Restriction map for
pFN21AB7602
(Circular) (Six-base) MAPSORT of: pFN21AB7602.seq Check: 8494 from: 1 to: 5767
pFN21AB7602 5767 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3110 1937
Size: 1173 4594
Acc65I G'GTAC_C
Cuts at: 3088 3088
Size: 5767
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3830 5214 5482 1632
Size: 2198 1384 268 1917
Fragments arranged by size:
2198 1917 1384 268
AfeI AGC'GCT
Cuts at: 2627 2627
Size: 5767
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 2141 4666 5643 1203
Size: 938 2525 977 1327
Fragments arranged by size:
2525 1327 977 938
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5767
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5767
AlwNI CAG_nnn'CTG
Cuts at: 2456 2465 3510 5082 2456
Size: 9 1045 1572 3141
Fragments arranged by size:
3141 1572 1045 9
ApaI G_GGCC'C
Cuts at: 2064 2064
Size: 5767
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 3076 3302 3454 5563 1493
Size: 1583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 2162 2359 3092 1870
Size: 73 219 197 733 4545
Fragments arranged by size:
4545 733 219 197 73
BamHI G'GATC_C
Cuts at: 3097 3464 3097
Size: 367 5400
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2581 2642 2806 2972 3088
Size: 325 217 627 794 61 164 166 116
Cuts at: 3088 4543 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 794 627 325 217 166 164
116 61
BanII G_rGCy'C
Cuts at: 1391 2064 2360 2366 2540 3086 1391
Size: 673 296 6 174 546 4072
Fragments arranged by size:
4072 673 546 296 174 6
BbeI G_GCGC'C
Cuts at: 2646 2976 2646
Size: 330 5437
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4796
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3958 3992 1903
Size: 34 2021 34 3678
Fragments arranged by size:
3678 2021 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3659 4869 3659
Size: 1210 4557
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2688 4378 136
Size: 107 122 71 631 605 1016 1690 1525
Fragments arranged by size:
1690 1525 1016 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5762 5762
Size: 5767
BlpI GC'TnA_GC
Cuts at: 2560 2795 3184 2560
Size: 235 389 5143
Fragments arranged by size:
5143 389 235
Bme1580I G_kGCm'C
Cuts at: 1790 2064 3828 4984 1790
Size: 274 1764 1156 2573
Fragments arranged by size:
2573 1764 1156 274
BmgBI CAC'GTC
Cuts at: 1318 2158 1318
Size: 840 4927
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3498 4456 442
Size: 416 53 83 399 24 2081 958 1753
Fragments arranged by size:
2081 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2328 2554 4427 1239
Size: 1089 226 1873 2579
Fragments arranged by size:
2579 1873 1089 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2207 3892 4757 5055 2207
Size: 1685 865 298 2919
Fragments arranged by size:
2919 1685 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4430 882
Size: 617 2931 2219
Fragments arranged by size:
2931 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3558 493
Size: 711 2354 2702
Fragments arranged by size:
2702 2354 711
BsaBI GATnn'nnATC
Cuts at: 5761 5761
Size: 5767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2429 2643 2973 3574
Size: 53 83 186 936 896 214 330 601
Cuts at: 3574 3956 4591 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 896 635 601 382 330 214
186 83 53
BsaWI w'CCGG_w
Cuts at: 4200 4598 4872 5019 5430 4200
Size: 398 274 147 411 4537
Fragments arranged by size:
4537 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2996 3026 1178
Size: 30 165 30 1593 30 3919
Fragments arranged by size:
3919 1593 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2225 2710 2803 1186
Size: 1039 485 93 4150
Fragments arranged by size:
4150 1039 485 93
BseYI C'CCAG_C
Cuts at: 2047 2323 2449 4970 5466 2047
Size: 276 126 2521 496 2348
Fragments arranged by size:
2521 2348 496 276 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2063 1163
Size: 573 327 4867
Fragments arranged by size:
4867 573 327
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2496 3217 3978 5006 1540
Size: 275 186 495 721 761 1028 2301
Fragments arranged by size:
2301 1028 761 721 495 275 186
BsiHKAI G_wGCw'C
Cuts at: 2163 2360 3828 3913 4984 2163
Size: 197 1468 85 1071 2946
Fragments arranged by size:
2946 1468 1071 197 85
BsmI GAATG_Cn'
Cuts at: 3278 3371 3278
Size: 93 5674
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2163 2360 2366 2540 3086 3828
Size: 399 274 99 197 6 174 546 742
Cuts at: 3828 3913 4984 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 546 399 274 197 174
99 85 6
BspEI T'CCGG_A
Cuts at: 5430 5430
Size: 5767
BspHI T'CATG_A
Cuts at: 3656 5386 3656
Size: 1730 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2241 3122 844
Size: 1397 881 3489
Fragments arranged by size:
3489 1397 881
BsrBI CCG'CTC
Cuts at: 1394 1823 2301 2307 3654 1394
Size: 429 478 6 1347 3507
Fragments arranged by size:
3507 1347 478 429 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 4262 4436 65
Size: 1183 3014 174 1396
Fragments arranged by size:
3014 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4411 4598 1700
Size: 110 2601 187 2869
Fragments arranged by size:
2869 2601 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5767
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5767
BssSI C'ACGA_G
Cuts at: 2614 3827 4839 2614
Size: 1213 1012 3542
Fragments arranged by size:
3542 1213 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2465 794
Size: 1671 4096
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 3097 3464 3849 3866 5318 5404 5416 5762
Size: 1125 367 385 17 1452 86 12 346
Cuts at: 5762 1972
Size: 1977
Fragments arranged by size:
1977 1452 1125 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2872 2872
Size: 5767
BtgI C'CryG_G
Cuts at: 513 1065 2067 2088 2108 2802 2817 513
Size: 552 1002 21 20 694 15 3463
Fragments arranged by size:
3463 1002 694 552 21 20 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5028
Fragments arranged by size:
5028 683 56
BtsI GCAGTG_nn'
Cuts at: 2380 3283 4077 4097 2380
Size: 903 794 20 4050
Fragments arranged by size:
4050 903 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DraIII CAC_nnn'GTG
Cuts at: 2616 2616
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4774 809
Size: 507 3458 1802
Fragments arranged by size:
3458 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2100 2377
Size: 54 1425 50 275 18 225 45 277
Cuts at: 2377 2493 2595 2680 2698 3214 4103 5732 8
Size: 116 102 85 18 516 889 1629 43
Fragments arranged by size:
1629 1425 889 516 277 275 225 116
102 85 54 50 45 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2493 3214 1537
Size: 275 681 721 4090
Fragments arranged by size:
4090 721 681 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2177 2443 2728 3697 1353
Size: 824 266 285 969 3423
Fragments arranged by size:
3423 969 824 285 266
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2431 2980 4354 4728 4874 387
Size: 1064 138 842 549 1374 374 146 1280
Fragments arranged by size:
1374 1280 1064 842 549 374 146 138
EcoICRI GAG'CTC
Cuts at: 2358 2358
Size: 5767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2328 2554 3830 4427 5214 5482 1239
Size: 393 696 226 1276 597 787 268 1524
Fragments arranged by size:
1524 1276 787 696 597 393 268 226
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
FspI TGC'GCA
Cuts at: 2850 4273 2850
Size: 1423 4344
FspAI rTGC'GCAy
Cuts at: 2850 2850
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 2171 2222 2567 2629 2646 2765 2976 4914
Size: 493 51 345 62 17 119 211 1938
Cuts at: 4914 1678
Size: 2531
Fragments arranged by size:
2531 1938 493 345 211 119 62 51
17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2238 2270 2735 2767 3029
Size: 32 135 32 666 32 465 32 262
Cuts at: 3029 3061 4408 4440 4482 4514 1373
Size: 32 1347 32 42 32 2626
Fragments arranged by size:
2626 1347 666 465 262 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KasI G'GCGC_C
Cuts at: 2642 2972 2642
Size: 330 5437
