Restriction map for
pFN21AB7607
(Circular) (Six-base) MAPSORT of: pFN21AB7607.seq Check: 8608 from: 1 to: 5602
pFN21AB7607 5602 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2202 3412 278
Size: 53 83 186 936 666 1210 2468
Fragments arranged by size:
2468 1210 936 666 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2945 1937
Size: 1008 4594
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 3729 4102 1563
Size: 2166 373 3063
Fragments arranged by size:
3063 2166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2246 3665 5049 5317 1632
Size: 614 1419 1384 268 1917
Fragments arranged by size:
1917 1419 1384 614 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5405
AflIII A'CryG_T
Cuts at: 1203 4501 5478 1203
Size: 3298 977 1327
Fragments arranged by size:
3298 1327 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 4331 4331
Size: 5602
AleI CACnn'nnGTG
Cuts at: 1166 2393 2471 1166
Size: 1227 78 4297
Fragments arranged by size:
4297 1227 78
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5602
AlwNI CAG_nnn'CTG
Cuts at: 3345 4917 3345
Size: 1572 4030
ApaI G_GGCC'C
Cuts at: 2031 2699 2031
Size: 668 4934
ApaLI G'TGCA_C
Cuts at: 3659 4815 3659
Size: 1156 4446
ApoI r'AATT_y
Cuts at: 1493 2807 2834 2911 3137 3289 5398 1493
Size: 1314 27 77 226 152 2109 1697
Fragments arranged by size:
2109 1697 1314 226 152 77 27
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2927 1870
Size: 73 80 904 4545
Fragments arranged by size:
4545 904 80 73
BamHI G'GATC_C
Cuts at: 2932 3299 2932
Size: 367 5235
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2680 2780 2825 2923 4378
Size: 325 217 627 893 100 45 98 1455
Cuts at: 4378 618
Size: 1842
Fragments arranged by size:
1842 1455 893 627 325 217 100 98
45
BanII G_rGCy'C
Cuts at: 1391 2031 2498 2642 2699 2921 1391
Size: 640 467 144 57 222 4072
Fragments arranged by size:
4072 640 467 222 144 57
BbeI G_GCGC'C
Cuts at: 2784 2784
Size: 5602
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4631
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3793 3827 1903
Size: 34 1856 34 3678
Fragments arranged by size:
3678 1856 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3494 4704 3494
Size: 1210 4392
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2454 4213
Size: 107 122 71 631 605 337 445 1759
Cuts at: 4213 136
Size: 1525
Fragments arranged by size:
1759 1525 631 605 445 337 122 107
71
BglII A'GATC_T
Cuts at: 2895 5597 2895
Size: 2702 2900
BlpI GC'TnA_GC
Cuts at: 3019 3019
Size: 5602
Bme1580I G_kGCm'C
Cuts at: 1790 2031 2097 2372 2685 2699 2828 3663 4819
Size: 241 66 275 313 14 129 835 1156
Cuts at: 4819 1790
Size: 2573
Fragments arranged by size:
2573 1156 835 313 275 241 129 66
14
BmgBI CAC'GTC
Cuts at: 1318 2373 1318
Size: 1055 4547
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2061 2182 2740
Size: 416 53 83 399 24 644 121 558
Cuts at: 2740 3333 4291 442
Size: 593 958 1753
Fragments arranged by size:
1753 958 644 593 558 416 399 121
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2177 2186 2512 4262 1239
Size: 938 9 326 1750 2579
Fragments arranged by size:
2579 1750 938 326 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3727 4592 4890 3727
Size: 865 298 4439
Fragments arranged by size:
4439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2637 4265 882
Size: 617 1138 1628 2219
Fragments arranged by size:
2219 1628 1138 617
BsaAI yAC'GTr
Cuts at: 493 1204 3393 493
Size: 711 2189 2702
Fragments arranged by size:
2702 2189 711
BsaBI GATnn'nnATC
Cuts at: 5596 5596
Size: 5602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2199 2781 3409 3791
Size: 53 83 186 936 666 582 628 382
Cuts at: 3791 4426 275
Size: 635 1451
Fragments arranged by size:
1451 936 666 635 628 582 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2271 4035 4433 4707 4854 5265 2271
Size: 1764 398 274 147 411 2608
Fragments arranged by size:
2608 1764 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2159 2189 1178
Size: 30 165 30 756 30 4591
Fragments arranged by size:
4591 756 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 2228 1186
Size: 988 54 4560
Fragments arranged by size:
4560 988 54
BseYI C'CCAG_C
Cuts at: 2497 2534 2685 4805 5301 2497
Size: 37 151 2120 496 2798
Fragments arranged by size:
2798 2120 496 151 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5029
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3052 3813 4841 1540
Size: 275 186 1051 761 1028 2301
Fragments arranged by size:
2301 1051 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2642 3663 3748 4819 2642
Size: 1021 85 1071 3425
Fragments arranged by size:
3425 1071 1021 85
BsmI GAATG_Cn'
Cuts at: 3113 3206 3113
Size: 93 5509
BsmBI CGTCTCn'nnnn_
Cuts at: 2562 2562
Size: 5602
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2097 2372 2498 2642 2685 2699
Size: 399 241 66 275 126 144 43 14
Cuts at: 2699 2828 2921 3663 3748 4819 1391
Size: 129 93 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 275 241 144 129
126 93 85 66 43 14
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 3491 5221 3491
Size: 1730 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2957 844
Size: 2113 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3489 1394
Size: 429 1666 3507
Fragments arranged by size:
3507 1666 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4097 4271 65
Size: 1183 2849 174 1396
Fragments arranged by size:
2849 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4246 4433 1700
Size: 110 2436 187 2869
Fragments arranged by size:
2869 2436 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5602
BssSI C'ACGA_G
Cuts at: 3662 4674 3662
Size: 1012 4590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2177 794
Size: 1383 4219
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5602
BstXI CCAn_nnnn'nTGG
Cuts at: 2473 2777 2473
Size: 304 5298
BstYI r'GATC_y
Cuts at: 1972 2074 2895 2932 3299 3684 3701 5153 5239
Size: 102 821 37 367 385 17 1452 86
Cuts at: 5239 5251 5597 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 821 385 367 346 102 86
37 17 12
Bsu36I CC'TnA_GG
Cuts at: 2452 2520 2548 2452
Size: 68 28 5506
Fragments arranged by size:
5506 68 28
BtgI C'CryG_G
Cuts at: 513 1065 2466 2676 2727 2793 2820 2850 513
Size: 552 1401 210 51 66 27 30 3265
Fragments arranged by size:
3265 1401 552 210 66 51 30 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4863
Fragments arranged by size:
4863 683 56
BtsI GCAGTG_nn'
Cuts at: 2478 3118 3912 3932 2478
Size: 640 794 20 4148
Fragments arranged by size:
4148 794 640 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5567
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4609 809
Size: 507 3293 1802
Fragments arranged by size:
3293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2475 3049 3938
Size: 54 1425 50 275 18 645 574 889
Cuts at: 3938 5567 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 645 574 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 1537
Size: 275 1237 4090
Fragments arranged by size:
4090 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2617 3532 1353
Size: 1264 915 3423
Fragments arranged by size:
3423 1264 915
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2557 2611 4189 4563 4709 387
Size: 1064 138 968 54 1578 374 146 1280
Fragments arranged by size:
1578 1280 1064 968 374 146 138 54
EcoICRI GAG'CTC
Cuts at: 2640 2640
Size: 5602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2177 2186 2246 2512 3665 4262 5049
Size: 393 545 9 60 266 1153 597 787
Cuts at: 5049 5317 1239
Size: 268 1524
Fragments arranged by size:
1524 1153 787 597 545 393 268 266
60 9
EcoO109I rG'GnC_Cy
Cuts at: 2027 2028 2695 2732 2815 2027
Size: 1 667 37 83 4814
Fragments arranged by size:
4814 667 83 37 1
EcoRI G'AATT_C
Cuts at: 2807 2911 3289 2807
Size: 104 378 5120
Fragments arranged by size:
5120 378 104
FspI TGC'GCA
Cuts at: 4108 4108
Size: 5602
HaeII r_GCGC'y
Cuts at: 1678 2109 2784 4749 1678
Size: 431 675 1965 2531
Fragments arranged by size:
2531 1965 675 431
