Restriction map for
pFN21AB7618
(Circular) (Six-base) MAPSORT of: pFN21AB7618.seq Check: 8437 from: 1 to: 5791
pFN21AB7618 5791 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3601 278
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3134 1937
Size: 1197 4594
Acc65I G'GTAC_C
Cuts at: 2610 3112 2610
Size: 502 5289
AclI AA'CG_TT
Cuts at: 1563 3918 4291 1563
Size: 2355 373 3063
Fragments arranged by size:
3063 2355 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2912 3854 5238 5506 1632
Size: 1280 942 1384 268 1917
Fragments arranged by size:
1917 1384 1280 942 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5594
AflIII A'CryG_T
Cuts at: 1203 2841 4690 5667 1203
Size: 1638 1849 977 1327
Fragments arranged by size:
1849 1638 1327 977
AgeI A'CCGG_T
Cuts at: 2506 4622 2506
Size: 2116 3675
AhdI GACnn_n'nnGTC
Cuts at: 4520 4520
Size: 5791
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5791
AlwNI CAG_nnn'CTG
Cuts at: 2573 3534 5106 2573
Size: 961 1572 3258
Fragments arranged by size:
3258 1572 961
ApaI G_GGCC'C
Cuts at: 2060 2126 2258 2456 2523 2060
Size: 66 132 198 67 5328
Fragments arranged by size:
5328 198 132 67 66
ApaLI G'TGCA_C
Cuts at: 3848 5004 3848
Size: 1156 4635
ApoI r'AATT_y
Cuts at: 1493 3100 3326 3478 5587 1493
Size: 1607 226 152 2109 1697
Fragments arranged by size:
2109 1697 1607 226 152
AscI GG'CGCG_CC
Cuts at: 2271 2271
Size: 5791
AseI AT'TA_AT
Cuts at: 160 1032 4345 160
Size: 872 3313 1606
Fragments arranged by size:
3313 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
AvaI C'yCGr_G
Cuts at: 1870 1943 2125 2180 2251 2333 2362 2401 2430
Size: 73 182 55 71 82 29 39 29
Cuts at: 2430 2480 2515 2536 2726 2747 2854 3116 1870
Size: 50 35 21 190 21 107 262 4545
Fragments arranged by size:
4545 262 190 182 107 82 73 71
55 50 39 35 29 29 21 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2495 2528 2495
Size: 33 5758
BamHI G'GATC_C
Cuts at: 2764 3121 3488 2764
Size: 357 367 5067
Fragments arranged by size:
5067 367 357
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2293 2525 2585 2610 2629
Size: 325 217 627 506 232 60 25 19
Cuts at: 2629 3112 4567 618
Size: 483 1455 1842
Fragments arranged by size:
1842 1455 627 506 483 325 232 217
60 25 19
BanII G_rGCy'C
Cuts at: 1391 2060 2126 2258 2265 2431 2437 2456 2487
Size: 669 66 132 7 166 6 19 31
Cuts at: 2487 2523 2670 2885 3110 1391
Size: 36 147 215 225 4072
Fragments arranged by size:
4072 669 225 215 166 147 132 66
36 31 19 7 6
BbeI G_GCGC'C
Cuts at: 2297 2529 2589 2633 2297
Size: 232 60 44 5455
Fragments arranged by size:
5455 232 60 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4820
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2728 2762 3982 4016 1903
Size: 34 791 34 1220 34 3678
Fragments arranged by size:
3678 1220 791 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3683 4893 3683
Size: 1210 4581
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2312 4402 136
Size: 107 122 71 631 605 640 2090 1525
Fragments arranged by size:
2090 1525 640 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5786 5786
Size: 5791
BlpI GC'TnA_GC
Cuts at: 3208 3208
Size: 5791
Bme1580I G_kGCm'C
Cuts at: 1790 2060 2126 2258 2456 2523 2649 2741 3852
Size: 270 66 132 198 67 126 92 1111
Cuts at: 3852 5008 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1111 270 198 132 126 92
67 66
BmgBI CAC'GTC
Cuts at: 1318 2697 1318
Size: 1379 4412
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3522 4480 442
Size: 416 53 83 399 24 2105 958 1753
Fragments arranged by size:
2105 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5791