KpnI G_GTAC'C
Cuts at: 3092 3092
Size: 5767
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 2141 5643 2141
Size: 3502 2265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4881 5065 1305
Size: 3576 184 2007
Fragments arranged by size:
3576 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2379 10
Size: 54 1425 343 547 3398
Fragments arranged by size:
3398 1425 547 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2844 3725 4084 4243 518
Size: 648 1035 643 881 359 159 2042
Fragments arranged by size:
2042 1035 881 648 643 359 159
MspA1I CmG'CkG
Cuts at: 1606 2110 2119 2152 2396 2453 2462 2519 2978
Size: 504 9 33 244 57 9 57 459
Cuts at: 2978 3183 3860 5008 5253 1606
Size: 205 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 504 459 245 244 205
57 57 33 9 9
NarI GG'CG_CC
Cuts at: 2643 2973 2643
Size: 330 5437
NcoI C'CATG_G
Cuts at: 513 1065 2067 2802 2817 513
Size: 552 1002 735 15 3463
Fragments arranged by size:
3463 1002 735 552 15
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5767
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 2493 3214 2493
Size: 721 5046
NspI r_CATG'y
Cuts at: 1132 1229 2849 3125 4670 1132
Size: 97 1620 276 1545 2229
Fragments arranged by size:
2229 1620 1545 276 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5767
PciI A'CATG_T
Cuts at: 4666 4666
Size: 5767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3064 1108
Size: 1956 3811
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 5349 5381 3836
Size: 32 1481 32 4222
Fragments arranged by size:
4222 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PspOMI G'GGCC_C
Cuts at: 2060 2060
Size: 5767
PspXI vC'TCGA_Gb
Cuts at: 2162 2162
Size: 5767
PstI C_TGCA'G
Cuts at: 830 2461 2641 2750 3119 830
Size: 1631 180 109 369 3478
Fragments arranged by size:
3478 1631 369 180 109
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
PvuII CAG'CTG
Cuts at: 2152 2396 2519 2152
Size: 244 123 5400
Fragments arranged by size:
5400 244 123
SacI G_AGCT'C
Cuts at: 2360 2360
Size: 5767
SacII CC_GC'GG
Cuts at: 2111 2111
Size: 5767
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SapI GCTCTTCn'nnn_
Cuts at: 2177 2443 2177
Size: 266 5501
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SfcI C'TryA_G
Cuts at: 826 1045 2457 2637 2746 3115 4250 4931 5122
Size: 219 1412 180 109 369 1135 681 191
Cuts at: 5122 826
Size: 1471
Fragments arranged by size:
1471 1412 1135 681 369 219 191 180
109
SfoI GGC'GCC
Cuts at: 2644 2974 2644
Size: 330 5437
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 3094 1872
Size: 1222 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2162 2222 3871 4772 5034 820
Size: 197 926 219 60 1649 901 262 1553
Fragments arranged by size:
1649 1553 926 901 262 219 197 60
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 3125 1132
Size: 1993 3774
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5767
StyI C'CwwG_G
Cuts at: 513 1065 2067 2701 2802 2817 2908 3206 513
Size: 552 1002 634 101 15 91 298 3074
Fragments arranged by size:
3074 1002 634 552 298 101 91 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2097 2856 3794 3811 3964 4149 4594
Size: 1255 517 759 938 17 153 185 445
Cuts at: 4594 325
Size: 1498
Fragments arranged by size:
1498 1255 938 759 517 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2510 4013 5725
Size: 274 80 33 51 1443 533 1503 1712
Cuts at: 5725 96
Size: 138
Fragments arranged by size:
1712 1503 1443 533 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5727 5759 5727
Size: 32 5735
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 2243 3103 2243
Size: 860 4907
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2915 2915
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 2162 1943
Size: 219 5548
XmaI C'CCGG_G
Cuts at: 1870 3092 1870
Size: 1222 4545
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I EcoRV
FalI FseI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PsrI RsrII SanDI SexAI SfiI SrfI
SwaI