Restriction map for
pFN21AB7624
(Circular) (Six-base) MAPSORT of: pFN21AB7624.seq Check: 9571 from: 1 to: 5809
pFN21AB7624 5809 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3619 278
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3152 1937
Size: 1215 4594
Acc65I G'GTAC_C
Cuts at: 3130 3130
Size: 5809
AclI AA'CG_TT
Cuts at: 1563 3936 4309 1563
Size: 2373 373 3063
Fragments arranged by size:
3063 2373 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 2490 3872 5256 5524 1632
Size: 695 163 1382 1384 268 1917
Fragments arranged by size:
1917 1384 1382 695 268 163
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5612
AflIII A'CryG_T
Cuts at: 1203 2160 2578 3106 4708 5685 1203
Size: 957 418 528 1602 977 1327
Fragments arranged by size:
1602 1327 977 957 528 418
AgeI A'CCGG_T
Cuts at: 4640 4640
Size: 5809
AhdI GACnn_n'nnGTC
Cuts at: 4538 4538
Size: 5809
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2382 1184
Size: 1198 4611
AlwNI CAG_nnn'CTG
Cuts at: 3552 5124 3552
Size: 1572 4237
ApaLI G'TGCA_C
Cuts at: 3866 5022 3866
Size: 1156 4653
ApoI r'AATT_y
Cuts at: 1493 2146 2330 2457 2483 2749 3118 3344 3496
Size: 653 184 127 26 266 369 226 152
Cuts at: 3496 5605 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 653 369 266 226 184 152
127 26
AseI AT'TA_AT
Cuts at: 160 1032 4363 160
Size: 872 3331 1606
Fragments arranged by size:
3331 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
AvaI C'yCGr_G
Cuts at: 1870 1943 3134 1870
Size: 73 1191 4545
Fragments arranged by size:
4545 1191 73
BamHI G'GATC_C
Cuts at: 3139 3506 3139
Size: 367 5442
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3130 4585 618
Size: 325 217 627 1343 1455 1842
Fragments arranged by size:
1842 1455 1343 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2100 3128 1391
Size: 709 1028 4072
Fragments arranged by size:
4072 1028 709
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4838
BbvCI CC'TCA_GC
Cuts at: 2618 2618
Size: 5809
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4000 4034 1903
Size: 34 2063 34 3678
Fragments arranged by size:
3678 2063 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3701 4911 3701
Size: 1210 4599
BclI T'GATC_A
Cuts at: 1548 2557 1548
Size: 1009 4800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4420 136
Size: 107 122 71 631 605 2748 1525
Fragments arranged by size:
2748 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5804 5804
Size: 5809
BlpI GC'TnA_GC
Cuts at: 2048 3226 2048
Size: 1178 4631
Bme1580I G_kGCm'C
Cuts at: 1790 3870 5026 1790
Size: 2080 1156 2573
Fragments arranged by size:
2573 2080 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5809
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3540 4498 442
Size: 416 53 83 399 24 2123 958 1753
Fragments arranged by size:
2123 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2122 4469 1239
Size: 883 2347 2579
Fragments arranged by size:
2579 2347 883
Bpu10I CC'TnA_GC
Cuts at: 2618 2618
Size: 5809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3934 4799 5097 3934
Size: 865 298 4646
Fragments arranged by size:
4646 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4472 882
Size: 617 2973 2219
Fragments arranged by size:
2973 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3600 493
Size: 711 2396 2702
Fragments arranged by size:
2702 2396 711
BsaBI GATnn'nnATC
Cuts at: 2562 5803 2562
Size: 3241 2568
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3616 3998 4633 275
Size: 53 83 186 936 2083 382 635 1451
Fragments arranged by size:
2083 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4242 4640 4914 5061 5472 4242
Size: 398 274 147 411 4579
Fragments arranged by size:
4579 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5584
Fragments arranged by size:
5584 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2004 3112 1186
Size: 818 1108 3883
Fragments arranged by size:
3883 1108 818
BseYI C'CCAG_C
Cuts at: 5012 5508 5012
Size: 496 5313
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3008 1163
Size: 573 1272 3964
Fragments arranged by size:
3964 1272 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3259 4020 5048 1540
Size: 275 186 1258 761 1028 2301
Fragments arranged by size:
2301 1258 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 3870 3955 5026 2100
Size: 1770 85 1071 2883
Fragments arranged by size:
2883 1770 1071 85
BsmI GAATG_Cn'
Cuts at: 3320 3413 3320
Size: 93 5716
BsmBI CGTCTCn'nnnn_
Cuts at: 2057 2057
Size: 5809
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 3128 3870 3955 5026 1391
Size: 399 310 1028 742 85 1071 2174
Fragments arranged by size:
2174 1071 1028 742 399 310 85
BspEI T'CCGG_A
Cuts at: 5472 5472
Size: 5809
BspHI T'CATG_A
Cuts at: 2554 3698 5428 2554
Size: 1144 1730 2935
Fragments arranged by size:
2935 1730 1144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3164 844
Size: 2320 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3696 1394
Size: 429 1873 3507
Fragments arranged by size:
3507 1873 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2427 2989 4304 4478 65
Size: 1183 1179 562 1315 174 1396
Fragments arranged by size:
1396 1315 1183 1179 562 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4453 4640 1700
Size: 110 2643 187 2869
Fragments arranged by size:
2869 2643 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5809
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5809
BssSI C'ACGA_G
Cuts at: 3869 4881 3869
Size: 1012 4797
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5809
BstBI TT'CG_AA
Cuts at: 4613 4613
Size: 5809
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5809
BstYI r'GATC_y
Cuts at: 1972 3139 3506 3891 3908 5360 5446 5458 5804
Size: 1167 367 385 17 1452 86 12 346
Cuts at: 5804 1972
Size: 1977
Fragments arranged by size:
1977 1452 1167 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4318
Fragments arranged by size:
4318 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5070
Fragments arranged by size:
5070 683 56
BtsI GCAGTG_nn'
Cuts at: 2684 3081 3325 4119 4139 2684
Size: 397 244 794 20 4354
Fragments arranged by size:
4354 794 397 244 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5809
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5774
DraI TTT'AAA
Cuts at: 2328 3113 2328
Size: 785 5024
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4816 809
Size: 507 3500 1802
Fragments arranged by size:
3500 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3256 4145 5774
Size: 54 1425 50 275 18 1426 889 1629
Cuts at: 5774 8
Size: 43
Fragments arranged by size:
1629 1426 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3256 1537
Size: 275 1444 4090
Fragments arranged by size:
4090 1444 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2875 3045 3089 3739 1353
Size: 1522 170 44 650 3423
Fragments arranged by size:
3423 1522 650 170 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4396 4770 4916 387
Size: 1064 138 2807 374 146 1280
Fragments arranged by size:
2807 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2098 2098
Size: 5809
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2122 2327 2490 3872 4469 5256 5524
Size: 393 490 205 163 1382 597 787 268
Cuts at: 5524 1239
Size: 1524
Fragments arranged by size:
1524 1382 787 597 490 393 268 205
163
EcoO109I rG'GnC_Cy
Cuts at: 2491 2491
Size: 5809
EcoRI G'AATT_C
Cuts at: 3118 3496 3118
Size: 378 5431
FspI TGC'GCA
Cuts at: 4315 4315
Size: 5809
HaeII r_GCGC'y
Cuts at: 1678 2045 4956 1678
Size: 367 2911 2531
Fragments arranged by size:
2911 2531 367
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2873 2905 4450 4482 4524
Size: 32 135 32 1301 32 1545 32 42
Cuts at: 4524 4556 1373
Size: 32 2626
Fragments arranged by size:
2626 1545 1301 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2279 3153 3399 669
