Restriction map for
pFN21AB7617
(Circular) (Six-base) MAPSORT of: pFN21AB7617.seq Check: 3085 from: 1 to: 5626
pFN21AB7617 5626 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3436 278
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2969 1937
Size: 1032 4594
Acc65I G'GTAC_C
Cuts at: 2947 2947
Size: 5626
AclI AA'CG_TT
Cuts at: 1563 3753 4126 1563
Size: 2190 373 3063
Fragments arranged by size:
3063 2190 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3689 5073 5341 1632
Size: 2057 1384 268 1917
Fragments arranged by size:
2057 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2039 820
Size: 197 1022 4407
Fragments arranged by size:
4407 1022 197
AflIII A'CryG_T
Cuts at: 1203 4525 5502 1203
Size: 3322 977 1327
Fragments arranged by size:
3322 1327 977
AgeI A'CCGG_T
Cuts at: 4457 4457
Size: 5626
AhdI GACnn_n'nnGTC
Cuts at: 2599 4355 2599
Size: 1756 3870
AleI CACnn'nnGTG
Cuts at: 1166 2624 1166
Size: 1458 4168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5626
AlwNI CAG_nnn'CTG
Cuts at: 3369 4941 3369
Size: 1572 4054
ApaLI G'TGCA_C
Cuts at: 3683 4839 3683
Size: 1156 4470
ApoI r'AATT_y
Cuts at: 1493 2034 2060 2174 2213 2431 2493 2935 3161
Size: 541 26 114 39 218 62 442 226
Cuts at: 3161 3313 5422 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 541 442 226 218 152 114
62 39 26
AseI AT'TA_AT
Cuts at: 160 1032 4180 160
Size: 872 3148 1606
Fragments arranged by size:
3148 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
AvaI C'yCGr_G
Cuts at: 1870 1943 2951 1870
Size: 73 1008 4545
Fragments arranged by size:
4545 1008 73
BamHI G'GATC_C
Cuts at: 2956 3323 2956
Size: 367 5259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2947 4402 618
Size: 325 217 627 1160 1455 1842
Fragments arranged by size:
1842 1455 1160 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2285 2945 1391
Size: 894 660 4072
Fragments arranged by size:
4072 894 660
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4655
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3817 3851 1903
Size: 34 1880 34 3678
Fragments arranged by size:
3678 1880 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3518 4728 3518
Size: 1210 4416
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5626
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4237 136
Size: 107 122 71 631 605 2565 1525
Fragments arranged by size:
2565 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5621 5621
Size: 5626
BlpI GC'TnA_GC
Cuts at: 2540 3043 2540
Size: 503 5123
Bme1580I G_kGCm'C
Cuts at: 1790 3687 4843 1790
Size: 1897 1156 2573
Fragments arranged by size:
2573 1897 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5626
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3357 4315 442
Size: 416 53 83 399 24 1940 958 1753
Fragments arranged by size:
1940 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2423 4286 1239
Size: 1184 1863 2579
Fragments arranged by size:
2579 1863 1184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3751 4616 4914 3751
Size: 865 298 4463
Fragments arranged by size:
4463 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4289 882
Size: 617 2790 2219
Fragments arranged by size:
2790 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3417 493
Size: 711 2213 2702
Fragments arranged by size:
2702 2213 711
BsaBI GATnn'nnATC
Cuts at: 2397 5620 2397
Size: 3223 2403
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3433 3815 4450 275
Size: 53 83 186 936 1900 382 635 1451
Fragments arranged by size:
1900 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4059 4457 4731 4878 5289 4059
Size: 398 274 147 411 4396
Fragments arranged by size:
4396 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2695 2725 1178
Size: 30 165 30 1292 30 4079
Fragments arranged by size:
4079 1292 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5626
BseYI C'CCAG_C
Cuts at: 4829 5325 4829
Size: 496 5130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2631 1163
Size: 573 895 4158
Fragments arranged by size:
4158 895 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3076 3837 4865 1540
Size: 275 186 1075 761 1028 2301
Fragments arranged by size:
2301 1075 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2285 3687 3772 4843 2285
Size: 1402 85 1071 3068
Fragments arranged by size:
3068 1402 1071 85
BsmI GAATG_Cn'
Cuts at: 2047 2796 3137 3230 2047
Size: 749 341 93 4443
Fragments arranged by size:
4443 749 341 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2285 2945 3687 3772 4843 1391
Size: 399 495 660 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 660 495 399 85
BspEI T'CCGG_A
Cuts at: 5289 5289
Size: 5626
BspHI T'CATG_A
Cuts at: 3515 5245 3515
Size: 1730 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2699 2981 844
Size: 1855 282 3489
Fragments arranged by size:
3489 1855 282
BsrBI CCG'CTC
Cuts at: 1394 1823 3513 1394
Size: 429 1690 3507
Fragments arranged by size:
3507 1690 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2504 4121 4295 65
Size: 1183 1256 1617 174 1396
Fragments arranged by size:
1617 1396 1256 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4270 4457 1700
Size: 110 2460 187 2869
Fragments arranged by size:
2869 2460 187 110
BsrGI T'GTAC_A
Cuts at: 96 2380 96
Size: 2284 3342
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5626
BssSI C'ACGA_G
Cuts at: 3686 4698 3686
Size: 1012 4614
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5626
BstBI TT'CG_AA
Cuts at: 4430 4430
Size: 5626
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5626
BstYI r'GATC_y
Cuts at: 1972 2133 2956 3323 3708 3725 5177 5263 5275