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4243 4275 4317 4349 1373
Size: 32 135 32 2671 32 42 32 2626
Fragments arranged by size:
2671 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2044 2946 3192 669
Size: 988 281 106 902 246 3079
Fragments arranged by size:
3079 988 902 281 246 106
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5602
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KasI G'GCGC_C
Cuts at: 2780 2780
Size: 5602
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4716 4900 1305
Size: 3411 184 2007
Fragments arranged by size:
3411 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2477 10
Size: 54 1425 343 645 3135
Fragments arranged by size:
3135 1425 645 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2393 2471 3560 3919 4078 518
Size: 648 1227 78 1089 359 159 2042
Fragments arranged by size:
2042 1227 1089 648 359 159 78
MspA1I CmG'CkG
Cuts at: 1606 2122 2530 2652 3018 3695 4843 5088 1606
Size: 516 408 122 366 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 516 408 366 245 122
NarI GG'CG_CC
Cuts at: 2781 2781
Size: 5602
NcoI C'CATG_G
Cuts at: 513 1065 2676 2850 513
Size: 552 1611 174 3265
Fragments arranged by size:
3265 1611 552 174
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5602
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2960 4505 1132
Size: 97 1731 1545 2229
Fragments arranged by size:
2229 1731 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2327 1348
Size: 979 4623
PciI A'CATG_T
Cuts at: 4501 4501
Size: 5602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5602
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 5184 5216 3671
Size: 32 1481 32 4057
Fragments arranged by size:
4057 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2732 2815 2732
Size: 83 5519
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 3172 3172
Size: 5602
PspOMI G'GGCC_C
Cuts at: 2027 2695 2027
Size: 668 4934
PstI C_TGCA'G
Cuts at: 830 2954 830
Size: 2124 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5602
SacI G_AGCT'C
Cuts at: 2642 2642
Size: 5602
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SapI GCTCTTCn'nnn_
Cuts at: 2617 2617
Size: 5602
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 3850 5562 3850
Size: 1712 3890
SfcI C'TryA_G
Cuts at: 826 1045 2428 2785 2950 4085 4766 4957 826
Size: 219 1383 357 165 1135 681 191 1471
Fragments arranged by size:
1471 1383 1135 681 357 219 191 165
SfoI GGC'GCC
Cuts at: 2782 2782
Size: 5602
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2025 2929 1872
Size: 153 904 4545
Fragments arranged by size:
4545 904 153
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3706 4607 4869 820
Size: 197 926 1763 901 262 1553
Fragments arranged by size:
1763 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2960 1132
Size: 1828 3774
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5602
StyI C'CwwG_G
Cuts at: 513 1065 2049 2340 2676 2850 3041 513
Size: 552 984 291 336 174 191 3074
Fragments arranged by size:
3074 984 552 336 291 191 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3629 3646 3799 3984 4429 325
Size: 1255 2049 17 153 185 445 1498
Fragments arranged by size:
2049 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3848 5560 96
Size: 274 80 33 51 1443 1871 1712 138
Fragments arranged by size:
1871 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2830 2862 5562 5594 2830
Size: 32 2700 32 2838
Fragments arranged by size:
2838 2700 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5602
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2857 2857
Size: 5602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5602
XmaI C'CCGG_G
Cuts at: 1870 2023 2927 1870
Size: 153 904 4545
Fragments arranged by size:
4545 904 153
XmnI GAAnn'nnTTC
Cuts at: 3731 3731
Size: 5602
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2200 3410 276
Size: 53 83 186 936 666 1210 2468
Fragments arranged by size:
2468 1210 936 666 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfiI SrfI SwaI