Size: 988 281 341 874 246 3079
Fragments arranged by size:
3079 988 874 341 281 246
HindIII A'AGCT_T
Cuts at: 748 2175 748
Size: 1427 4382
HpaI GTT'AAC
Cuts at: 3399 3399
Size: 5809
KpnI G_GTAC'C
Cuts at: 3134 3134
Size: 5809
MfeI C'AATT_G
Cuts at: 3408 3408
Size: 5809
MluI A'CGCG_T
Cuts at: 5685 5685
Size: 5809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4923 5107 1305
Size: 3618 184 2007
Fragments arranged by size:
3618 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3987
Fragments arranged by size:
3987 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2364 3767 4126 4285 518
Size: 648 1198 1403 359 159 2042
Fragments arranged by size:
2042 1403 1198 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3225 3902 5050 5295 1606
Size: 1619 677 1148 245 2120
Fragments arranged by size:
2120 1619 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4318
Fragments arranged by size:
4318 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5809
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5809
NotI GC'GGCC_GC
Cuts at: 3256 3256
Size: 5809
NspI r_CATG'y
Cuts at: 1132 1229 2164 2582 3167 4712 1132
Size: 97 935 418 585 1545 2229
Fragments arranged by size:
2229 1545 935 585 418 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5809
PciI A'CATG_T
Cuts at: 2160 2578 4708 2160
Size: 418 2130 3261
Fragments arranged by size:
3261 2130 418
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5809
PfoI T'CCnGG_A
Cuts at: 2058 2058
Size: 5809
PmeI GTTT'AAAC
Cuts at: 3113 3113
Size: 5809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 5391 5423 3878
Size: 32 1481 32 4264
Fragments arranged by size:
4264 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2491 2491
Size: 5809
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5809
PsiI TTA'TAA
Cuts at: 3379 3379
Size: 5809
PstI C_TGCA'G
Cuts at: 830 2028 3161 830
Size: 1198 1133 3478
Fragments arranged by size:
3478 1198 1133
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5809
SacI G_AGCT'C
Cuts at: 2100 2100
Size: 5809
SalI G'TCGA_C
Cuts at: 1936 3151 1936
Size: 1215 4594
SbfI CC_TGCA'GG
Cuts at: 3161 3161
Size: 5809
ScaI AGT'ACT
Cuts at: 4057 5769 4057
Size: 1712 4097
SfcI C'TryA_G
Cuts at: 826 1045 2024 3157 4292 4973 5164 826
Size: 219 979 1133 1135 681 191 1471
Fragments arranged by size:
1471 1135 1133 979 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5809
SmaI CCC'GGG
Cuts at: 1872 3136 1872
Size: 1264 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3913 4814 5076 820
Size: 197 926 1970 901 262 1553
Fragments arranged by size:
1970 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3600 493
Size: 3107 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5809
SphI G_CATG'C
Cuts at: 1132 3167 1132
Size: 2035 3774
SspI AAT'ATT
Cuts at: 5 52 2650 3733 5
Size: 47 2598 1083 2081
Fragments arranged by size:
2598 2081 1083 47
StuI AGG'CCT
Cuts at: 1517 2810 1517
Size: 1293 4516
StyI C'CwwG_G
Cuts at: 513 1065 2004 3248 513
Size: 552 939 1244 3074
Fragments arranged by size:
3074 1244 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2045 3836 3853 4006 4191 4636 325
Size: 1255 465 1791 17 153 185 445 1498
Fragments arranged by size:
1791 1498 1255 465 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4055 5767 96
Size: 274 80 33 51 1443 2078 1712 138
Fragments arranged by size:
2078 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5769 5801 5769
Size: 32 5777
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5809
XbaI T'CTAG_A
Cuts at: 3145 3145
Size: 5809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2228 2228
Size: 5809
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5809
XmaI C'CCGG_G
Cuts at: 1870 3134 1870
Size: 1264 4545
XmnI GAAnn'nnTTC
Cuts at: 3938 3938
Size: 5809
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3617 276
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI