Restriction map for
pFN21AB7600
(Circular) (Six-base) MAPSORT of: pFN21AB7600.seq Check: 8390 from: 1 to: 6007
pFN21AB7600 6007 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2126 2622 2126
Size: 496 5511
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2673 3817 278
Size: 53 83 186 936 1137 1144 2468
Fragments arranged by size:
2468 1144 1137 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2571 3350 1937
Size: 634 779 4594
Fragments arranged by size:
4594 779 634
Acc65I G'GTAC_C
Cuts at: 3328 3328
Size: 6007
AclI AA'CG_TT
Cuts at: 1563 4134 4507 1563
Size: 2571 373 3063
Fragments arranged by size:
3063 2571 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4070 5454 5722 1632
Size: 2438 1384 268 1917
Fragments arranged by size:
2438 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5810
AflIII A'CryG_T
Cuts at: 1203 4906 5883 1203
Size: 3703 977 1327
Fragments arranged by size:
3703 1327 977
AgeI A'CCGG_T
Cuts at: 2059 4838 2059
Size: 2779 3228
AhdI GACnn_n'nnGTC
Cuts at: 2128 2674 4736 2128
Size: 546 2062 3399
Fragments arranged by size:
3399 2062 546
AleI CACnn'nnGTG
Cuts at: 1166 2042 3065 1166
Size: 876 1023 4108
Fragments arranged by size:
4108 1023 876
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6007
AlwNI CAG_nnn'CTG
Cuts at: 2027 2872 3013 3148 3750 5322 2027
Size: 845 141 135 602 1572 2712
Fragments arranged by size:
2712 1572 845 602 141 135
ApaI G_GGCC'C
Cuts at: 2401 2939 2401
Size: 538 5469
ApaLI G'TGCA_C
Cuts at: 4064 5220 4064
Size: 1156 4851
ApoI r'AATT_y
Cuts at: 1493 3316 3542 3694 5803 1493
Size: 1823 226 152 2109 1697
Fragments arranged by size:
2109 1823 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4561 160
Size: 872 3529 1606
Fragments arranged by size:
3529 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
AvaI C'yCGr_G
Cuts at: 1870 1943 2939 2976 3332 1870
Size: 73 996 37 356 4545
Fragments arranged by size:
4545 996 356 73 37
BamHI G'GATC_C
Cuts at: 3337 3704 3337
Size: 367 5640
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3328 4783 618
Size: 325 217 627 1541 1455 1842
Fragments arranged by size:
1842 1541 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2239 2265 2401 2540 2726 2939 3326 1391
Size: 848 26 136 139 186 213 387 4072
Fragments arranged by size:
4072 848 387 213 186 139 136 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5036
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4198 4232 1903
Size: 34 2261 34 3678
Fragments arranged by size:
3678 2261 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3899 5109 3899
Size: 1210 4797
BclI T'GATC_A
Cuts at: 1548 2106 2863 1548
Size: 558 757 4692
Fragments arranged by size:
4692 757 558
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2018 2636 3200
Size: 107 122 71 631 605 346 618 564
Cuts at: 3200 4618 136
Size: 1418 1525
Fragments arranged by size:
1525 1418 631 618 605 564 346 122
107 71
BglII A'GATC_T
Cuts at: 6002 6002
Size: 6007
BlpI GC'TnA_GC
Cuts at: 2238 2909 3424 2238
Size: 671 515 4821
Fragments arranged by size:
4821 671 515
Bme1580I G_kGCm'C
Cuts at: 1790 2401 2800 2939 4068 5224 1790
Size: 611 399 139 1129 1156 2573
Fragments arranged by size:
2573 1156 1129 611 399 139
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2067 3738 4696
Size: 416 53 83 399 24 650 1671 958
Cuts at: 4696 442
Size: 1753
Fragments arranged by size:
1753 1671 958 650 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2279 2915 3095 4667 1239
Size: 1040 636 180 1572 2579
Fragments arranged by size:
2579 1572 1040 636 180
Bpu10I CC'TnA_GC
Cuts at: 2831 2831
Size: 6007
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4132 4997 5295 4132
Size: 865 298 4844
Fragments arranged by size:
4844 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4670 882
Size: 617 3171 2219
Fragments arranged by size:
3171 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3249 3798 493
Size: 711 2045 549 2702
Fragments arranged by size:
2702 2045 711 549
BsaBI GATnn'nnATC
Cuts at: 6001 6001
Size: 6007
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2520 2670 3814 4196
Size: 53 83 186 936 987 150 1144 382
Cuts at: 4196 4831 275
Size: 635 1451
Fragments arranged by size:
1451 1144 987 936 635 382 186 150
83 53
BsaWI w'CCGG_w
Cuts at: 2059 4440 4838 5112 5259 5670 2059
Size: 2381 398 274 147 411 2396
Fragments arranged by size:
2396 2381 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2363 2393 1178
Size: 30 165 30 960 30 4792
Fragments arranged by size:
4792 960 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2330 2609 3311 1186
Size: 1144 279 702 3882
Fragments arranged by size:
3882 1144 702 279
BseYI C'CCAG_C
Cuts at: 2581 2767 3006 5210 5706 2581
Size: 186 239 2204 496 2882
Fragments arranged by size:
2882 2204 496 239 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2498 2702 2879 3101 1163
Size: 573 762 204 177 222 4069
Fragments arranged by size:
4069 762 573 222 204 177
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3457 4218 5246 1540
Size: 275 186 1456 761 1028 2301
Fragments arranged by size:
2301 1456 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2763 4068 4153 5224 2763
Size: 1305 85 1071 3546
Fragments arranged by size:
3546 1305 1071 85
BsiWI C'GTAC_G
Cuts at: 2713 2713
Size: 6007
BsmI GAATG_Cn'
Cuts at: 3045 3159 3518 3611 3045
Size: 114 359 93 5441
Fragments arranged by size:
5441 359 114 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2239 2265 2401 2540 2726 2763 2800
Size: 399 449 26 136 139 186 37 37
Cuts at: 2800 2939 3326 4068 4153 5224 1391
Size: 139 387 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 449 399 387 186 139
139 136 85 37 37 26
BspEI T'CCGG_A
Cuts at: 5670 5670
Size: 6007
BspHI T'CATG_A
Cuts at: 3896 5626 3896
Size: 1730 4277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2126 2622 2956 3362 844
Size: 1282 496 334 406 3489
Fragments arranged by size:
3489 1282 496 406 334
BsrBI CCG'CTC
Cuts at: 1394 1823 2484 3894 1394
Size: 429 661 1410 3507
Fragments arranged by size:
3507 1410 661 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3000 4502 4676 65
Size: 1183 1752 1502 174 1396
Fragments arranged by size:
1752 1502 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2059 2513 4651 4838 1700
Size: 110 249 454 2138 187 2869
Fragments arranged by size:
2869 2138 454 249 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6007
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6007
BssSI C'ACGA_G
Cuts at: 3254 4067 5079 3254
Size: 813 1012 4182
Fragments arranged by size:
4182 1012 813
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2141 794
Size: 1347 4660
BstBI TT'CG_AA
Cuts at: 4811 4811
Size: 6007
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6007
BstXI CCAn_nnnn'nTGG
Cuts at: 2080 2656 2080
Size: 576 5431
BstYI r'GATC_y
Cuts at: 1972 3337 3704 4089 4106 5558 5644 5656 6002
Size: 1365 367 385 17 1452 86 12 346
Cuts at: 6002 1972
Size: 1977
Fragments arranged by size:
1977 1452 1365 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2343 2467 513
Size: 552 1278 124 4053
Fragments arranged by size:
4053 1278 552 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2629 487
Size: 683 56 1403 3865
Fragments arranged by size:
3865 1403 683 56
BtsI GCAGTG_nn'
Cuts at: 3523 4317 4337 3523
Size: 794 20 5193
Fragments arranged by size:
5193 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5972
DraI TTT'AAA
Cuts at: 3311 3311
Size: 6007
DraIII CAC_nnn'GTG
Cuts at: 3217 3217
Size: 6007
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5014 809
Size: 507 3698 1802
Fragments arranged by size:
3698 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2433 3454
Size: 54 1425 50 275 18 261 342 1021
Cuts at: 3454 4343 5972 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1021 889 342 275 261 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3454 1537
Size: 275 1642 4090
Fragments arranged by size:
4090 1642 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3937 1353
Size: 2584 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2375 2618 4594 4968 5114 387
Size: 1064 138 786 243 1976 374 146 1280
Fragments arranged by size:
1976 1280 1064 786 374 243 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2279 2915 3095 4070 4667 5454 5722
Size: 393 647 636 180 975 597 787 268
Cuts at: 5722 1239
Size: 1524
Fragments arranged by size:
1524 975 787 647 636 597 393 268
180
EcoO109I rG'GnC_Cy
Cuts at: 2208 2353 2397 2827 2935 2936 3052 3106 3145
Size: 145 44 430 108 1 116 54 39
Cuts at: 3145 3202 3260 2208
Size: 57 58 4955
Fragments arranged by size:
4955 430 145 116 108 58 57 54
44 39 1
EcoRI G'AATT_C
Cuts at: 3316 3694 3316
Size: 378 5629
FspI TGC'GCA
Cuts at: 4513 4513
Size: 6007
HaeII r_GCGC'y
Cuts at: 1678 2421 2984 3195 5154 1678
Size: 743 563 211 1959 2531
Fragments arranged by size:
2531 1959 743 563 211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 2363 2395 2396
Size: 32 135 32 542 32 217 32 1
Cuts at: 2396 2428 2660 2692 4648 4680 4722 4754 1373
Size: 32 232 32 1956 32 42 32 2626
Fragments arranged by size:
2626 1956 542 232 217 135 42 32
32 32 32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2572 3351 3597 669
Size: 988 281 634 779 246 3079
Fragments arranged by size:
3079 988 779 634 281 246
HindIII A'AGCT_T
Cuts at: 748 2774 748
Size: 2026 3981
HpaI GTT'AAC
Cuts at: 3597 3597
Size: 6007
KpnI G_GTAC'C
Cuts at: 3332 3332
Size: 6007
MfeI C'AATT_G
Cuts at: 2441 3606 2441
Size: 1165 4842
MluI A'CGCG_T
Cuts at: 5883 5883
Size: 6007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2292 2427 2571 2712 5121 5305 1305
Size: 987 135 144 141 2409 184 2007
Fragments arranged by size:
2409 2007 987 184 144 141 135
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2093 2435 10
Size: 54 1425 343 261 342 3582
Fragments arranged by size:
3582 1425 343 342 261 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2042 2754 3065 3965 4324 4483 518
Size: 648 876 712 311 900 359 159 2042
Fragments arranged by size:
2042 900 876 712 648 359 311 159
MspA1I CmG'CkG
Cuts at: 1606 2232 2469 2692 2818 2913 3423 4100 5248
Size: 626 237 223 126 95 510 677 1148
Cuts at: 5248 5493 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 626 510 245 237 223
126 95
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5455
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6007
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6007
NotI GC'GGCC_GC
Cuts at: 3454 3454
Size: 6007
NspI r_CATG'y
Cuts at: 1132 1229 2101 3365 4910 1132
Size: 97 872 1264 1545 2229
Fragments arranged by size:
2229 1545 1264 872 97
PasI CC'CwG_GG
Cuts at: 1348 3034 1348
Size: 1686 4321
PciI A'CATG_T
Cuts at: 4906 4906
Size: 6007
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2407 3217 1108
Size: 1299 810 3898
Fragments arranged by size:
3898 1299 810
PfoI T'CCnGG_A
Cuts at: 2994 3141 2994
Size: 147 5860
PmeI GTTT'AAAC
Cuts at: 3311 3311
Size: 6007
PmlI CAC'GTG
Cuts at: 3249 3249
Size: 6007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 5589 5621 4076
Size: 32 1481 32 4462
Fragments arranged by size:
4462 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2208 2353 3106 3145 3260 2208
Size: 145 753 39 115 4955
Fragments arranged by size:
4955 753 145 115 39
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6007
PsiI TTA'TAA
Cuts at: 3577 3577
Size: 6007
PspOMI G'GGCC_C
Cuts at: 2397 2935 2397
Size: 538 5469
PstI C_TGCA'G
Cuts at: 830 2337 2817 3018 3359 830
Size: 1507 480 201 341 3478
Fragments arranged by size:
3478 1507 480 341 201
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6007
PvuII CAG'CTG
Cuts at: 2818 2913 2818
Size: 95 5912
SacII CC_GC'GG
Cuts at: 2470 2470
Size: 6007
SalI G'TCGA_C
Cuts at: 1936 2570 3349 1936
Size: 634 779 4594
Fragments arranged by size:
4594 779 634
SanDI GG'GwC_CC
Cuts at: 3260 3260
Size: 6007
SbfI CC_TGCA'GG
Cuts at: 3359 3359
Size: 6007
ScaI AGT'ACT
Cuts at: 4255 5967 4255
Size: 1712 4295
SexAI A'CCwGG_T
Cuts at: 3075 3075
Size: 6007
SfcI C'TryA_G
Cuts at: 826 1045 2333 2813 3014 3355 4490 5171 5362
Size: 219 1288 480 201 341 1135 681 191
Cuts at: 5362 826
Size: 1471
Fragments arranged by size:
1471 1288 1135 681 480 341 219 201
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6007
SmaI CCC'GGG
Cuts at: 1872 2978 3334 1872
Size: 1106 356 4545
Fragments arranged by size:
4545 1106 356
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4111 5012 5274 820
Size: 197 926 2168 901 262 1553
Fragments arranged by size:
2168 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3798 493
Size: 3305 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6007
SphI G_CATG'C
Cuts at: 1132 2101 3365 1132
Size: 969 1264 3774
Fragments arranged by size:
3774 1264 969
SspI AAT'ATT
Cuts at: 5 52 3931 5
Size: 47 3879 2081
Fragments arranged by size:
3879 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6007
StyI C'CwwG_G
Cuts at: 513 1065 2151 2367 2421 3446 513
Size: 552 1086 216 54 1025 3074
Fragments arranged by size:
3074 1086 1025 552 216 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2054 2453 4034 4051 4204 4389 4834
Size: 1255 474 399 1581 17 153 185 445
Cuts at: 4834 325
Size: 1498
Fragments arranged by size:
1581 1498 1255 474 445 399 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4253 5965 96
Size: 274 80 33 51 1443 2276 1712 138
Fragments arranged by size:
2276 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5967 5999 5967
Size: 32 5975
Tth111I GACn'n_nGTC
Cuts at: 1316 2500 1316
Size: 1184 4823
XbaI T'CTAG_A
Cuts at: 3343 3343
Size: 6007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2122 2428 3160 2122
Size: 306 732 4969
Fragments arranged by size:
4969 732 306
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6007
XmaI C'CCGG_G
Cuts at: 1870 2976 3332 1870
Size: 1106 356 4545
Fragments arranged by size:
4545 1106 356
XmnI GAAnn'nnTTC
Cuts at: 4136 4136
Size: 6007
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2671 3815 276
Size: 53 83 186 936 1137 1144 2468
Fragments arranged by size:
2468 1144 1137 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspXI PsrI RsrII SacI SapI SfiI SfoI
SrfI SwaI