Restriction map for
pFN21AB7589
(Circular) (Six-base) MAPSORT of: pFN21AB7589.seq Check: 9693 from: 1 to: 5377
pFN21AB7589 5377 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3187 278
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2720 1937
Size: 783 4594
Acc65I G'GTAC_C
Cuts at: 2698 2698
Size: 5377
AclI AA'CG_TT
Cuts at: 1563 3504 3877 1563
Size: 1941 373 3063
Fragments arranged by size:
3063 1941 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3440 4824 5092 1632
Size: 1808 1384 268 1917
Fragments arranged by size:
1917 1808 1384 268
AfeI AGC'GCT
Cuts at: 2288 2288
Size: 5377
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5180
AflIII A'CryG_T
Cuts at: 1203 2328 4276 5253 1203
Size: 1125 1948 977 1327
Fragments arranged by size:
1948 1327 1125 977
AgeI A'CCGG_T
Cuts at: 4208 4208
Size: 5377
AhdI GACnn_n'nnGTC
Cuts at: 4106 4106
Size: 5377
AleI CACnn'nnGTG
Cuts at: 1166 2333 1166
Size: 1167 4210
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5377
AlwNI CAG_nnn'CTG
Cuts at: 2146 2434 3120 4692 2146
Size: 288 686 1572 2831
Fragments arranged by size:
2831 1572 686 288
ApaI G_GGCC'C
Cuts at: 2607 2607
Size: 5377
ApaLI G'TGCA_C
Cuts at: 2324 3434 4590 2324
Size: 1110 1156 3111
Fragments arranged by size:
3111 1156 1110
ApoI r'AATT_y
Cuts at: 1493 2686 2912 3064 5173 1493
Size: 1193 226 152 2109 1697
Fragments arranged by size:
2109 1697 1193 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3931 160
Size: 872 2899 1606
Fragments arranged by size:
2899 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
AvaI C'yCGr_G
Cuts at: 1870 1943 2393 2527 2702 1870
Size: 73 450 134 175 4545
Fragments arranged by size:
4545 450 175 134 73
BamHI G'GATC_C
Cuts at: 2507 2707 3074 2507
Size: 200 367 4810
Fragments arranged by size:
4810 367 200
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2020 2165 2614 2698
Size: 325 217 627 221 12 145 449 84
Cuts at: 2698 4153 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 449 325 221 217 145
84 12
BanII G_rGCy'C
Cuts at: 1391 2379 2470 2607 2696 1391
Size: 988 91 137 89 4072
Fragments arranged by size:
4072 988 137 91 89
BbeI G_GCGC'C
Cuts at: 2012 2024 2169 2012
Size: 12 145 5220
Fragments arranged by size:
5220 145 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4406
BbvCI CC'TCA_GC
Cuts at: 2598 2598
Size: 5377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3568 3602 1903
Size: 34 1631 34 3678
Fragments arranged by size:
3678 1631 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3269 4479 3269
Size: 1210 4167
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5377
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2573 3988 136
Size: 107 122 71 631 605 901 1415 1525
Fragments arranged by size:
1525 1415 901 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5372 5372
Size: 5377
BlpI GC'TnA_GC
Cuts at: 2794 2794
Size: 5377
Bme1580I G_kGCm'C
Cuts at: 1790 2328 2607 2619 3438 4594 1790
Size: 538 279 12 819 1156 2573
Fragments arranged by size:
2573 1156 819 538 279 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5377
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3108 4066 442
Size: 416 53 83 399 24 1691 958 1753
Fragments arranged by size:
1753 1691 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2607 2607
Size: 5377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2225 2579 2686 4037 1239
Size: 968 18 354 107 1351 2579
Fragments arranged by size:
2579 1351 968 354 107 18
Bpu10I CC'TnA_GC
Cuts at: 2598 2598
Size: 5377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3502 4367 4665 3502
Size: 865 298 4214
Fragments arranged by size:
4214 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4040 882
Size: 617 2541 2219
Fragments arranged by size:
2541 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2343 3168 493
Size: 711 1139 825 2702
Fragments arranged by size:
2702 1139 825 711
BsaBI GATnn'nnATC
Cuts at: 5371 5371
Size: 5377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2021 2166 2445
Size: 53 83 186 936 476 12 145 279
Cuts at: 2445 3184 3566 4201 275
Size: 739 382 635 1451
Fragments arranged by size:
1451 936 739 635 476 382 279 186
145 83 53 12
BsaWI w'CCGG_w
Cuts at: 3810 4208 4482 4629 5040 3810
Size: 398 274 147 411 4147
Fragments arranged by size:
4147 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2534 2564 1178
Size: 30 165 30 1131 30 3991
Fragments arranged by size:
3991 1131 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2576 1186
Size: 1390 3987
BseYI C'CCAG_C
Cuts at: 2061 2437 2519 4580 5076 2061
Size: 376 82 2061 496 2362
Fragments arranged by size:
2362 2061 496 376 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2195 1163
Size: 573 459 4345
Fragments arranged by size:
4345 573 459
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2827 3588 4616 1540
Size: 275 186 826 761 1028 2301
Fragments arranged by size:
2301 1028 826 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2328 2379 2552 3438 3523 4594 2328
Size: 51 173 886 85 1071 3111
Fragments arranged by size:
3111 1071 886 173 85 51
BsmI GAATG_Cn'
Cuts at: 2888 2981 2888
Size: 93 5284
BsmBI CGTCTCn'nnnn_
Cuts at: 2060 2060
Size: 5377
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2379 2470 2552 2607 2619 2696
Size: 399 538 51 91 82 55 12 77
Cuts at: 2696 3438 3523 4594 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 538 399 91 85 82
77 55 51 12
BspEI T'CCGG_A
Cuts at: 5040 5040
Size: 5377
BspHI T'CATG_A
Cuts at: 3266 4996 3266
Size: 1730 3647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2732 844
Size: 1888 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3264 1394
Size: 429 1441 3507
Fragments arranged by size:
3507 1441 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3872 4046 65
Size: 1183 2624 174 1396
Fragments arranged by size:
2624 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2297 4021 4208 1700
Size: 110 487 1724 187 2869
Fragments arranged by size:
2869 1724 487 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5377
BssHII G'CGCG_C
Cuts at: 1925 2116 2118 2249 1925
Size: 191 2 131 5053
Fragments arranged by size:
5053 191 131 2
BssSI C'ACGA_G
Cuts at: 2318 3437 4449 2318
Size: 1119 1012 3246
Fragments arranged by size:
3246 1119 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2146 2323 794
Size: 1352 177 3848
Fragments arranged by size:
3848 1352 177
BstBI TT'CG_AA
Cuts at: 4181 4181
Size: 5377
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5377
BstYI r'GATC_y
Cuts at: 1972 2431 2507 2707 3074 3459 3476 4928 5014
Size: 459 76 200 367 385 17 1452 86
Cuts at: 5014 5026 5372 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 459 385 367 346 200 86
76 17 12
Bsu36I CC'TnA_GG
Cuts at: 2571 2571
Size: 5377
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 513
Size: 552 939 12 3874
Fragments arranged by size:
3874 939 552 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4638
Fragments arranged by size:
4638 683 56
BtsI GCAGTG_nn'
Cuts at: 2893 3687 3707 2893
Size: 794 20 4563
Fragments arranged by size:
4563 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5377
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5342
DraI TTT'AAA
Cuts at: 2681 2681
Size: 5377
DraIII CAC_nnn'GTG
Cuts at: 2323 2323
Size: 5377
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2363 4384 809
Size: 507 1047 2021 1802
Fragments arranged by size:
2021 1802 1047 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2026 2824 3713
Size: 54 1425 50 275 18 196 798 889
Cuts at: 3713 5342 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 798 275 196 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2824 1537
Size: 275 1012 4090
Fragments arranged by size:
4090 1012 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3307 1353
Size: 1954 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2288 3964 4338 4484 387
Size: 1064 138 699 1676 374 146 1280
Fragments arranged by size:
1676 1280 1064 699 374 146 138
EcoICRI GAG'CTC
Cuts at: 2377 2377
Size: 5377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2225 2579 2686 3440 4037 4824
Size: 393 575 18 354 107 754 597 787
Cuts at: 4824 5092 1239
Size: 268 1524
Fragments arranged by size:
1524 787 754 597 575 393 354 268
107 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2561 2561
Size: 5377
EcoO109I rG'GnC_Cy
Cuts at: 2531 2566 2603 2531
Size: 35 37 5305
Fragments arranged by size:
5305 37 35
EcoRI G'AATT_C
Cuts at: 2686 3064 2686
Size: 378 4999
FspI TGC'GCA
Cuts at: 3883 3883
Size: 5377
HaeII r_GCGC'y
Cuts at: 1678 2012 2024 2169 2290 2502 4524 1678
Size: 334 12 145 121 212 2022 2531
Fragments arranged by size:
2531 2022 334 212 145 121 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2534 2566 4018 4050 4092
Size: 32 135 32 962 32 1452 32 42
Cuts at: 4092 4124 1373
Size: 32 2626
Fragments arranged by size:
2626 1452 962 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2721 2967 669
Size: 988 281 783 246 3079
Fragments arranged by size:
3079 988 783 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5377
HpaI GTT'AAC
Cuts at: 2967 2967
Size: 5377
KasI G'GCGC_C
Cuts at: 2008 2020 2165 2008
Size: 12 145 5220
Fragments arranged by size:
5220 145 12
KpnI G_GTAC'C
Cuts at: 2702 2702
Size: 5377
MfeI C'AATT_G
Cuts at: 2976 2976
Size: 5377
MluI A'CGCG_T
Cuts at: 5253 5253
Size: 5377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2577 4491 4675 1305
Size: 1272 1914 184 2007
Fragments arranged by size:
2007 1914 1272 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3555
Fragments arranged by size:
3555 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2333 3335 3694 3853 518
Size: 648 1167 1002 359 159 2042
Fragments arranged by size:
2042 1167 1002 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2266 2407 2793 3470 4618 4863 1606
Size: 412 248 141 386 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 412 386 248 245 141
NaeI GCC'GGC
Cuts at: 2299 2299
Size: 5377
NarI GG'CG_CC
Cuts at: 2009 2021 2166 2009
Size: 12 145 5220
Fragments arranged by size:
5220 145 12
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3886
Fragments arranged by size:
3886 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5377
NgoMIV G'CCGG_C
Cuts at: 2297 2297
Size: 5377
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5377
NotI GC'GGCC_GC
Cuts at: 2824 2824
Size: 5377
NspI r_CATG'y
Cuts at: 1132 1229 2317 2735 4280 1132
Size: 97 1088 418 1545 2229
Fragments arranged by size:
2229 1545 1088 418 97
PasI CC'CwG_GG
Cuts at: 1348 2626 1348
Size: 1278 4099
PciI A'CATG_T
Cuts at: 4276 4276
Size: 5377
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5377
PfoI T'CCnGG_A
Cuts at: 2427 2503 2427
Size: 76 5301
PmeI GTTT'AAAC
Cuts at: 2681 2681
Size: 5377
PmlI CAC'GTG
Cuts at: 2343 2343
Size: 5377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3446 3478 4959 4991 3446
Size: 32 1481 32 3832
Fragments arranged by size:
3832 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2531 2531
Size: 5377
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5377
PsiI TTA'TAA
Cuts at: 2947 2947
Size: 5377
PspOMI G'GGCC_C
Cuts at: 2603 2603
Size: 5377
PspXI vC'TCGA_Gb
Cuts at: 2393 2393
Size: 5377
PstI C_TGCA'G
Cuts at: 830 2142 2271 2729 830
Size: 1312 129 458 3478
Fragments arranged by size:
3478 1312 458 129
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5377
PvuII CAG'CTG
Cuts at: 2266 2266
Size: 5377
SacI G_AGCT'C
Cuts at: 2379 2379
Size: 5377
SacII CC_GC'GG
Cuts at: 2019 2019
Size: 5377
SalI G'TCGA_C
Cuts at: 1936 2719 1936
Size: 783 4594
SbfI CC_TGCA'GG
Cuts at: 2142 2729 2142
Size: 587 4790
ScaI AGT'ACT
Cuts at: 3625 5337 3625
Size: 1712 3665
SfcI C'TryA_G
Cuts at: 826 1045 2138 2267 2725 3860 4541 4732 826
Size: 219 1093 129 458 1135 681 191 1471
Fragments arranged by size:
1471 1135 1093 681 458 219 191 129
SfoI GGC'GCC
Cuts at: 2010 2022 2167 2010
Size: 12 145 5220
Fragments arranged by size:
5220 145 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5377
SmaI CCC'GGG
Cuts at: 1872 2529 2704 1872
Size: 657 175 4545
Fragments arranged by size:
4545 657 175
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2393 3481 4382 4644 820
Size: 197 926 450 1088 901 262 1553
Fragments arranged by size:
1553 1088 926 901 450 262 197
SnaBI TAC'GTA
Cuts at: 493 3168 493
Size: 2675 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5377
SphI G_CATG'C
Cuts at: 1132 2317 2735 1132
Size: 1185 418 3774
Fragments arranged by size:
3774 1185 418
SspI AAT'ATT
Cuts at: 5 52 3301 5
Size: 47 3249 2081
Fragments arranged by size:
3249 2081 47
StuI AGG'CCT
Cuts at: 1517 2671 1517
Size: 1154 4223
StyI C'CwwG_G
Cuts at: 513 1065 2004 2672 2816 513
Size: 552 939 668 144 3074
Fragments arranged by size:
3074 939 668 552 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3404 3421 3574 3759 4204 325
Size: 1255 1824 17 153 185 445 1498
Fragments arranged by size:
1824 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3623 5335 96
Size: 274 80 33 51 1443 1646 1712 138
Fragments arranged by size:
1712 1646 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2531 2563 5337 5369 2531
Size: 32 2774 32 2539
Fragments arranged by size:
2774 2539 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5377
XbaI T'CTAG_A
Cuts at: 2713 2713
Size: 5377
XhoI C'TCGA_G
Cuts at: 1943 2393 1943
Size: 450 4927
XmaI C'CCGG_G
Cuts at: 1870 2527 2702 1870
Size: 657 175 4545
Fragments arranged by size:
4545 657 175
XmnI GAAnn'nnTTC
Cuts at: 3506 3506
Size: 5377
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3185 276
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstXI
BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI
PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI
XcmI