Restriction map for
pFN21AB7614
(Circular) (Six-base) MAPSORT of: pFN21AB7614.seq Check: 4602 from: 1 to: 5785
pFN21AB7614 5785 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3128 1937
Size: 1191 4594
Acc65I G'GTAC_C
Cuts at: 2966 3106 2966
Size: 140 5645
AclI AA'CG_TT
Cuts at: 1563 3084 3912 4285 1563
Size: 1521 828 373 3063
Fragments arranged by size:
3063 1521 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2483 2639 3848 5232 5500 1632
Size: 851 156 1209 1384 268 1917
Fragments arranged by size:
1917 1384 1209 851 268 156
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 2592 4684 5661 1203
Size: 1389 2092 977 1327
Fragments arranged by size:
2092 1389 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 4514 4514
Size: 5785
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2732 2764 2732
Size: 32 5753
AlwNI CAG_nnn'CTG
Cuts at: 2168 3528 5100 2168
Size: 1360 1572 2853
Fragments arranged by size:
2853 1572 1360
ApaI G_GGCC'C
Cuts at: 2238 2958 2238
Size: 720 5065
ApaLI G'TGCA_C
Cuts at: 3842 4998 3842
Size: 1156 4629
ApoI r'AATT_y
Cuts at: 1493 2743 3094 3320 3472 5581 1493
Size: 1250 351 226 152 2109 1697
Fragments arranged by size:
2109 1697 1250 351 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 2441 2810 3110 1870
Size: 73 498 369 300 4545
Fragments arranged by size:
4545 498 369 300 73
BamHI G'GATC_C
Cuts at: 3115 3482 3115
Size: 367 5418
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2363 2847 2966 3106 4561
Size: 325 217 627 576 484 119 140 1455
Cuts at: 4561 618
Size: 1842
Fragments arranged by size:
1842 1455 627 576 484 325 217 140
119
BanII G_rGCy'C
Cuts at: 1391 2238 2346 2511 2811 2958 2987 3068 3104
Size: 847 108 165 300 147 29 81 36
Cuts at: 3104 1391
Size: 4072
Fragments arranged by size:
4072 847 300 165 147 108 81 36
29
BbeI G_GCGC'C
Cuts at: 2851 2851
Size: 5785
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4814
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2432 2466 3976 4010 1903
Size: 34 495 34 1510 34 3678
Fragments arranged by size:
3678 1510 495 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3677 4887 3677
Size: 1210 4575
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5785
BfrBI ATG'CAT
Cuts at: 3076 3076
Size: 5785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2074 2856 4396
Size: 107 122 71 631 605 402 782 1540
Cuts at: 4396 136
Size: 1525
Fragments arranged by size:
1540 1525 782 631 605 402 122 107
71
BglII A'GATC_T
Cuts at: 5780 5780
Size: 5785
BlpI GC'TnA_GC
Cuts at: 2111 2579 3202 2111
Size: 468 623 4694
Fragments arranged by size:
4694 623 468
Bme1580I G_kGCm'C
Cuts at: 1790 2238 2262 2762 2958 3846 5002 1790
Size: 448 24 500 196 888 1156 2573
Fragments arranged by size:
2573 1156 888 500 448 196 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5785
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3516 4474 442
Size: 416 53 83 399 24 2099 958 1753
Fragments arranged by size:
2099 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2031 2151 2396 2411 2759 4445 1239
Size: 792 120 245 15 348 1686 2579
Fragments arranged by size:
2579 1686 792 348 245 120 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3910 4775 5073 3910
Size: 865 298 4622
Fragments arranged by size:
4622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2270 2916 4448 882
Size: 617 771 646 1532 2219
Fragments arranged by size:
2219 1532 771 646 617
BsaAI yAC'GTr
Cuts at: 493 1204 3576 493
Size: 711 2372 2702
Fragments arranged by size:
2702 2372 711
BsaBI GATnn'nnATC
Cuts at: 5779 5779
Size: 5785
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2848 3592 3974 4609
Size: 53 83 186 936 1315 744 382 635
Cuts at: 4609 275
Size: 1451
Fragments arranged by size:
1451 1315 936 744 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4218 4616 4890 5037 5448 4218
Size: 398 274 147 411 4555
Fragments arranged by size:
4555 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2295 2325 2732 2762 1178
Size: 30 165 30 892 30 407 30 4201
Fragments arranged by size:
4201 892 407 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 2998 1186
Size: 1234 578 3973
Fragments arranged by size:
3973 1234 578
BseYI C'CCAG_C
Cuts at: 2088 2796 2906 4988 5484 2088
Size: 708 110 2082 496 2389
Fragments arranged by size:
2389 2082 708 496 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2332 2705 2982 1163
Size: 573 596 373 277 3966
Fragments arranged by size:
3966 596 573 373 277
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3235 3996 5024 1540
Size: 275 186 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3846 3931 5002 3846
Size: 85 1071 4629
Fragments arranged by size:
4629 1071 85
BsmI GAATG_Cn'
Cuts at: 3076 3296 3389 3076
Size: 220 93 5472
Fragments arranged by size:
5472 220 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2238 2262 2346 2511 2762 2811 2958
Size: 399 448 24 84 165 251 49 147
Cuts at: 2958 2987 3068 3104 3846 3931 5002 1391
Size: 29 81 36 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 448 399 251 165 147
85 84 81 49 36 29 24
BspEI T'CCGG_A
Cuts at: 5448 5448
Size: 5785
BspHI T'CATG_A
Cuts at: 3674 5404 3674
Size: 1730 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3140 844
Size: 2296 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3672 1394
Size: 429 1849 3507
Fragments arranged by size:
3507 1849 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4280 4454 65
Size: 1183 3032 174 1396
Fragments arranged by size:
3032 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2844 4429 4616 1700
Size: 110 1034 1585 187 2869
Fragments arranged by size:
2869 1585 1034 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 2681 3845 4857 2681
Size: 1164 1012 3609
Fragments arranged by size:
3609 1164 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5785
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5785
BstXI CCAn_nnnn'nTGG
Cuts at: 2804 2804
Size: 5785
BstYI r'GATC_y
Cuts at: 1972 3115 3482 3867 3884 5336 5422 5434 5780
Size: 1143 367 385 17 1452 86 12 346
Cuts at: 5780 1972
Size: 1977
Fragments arranged by size:
1977 1452 1143 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2289 3024 2289
Size: 735 5050
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4294
Fragments arranged by size:
4294 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2776 487
Size: 683 56 1550 3496
Fragments arranged by size:
3496 1550 683 56
BtsI GCAGTG_nn'
Cuts at: 2762 2771 2994 3301 4095 4115 2762
Size: 9 223 307 794 20 4432
Fragments arranged by size:
4432 794 307 223 20 9
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 3089 3089
Size: 5785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3232 4121 5750
Size: 54 1425 50 275 18 1402 889 1629
Cuts at: 5750 8
Size: 43
Fragments arranged by size:
1629 1425 1402 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2465 3715 1353
Size: 1112 1250 3423
Fragments arranged by size:
3423 1250 1112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4372 4746 4892 387
Size: 1064 138 2783 374 146 1280
Fragments arranged by size:
2783 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2151 2396 2411 2483 2639 2759
Size: 393 399 120 245 15 72 156 120
Cuts at: 2759 3848 4445 5232 5500 1239
Size: 1089 597 787 268 1524
Fragments arranged by size:
1524 1089 787 597 399 393 268 245
156 120 120 72 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2130 2684 2919 2130
Size: 554 235 4996
Fragments arranged by size:
4996 554 235
EcoO109I rG'GnC_Cy
Cuts at: 2123 2235 2331 2954 2955 2123
Size: 112 96 623 1 4953
Fragments arranged by size:
4953 623 112 96 1
EcoRI G'AATT_C
Cuts at: 3094 3472 3094
Size: 378 5407
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 2851 4932 1678
Size: 1173 2081 2531
Fragments arranged by size:
2531 2081 1173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2318 2350 4426 4458 4500
Size: 32 135 32 746 32 2076 32 42
Cuts at: 4500 4532 1373
Size: 32 2626
Fragments arranged by size:
2626 2076 746 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3129 3375 669
Size: 988 281 1191 246 3079
Fragments arranged by size:
3079 1191 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2113 748
Size: 1365 4420
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KasI G'GCGC_C
Cuts at: 2847 2847
Size: 5785
KpnI G_GTAC'C
Cuts at: 2970 3110 2970
Size: 140 5645
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2224 4899 5083 1305
Size: 919 2675 184 2007
Fragments arranged by size:
2675 2007 919 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3963
Fragments arranged by size:
3963 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3743 4102 4261 518
Size: 648 2577 359 159 2042
Fragments arranged by size:
2577 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3201 3878 5026 5271 1606
Size: 1595 677 1148 245 2120
Fragments arranged by size:
2120 1595 1148 677 245
NarI GG'CG_CC
Cuts at: 2848 2848
Size: 5785
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4294
Fragments arranged by size:
4294 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NsiI A_TGCA'T
Cuts at: 3078 3078
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 2596 3143 4688 1132
Size: 97 1367 547 1545 2229
Fragments arranged by size:
2229 1545 1367 547 97
PasI CC'CwG_GG
Cuts at: 1348 2482 2515 2951 1348
Size: 1134 33 436 4182
Fragments arranged by size:
4182 1134 436 33
PciI A'CATG_T
Cuts at: 2592 4684 2592
Size: 2092 3693
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2054 2978 1108
Size: 946 924 3915
Fragments arranged by size:
3915 946 924
PfoI T'CCnGG_A
Cuts at: 2128 2128
Size: 5785
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2732 2764 3854 3886 5367 5399 2732
Size: 32 1090 32 1481 32 3118
Fragments arranged by size:
3118 1481 1090 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2932 1613
Size: 1319 4466
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PspOMI G'GGCC_C
Cuts at: 2234 2954 2234
Size: 720 5065
PspXI vC'TCGA_Gb
Cuts at: 2441 2441
Size: 5785
PstI C_TGCA'G
Cuts at: 830 2433 2577 2619 2757 2778 3137 830
Size: 1603 144 42 138 21 359 3478
Fragments arranged by size:
3478 1603 359 144 138 42 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SfcI C'TryA_G
Cuts at: 826 1045 2429 2573 2615 2753 2774 3133 4268
Size: 219 1384 144 42 138 21 359 1135
Cuts at: 4268 4949 5140 826
Size: 681 191 1471
Fragments arranged by size:
1471 1384 1135 681 359 219 191 144
138 42 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 2074 2074
Size: 5785
SfoI GGC'GCC
Cuts at: 2849 2849
Size: 5785
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2441 2810 3889 4790 5052 820
Size: 197 926 498 369 1079 901 262 1553
Fragments arranged by size:
1553 1079 926 901 498 369 262 197
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5785
StyI C'CwwG_G
Cuts at: 513 1065 2004 2119 2263 3224 513
Size: 552 939 115 144 961 3074
Fragments arranged by size:
3074 961 939 552 144 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2614 3001 3812 3829 3982 4167 4612
Size: 1255 1034 387 811 17 153 185 445
Cuts at: 4612 325
Size: 1498
Fragments arranged by size:
1498 1255 1034 811 445 387 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3003 4031 5743
Size: 274 80 33 51 1443 1026 1028 1712
Cuts at: 5743 96
Size: 138
Fragments arranged by size:
1712 1443 1028 1026 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2295 2327 5745 5777 2295
Size: 32 3418 32 2303
Fragments arranged by size:
3418 2303 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5785
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2126 2126
Size: 5785
XhoI C'TCGA_G
Cuts at: 1943 2441 2810 1943
Size: 498 369 4918
Fragments arranged by size:
4918 498 369
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 2537 2652 2747 3036 3914 2537
Size: 115 95 289 878 4408
Fragments arranged by size:
4408 878 289 115 95
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI SalI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI Bpu10I BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI PacI PmlI PpuMI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SrfI SwaI