Restriction map for
pFN21AB7590
(Circular) (Six-base) MAPSORT of: pFN21AB7590.seq Check: 1036 from: 1 to: 5575
pFN21AB7590 5575 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3385 278
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2918 1937
Size: 981 4594
Acc65I G'GTAC_C
Cuts at: 2896 2896
Size: 5575
AclI AA'CG_TT
Cuts at: 1563 3702 4075 1563
Size: 2139 373 3063
Fragments arranged by size:
3063 2139 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3638 5022 5290 1632
Size: 2006 1384 268 1917
Fragments arranged by size:
2006 1917 1384 268
AfeI AGC'GCT
Cuts at: 2372 2372
Size: 5575
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5378
AflIII A'CryG_T
Cuts at: 1203 2318 4474 5451 1203
Size: 1115 2156 977 1327
Fragments arranged by size:
2156 1327 1115 977
AgeI A'CCGG_T
Cuts at: 4406 4406
Size: 5575
AhdI GACnn_n'nnGTC
Cuts at: 4304 4304
Size: 5575
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2245 2261 1184
Size: 1061 16 4498
Fragments arranged by size:
4498 1061 16
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2348 2380 2348
Size: 32 5543
AlwNI CAG_nnn'CTG
Cuts at: 2856 3318 4890 2856
Size: 462 1572 3541
Fragments arranged by size:
3541 1572 462
ApaI G_GGCC'C
Cuts at: 2039 2214 2596 2039
Size: 175 382 5018
Fragments arranged by size:
5018 382 175
ApaLI G'TGCA_C
Cuts at: 3632 4788 3632
Size: 1156 4419
ApoI r'AATT_y
Cuts at: 1493 2884 3110 3262 5371 1493
Size: 1391 226 152 2109 1697
Fragments arranged by size:
2109 1697 1391 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4129 160
Size: 872 3097 1606
Fragments arranged by size:
3097 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
AvaI C'yCGr_G
Cuts at: 1870 1943 2207 2900 1870
Size: 73 264 693 4545
Fragments arranged by size:
4545 693 264 73
BamHI G'GATC_C
Cuts at: 2905 3272 2905
Size: 367 5208
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 2651 2896 4351 618
Size: 325 217 627 231 633 245 1455 1842
Fragments arranged by size:
1842 1455 633 627 325 245 231 217
BanII G_rGCy'C
Cuts at: 1391 2039 2214 2596 2894 1391
Size: 648 175 382 298 4072
Fragments arranged by size:
4072 648 382 298 175
BbeI G_GCGC'C
Cuts at: 2022 2022
Size: 5575
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4604
BbvCI CC'TCA_GC
Cuts at: 2152 2518 2710 2152
Size: 366 192 5017
Fragments arranged by size:
5017 366 192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2516 2550 3766 3800 1903
Size: 34 579 34 1216 34 3678
Fragments arranged by size:
3678 1216 579 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3467 4677 3467
Size: 1210 4365
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5575
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2528 2628 2649
Size: 107 122 71 631 605 856 100 21
Cuts at: 2649 4186 136
Size: 1537 1525
Fragments arranged by size:
1537 1525 856 631 605 122 107 100
71 21
BglII A'GATC_T
Cuts at: 5570 5570
Size: 5575
BlpI GC'TnA_GC
Cuts at: 2992 2992
Size: 5575
Bme1580I G_kGCm'C
Cuts at: 1790 2039 2214 2589 2596 2654 3636 4792 1790
Size: 249 175 375 7 58 982 1156 2573
Fragments arranged by size:
2573 1156 982 375 249 175 58 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5575
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2804 3306 4264
Size: 416 53 83 399 24 1387 502 958
Cuts at: 4264 442
Size: 1753
Fragments arranged by size:
1753 1387 958 502 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2143 2158 2375 2519 2582 4235 1239
Size: 904 15 217 144 63 1653 2579
Fragments arranged by size:
2579 1653 904 217 144 63 15
Bpu10I CC'TnA_GC
Cuts at: 2152 2518 2710 2152
Size: 366 192 5017
Fragments arranged by size:
5017 366 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3700 4565 4863 3700
Size: 865 298 4412
Fragments arranged by size:
4412 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2076 2426 2487 4238 882
Size: 617 577 350 61 1751 2219
Fragments arranged by size:
2219 1751 617 577 350 61
BsaAI yAC'GTr
Cuts at: 493 1204 2321 3366 493
Size: 711 1117 1045 2702
Fragments arranged by size:
2702 1117 1045 711
BsaBI GATnn'nnATC
Cuts at: 5569 5569
Size: 5575
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2019 2510 3382 3764
Size: 53 83 186 936 486 491 872 382
Cuts at: 3764 4399 275
Size: 635 1451
Fragments arranged by size:
1451 936 872 635 491 486 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4008 4406 4680 4827 5238 4008
Size: 398 274 147 411 4345
Fragments arranged by size:
4345 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2348 2378 1178
Size: 30 165 30 945 30 4375
Fragments arranged by size:
4375 945 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2506 2668 1186
Size: 1320 162 4093
Fragments arranged by size:
4093 1320 162
BseYI C'CCAG_C
Cuts at: 4778 5274 4778
Size: 496 5079
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5002
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2032 3025 3786 4814 1540
Size: 275 186 31 993 761 1028 2301
Fragments arranged by size:
2301 1028 993 761 275 186 31
BsiHKAI G_wGCw'C
Cuts at: 3636 3721 4792 3636
Size: 85 1071 4419
Fragments arranged by size:
4419 1071 85
BsmI GAATG_Cn'
Cuts at: 3086 3179 3086
Size: 93 5482
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2214 2589 2596 2654 2894 3636
Size: 399 249 175 375 7 58 240 742
Cuts at: 3636 3721 4792 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 375 249 240 175
85 58 7
BspEI T'CCGG_A
Cuts at: 5238 5238
Size: 5575
BspHI T'CATG_A
Cuts at: 3464 5194 3464
Size: 1730 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2644 2930 844
Size: 1800 286 3489
Fragments arranged by size:
3489 1800 286
BsrBI CCG'CTC
Cuts at: 1394 1823 2096 2767 3462 1394
Size: 429 273 671 695 3507
Fragments arranged by size:
3507 695 671 429 273
BsrDI GCAATG_nn'
Cuts at: 65 1248 4070 4244 65
Size: 1183 2822 174 1396
Fragments arranged by size:
2822 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2304 2571 2783 4219 4406 1700
Size: 110 494 267 212 1436 187 2869
Fragments arranged by size:
2869 1436 494 267 212 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5575
BssHII G'CGCG_C
Cuts at: 1925 2042 1925
Size: 117 5458
BssSI C'ACGA_G
Cuts at: 3635 4647 3635
Size: 1012 4563
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5575
BstBI TT'CG_AA
Cuts at: 4379 4379
Size: 5575
BstEII G'GTnAC_C
Cuts at: 1184 2663 1184
Size: 1479 4096
BstXI CCAn_nnnn'nTGG
Cuts at: 2823 2823
Size: 5575
BstYI r'GATC_y
Cuts at: 1972 2773 2905 3272 3657 3674 5126 5212 5224
Size: 801 132 367 385 17 1452 86 12
Cuts at: 5224 5570 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 801 385 367 346 132 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2286 2659 2286
Size: 373 5202
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4084
Fragments arranged by size:
4084 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2025 487
Size: 683 56 799 4037
Fragments arranged by size:
4037 799 683 56
BtsI GCAGTG_nn'
Cuts at: 2227 2331 2723 3091 3885 3905 2227
Size: 104 392 368 794 20 3897
Fragments arranged by size:
3897 794 392 368 104 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2121 2156 529
Size: 35 1557 35 3948
Fragments arranged by size:
3948 1557 35 35
DraI TTT'AAA
Cuts at: 2879 2879
Size: 5575
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4582 809
Size: 507 3266 1802
Fragments arranged by size:
3266 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2122 2142 3022
Size: 54 1425 50 275 18 292 20 880
Cuts at: 3022 3911 5540 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 880 292 275 54 50
43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 3022 1537
Size: 275 1210 4090
Fragments arranged by size:
4090 1210 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3505 1353
Size: 2152 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2066 4162 4536 4682 387
Size: 1064 138 477 2096 374 146 1280
Fragments arranged by size:
2096 1280 1064 477 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2143 2158 2375 2519 2582 3638 4235
Size: 393 511 15 217 144 63 1056 597
Cuts at: 4235 5022 5290 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1056 787 597 511 393 268 217
144 63 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2285 2285
Size: 5575
EcoO109I rG'GnC_Cy
Cuts at: 2035 2036 2148 2290 2592 2593 2035
Size: 1 112 142 302 1 5017
Fragments arranged by size:
5017 302 142 112 1 1
EcoRI G'AATT_C
Cuts at: 2884 3262 2884
Size: 378 5197
FspI TGC'GCA
Cuts at: 4081 4081
Size: 5575
HaeII r_GCGC'y
Cuts at: 1678 2022 2077 2374 2612 2850 4722 1678
Size: 344 55 297 238 238 1872 2531
Fragments arranged by size:
2531 1872 344 297 238 238 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4216 4248 4290 4322 1373
Size: 32 135 32 2644 32 42 32 2626
Fragments arranged by size:
2644 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2919 3165 669
Size: 988 281 981 246 3079
Fragments arranged by size:
3079 988 981 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5575
HpaI GTT'AAC
Cuts at: 3165 3165
Size: 5575
KasI G'GCGC_C
Cuts at: 2018 2018
Size: 5575
KpnI G_GTAC'C
Cuts at: 2900 2900
Size: 5575
MfeI C'AATT_G
Cuts at: 3174 3174
Size: 5575
MluI A'CGCG_T
Cuts at: 5451 5451
Size: 5575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2568 4689 4873 1305
Size: 1263 2121 184 2007
Fragments arranged by size:
2121 2007 1263 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2124 10
Size: 54 1425 343 292 3461
Fragments arranged by size:
3461 1425 343 292 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 3533 3892 4051 518
Size: 648 1077 1290 359 159 2042
Fragments arranged by size:
2042 1290 1077 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2091 2344 2419 2698 2744 2823 2856 2991
Size: 485 253 75 279 46 79 33 135
Cuts at: 2991 3668 4816 5061 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 485 279 253 245 135
79 75 46 33
NaeI GCC'GGC
Cuts at: 2573 2785 2573
Size: 212 5363
NarI GG'CG_CC
Cuts at: 2019 2019
Size: 5575
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4084
Fragments arranged by size:
4084 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5575
NgoMIV G'CCGG_C
Cuts at: 2571 2783 2571
Size: 212 5363
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5575
NotI GC'GGCC_GC
Cuts at: 3022 3022
Size: 5575
NspI r_CATG'y
Cuts at: 1132 1229 2933 4478 1132
Size: 97 1704 1545 2229
Fragments arranged by size:
2229 1704 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2589 1348
Size: 1241 4334
PciI A'CATG_T
Cuts at: 4474 4474
Size: 5575
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2383 1108
Size: 1275 4300
PfoI T'CCnGG_A
Cuts at: 2769 2769
Size: 5575
PmeI GTTT'AAAC
Cuts at: 2879 2879
Size: 5575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2348 2380 3644 3676 5157 5189 2348
Size: 32 1264 32 1481 32 2734
Fragments arranged by size:
2734 1481 1264 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2148 2148
Size: 5575
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5575
PsiI TTA'TAA
Cuts at: 3145 3145
Size: 5575
PspOMI G'GGCC_C
Cuts at: 2035 2210 2592 2035
Size: 175 382 5018
Fragments arranged by size:
5018 382 175
PstI C_TGCA'G
Cuts at: 830 2927 830
Size: 2097 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5575
PvuII CAG'CTG
Cuts at: 2344 2419 2744 2823 2856 2344
Size: 75 325 79 33 5063
Fragments arranged by size:
5063 325 79 75 33
SalI G'TCGA_C
Cuts at: 1936 2917 1936
Size: 981 4594
SbfI CC_TGCA'GG
Cuts at: 2927 2927
Size: 5575
ScaI AGT'ACT
Cuts at: 3823 5535 3823
Size: 1712 3863
SfcI C'TryA_G
Cuts at: 826 1045 2923 4058 4739 4930 826
Size: 219 1878 1135 681 191 1471
Fragments arranged by size:
1878 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2020 2020
Size: 5575
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5575
SmaI CCC'GGG
Cuts at: 1872 2209 2902 1872
Size: 337 693 4545
Fragments arranged by size:
4545 693 337
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3679 4580 4842 820
Size: 197 926 1736 901 262 1553
Fragments arranged by size:
1736 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3366 493
Size: 2873 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5575
SphI G_CATG'C
Cuts at: 1132 2933 1132
Size: 1801 3774
SrfI GCCC'GGGC
Cuts at: 2209 2209
Size: 5575
SspI AAT'ATT
Cuts at: 5 52 3499 5
Size: 47 3447 2081
Fragments arranged by size:
3447 2081 47
StuI AGG'CCT
Cuts at: 1517 2531 2631 1517
Size: 1014 100 4461
Fragments arranged by size:
4461 1014 100
StyI C'CwwG_G
Cuts at: 513 1065 2004 3014 513
Size: 552 939 1010 3074
Fragments arranged by size:
3074 1010 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3602 3619 3772 3957 4402 325
Size: 1255 2022 17 153 185 445 1498
Fragments arranged by size:
2022 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3821 5533 96
Size: 274 80 33 51 1443 1844 1712 138
Fragments arranged by size:
1844 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5535 5567 5535
Size: 32 5543
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5575
XbaI T'CTAG_A
Cuts at: 2911 2911
Size: 5575
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5575
XmaI C'CCGG_G
Cuts at: 1870 2207 2900 1870
Size: 337 693 4545
Fragments arranged by size:
4545 693 337
XmnI GAAnn'nnTTC
Cuts at: 3704 3704
Size: 5575
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3383 276
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SwaI XcmI