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 5791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2451 2654 2977 4451 1239
Size: 1212 203 323 1474 2579
Fragments arranged by size:
2579 1474 1212 323 203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2072 3916 4781 5079 2072
Size: 1844 865 298 2784
Fragments arranged by size:
2784 1844 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2012 2777 4454 882
Size: 617 513 765 1677 2219
Fragments arranged by size:
2219 1677 765 617 513
BsaAI yAC'GTr
Cuts at: 493 1204 2161 2844 2940 3041 3582 493
Size: 711 957 683 96 101 541 2702
Fragments arranged by size:
2702 957 711 683 541 101 96
BsaBI GATnn'nnATC
Cuts at: 5785 5785
Size: 5791
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2214 2294 2442 2526
Size: 53 83 186 936 681 80 148 84
Cuts at: 2526 2579 2586 2630 3598 3980 4615 275
Size: 53 7 44 968 382 635 1451
Fragments arranged by size:
1451 968 936 681 635 382 186 148
84 83 80 53 53 44 7
BsaWI w'CCGG_w
Cuts at: 2506 2994 4224 4622 4896 5043 5454 2506
Size: 488 1230 398 274 147 411 2843
Fragments arranged by size:
2843 1230 488 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5566
Fragments arranged by size:
5566 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2225 2228 2524 1186
Size: 1039 3 296 4453
Fragments arranged by size:
4453 1039 296 3
BseYI C'CCAG_C
Cuts at: 2297 2375 2455 4994 5490 2297
Size: 78 80 2539 496 2598
Fragments arranged by size:
2598 2539 496 80 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2920 1163
Size: 573 1184 4034
Fragments arranged by size:
4034 1184 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3241 4002 5030 1540
Size: 275 186 1240 761 1028 2301
Fragments arranged by size:
2301 1240 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2885 3852 3937 5008 2885
Size: 967 85 1071 3668
Fragments arranged by size:
3668 1071 967 85
BsmI GAATG_Cn'
Cuts at: 3302 3395 3302
Size: 93 5698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2126 2258 2265 2431 2437 2456
Size: 399 270 66 132 7 166 6 19
Cuts at: 2456 2487 2523 2649 2670 2741 2885 3110 3852
Size: 31 36 126 21 71 144 225 742
Cuts at: 3852 3937 5008 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 270 225 166 144
132 126 85 71 66 36 31 21
19 7 6
BspEI T'CCGG_A
Cuts at: 5454 5454
Size: 5791
BspHI T'CATG_A
Cuts at: 3680 5410 3680
Size: 1730 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2291 3146 844
Size: 1447 855 3489
Fragments arranged by size:
3489 1447 855
BsrBI CCG'CTC
Cuts at: 1394 1823 3678 1394
Size: 429 1855 3507
Fragments arranged by size:
3507 1855 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4286 4460 65
Size: 1183 3038 174 1396
Fragments arranged by size:
3038 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2506 2528 4435 4622 1700
Size: 110 696 22 1907 187 2869
Fragments arranged by size:
2869 1907 696 187 110 22
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5791
BssHII G'CGCG_C
Cuts at: 1925 2271 2819 2847 2966 1925
Size: 346 548 28 119 4750
Fragments arranged by size:
4750 548 346 119 28
BssSI C'ACGA_G
Cuts at: 3851 4863 3851
Size: 1012 4779
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5791
BstBI TT'CG_AA
Cuts at: 4595 4595
Size: 5791
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5791
BstXI CCAn_nnnn'nTGG
Cuts at: 2494 2494
Size: 5791
BstYI r'GATC_y
Cuts at: 1972 2230 2764 3121 3488 3873 3890 5342 5428
Size: 258 534 357 367 385 17 1452 86
Cuts at: 5428 5440 5786 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 534 385 367 357 346 258
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2142 2552 2662 513
Size: 552 939 138 410 110 3642
Fragments arranged by size:
3642 939 552 410 138 110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2633 487
Size: 683 56 1407 3645
Fragments arranged by size:
3645 1407 683 56
BtsI GCAGTG_nn'
Cuts at: 3307 4101 4121 3307
Size: 794 20 4977
Fragments arranged by size:
4977 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5791
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5756
DraI TTT'AAA
Cuts at: 3095 3095
Size: 5791
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4798 809
Size: 507 3482 1802
Fragments arranged by size:
3482 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3238 4127
Size: 54 1425 50 275 18 177 1231 889
Cuts at: 4127 5756 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1231 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3238 1537
Size: 275 1426 4090
Fragments arranged by size:
4090 1426 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2100 2184 2663 3721 1353
Size: 747 84 479 1058 3423
Fragments arranged by size:
3423 1058 747 479 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2313 4378 4752 4898 387
Size: 1064 138 724 2065 374 146 1280
Fragments arranged by size:
2065 1280 1064 724 374 146 138
EcoICRI GAG'CTC
Cuts at: 2883 2883
Size: 5791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2451 2654 2912 2977 3854 4451 5238
Size: 393 819 203 258 65 877 597 787
Cuts at: 5238 5506 1239
Size: 268 1524
Fragments arranged by size:
1524 877 819 787 597 393 268 258
203 65
EcoO109I rG'GnC_Cy
Cuts at: 2057 2129 2224 2519 2675 2723 2057
Size: 72 95 295 156 48 5125
Fragments arranged by size:
5125 295 156 95 72 48
EcoRI G'AATT_C
Cuts at: 3100 3478 3100
Size: 378 5413
FspI TGC'GCA
Cuts at: 2906 4297 2906
Size: 1391 4400
HaeII r_GCGC'y
Cuts at: 1678 2101 2297 2495 2501 2529 2589 2633 3016
Size: 423 196 198 6 28 60 44 383
Cuts at: 3016 3085 4938 1678
Size: 69 1853 2531
Fragments arranged by size:
2531 1853 423 383 198 196 69 60
44 28 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2272 2304 4432 4464 4506
Size: 32 135 32 700 32 2128 32 42
Cuts at: 4506 4538 1373
Size: 32 2626
Fragments arranged by size:
2626 2128 700 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3135 3381 669
Size: 988 281 1197 246 3079
Fragments arranged by size:
3079 1197 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5791
HpaI GTT'AAC
Cuts at: 3381 3381
Size: 5791
KasI G'GCGC_C
Cuts at: 2293 2525 2585 2629 2293
Size: 232 60 44 5455
Fragments arranged by size:
5455 232 60 44
KpnI G_GTAC'C
Cuts at: 2614 3116 2614
Size: 502 5289
MfeI C'AATT_G
Cuts at: 3390 3390
Size: 5791
MluI A'CGCG_T
Cuts at: 5667 5667
Size: 5791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4905 5089 1305
Size: 3600 184 2007
Fragments arranged by size:
3600 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3792
Fragments arranged by size:
3792 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3749 4108 4267 518
Size: 648 2583 359 159 2042
Fragments arranged by size:
2583 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2554 2664 3024 3207 3884 5032 5277 1606
Size: 948 110 360 183 677 1148 245 2120
Fragments arranged by size:
2120 1148 948 677 360 245 183 110
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 5791
NarI GG'CG_CC
Cuts at: 2294 2526 2586 2630 2294
Size: 232 60 44 5455
Fragments arranged by size:
5455 232 60 44
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4300
Fragments arranged by size:
4300 939 552
NdeI CA'TA_TG
Cuts at: 387 2350 387
Size: 1963 3828
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 5791
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5791
NotI GC'GGCC_GC
Cuts at: 3238 3238
Size: 5791
NspI r_CATG'y
Cuts at: 1132 1229 3149 4694 1132
Size: 97 1920 1545 2229
Fragments arranged by size:
