Restriction map for
pFN21AB7603
(Circular) (Six-base) MAPSORT of: pFN21AB7603.seq Check: 1685 from: 1 to: 5596
pFN21AB7603 5596 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 1937
Size: 1002 4594
Acc65I G'GTAC_C
Cuts at: 2211 2696 2917 2211
Size: 485 221 4890
Fragments arranged by size:
4890 485 221
AclI AA'CG_TT
Cuts at: 1563 3723 4096 1563
Size: 2160 373 3063
Fragments arranged by size:
3063 2160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2559 3659 5043 5311 1632
Size: 927 1100 1384 268 1917
Fragments arranged by size:
1917 1384 1100 927 268
AflII C'TTAA_G
Cuts at: 820 1017 2018 820
Size: 197 1001 4398
Fragments arranged by size:
4398 1001 197
AflIII A'CryG_T
Cuts at: 1203 4495 5472 1203
Size: 3292 977 1327
Fragments arranged by size:
3292 1327 977
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 4325 4325
Size: 5596
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5596
AlwNI CAG_nnn'CTG
Cuts at: 3339 4911 3339
Size: 1572 4024
ApaI G_GGCC'C
Cuts at: 2106 2106
Size: 5596
ApaLI G'TGCA_C
Cuts at: 3653 4809 3653
Size: 1156 4440
ApoI r'AATT_y
Cuts at: 1493 2882 2905 3131 3283 5392 1493
Size: 1389 23 226 152 2109 1697
Fragments arranged by size:
2109 1697 1389 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2098 2166 2261 2382 2921 1870
Size: 73 155 68 95 121 539 4545
Fragments arranged by size:
4545 539 155 121 95 73 68
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2211 2696 2917 4372 618
Size: 325 217 627 424 485 221 1455 1842
Fragments arranged by size:
1842 1455 627 485 424 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2106 2489 2915 1391
Size: 715 383 426 4072
Fragments arranged by size:
4072 715 426 383
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2375 928
Size: 971 476 4149
Fragments arranged by size:
4149 971 476
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 1903
Size: 34 1850 34 3678
Fragments arranged by size:
3678 1850 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5596
BfrBI ATG'CAT
Cuts at: 2653 2653
Size: 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4207 136
Size: 107 122 71 631 605 2535 1525
Fragments arranged by size:
2535 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5591 5591
Size: 5596
BlpI GC'TnA_GC
Cuts at: 3013 3013
Size: 5596
Bme1580I G_kGCm'C
Cuts at: 1790 2106 3657 4813 1790
Size: 316 1551 1156 2573
Fragments arranged by size:
2573 1551 1156 316
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5596
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3327 4285 442
Size: 416 53 83 399 24 1910 958 1753
Fragments arranged by size:
1910 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2361 2503 4256 1239
Size: 1122 142 1753 2579
Fragments arranged by size:
2579 1753 1122 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3721 4586 4884 3721
Size: 865 298 4433
Fragments arranged by size:
4433 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2347 4259 882
Size: 617 848 1912 2219
Fragments arranged by size:
2219 1912 848 617
BsaAI yAC'GTr
Cuts at: 493 1204 3387 493
Size: 711 2183 2702
Fragments arranged by size:
2702 2183 711
BsaBI GATnn'nnATC
Cuts at: 5590 5590
Size: 5596
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3403 3785 4420 275
Size: 53 83 186 936 1870 382 635 1451
Fragments arranged by size:
1870 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4029 4427 4701 4848 5259 4029
Size: 398 274 147 411 4366
Fragments arranged by size:
4366 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5371
Fragments arranged by size:
5371 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 2518 1186
Size: 1063 269 4264
Fragments arranged by size:
4264 1063 269
BseYI C'CCAG_C
Cuts at: 2488 2731 4799 5295 2488
Size: 243 2068 496 2789
Fragments arranged by size:
2789 2068 496 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2063 2294 1163
Size: 573 327 231 4465
Fragments arranged by size:
4465 573 327 231
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3046 3807 4835 1540
Size: 275 186 1045 761 1028 2301
Fragments arranged by size:
2301 1045 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2470 3657 3742 4813 2470
Size: 1187 85 1071 3253
Fragments arranged by size:
3253 1187 1071 85
BsmI GAATG_Cn'
Cuts at: 3107 3200 3107
Size: 93 5503
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2470 2489 2915 3657 3742 4813
Size: 399 316 364 19 426 742 85 1071
Cuts at: 4813 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 426 399 364 316 85
19
BspEI T'CCGG_A
Cuts at: 5259 5259
Size: 5596
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2266 2365 2951 844
Size: 1422 99 586 3489
Fragments arranged by size:
3489 1422 586 99
BsrBI CCG'CTC
Cuts at: 1394 1823 3483 1394
Size: 429 1660 3507
Fragments arranged by size:
3507 1660 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2451 4091 4265 65
Size: 1183 1203 1640 174 1396
Fragments arranged by size:
1640 1396 1203 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4240 4427 1700
Size: 110 2430 187 2869
Fragments arranged by size:
2869 2430 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5596
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5596
BssSI C'ACGA_G
Cuts at: 2875 3656 4668 2875
Size: 781 1012 3803
Fragments arranged by size:
3803 1012 781
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2198 794
Size: 1404 4192
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5596
BstXI CCAn_nnnn'nTGG
Cuts at: 2756 2756
Size: 5596
BstYI r'GATC_y
Cuts at: 1972 2512 2926 3293 3678 3695 5147 5233 5245
Size: 540 414 367 385 17 1452 86 12
Cuts at: 5245 5591 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 540 414 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2088 2691 513
Size: 552 1023 603 3418
