Restriction map for
pFN21AB7601
(Circular) (Six-base) MAPSORT of: pFN21AB7601.seq Check: 631 from: 1 to: 5581
pFN21AB7601 5581 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2527 2541 2564 2642 2527
Size: 14 23 78 5466
Fragments arranged by size:
5466 78 23 14
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3391 278
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2283 2924 1937
Size: 346 641 4594
Fragments arranged by size:
4594 641 346
Acc65I G'GTAC_C
Cuts at: 2902 2902
Size: 5581
AclI AA'CG_TT
Cuts at: 1563 3708 4081 1563
Size: 2145 373 3063
Fragments arranged by size:
3063 2145 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3644 5028 5296 1632
Size: 2012 1384 268 1917
Fragments arranged by size:
2012 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5384
AflIII A'CryG_T
Cuts at: 1203 4480 5457 1203
Size: 3277 977 1327
Fragments arranged by size:
3277 1327 977
AgeI A'CCGG_T
Cuts at: 2799 4412 2799
Size: 1613 3968
AhdI GACnn_n'nnGTC
Cuts at: 4310 4310
Size: 5581
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5581
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2228 1184
Size: 1044 4537
AlwNI CAG_nnn'CTG
Cuts at: 2667 3324 4896 2667
Size: 657 1572 3352
Fragments arranged by size:
3352 1572 657
ApaI G_GGCC'C
Cuts at: 2393 2767 2879 2393
Size: 374 112 5095
Fragments arranged by size:
5095 374 112
ApaLI G'TGCA_C
Cuts at: 3638 4794 3638
Size: 1156 4425
ApoI r'AATT_y
Cuts at: 1493 2890 3116 3268 5377 1493
Size: 1397 226 152 2109 1697
Fragments arranged by size:
2109 1697 1397 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4135 160
Size: 872 3103 1606
Fragments arranged by size:
3103 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
AvaI C'yCGr_G
Cuts at: 1870 1943 2133 2289 2442 2449 2872 2906 1870
Size: 73 190 156 153 7 423 34 4545
Fragments arranged by size:
4545 423 190 156 153 73 34 7
BamHI G'GATC_C
Cuts at: 2911 3278 2911
Size: 367 5214
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2119 2511 2591 2802
Size: 325 217 627 303 29 392 80 211
Cuts at: 2802 2902 4357 618
Size: 100 1455 1842
Fragments arranged by size:
1842 1455 627 392 325 303 217 211
100 80 29
BanII G_rGCy'C
Cuts at: 1391 2393 2410 2449 2767 2879 2900 1391
Size: 1002 17 39 318 112 21 4072
Fragments arranged by size:
4072 1002 318 112 39 21 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2542 928
Size: 971 643 3967
Fragments arranged by size:
3967 971 643
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3772 3806 1903
Size: 34 50 34 1751 34 3678
Fragments arranged by size:
3678 1751 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3473 4683 3473
Size: 1210 4371
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5581
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2300 2504 4192
Size: 107 122 71 631 605 628 204 1688
Cuts at: 4192 136
Size: 1525
Fragments arranged by size:
1688 1525 631 628 605 204 122 107
71
BglII A'GATC_T
Cuts at: 2640 5576 2640
Size: 2936 2645
BlpI GC'TnA_GC
Cuts at: 2430 2998 2430
Size: 568 5013
Bme1580I G_kGCm'C
Cuts at: 1790 2124 2160 2393 2596 2767 2879 3642 4798
Size: 334 36 233 203 171 112 763 1156
Cuts at: 4798 1790
Size: 2573
Fragments arranged by size:
2573 1156 763 334 233 203 171 112
36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5581
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3312 4270 442
Size: 416 53 83 399 24 1895 958 1753
Fragments arranged by size:
1895 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2020 2087 2644 4241 1239
Size: 781 67 557 1597 2579
Fragments arranged by size:
2579 1597 781 557 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2582 3706 4571 4869 2582
Size: 1124 865 298 3294
Fragments arranged by size:
3294 1124 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4244 882
Size: 617 2745 2219
Fragments arranged by size:
2745 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3372 493
Size: 711 2168 2702
Fragments arranged by size:
2702 2168 711
BsaBI GATnn'nnATC
Cuts at: 2606 5575 2606
Size: 2969 2612
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2262 2787 3388 3770
Size: 53 83 186 936 729 525 601 382
Cuts at: 3770 4405 275
Size: 635 1451
Fragments arranged by size:
1451 936 729 635 601 525 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2410 2799 4014 4412 4686 4833 5244 2410
Size: 389 1215 398 274 147 411 2747
Fragments arranged by size:
2747 1215 411 398 389 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5356
Fragments arranged by size:
5356 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2179 2870 1186
Size: 931 62 691 3897
Fragments arranged by size:
3897 931 691 62
BseYI C'CCAG_C
Cuts at: 2172 4784 5280 2172
Size: 2612 496 2473
Fragments arranged by size:
2612 2473 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2507 2614 2797 1163
Size: 573 771 107 183 3947
Fragments arranged by size:
3947 771 573 183 107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2196 2749 3031 3792 4820 1540
Size: 275 186 195 553 282 761 1028 2301
Fragments arranged by size:
2301 1028 761 553 282 275 195 186
BsiHKAI G_wGCw'C
Cuts at: 3642 3727 4798 3642
Size: 85 1071 4425
Fragments arranged by size:
4425 1071 85
BsmI GAATG_Cn'
Cuts at: 3092 3185 3092
Size: 93 5488
BsmBI CGTCTCn'nnnn_
Cuts at: 2063 2063
Size: 5581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2160 2393 2410 2449 2596 2767
Size: 399 334 36 233 17 39 147 171
Cuts at: 2767 2879 2900 3642 3727 4798 1391
Size: 112 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 334 233 171 147
112 85 39 36 21 17
BspEI T'CCGG_A
Cuts at: 2410 5244 2410
Size: 2834 2747
BspHI T'CATG_A
Cuts at: 3470 5200 3470
Size: 1730 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2352 2527 2541 2564 2642 2936 844
Size: 1508 175 14 23 78 294 3489
Fragments arranged by size:
3489 1508 294 175 78 23 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2187 2434 2751 3468 1394
Size: 429 364 247 317 717 3507
Fragments arranged by size:
3507 717 429 364 317 247
BsrDI GCAATG_nn'
Cuts at: 65 1248 4076 4250 65
Size: 1183 2828 174 1396
Fragments arranged by size:
2828 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2058 2397 2514 2799 2805 4225 4412
Size: 110 248 339 117 285 6 1420 187
Cuts at: 4412 1700
Size: 2869
Fragments arranged by size:
2869 1420 339 285 248 187 117 110
6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5581
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5581
BssSI C'ACGA_G
Cuts at: 3641 4653 3641
Size: 1012 4569
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2565 794
Size: 1771 3810
BstBI TT'CG_AA
Cuts at: 4385 4385
Size: 5581
BstEII G'GTnAC_C
Cuts at: 1184 2162 1184
Size: 978 4603
BstYI r'GATC_y
Cuts at: 1972 2640 2911 3278 3663 3680 5132 5218 5230
Size: 668 271 367 385 17 1452 86 12
Cuts at: 5230 5576 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 668 385 367 346 271 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2225 513
Size: 552 1160 3869
Fragments arranged by size:
3869 1160 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4842
Fragments arranged by size:
4842 683 56
BtsI GCAGTG_nn'
Cuts at: 2351 2470 2524 2725 3097 3891 3911 2351
Size: 119 54 201 372 794 20 4021
Fragments arranged by size:
4021 794 372 201 119 54 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5581
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2626 2661 529
Size: 35 2062 35 3449
Fragments arranged by size:
3449 2062 35 35
DraI TTT'AAA
Cuts at: 2885 2885
Size: 5581
DraIII CAC_nnn'GTG
Cuts at: 2373 2373
Size: 5581
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4588 809
Size: 507 3272 1802
Fragments arranged by size:
3272 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2496 2505
Size: 54 1425 50 275 18 363 303 9
Cuts at: 2505 3028 3917 5546 8
Size: 523 889 1629 43
Fragments arranged by size:
1629 1425 889 523 363 303 275 54
50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2193 3028 1537
Size: 275 381 835 4090
Fragments arranged by size:
4090 835 381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3511 1353
Size: 2158 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4168 4542 4688 387
Size: 1064 138 2579 374 146 1280
Fragments arranged by size:
2579 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2087 2644 3644 4241 5028 5296
Size: 393 388 67 557 1000 597 787 268
Cuts at: 5296 1239
Size: 1524
Fragments arranged by size:
1524 1000 787 597 557 393 388 268
67
EcoNI CCTnn'n_nnAGG
Cuts at: 2659 2659
Size: 5581
EcoO109I rG'GnC_Cy
Cuts at: 2047 2146 2247 2664 2736 2876 2047
Size: 99 101 417 72 140 4752
Fragments arranged by size:
4752 417 140 101 99 72
EcoRI G'AATT_C
Cuts at: 2890 3268 2890
Size: 378 5203
FspI TGC'GCA
Cuts at: 4087 4087
Size: 5581
HaeII r_GCGC'y
Cuts at: 1678 2812 4728 1678
Size: 1134 1916 2531
Fragments arranged by size:
2531 1916 1134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2174 2206 4222 4254 4296
Size: 32 135 32 602 32 2016 32 42
Cuts at: 4296 4328 1373
Size: 32 2626
Fragments arranged by size:
2626 2016 602 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2284 2925 3171 669
Size: 988 281 346 641 246 3079
Fragments arranged by size:
3079 988 641 346 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5581
HpaI GTT'AAC
Cuts at: 3171 3171
Size: 5581
KpnI G_GTAC'C
Cuts at: 2906 2906
Size: 5581
MfeI C'AATT_G
Cuts at: 3180 3180
Size: 5581
MluI A'CGCG_T
Cuts at: 5457 5457
Size: 5581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2778 4695 4879 1305
Size: 1473 1917 184 2007
Fragments arranged by size:
2007 1917 1473 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2507 10
Size: 54 1425 343 675 3084
Fragments arranged by size:
3084 1425 675 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3539 3898 4057 518
Size: 648 2373 359 159 2042
Fragments arranged by size:
2373 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2521 2997 3674 4822 5067 1606
Size: 915 476 677 1148 245 2120
Fragments arranged by size:
2120 1148 915 677 476 245
NaeI GCC'GGC
Cuts at: 2807 2807
Size: 5581
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5029
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5581
NgoMIV G'CCGG_C
Cuts at: 2805 2805
Size: 5581
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5581
NotI GC'GGCC_GC
Cuts at: 3028 3028
Size: 5581
NspI r_CATG'y
Cuts at: 1132 1229 2836 2939 4484 1132
Size: 97 1607 103 1545 2229
Fragments arranged by size:
2229 1607 1545 103 97
PasI CC'CwG_GG
Cuts at: 1348 2178 1348
Size: 830 4751
PciI A'CATG_T
Cuts at: 4480 4480
Size: 5581
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2373 2494 2667 1108
Size: 1265 121 173 4022
Fragments arranged by size:
4022 1265 173 121
PmeI GTTT'AAAC
Cuts at: 2885 2885
Size: 5581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3650 3682 5163 5195 3650
Size: 32 1481 32 4036
Fragments arranged by size:
4036 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 2047
Size: 5581
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5581
PsiI TTA'TAA
Cuts at: 3151 3151
Size: 5581
PspOMI G'GGCC_C
Cuts at: 2389 2763 2875 2389
Size: 374 112 5095
Fragments arranged by size:
5095 374 112
PstI C_TGCA'G
Cuts at: 830 2538 2679 2732 2933 830
Size: 1708 141 53 201 3478
Fragments arranged by size:
3478 1708 201 141 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5581
PvuII CAG'CTG
Cuts at: 2521 2521
Size: 5581
SalI G'TCGA_C
Cuts at: 1936 2282 2923 1936
Size: 346 641 4594
Fragments arranged by size:
4594 641 346
SanDI GG'GwC_CC
Cuts at: 2047 2047
Size: 5581
SbfI CC_TGCA'GG
Cuts at: 2538 2933 2538
Size: 395 5186
ScaI AGT'ACT
Cuts at: 2564 3829 5541 2564
Size: 1265 1712 2604
Fragments arranged by size:
2604 1712 1265
SfcI C'TryA_G
Cuts at: 826 1045 2534 2675 2728 2929 4064 4745 4936
Size: 219 1489 141 53 201 1135 681 191
Cuts at: 4936 826
Size: 1471
Fragments arranged by size:
1489 1471 1135 681 219 201 191 141
53
SfiI GGCCn_nnn'nGGCC
Cuts at: 2300 2504 2300
Size: 204 5377
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5581
SmaI CCC'GGG
Cuts at: 1872 2451 2874 2908 1872
Size: 579 423 34 4545
Fragments arranged by size:
4545 579 423 34
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2597 3685 4586 4848 820
Size: 197 926 654 1088 901 262 1553
Fragments arranged by size:
1553 1088 926 901 654 262 197
SnaBI TAC'GTA
Cuts at: 493 3372 493
Size: 2879 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5581
SphI G_CATG'C
Cuts at: 1132 2939 1132
Size: 1807 3774
SrfI GCCC'GGGC
Cuts at: 2874 2874
Size: 5581
SspI AAT'ATT
Cuts at: 5 52 3505 5
Size: 47 3453 2081
Fragments arranged by size:
3453 2081 47
StuI AGG'CCT
Cuts at: 1517 2654 1517
Size: 1137 4444
StyI C'CwwG_G
Cuts at: 513 1065 2265 2385 3020 513
Size: 552 1200 120 635 3074
Fragments arranged by size:
3074 1200 635 552 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2579 2708 3608 3625 3778 3963 4408
Size: 1255 999 129 900 17 153 185 445
Cuts at: 4408 325
Size: 1498
Fragments arranged by size:
1498 1255 999 900 445 185 153 129
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2562 2577 3827
Size: 274 80 33 51 1443 585 15 1250
Cuts at: 3827 5539 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1250 585 274 138 80 51
33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5541 5573 5541
Size: 32 5549
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5581
XbaI T'CTAG_A
Cuts at: 2917 2917
Size: 5581
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5581
XmaI C'CCGG_G
Cuts at: 1870 2449 2872 2906 1870
Size: 579 423 34 4545
Fragments arranged by size:
4545 579 423 34
XmnI GAAnn'nnTTC
Cuts at: 3710 3710
Size: 5581
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3389 276
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SapI
SexAI SfoI SwaI XcmI