2229 1920 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2053 1348
Size: 705 5086
PciI A'CATG_T
Cuts at: 4690 4690
Size: 5791
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5791
PfoI T'CCnGG_A
Cuts at: 2064 2064
Size: 5791
PmeI GTTT'AAAC
Cuts at: 3095 3095
Size: 5791
PmlI CAC'GTG
Cuts at: 2161 2844 2940 3041 2161
Size: 683 96 101 4911
Fragments arranged by size:
4911 683 101 96
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 5373 5405 3860
Size: 32 1481 32 4246
Fragments arranged by size:
4246 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2129 2224 2675 2723 2129
Size: 95 451 48 5197
Fragments arranged by size:
5197 451 95 48
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5791
PsiI TTA'TAA
Cuts at: 3361 3361
Size: 5791
PspOMI G'GGCC_C
Cuts at: 2056 2122 2254 2452 2519 2056
Size: 66 132 198 67 5328
Fragments arranged by size:
5328 198 132 67 66
PstI C_TGCA'G
Cuts at: 830 2761 3143 830
Size: 1931 382 3478
Fragments arranged by size:
3478 1931 382
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5791
RsrII CG'GwC_CG
Cuts at: 2556 2556
Size: 5791
SacI G_AGCT'C
Cuts at: 2885 2885
Size: 5791
SacII CC_GC'GG
Cuts at: 2555 2665 2555
Size: 110 5681
SalI G'TCGA_C
Cuts at: 1936 3133 1936
Size: 1197 4594
SanDI GG'GwC_CC
Cuts at: 2675 2723 2675
Size: 48 5743
SapI GCTCTTCn'nnn_
Cuts at: 2663 2663
Size: 5791
SbfI CC_TGCA'GG
Cuts at: 3143 3143
Size: 5791
ScaI AGT'ACT
Cuts at: 4039 5751 4039
Size: 1712 4079
SfcI C'TryA_G
Cuts at: 826 1045 2757 3139 4274 4955 5146 826
Size: 219 1712 382 1135 681 191 1471
Fragments arranged by size:
1712 1471 1135 681 382 219 191
SfoI GGC'GCC
Cuts at: 2295 2527 2587 2631 2295
Size: 232 60 44 5455
Fragments arranged by size:
5455 232 60 44
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5791
SmaI CCC'GGG
Cuts at: 1872 2127 2182 2364 2432 2517 2728 2856 3118
Size: 255 55 182 68 85 211 128 262
Cuts at: 3118 1872
Size: 4545
Fragments arranged by size:
4545 262 255 211 182 128 85 68
55
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2087 3895 4796 5058 820
Size: 197 926 144 1808 901 262 1553
Fragments arranged by size:
1808 1553 926 901 262 197 144
SnaBI TAC'GTA
Cuts at: 493 3582 493
Size: 3089 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5791
SphI G_CATG'C
Cuts at: 1132 3149 1132
Size: 2017 3774
SrfI GCCC'GGGC
Cuts at: 2432 2432
Size: 5791
SspI AAT'ATT
Cuts at: 5 52 3715 5
Size: 47 3663 2081
Fragments arranged by size:
3663 2081 47
StuI AGG'CCT
Cuts at: 1517 2306 1517
Size: 789 5002
StyI C'CwwG_G
Cuts at: 513 1065 2004 2487 2641 3230 513
Size: 552 939 483 154 589 3074
Fragments arranged by size:
3074 939 589 552 483 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2398 3818 3835 3988 4173 4618 325
Size: 1255 818 1420 17 153 185 445 1498
Fragments arranged by size:
1498 1420 1255 818 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4037 5749 96
Size: 274 80 33 51 1443 2060 1712 138
Fragments arranged by size:
2060 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5751 5783 5751
Size: 32 5759
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5791
XbaI T'CTAG_A
Cuts at: 3127 3127
Size: 5791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2224 2224
Size: 5791
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5791
XmaI C'CCGG_G
Cuts at: 1870 2125 2180 2362 2430 2515 2726 2854 3116
Size: 255 55 182 68 85 211 128 262
Cuts at: 3116 1870
Size: 4545
Fragments arranged by size:
4545 262 255 211 182 128 85 68
55
XmnI GAAnn'nnTTC
Cuts at: 3920 3920
Size: 5791
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3599 276
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PspXI PsrI PvuII SexAI
SfiI SwaI