Fragments arranged by size:
3418 1023 603 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4857
Fragments arranged by size:
4857 683 56
BtsI GCAGTG_nn'
Cuts at: 2470 3112 3906 3926 2470
Size: 642 794 20 4140
Fragments arranged by size:
4140 794 642 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5561
DraI TTT'AAA
Cuts at: 2900 2900
Size: 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2413 3043 3932
Size: 54 1425 50 275 18 583 630 889
Cuts at: 3932 5561 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 630 583 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3043 1537
Size: 275 1231 4090
Fragments arranged by size:
4090 1231 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2290 2536 3526 1353
Size: 937 246 990 3423
Fragments arranged by size:
3423 990 937 246
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4183 4557 4703 387
Size: 1064 138 2594 374 146 1280
Fragments arranged by size:
2594 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2361 2503 2559 3659 4256 5043 5311
Size: 393 729 142 56 1100 597 787 268
Cuts at: 5311 1239
Size: 1524
Fragments arranged by size:
1524 1100 787 729 597 393 268 142
56
EcoO109I rG'GnC_Cy
Cuts at: 2102 2103 2547 2102
Size: 1 444 5151
Fragments arranged by size:
5151 444 1
EcoRI G'AATT_C
Cuts at: 2905 3283 2905
Size: 378 5218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2851 2851
Size: 5596
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 2146 4743 1678
Size: 468 2597 2531
Fragments arranged by size:
2597 2531 468
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2149 2181 2504
Size: 32 135 32 427 32 118 32 323
Cuts at: 2504 2536 2551 2568 2583 2618 2650 2855 2887
Size: 32 15 17 15 35 32 205 32
Cuts at: 2887 4237 4269 4311 4343 1373
Size: 1350 32 42 32 2626
Fragments arranged by size:
2626 1350 427 323 205 135 118 42
35 32 32 32 32 32 32 32
32 32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2940 3186 669
Size: 988 281 1002 246 3079
Fragments arranged by size:
3079 1002 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2767 2828 748
Size: 2019 61 3516
Fragments arranged by size:
3516 2019 61
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KpnI G_GTAC'C
Cuts at: 2215 2700 2921 2215
Size: 485 221 4890
Fragments arranged by size:
4890 485 221
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4710 4894 1305
Size: 3405 184 2007
Fragments arranged by size:
3405 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2415 10
Size: 54 1425 343 583 3191
Fragments arranged by size:
3191 1425 583 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 2875 3554 3913 4072 518
Size: 648 1059 650 679 359 159 2042
Fragments arranged by size:
2042 1059 679 650 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3012 3689 4837 5082 1606
Size: 1406 677 1148 245 2120
Fragments arranged by size:
2120 1406 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2691 513
Size: 552 1626 3418
Fragments arranged by size:
3418 1626 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NsiI A_TGCA'T
Cuts at: 2655 2655
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2542 2954 4499 1132
Size: 97 1313 412 1545 2229
Fragments arranged by size:
2229 1545 1313 412 97
PasI CC'CwG_GG
Cuts at: 1348 2106 2749 1348
Size: 758 643 4195
Fragments arranged by size:
4195 758 643
PciI A'CATG_T
Cuts at: 4495 4495
Size: 5596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2893 1108
Size: 1785 3811
PfoI T'CCnGG_A
Cuts at: 2665 2822 2665
Size: 157 5439
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 5178 5210 3665
Size: 32 1481 32 4051
Fragments arranged by size:
4051 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2547 2547
Size: 5596
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5596
PsiI TTA'TAA
Cuts at: 2805 2859 3166 2805
Size: 54 307 5235
Fragments arranged by size:
5235 307 54
PspOMI G'GGCC_C
Cuts at: 2102 2102
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2948 830
Size: 2118 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SapI GCTCTTCn'nnn_
Cuts at: 2290 2290
Size: 5596
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 3844 5556 3844
Size: 1712 3884
SfcI C'TryA_G
Cuts at: 826 1045 2944 4079 4760 4951 826
Size: 219 1899 1135 681 191 1471
Fragments arranged by size:
1899 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2100 2923 1872
Size: 228 823 4545
Fragments arranged by size:
4545 823 228
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2018 3700 4601 4863 820
Size: 197 926 75 1682 901 262 1553
Fragments arranged by size:
1682 1553 926 901 262 197 75
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2542 2954 1132
Size: 1410 412 3774
Fragments arranged by size:
3774 1410 412
SspI AAT'ATT
Cuts at: 5 52 3520 5
Size: 47 3468 2081
Fragments arranged by size:
3468 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5596
StyI C'CwwG_G
Cuts at: 513 1065 2691 3035 513
Size: 552 1626 344 3074
Fragments arranged by size:
3074 1626 552 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2762 3623 3640 3793 3978 4423 325
Size: 1255 1182 861 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1182 861 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3842 5554 96
Size: 274 80 33 51 1443 1865 1712 138
Fragments arranged by size:
1865 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2671 2703 5556 5588 2671
Size: 32 2853 32 2679
Fragments arranged by size:
2853 2679 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2065 2794 2065
Size: 729 4867
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2098 2921 1870
Size: 228 823 4545
Fragments arranged by size:
4545 823 228
XmnI GAAnn'nnTTC
Cuts at: 3725 3725
Size: 5596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI