size: 32 5621
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2093 2093
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 2626 3782 2626
Size: 1156 4497
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI