Restriction map for
pFN21AB7591
(Circular) (Six-base) MAPSORT of: pFN21AB7591.seq Check: 9857 from: 1 to: 5383
pFN21AB7591 5383 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3193 278
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2726 1937
Size: 789 4594
Acc65I G'GTAC_C
Cuts at: 2586 2704 2586
Size: 118 5265
AclI AA'CG_TT
Cuts at: 1563 2682 3510 3883 1563
Size: 1119 828 373 3063
Fragments arranged by size:
3063 1119 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2215 2384 3446 4830 5098 1632
Size: 583 169 1062 1384 268 1917
Fragments arranged by size:
1917 1384 1062 583 268 169
AfeI AGC'GCT
Cuts at: 2390 2390
Size: 5383
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5186
AflIII A'CryG_T
Cuts at: 1203 4282 5259 1203
Size: 3079 977 1327
Fragments arranged by size:
3079 1327 977
AgeI A'CCGG_T
Cuts at: 4214 4214
Size: 5383
AhdI GACnn_n'nnGTC
Cuts at: 4112 4112
Size: 5383
AleI CACnn'nnGTG
Cuts at: 1166 2108 1166
Size: 942 4441
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2084 1184
Size: 900 4483
AlwNI CAG_nnn'CTG
Cuts at: 2407 2609 3126 4698 2407
Size: 202 517 1572 3092
Fragments arranged by size:
3092 1572 517 202
ApaI G_GGCC'C
Cuts at: 2299 2299
Size: 5383
ApaLI G'TGCA_C
Cuts at: 3440 4596 3440
Size: 1156 4227
ApoI r'AATT_y
Cuts at: 1493 2692 2918 3070 5179 1493
Size: 1199 226 152 2109 1697
Fragments arranged by size:
2109 1697 1199 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3937 160
Size: 872 2905 1606
Fragments arranged by size:
2905 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
AvaI C'yCGr_G
Cuts at: 1870 1943 2116 2298 2313 2708 1870
Size: 73 173 182 15 395 4545
Fragments arranged by size:
4545 395 182 173 73 15
BamHI G'GATC_C
Cuts at: 2713 3080 2713
Size: 367 5016
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2022 2211 2552 2586 2704
Size: 325 217 627 235 189 341 34 118
Cuts at: 2704 4159 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 341 325 235 217 189
118 34
BanII G_rGCy'C
Cuts at: 1391 2299 2702 1391
Size: 908 403 4072
Fragments arranged by size:
4072 908 403
BbeI G_GCGC'C
Cuts at: 2026 2215 2556 2026
Size: 189 341 4853
Fragments arranged by size:
4853 341 189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4412
BbvCI CC'TCA_GC
Cuts at: 2654 2654
Size: 5383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3574 3608 1903
Size: 34 1637 34 3678
Fragments arranged by size:
3678 1637 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3275 4485 3275
Size: 1210 4173
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5383
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3994 136
Size: 107 122 71 631 605 2322 1525
Fragments arranged by size:
2322 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5378 5378
Size: 5383
BlpI GC'TnA_GC
Cuts at: 2370 2800 2370
Size: 430 4953
Bme1580I G_kGCm'C
Cuts at: 1790 2299 3444 4600 1790
Size: 509 1145 1156 2573
Fragments arranged by size:
2573 1156 1145 509
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5383
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2100 2231 3114
Size: 416 53 83 399 24 683 131 883
Cuts at: 3114 4072 442
Size: 958 1753
Fragments arranged by size:
1753 958 883 683 416 399 131 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2276 4043 1239
Size: 1037 1767 2579
Fragments arranged by size:
2579 1767 1037
Bpu10I CC'TnA_GC
Cuts at: 2654 2654
Size: 5383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2681 3508 4373 4671 2681
Size: 827 865 298 3393
Fragments arranged by size:
3393 865 827 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4046 882
Size: 617 2547 2219
Fragments arranged by size:
2547 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3174 493
Size: 711 1970 2702
Fragments arranged by size:
2702 1970 711
BsaBI GATnn'nnATC
Cuts at: 5377 5377
Size: 5383
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2023 2212 2553 3190
Size: 53 83 186 936 490 189 341 637
Cuts at: 3190 3572 4207 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 637 635 490 382 341 189
186 83 53
BsaWI w'CCGG_w
Cuts at: 3816 4214 4488 4635 5046 3816
Size: 398 274 147 411 4153
Fragments arranged by size:
4153 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5158
Fragments arranged by size:
5158 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5383
BseYI C'CCAG_C
Cuts at: 2535 2602 4586 5082 2535
Size: 67 1984 496 2836
Fragments arranged by size:
2836 1984 496 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2169 2261 1163
Size: 573 433 92 4285
Fragments arranged by size:
4285 573 433 92
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2833 3594 4622 1540
Size: 275 186 832 761 1028 2301
Fragments arranged by size:
2301 1028 832 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2125 3444 3529 4600 2125
Size: 1319 85 1071 2908
Fragments arranged by size:
2908 1319 1071 85
BsmI GAATG_Cn'
Cuts at: 2894 2987 2894
Size: 93 5290
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2125 2299 2702 3444 3529 4600 1391
Size: 399 335 174 403 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 403 399 335 174 85
BspEI T'CCGG_A
Cuts at: 5046 5046
Size: 5383
BspHI T'CATG_A
Cuts at: 3272 5002 3272
Size: 1730 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2507 2738 844
Size: 1663 231 3489
Fragments arranged by size:
3489 1663 231
BsrBI CCG'CTC
Cuts at: 1394 1823 3270 1394
Size: 429 1447 3507
Fragments arranged by size:
3507 1447 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3878 4052 65
Size: 1183 2630 174 1396
Fragments arranged by size:
2630 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2612 4027 4214 1700
Size: 110 802 1415 187 2869
Fragments arranged by size:
2869 1415 802 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5383
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5383
BssSI C'ACGA_G
Cuts at: 3443 4455 3443
Size: 1012 4371
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5383
BstBI TT'CG_AA
Cuts at: 4187 4187
Size: 5383
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5383
BstXI CCAn_nnnn'nTGG
Cuts at: 2110 2110
Size: 5383
BstYI r'GATC_y
Cuts at: 1972 2713 3080 3465 3482 4934 5020 5032 5378
Size: 741 367 385 17 1452 86 12 346
Cuts at: 5378 1972
Size: 1977
Fragments arranged by size:
1977 1452 741 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2106 513
Size: 552 939 102 3790
Fragments arranged by size:
3790 939 552 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4644
Fragments arranged by size:
4644 683 56
BtsI GCAGTG_nn'
Cuts at: 2393 2454 2899 3693 3713 2393
Size: 61 445 794 20 4063
Fragments arranged by size:
4063 794 445 61 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5348
DraI TTT'AAA
Cuts at: 2687 2687
Size: 5383
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4390 809
Size: 507 3074 1802
Fragments arranged by size:
3074 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 2614 2830
Size: 54 1425 50 275 18 255 529 216
Cuts at: 2830 3719 5348 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 529 275 255 216 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2830 1537
Size: 275 1018 4090
Fragments arranged by size:
4090 1018 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3313 1353
Size: 1960 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3970 4344 4490 387
Size: 1064 138 2381 374 146 1280
Fragments arranged by size:
2381 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2215 2276 2384 3446 4043 4830 5098
Size: 393 583 61 108 1062 597 787 268
Cuts at: 5098 1239
Size: 1524
Fragments arranged by size:
1524 1062 787 597 583 393 268 108
61
EcoNI CCTnn'n_nnAGG
Cuts at: 2134 2148 2269 2330 2134
Size: 14 121 61 5187
Fragments arranged by size:
5187 121 61 14
EcoO109I rG'GnC_Cy
Cuts at: 2094 2094
Size: 5383
EcoRI G'AATT_C
Cuts at: 2692 3070 2692
Size: 378 5005
FspI TGC'GCA
Cuts at: 3889 3889
Size: 5383
HaeII r_GCGC'y
Cuts at: 1678 2026 2215 2392 2556 4530 1678
Size: 348 189 177 164 1974 2531
Fragments arranged by size:
2531 1974 348 189 177 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4024 4056 4098 4130 1373
Size: 32 135 32 2452 32 42 32 2626
Fragments arranged by size:
2626 2452 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2727 2973 669
Size: 988 281 789 246 3079
Fragments arranged by size:
3079 988 789 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5383
HpaI GTT'AAC
Cuts at: 2973 2973
Size: 5383
KasI G'GCGC_C
Cuts at: 2022 2211 2552 2022
Size: 189 341 4853
Fragments arranged by size:
4853 341 189
KpnI G_GTAC'C
Cuts at: 2590 2708 2590
Size: 118 5265
MfeI C'AATT_G
Cuts at: 2982 2982
Size: 5383
MluI A'CGCG_T
Cuts at: 5259 5259
Size: 5383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2226 4497 4681 1305
Size: 921 2271 184 2007
Fragments arranged by size:
2271 2007 921 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3561
Fragments arranged by size:
3561 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 3341 3700 3859 518
Size: 648 942 1233 359 159 2042
Fragments arranged by size:
2042 1233 942 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2254 2799 3476 4624 4869 1606
Size: 648 545 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 648 545 245
NaeI GCC'GGC
Cuts at: 2614 2614
Size: 5383
NarI GG'CG_CC
Cuts at: 2023 2212 2553 2023
Size: 189 341 4853
Fragments arranged by size:
4853 341 189
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3892
Fragments arranged by size:
3892 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5383
NgoMIV G'CCGG_C
Cuts at: 2612 2612
Size: 5383
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5383
NotI GC'GGCC_GC
Cuts at: 2830 2830
Size: 5383
NspI r_CATG'y
Cuts at: 1132 1229 2741 4286 1132
Size: 97 1512 1545 2229
Fragments arranged by size:
2229 1545 1512 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5383
PciI A'CATG_T
Cuts at: 4282 4282
Size: 5383
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2676 1108
Size: 1568 3815
PfoI T'CCnGG_A
Cuts at: 2675 2675
Size: 5383
PmeI GTTT'AAAC
Cuts at: 2687 2687
Size: 5383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 4965 4997 3452
Size: 32 1481 32 3838
Fragments arranged by size:
3838 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2094 2094
Size: 5383
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5383
PsiI TTA'TAA
Cuts at: 2953 2953
Size: 5383
PspOMI G'GGCC_C
Cuts at: 2295 2295
Size: 5383
PspXI vC'TCGA_Gb
Cuts at: 2116 2116
Size: 5383
PstI C_TGCA'G
Cuts at: 830 2135 2735 830
Size: 1305 600 3478
Fragments arranged by size:
3478 1305 600
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5383
PvuII CAG'CTG
Cuts at: 2254 2254
Size: 5383
SalI G'TCGA_C
Cuts at: 1936 2725 1936
Size: 789 4594
SanDI GG'GwC_CC
Cuts at: 2094 2094
Size: 5383
SbfI CC_TGCA'GG
Cuts at: 2735 2735
Size: 5383
ScaI AGT'ACT
Cuts at: 3631 5343 3631
Size: 1712 3671
SfcI C'TryA_G
Cuts at: 826 1045 2131 2515 2731 3866 4547 4738 826
Size: 219 1086 384 216 1135 681 191 1471
Fragments arranged by size:
1471 1135 1086 681 384 219 216 191
SfoI GGC'GCC
Cuts at: 2024 2213 2554 2024
Size: 189 341 4853
Fragments arranged by size:
4853 341 189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5383
SmaI CCC'GGG
Cuts at: 1872 2315 2710 1872
Size: 443 395 4545
Fragments arranged by size:
4545 443 395
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2116 2660 3487 4388 4650 820
Size: 197 926 173 544 827 901 262 1553
Fragments arranged by size:
1553 926 901 827 544 262 197 173
SnaBI TAC'GTA
Cuts at: 493 3174 493
Size: 2681 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5383
SphI G_CATG'C
Cuts at: 1132 2741 1132
Size: 1609 3774
SspI AAT'ATT
Cuts at: 5 52 3307 5
Size: 47 3255 2081
Fragments arranged by size:
3255 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5383
StyI C'CwwG_G
Cuts at: 513 1065 2004 2822 513
Size: 552 939 818 3074
Fragments arranged by size:
3074 939 818 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2616 3410 3427 3580 3765 4210 325
Size: 1255 1036 794 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1036 794 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3629 5341 96
Size: 274 80 33 51 1443 1652 1712 138
Fragments arranged by size:
1712 1652 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5343 5375 5343
Size: 32 5351
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5383
XbaI T'CTAG_A
Cuts at: 2719 2719
Size: 5383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2673 2673
Size: 5383
XhoI C'TCGA_G
Cuts at: 1943 2116 1943
Size: 173 5210
XmaI C'CCGG_G
Cuts at: 1870 2313 2708 1870
Size: 443 395 4545
Fragments arranged by size:
4545 443 395
XmnI GAAnn'nnTTC
Cuts at: 3512 3512
Size: 5383
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3191 276
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PsrI RsrII SacI
SacII SapI SexAI SfiI SrfI SwaI