Restriction map for
pFN21AB7609
(Circular) (Six-base) MAPSORT of: pFN21AB7609.seq Check: 6571 from: 1 to: 5605
pFN21AB7609 5605 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3415 278
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2948 1937
Size: 1011 4594
Acc65I G'GTAC_C
Cuts at: 2926 2926
Size: 5605
AclI AA'CG_TT
Cuts at: 1563 2904 3732 4105 1563
Size: 1341 828 373 3063
Fragments arranged by size:
3063 1341 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 2698 3668 5052 5320 1632
Size: 725 341 970 1384 268 1917
Fragments arranged by size:
1917 1384 970 725 341 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 4504 5481 1203
Size: 3301 977 1327
Fragments arranged by size:
3301 1327 977
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2288 1184
Size: 1104 4501
AlwNI CAG_nnn'CTG
Cuts at: 2335 3348 4920 2335
Size: 1013 1572 3020
Fragments arranged by size:
3020 1572 1013
ApaLI G'TGCA_C
Cuts at: 3662 4818 3662
Size: 1156 4449
ApoI r'AATT_y
Cuts at: 1493 2136 2636 2826 2864 2914 3140 3292 5401
Size: 643 500 190 38 50 226 152 2109
Cuts at: 5401 1493
Size: 1697
Fragments arranged by size:
2109 1697 643 500 226 190 152 50
38
AseI AT'TA_AT
Cuts at: 160 1032 2681 4159 160
Size: 872 1649 1478 1606
Fragments arranged by size:
1649 1606 1478 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2286 2614 2880 2930 1870
Size: 73 343 328 266 50 4545
Fragments arranged by size:
4545 343 328 266 73 50
BamHI G'GATC_C
Cuts at: 2935 3302 2935
Size: 367 5238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2926 4381 618
Size: 325 217 627 1139 1455 1842
Fragments arranged by size:
1842 1455 1139 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2512 2924 1391
Size: 1121 412 4072
Fragments arranged by size:
4072 1121 412
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2700 2704 2857 928
Size: 971 801 4 153 3676
Fragments arranged by size:
3676 971 801 153 4
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3796 3830 1903
Size: 34 1859 34 3678
Fragments arranged by size:
3678 1859 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2231 3497 4707 2231
Size: 1266 1210 3129
Fragments arranged by size:
3129 1266 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4216 136
Size: 107 122 71 631 605 2544 1525
Fragments arranged by size:
2544 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2514 5600 2514
Size: 3086 2519
BlpI GC'TnA_GC
Cuts at: 2742 2792 3022 2742
Size: 50 230 5325
Fragments arranged by size:
5325 230 50
Bme1580I G_kGCm'C
Cuts at: 1790 3666 4822 1790
Size: 1876 1156 2573
Fragments arranged by size:
2573 1876 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3336 4294 442
Size: 416 53 83 399 24 1919 958 1753
Fragments arranged by size:
1919 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2640 2640
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 2852 4265 1239
Size: 926 687 1413 2579
Fragments arranged by size:
2579 1413 926 687
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3730 4595 4893 3730
Size: 865 298 4442
Fragments arranged by size:
4442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4268 882
Size: 617 2769 2219
Fragments arranged by size:
2769 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2094 3396 493
Size: 711 890 1302 2702
Fragments arranged by size:
2702 1302 890 711
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2709 3412 3794 4429
Size: 53 83 186 936 1176 703 382 635
Cuts at: 4429 275
Size: 1451
Fragments arranged by size:
1451 1176 936 703 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4038 4436 4710 4857 5268 4038
Size: 398 274 147 411 4375
Fragments arranged by size:
4375 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2010 2040 2072 2102 1178
Size: 30 165 30 607 30 32 30 4681
Fragments arranged by size:
4681 607 165 32 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2303 1186
Size: 1117 4488
BseYI C'CCAG_C
Cuts at: 4808 5304 4808
Size: 496 5109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2374 1163
Size: 573 638 4394
Fragments arranged by size:
4394 638 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3055 3816 4844 1540
Size: 275 186 1054 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2256 3666 3751 4822 2256
Size: 1410 85 1071 3039
Fragments arranged by size:
3039 1410 1071 85
BsmI GAATG_Cn'
Cuts at: 2881 3116 3209 2881
Size: 235 93 5277
Fragments arranged by size:
5277 235 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2256 2512 2924 3666 3751 4822 1391
Size: 399 466 256 412 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 466 412 399 256 85
BspEI T'CCGG_A
Cuts at: 5268 5268
Size: 5605
BspHI T'CATG_A
Cuts at: 2421 3494 5224 2421
Size: 1073 1730 2802
Fragments arranged by size:
2802 1730 1073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2960 844
Size: 2116 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3492 1394
Size: 429 1669 3507
Fragments arranged by size:
3507 1669 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2104 4100 4274 65
Size: 1183 856 1996 174 1396
Fragments arranged by size:
1996 1396 1183 856 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4249 4436 1700
Size: 110 2439 187 2869
Fragments arranged by size:
2869 2439 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5605