Size: 161 823 367 385 17 1452 86 12
Cuts at: 5275 5621 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 823 385 367 346 161 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4135
Fragments arranged by size:
4135 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4887
Fragments arranged by size:
4887 683 56
BtsI GCAGTG_nn'
Cuts at: 3142 3936 3956 3142
Size: 794 20 4812
Fragments arranged by size:
4812 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5626
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5591
DraI TTT'AAA
Cuts at: 2930 2930
Size: 5626
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4633 809
Size: 507 3317 1802
Fragments arranged by size:
3317 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2412 3073 3962
Size: 54 1425 50 275 18 582 661 889
Cuts at: 3962 5591 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 661 582 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3073 1537
Size: 275 1261 4090
Fragments arranged by size:
4090 1261 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3556 1353
Size: 2203 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2583 4213 4587 4733 387
Size: 1064 138 994 1630 374 146 1280
Fragments arranged by size:
1630 1280 1064 994 374 146 138
EcoICRI GAG'CTC
Cuts at: 2283 2283
Size: 5626
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 3689 4286 5073 5341 1239
Size: 393 791 1266 597 787 268 1524
Fragments arranged by size:
1524 1266 791 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2174 2935 3313 2174
Size: 761 378 4487
Fragments arranged by size:
4487 761 378
FspI TGC'GCA
Cuts at: 4132 4132
Size: 5626
HaeII r_GCGC'y
Cuts at: 1678 4773 1678
Size: 3095 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2359 2391 2585 2617 4267
Size: 32 135 32 787 32 194 32 1650
Cuts at: 4267 4299 4341 4373 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1650 787 194 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2900 2970 3216 669
Size: 988 281 962 70 246 3079
Fragments arranged by size:
3079 988 962 281 246 70
HindIII A'AGCT_T
Cuts at: 748 2141 748
Size: 1393 4233
HpaI GTT'AAC
Cuts at: 3216 3216
Size: 5626
KpnI G_GTAC'C
Cuts at: 2951 2951
Size: 5626
MfeI C'AATT_G
Cuts at: 3225 3225
Size: 5626
MluI A'CGCG_T
Cuts at: 5502 5502
Size: 5626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4740 4924 1305
Size: 3435 184 2007
Fragments arranged by size:
3435 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3804
Fragments arranged by size:
3804 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2624 2801 3584 3943 4102 518
Size: 648 1458 177 783 359 159 2042
Fragments arranged by size:
2042 1458 783 648 359 177 159
MspA1I CmG'CkG
Cuts at: 1606 3042 3719 4867 5112 1606
Size: 1436 677 1148 245 2120
Fragments arranged by size:
2120 1436 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4135
Fragments arranged by size:
4135 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5626
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5626
NotI GC'GGCC_GC
Cuts at: 3073 3073
Size: 5626
NspI r_CATG'y
Cuts at: 1132 1229 2984 4529 1132
Size: 97 1755 1545 2229
Fragments arranged by size:
2229 1755 1545 97
PacI TTA_AT'TAA
Cuts at: 2832 2832
Size: 5626
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5626
PciI A'CATG_T
Cuts at: 4525 4525
Size: 5626
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5626
PfoI T'CCnGG_A
Cuts at: 2603 2603
Size: 5626
PmeI GTTT'AAAC
Cuts at: 2930 2930
Size: 5626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 5208 5240 3695
Size: 32 1481 32 4081
Fragments arranged by size:
4081 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5626
PsiI TTA'TAA
Cuts at: 2066 3196 2066
Size: 1130 4496
PstI C_TGCA'G
Cuts at: 830 2978 830
Size: 2148 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5626
SacI G_AGCT'C
Cuts at: 2285 2285
Size: 5626
SalI G'TCGA_C
Cuts at: 1936 2968 1936
Size: 1032 4594
SbfI CC_TGCA'GG
Cuts at: 2978 2978
Size: 5626
ScaI AGT'ACT
Cuts at: 3874 5586 3874
Size: 1712 3914
SfcI C'TryA_G
Cuts at: 826 1045 2974 4109 4790 4981 826
Size: 219 1929 1135 681 191 1471
Fragments arranged by size:
1929 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5626
SmaI CCC'GGG
Cuts at: 1872 2953 1872
Size: 1081 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2039 3730 4631 4893 820
Size: 197 926 96 1691 901 262 1553
Fragments arranged by size:
1691 1553 926 901 262 197 96
SnaBI TAC'GTA
Cuts at: 493 3417 493
Size: 2924 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5626
SphI G_CATG'C
Cuts at: 1132 2984 1132
Size: 1852 3774
SspI AAT'ATT
Cuts at: 5 52 2116 3550 5
Size: 47 2064 1434 2081
Fragments arranged by size:
2081 2064 1434 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5626
StyI C'CwwG_G
Cuts at: 513 1065 2004 3065 513
Size: 552 939 1061 3074
Fragments arranged by size:
3074 1061 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3653 3670 3823 4008 4453 325
Size: 1255 2073 17 153 185 445 1498
Fragments arranged by size:
2073 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2380 3872 5584
Size: 274 80 33 51 1443 403 1492 1712
Cuts at: 5584 96
Size: 138
Fragments arranged by size:
1712 1492 1443 403 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5586 5618 5586
Size: 32 5594
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5626
XbaI T'CTAG_A
Cuts at: 2962 2962
Size: 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5626
XmaI C'CCGG_G
Cuts at: 1870 2951 1870
Size: 1081 4545
XmnI GAAnn'nnTTC
Cuts at: 2796 3755 2796
Size: 959 4667
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3434 276
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI