Restriction map for
pFN21AB7621
(Circular) (Six-base) MAPSORT of: pFN21AB7621.seq Check: 2216 from: 1 to: 5644
pFN21AB7621 5644 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3707 5091 5359 1632
Size: 2075 1384 268 1917
Fragments arranged by size:
2075 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 4543 5520 1203
Size: 3340 977 1327
Fragments arranged by size:
3340 1327 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5644
AlwNI CAG_nnn'CTG
Cuts at: 2599 2882 3387 4959 2599
Size: 283 505 1572 3284
Fragments arranged by size:
3284 1572 505 283
ApaI G_GGCC'C
Cuts at: 2445 2445
Size: 5644
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2953 3179 3331 5440 1493
Size: 1460 226 152 2109 1697
Fragments arranged by size:
2109 1697 1460 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2185 2969 1870
Size: 73 242 784 4545
Fragments arranged by size:
4545 784 242 73
BamHI G'GATC_C
Cuts at: 2974 3341 2974
Size: 367 5277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2083 2213 2255 2965 4420
Size: 325 217 627 296 130 42 710 1455
Cuts at: 4420 618
Size: 1842
Fragments arranged by size:
1842 1455 710 627 325 296 217 130
42
BanII G_rGCy'C
Cuts at: 1391 2445 2679 2847 2963 1391
Size: 1054 234 168 116 4072
Fragments arranged by size:
4072 1054 234 168 116
BbeI G_GCGC'C
Cuts at: 2087 2217 2259 2087
Size: 130 42 5472
Fragments arranged by size:
5472 130 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2853 928
Size: 971 954 3719
Fragments arranged by size:
3719 971 954
BbvCI CC'TCA_GC
Cuts at: 2814 2814
Size: 5644
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3835 3869 1903
Size: 34 1898 34 3678
Fragments arranged by size:
3678 1898 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2067 2197 4255
Size: 107 122 71 631 605 395 130 2058
Cuts at: 4255 136
Size: 1525
Fragments arranged by size:
2058 1525 631 605 395 130 122 107
71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 2445 3705 4861 1790
Size: 655 1260 1156 2573
Fragments arranged by size:
2573 1260 1156 655
BmgBI CAC'GTC
Cuts at: 1318 2035 1318
Size: 717 4927
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2922 3375 4333
Size: 416 53 83 399 24 1505 453 958
Cuts at: 4333 442
Size: 1753
Fragments arranged by size:
1753 1505 958 453 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2663 1056
Size: 1607 4037
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2621 2627 4304 1239
Size: 1382 6 1677 2579
Fragments arranged by size:
2579 1677 1382 6
Bpu10I CC'TnA_GC
Cuts at: 2814 2814
Size: 5644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2869 3769 4634 4932 2869
Size: 900 865 298 3581
Fragments arranged by size:
3581 900 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4307 882
Size: 617 2808 2219
Fragments arranged by size:
2808 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2011 2137 3435 493
Size: 711 807 126 1298 2702
Fragments arranged by size:
2702 1298 807 711 126
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2084 2214 2256 3451
Size: 53 83 186 936 551 130 42 1195
Cuts at: 3451 3833 4468 275
Size: 382 635 1451
Fragments arranged by size:
1451 1195 936 635 551 382 186 130
83 53 42
BsaWI w'CCGG_w
Cuts at: 2643 2854 4077 4475 4749 4896 5307 2643
Size: 211 1223 398 274 147 411 2980
Fragments arranged by size:
2980 1223 411 398 274 211 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2036 2066 2285 2315 2600
Size: 30 165 30 633 30 219 30 285
Cuts at: 2600 2630 1178
Size: 30 4192
Fragments arranged by size:
4192 633 285 219 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5644
BseYI C'CCAG_C
Cuts at: 2671 2808 4847 5343 2671
Size: 137 2039 496 2972
Fragments arranged by size:
2972 2039 496 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5071
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2145 2265 2310 2385 3094 3855
Size: 275 186 144 120 45 75 709 761
Cuts at: 3855 4883 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 709 275 186 144 120
75 45
BsiHKAI G_wGCw'C
Cuts at: 3705 3790 4861 3705
Size: 85 1071 4488
Fragments arranged by size:
4488 1071 85
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2445 2679 2847 2963 3705 3790 4861
Size: 399 655 234 168 116 742 85 1071
Cuts at: 4861 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 655 399 234 168 116
85
BspEI T'CCGG_A
Cuts at: 2643 5307 2643
Size: 2664 2980
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2680 2999 844
Size: 1836 319 3489
Fragments arranged by size:
3489 1836 319
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4139 4313 65
Size: 1183 2891 174 1396
Fragments arranged by size:
2891 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2200 2518 4288 4475 1700
Size: 110 390 318 1770 187 2869
Fragments arranged by size:
2869 1770 390 318 187 110
BsrGI T'GTAC_A
Cuts at: 96 2580 96
Size: 2484 3160
BssHII G'CGCG_C
Cuts at: 1925 2175 1925
Size: 250 5394
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 2129 1184
Size: 945 4699
BstXI CCAn_nnnn'nTGG
Cuts at: 2278 2278
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2974 3341 3726 3743 5195 5281 5293 5639
Size: 1002 367 385 17 1452 86 12 346
Cuts at: 5639 1972
Size: 1977
Fragments arranged by size:
1977 1452 1002 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2259 2277 2316 513
Size: 552 1194 18 39 3841
Fragments arranged by size:
3841 1194 552 39 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 3160 3954 3974 3160
Size: 794 20 4830
Fragments arranged by size:
4830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 2198 2262
Size: 54 1425 50 275 18 312 56 64
Cuts at: 2262 2307 2382 2482 3091 3980 5609 8
Size: 45 75 100 609 889 1629 43
Fragments arranged by size:
1629 1425 889 609 312 275 100 75
64 56 54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2142 2262 2307 2382 3091 1537
Size: 275 330 120 45 75 709 4090
Fragments arranged by size:
4090 709 330 275 120 75 45
EarI CTCTTCn'nnn_
Cuts at: 1353 3574 1353
Size: 2221 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2506 2778 4231 4605 4751 387
Size: 1064 138 917 272 1453 374 146 1280
Fragments arranged by size:
1453 1280 1064 917 374 272 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2621 2627 3707 4304 5091 5359 1239
Size: 393 989 6 1080 597 787 268 1524
Fragments arranged by size:
1524 1080 989 787 597 393 268 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2027 2817 2027
Size: 790 4854
EcoO109I rG'GnC_Cy
Cuts at: 2822 2822
Size: 5644
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2087 2217 2259 4791 1678
Size: 409 130 42 2532 2531
Fragments arranged by size:
2532 2531 409 130 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2456 2488 4285 4317 4359
Size: 32 135 32 884 32 1797 32 42
Cuts at: 4359 4391 1373
Size: 32 2626
Fragments arranged by size:
2626 1797 884 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2988 3234 669
Size: 988 281 1050 246 3079
Fragments arranged by size:
3079 1050 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KasI G'GCGC_C
Cuts at: 2083 2213 2255 2083
Size: 130 42 5472
Fragments arranged by size:
5472 130 42
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4758 4942 1305
Size: 3453 184 2007
Fragments arranged by size:
3453 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3822
Fragments arranged by size:
3822 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3602 3961 4120 518
Size: 648 843 1593 359 159 2042
Fragments arranged by size:
2042 1593 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2150 2156 2261 2456 2470 2488 2596 3060
Size: 544 6 105 195 14 18 108 464
Cuts at: 3060 3737 4885 5130 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 544 464 245 195 108
105 18 14 6
NaeI GCC'GGC
Cuts at: 2202 2520 2202
Size: 318 5326
NarI GG'CG_CC
Cuts at: 2084 2214 2256 2084
Size: 130 42 5472
Fragments arranged by size:
5472 130 42
NcoI C'CATG_G
Cuts at: 513 1065 2316 513
Size: 552 1251 3841
Fragments arranged by size:
3841 1251 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NgoMIV G'CCGG_C
Cuts at: 2200 2518 2200
Size: 318 5326
NheI G'CTAG_C
Cuts at: 1052 2659 1052
Size: 1607 4037
NotI GC'GGCC_GC
Cuts at: 2142 2262 3091 2142
Size: 120 829 4695
Fragments arranged by size:
4695 829 120
NspI r_CATG'y
Cuts at: 1132 1229 2044 3002 4547 1132
Size: 97 815 958 1545 2229
Fragments arranged by size:
2229 1545 958 815 97
PasI CC'CwG_GG
Cuts at: 1348 2894 1348
Size: 1546 4098
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2599 1108
Size: 1491 4153
PfoI T'CCnGG_A
Cuts at: 2022 2223 2022
Size: 201 5443
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PmlI CAC'GTG
Cuts at: 2137 2137
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2822 2822
Size: 5644
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PspOMI G'GGCC_C
Cuts at: 2441 2441
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2155 2996 830
Size: 1325 841 3478
Fragments arranged by size:
3478 1325 841
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
PvuII CAG'CTG
Cuts at: 2150 2470 2150
Size: 320 5324
SacII CC_GC'GG
Cuts at: 2262 2262
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SfcI C'TryA_G
Cuts at: 826 1045 2151 2323 2623 2992 4127 4808 4999
Size: 219 1106 172 300 369 1135 681 191
Cuts at: 4999 826
Size: 1471
Fragments arranged by size:
1471 1135 1106 681 369 300 219 191
172
SfiI GGCCn_nnn'nGGCC
Cuts at: 2197 2197
Size: 5644
SfoI GGC'GCC
Cuts at: 2085 2215 2257 2085
Size: 130 42 5472
Fragments arranged by size:
5472 130 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2187 2971 1872
Size: 315 784 4545
Fragments arranged by size:
4545 784 315
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2848 3748 4649 4911 820
Size: 197 926 905 900 901 262 1553
Fragments arranged by size:
1553 926 905 901 900 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2316 3083 513
Size: 552 1251 767 3074
Fragments arranged by size:
3074 1251 767 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2954 3671 3688 3841 4026 4471 325
Size: 1255 1374 717 17 153 185 445 1498
Fragments arranged by size:
1498 1374 1255 717 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2580 3890 5602
Size: 274 80 33 51 1443 603 1310 1712
Cuts at: 5602 96
Size: 138
Fragments arranged by size:
1712 1443 1310 603 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2185 2969 1870
Size: 315 784 4545
Fragments arranged by size:
4545 784 315
XmnI GAAnn'nnTTC
Cuts at: 3773 3773
Size: 5644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PspXI PsrI RsrII
SacI SanDI SapI SexAI SrfI SwaI XcmI