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2206 2011
Size: 195 5410
BstYI r'GATC_y
Cuts at: 1972 2514 2935 3302 3687 3704 5156 5242 5254
Size: 542 421 367 385 17 1452 86 12
Cuts at: 5254 5600 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 542 421 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5053
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4866
Fragments arranged by size:
4866 683 56
BtsI GCAGTG_nn'
Cuts at: 2742 3121 3915 3935 2742
Size: 379 794 20 4412
Fragments arranged by size:
4412 794 379 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5570
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4612 809
Size: 507 3296 1802
Fragments arranged by size:
3296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2347 3052 3941
Size: 54 1425 50 275 18 517 705 889
Cuts at: 3941 5570 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 705 517 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 1537
Size: 275 1240 4090
Fragments arranged by size:
4090 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3535 1353
Size: 2182 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4192 4566 4712 387
Size: 1064 138 2603 374 146 1280
Fragments arranged by size:
2603 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2357 2698 2852 3668 4265 5052
Size: 393 533 192 341 154 816 597 787
Cuts at: 5052 5320 1239
Size: 268 1524
Fragments arranged by size:
1524 816 787 597 533 393 341 268
192 154
EcoRI G'AATT_C
Cuts at: 2636 2914 3292 2636
Size: 278 378 4949
Fragments arranged by size:
4949 378 278
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 4752 1678
Size: 3074 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2072 2104 4246 4278 4320
Size: 32 135 32 500 32 2142 32 42
Cuts at: 4320 4352 1373
Size: 32 2626
Fragments arranged by size:
2626 2142 500 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2949 3195 669
Size: 988 281 1011 246 3079
Fragments arranged by size:
3079 1011 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5605
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KpnI G_GTAC'C
Cuts at: 2930 2930
Size: 5605
MfeI C'AATT_G
Cuts at: 2215 3204 2215
Size: 989 4616
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2466 4719 4903 1305
Size: 1161 2253 184 2007
Fragments arranged by size:
2253 2007 1161 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3783
Fragments arranged by size:
3783 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3563 3922 4081 518
Size: 648 2397 359 159 2042
Fragments arranged by size:
2397 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2206 3021 3698 4846 5091 1606
Size: 600 815 677 1148 245 2120
Fragments arranged by size:
2120 1148 815 677 600 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5053
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2963 4508 1132
Size: 97 1734 1545 2229
Fragments arranged by size:
2229 1734 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5605
PciI A'CATG_T
Cuts at: 4504 4504
Size: 5605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5605
PfoI T'CCnGG_A
Cuts at: 2190 2190
Size: 5605
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PmlI CAC'GTG
Cuts at: 2094 2094
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 5187 5219 3674
Size: 32 1481 32 4060
Fragments arranged by size:
4060 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2264 2957 830
Size: 1434 693 3478
Fragments arranged by size:
3478 1434 693
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
PvuII CAG'CTG
Cuts at: 2206 2206
Size: 5605
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SbfI CC_TGCA'GG
Cuts at: 2957 2957
Size: 5605
ScaI AGT'ACT
Cuts at: 2150 3853 5565 2150
Size: 1703 1712 2190
Fragments arranged by size:
2190 1712 1703
SexAI A'CCwGG_T
Cuts at: 2163 2163
Size: 5605
SfcI C'TryA_G
Cuts at: 826 1045 2260 2317 2953 4088 4769 4960 826
Size: 219 1215 57 636 1135 681 191 1471
Fragments arranged by size:
1471 1215 1135 681 636 219 191 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5605
SmaI CCC'GGG
Cuts at: 1872 2288 2932 1872
Size: 416 644 4545
Fragments arranged by size:
4545 644 416
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3709 4610 4872 820
Size: 197 926 1766 901 262 1553
Fragments arranged by size:
1766 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5605
StyI C'CwwG_G
Cuts at: 513 1065 3044 513
Size: 552 1979 3074
Fragments arranged by size:
3074 1979 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2712 3632 3649 3802 3987 4432 325
Size: 1255 1132 920 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1132 920 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2148 3851 5563
Size: 274 80 33 51 1443 171 1703 1712
Cuts at: 5563 96
Size: 138
Fragments arranged by size:
1712 1703 1443 274 171 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5565 5597 5565
Size: 32 5573
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5605
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5605
XmaI C'CCGG_G
Cuts at: 1870 2286 2930 1870
Size: 416 644 4545
Fragments arranged by size:
4545 644 416
XmnI GAAnn'nnTTC
Cuts at: 3734 3734
Size: 5605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3413 276
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI XcmI